운동기간에 따른 혈중지단백대사 및 비만호르몬의 변화

홍성찬,함용기,김영빈,박병근,정동혁,이정윤,권성택 圓光大學校 附設 體力科學硏究所 2003 體力科學硏究 Vol.26 No.2

The purpose of this study is to elucidate the effects of regular swimming exercise on blood lipoprotein metabolism and leptin. In this study, swimming(HRmax 50-75%, 60min/day, 5day/week) was performed and 18 were participated for study during 16 weeks. They were divided into two groups, one group as experimental group(n=9) and the other as control group(n=9). A measured factors were TC, TG, HDL-C, LDL-C and leptin.. All data were expressed as mean± standard deviation by using spss package program(win 9.0), one way anova and independent t-test was used to analyze the difference of exercise duration(0week, 8week, 12week, 16week) between groups. For all statistics performed statistical significance was set at p<.05. After exercise intervention, TC, TG, HDL-C, LDL-C and leptin were found to have more significant effects in the experimental group than control group. This results indicated regular swimming exercise training is beneficial to prevent and treat cardiovascular disease and obesity.

加味牛膝湯이 實驗動物의 鎭痛에 미치는 影響

金炯均,權文鉉,오로사,金成宰,李彦政,李峰根 圓光大學校 韓醫學硏究所 1996 원광한의학 Vol.6 No.1

업무상질병 발생 근로자의 특성 : 1999년 업무상질병으로 요양승인된 사례분석

안연순,강성규,권현길,정호근 大韓産業醫學會 2001 대한직업환경의학회지 Vol.13 No.4

목적 : 이 연구는 1999년 근로복지공단에서 업무상질병으로 인정한 사례를 정밀분석하여 직업병의 종류와 특성을 밝힘으로써 직업병 예방사업이나 산업보건연구의 우선순위를 결정하는데 기초자료로 제공하기 위하여 실시하였다. 방법 : 근로복지공단 주전산망을 통하여 재해일자가 1999년 1월 1일부터 12월 31일까지인 근로자 중2000년 6월 30일까지 요양이 승인된 업무상 질병자2,333명을 파악하고 이 중 뇌심혈관계질환자 1,348명과 근골격계질환자 410명을 제외한 575명을 연구대상으로 하였다. 소속 사업장 또는 연구대상 근로자에 대하여 우편 또는 전화 면담조사를 실시하여 업무상 질병자의 성, 연령, 질병 관련 작업기간, 질병 인지경위, 업종, 직종, 질병종류, 사업장규모 등에 대한 조사를 실시 후 기술분석을 시행하였다. 결과 : 업무상 질병자 575명 중 남성이 511명(88.9%), 여성이 64명 (11.1%)이었다. 사망유무는 조사당시에 사망자가 56명(9.7%)이었고, 사망하지 않은 자가 519명 (90.3%)이었다. 연령별로는50~59세가 217명(37.7%)으로 가장 많았고, 질병관련 근무기간은 10년 이상 근무자의 비율이 49.0%로 가장 높았다. 질병인지경위는 소음성 난청, 진폐증, 이황화탄소 중독으로 인한 업무상 질병자를 제외하고 건강진단을 통하여 질병을 발견한 경우가 조 사자의 12.7%fl 불과하였다. 소속 사업장 규모는5인 미만 사업장이 26.1%로 1999년 당시 산재보험 임의가입 대상인 5인 미만 사업장에 소속된 근로자의 비율이 가장 높았다.질병별로는 호흡기질환이 211명(36.7%)으로 가장 많았고, 다음으로 소음성 난청(이명 1명 포함)149명 (25.9%), 감염성질환 69명(12.0%), 유기용제, 중금속, 가스 등에 의한 중독성질환 60명(10.4%) 순이었다. 기타 피부질환 29명, 암 17명,양성종양(성대결절) 8명, 간장질환 9명, 일사병 및열사병 6명, 신경정신질환 5명, 신경계질환 4명, 안질환 3명, 혈액 ·골수질환 2명, 신장질환 1명, 기타2명이었다. 유해인자별로 분류하였을 때, 분진 209건(36.3%), 물리적인자 157건(27.3%), 화학적인자 89건(16.3%), 생물학적인자 82건(14.3%),작업판련성인자 34건(5.9%), 분류불능 4건으로 분진에 의한 업무상질병의 비율이 가장 높았다.종사업종은 제조업이 262명(45.6%)으로 가장 많았고 다음으로 광업 174명(30.3%), 기타 각종 행정기관 일용직 종사업이 42명 (7.3%), 의료업이 28명 (4.9%)이었다. 직종별로는 기능원 및 관련기능종사자가 285명 (49.6%)으로 가장 많았고 다음으로 장치, 기계조작 및 조립종사자 139명 (24.2%), 단순노무종사자 72명 (12.5%), 전문가 28명 (4.9%), 사무종사자 IS명(2.6%), 기술공 및 준전문가14명(2.4%), 행정 및 경영관리자 8명(1.4%), 판매 및 서비스종사자 8명(1.4%), 미상 6명이었다. 결론 : 이 연구를 통하여 우리나라에서 업무상질병으로 인정받고 있는 사례들을 분석함으로써 기존 통계에서 알 수 없었던 내용을 전체적으로 파악할 수있었고, 인첫받는 사례의 상당부분이 산업의학적 관리의 주요 대상인 팡업이나 제조업 이외의 업종에서 발생하고, 근무조건이 열악한 일용직 근로자에서 발생한다는 사실을 파악하였다. 또, 소음성 난청과 진폐증을 제외하고 업무상질병 승인자의 약 90% 가산업안전보건법상의 건강진단을 통하여 질병을 발견하기보다는 개인적인 병원방문을 통하여 발견한다는 사실을 확인하였다 이것은 작업병을 발견하기 위한특수건강진단 목적중 하나가 제대로 기능을 할 수 없다는 사실을 입증한 것으로 특수건강진단제도이 대한대상자 확대, 검사 항목 확대 등 제도보완이 필요함을 시사하는 결과로 판단된다. 또, 병원근로자, 일용직 근로자 등 업무상질병 위험직종에 대한 업무상질병 예방대책도 조속히 수립되어야 할 것이다. Objectives : The purpose of this study is to analyze the characteristics of occupational diseases listed by Korea Labor Welfare Corporation (KLWC). Methods : Using the database of the KLWC, we collected 575 approved occupational disease cases occurring between 1 January and 31 December 1999 and approved by the thirtieth June in 2000. We investigated the characteristics (sex, age, occupational history, kinds of occupational disease, exposure material, type of enterprises, etc.) of occupational disease using a mail survey and telephone interviews. Results : Men accounted for 88.9 %(511 workers) of the approved cases. 56 cases(9.7 %) died of occupational disease. The most common age group was 50∼59 years of age(n=217, 37.7 %). The proportion of workers with grater than ten years disease related working condition exposure were 195 (49.0 %). The majority of diseases were respiratory problems(211 workers, 22.4%), occupational healing loss(149 workers, 25.9%), infectious disease(69 workers, 12.0%), intoxication(60 workers, 10.4%), skin disease(29 workers, 5.0%) and cancer(17 workers, 3.0%). The causal hazardous agents were dusts(209 cases, 36.3%), physical agents(157 cases, 27.3 %), chemical agents(89 cases, 16.3 %), biological agents(82 cases, 14.3 %) and work-related agents (34 cases, 5.9 %). The major types of enterprise were manufacturing(262 workers, 45.6 %), mining and quarrying(174 workers, 30.3 %), public administration(42 workers, 7.3 %) and the health care Industry(28 workers, 4.9 %). The kinds of job included craft and related trades workers(285 workers, 49.6 %), plant or machine operators and assemblers(139 workers, 24.2 %), elementary occupations(72 workers, 12.5 %) and professionals(28 workers, 4.9 %). Conclusions : We were able to elucidate the kinds of occupational disease and the characteristics of workers through this study. Many approved cases occurred among non-manufacturing and non-mining workers and the kinds of disease were varied. This suggests that management policy must be established to prevent occupational disease occurring among workers in the above type of industries.

젊은 성인에서 신체 만족도에 따른 문제해결양식, 대인관계문제와 우울증상과 자살사고와의 관련성

차승민,김태성,강문희,권명진,김정란,왕성근,지익성 대한생물치료정신의학회 2011 생물치료정신의학 Vol.17 No.2

Objectives:The aim of this study was to evaluate that problem solving styles, interpersonal problems, depression and suicidal ideation according to body image satisfaction. Methods:One hundred and nineteen young healthy normal subjects filled out administered the Body Dysmorphic Disorder Examination-Self Report (Korean version of BDDE-SR K-BDDE-SR), Problem Solving Style Scale(Korean version PSS), Korean Inventory of Interpersonal Problem(KIIP), Korean version of the Depression Anxiety Stress Scale(KDASS-21), Korean version of Beck Scale for Suicidal Ideation(Korean version SSI) and Korean version of Reynolds Suicidal Ideation Questionnaire(Korean version SIQ) for a month. Results:Out of the 119 subjects, 39%(N=46) was male and 61%(N=73) was female. In high K-BDDE-SR group (higher than 67), helplessness was significantly higher than low K-BDDE-SR group(lower than 67), and problem-solving confidence, creative style, approach style were significantly lower than low K-BDDE-SR group. Also, Higher level of all KIIP factors in High K-BDDE-SR group was significantly differ than low K-BDDE-SR group. In High K-BDDE-SR group, K-DASS-21, SSI, and SIQ were significantly higher than low K-BDDE-SR group. Using multiple regression analysis contributing variance toward the total factor score of K-BDDE-SR of participants were K-DASS-21 and SIQ. Conclusion:Our results indicates that high tendency of dissatisfied body images have differences in helplessness, problem-solving confidence, approach style, interpersonal problems, depression and suicidal ideation compared to low tendency of dissatisfied body images. It is necessary to study body dysmorphic disorder patients group in the future.

Poster Session : PS 1457 ; Hemato-Oncology(Oncology) : Prognostic Significance of PDGFR-Beta, VEGF and Ki-67 in Adenoid Cystic Carcinoma

( Seong Yeol Park ),( Soo Jeong Nam ),( Bhumsuk Keam ),( Tae Min Kim ),( Yoon Kyung Jeon ),( Se Hoon Lee ),( Seong Keun Kwon ),( Hun Hah ),( Tack Kyun Kwon ),( Dong Wan Kim ),( Myung Whun Sung ),( Dae 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Although adenoid cystic carcinoma (ACC) is known for slow-growing tumor, once it would metastasized, it became rapidly progressive tumor. Study of molecular markers for risk stratification has been limited due to rare incidence. The purpose of this study was to evaluate potential prognostic factors in ACC. Methods: A total of 76 patients who were diagnosed as ACC at Seoul National University Hospital and available for tumor tissue were analyzed. The clinical information of these patients were reviewed and immunohistochemistry for bFGF, c-kit, FGFR2, FGFR3, MYB, PDGFR-beta, VEGF and Ki-67 were performed. Results: Among the 76 patients, primary sites were salivary gland (51%), nasal cavity or paranasal sinus (25%), oral cavity (9%), lung or trachea (8%), and others (7%). Seventy-one patients (93%) underwent curative operation, fifty-nine patients (78%) treated with radiotherapy and thirteen patients (17%) received palliative chemotherapy. fifty-four patients (71%) for bFGF, fifty-eight patients (76%) for c-kit, seven patients (9%) for FGFR2, five patients (7%) for FGFR3, forty-one patients (54%) for MYB, forty-nine patients (64%) for PDGFR-beta, sixty-nine patients (91%) for VEGF were positive and median Ki-67 value was 5.1% (range 0.3 to 32.6%). In univariate analysis, high expression of PDGFR-beta (HR = 2.76; 95% confidence interval [CI], 1.04 to 7.36; P= 0.042), VEGF (HR = 3.09; 95% CI, 1.07 to 8.94; P = 0.037) and Ki-67 (HR = 5.30; 95% CI, 1.94 to 14.49; P = 0.001) were significantly associated with poor overall survival (OS). In multivariate analysis, PDGFR-beta (P = 0.019), VEGF (P = 0.014) and Ki-67 (P= 0.001) were independently associated with poor OS. Conclusions: High expression of PDGFR-beta, VEGF and Ki-67 were independent poor prognostic factors for OS in ACC.

Polycaprolactone spheres and theromosensitive pluronic F127 hydrogel for vocal fold augmentation: In vivo animal study for the treatment of unilateral vocal fold palsy : PCL/F127 for Injection Laryngoplasty in VFP

Kwon, Seong Keun,Song, Jae-Jun,Cho, Chang Gun,Park, Seok-Won,Choi, Soo Jung,Oh, Se Heang,Lee, Jin Ho Wiley (John WileySons) 2013 The Laryngoscope Vol.123 No.7

<P>The purpose of this study was to explore a novel strategy to restore vocal gap by using polycaprolactone (PCL) spheres with thermosensitive Pluronic F127 in a paralyzed rabbit vocal fold.</P>

Regeneration of Facial Nerve using Mesenchymal Stem Cells in Facial Nerve Palsy Animal Model

( Seong Keun Kwon ),( Jae Jun Song ),( Chang Gun Cho ),( Seok Won Park ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.1

Treatment of the injured peripheral nerve with a long defect remains one of the most difficult problems in nerve reconstructive surgery. Adipose derived mesenchymal stem cells(MSCs) have the potential to differentiate into neuronal and adipose tissue and osteogenic cells. We investigated the effect of MSCs on histological and functional regeneration of facial nerve. After transection of facial nerve in twenty rats, we divided the experimental animals into four groups. In five rats, direct anastomosis was done. In fifteen rats, nerve defect were connected with silicone tubes which were left empty or filled with fibrin or filled with fibrin and MSCs. MSCs were tagged with ferumoxides before transplantation. After 8 weeks, motion of the vibrissae was analyzed and histological examinations were performed. Amplitude and frequency of vibrissae were significantly larger in MSCs group. On H&E and anti-S100 immunohistochemical staining, the maximal diameter of the regenerated nerves was significantly larger in MSCs group. On Prussian blue staining, we could identify the MSCs in the regenerated nerve. We concluded that transplantation of MSCs into a facial nerve gap substantially increased axonal regeneration.

Novel experimental rabbit model of anterior glottic web formation

Kwon, Seong Keun,Kim, Dong Wook,Ryu, Yoon-Jong,Kim, Soo yeon,Chang, Hyun,Sung, Myung Whun,Hah, J. Hun Scandinavian University Press 2015 Acta oto-laryngologica Vol.135 No.8

<P><I>Conclusion:</I> The rabbit model of anterior glottic web (AGW) formation using the laryngofissure technique resulted in reproducible and stable AGW formation that may facilitate research into this area. <I>Objective:</I> To introduce and validate a novel experimental animal model of AGW formation using the rabbit. <I>Methods:</I> The inner larynges of eight New Zealand white rabbits were exposed through the laryngofissure technique. The mucosa of the bilateral true vocal fold was stripped off using the bevel of a needle tip. On the basis of the laryngoscopic findings at 8 weeks postoperatively, the extent of AGW was measured, and the success of this procedure was validated. Laryngeal specimens were sampled at 8 weeks for high-speed recording and histological analysis. <I>Results:</I> In seven (87.5%) rabbits, laryngoscopic examination revealed the formation of a scar band involving the anterior commissure. The mean extent of AGW ratio on the left and right sides was 0.58 ± 0.073 and 0.55 ± 0.075, respectively. The symmetric formation of AGW (<I>p</I> = 0.655, <I>p</I> = 0.128) and stability of the AGW procedure (<I>p</I> = 0.491, left; <I>p</I> = 0.501, right) were statistically validated. On high-speed recording, the vocal mucosal wave was hindered by AGW formation. Histologically, fibro-connective tissue, especially collagen fiber, was observed in the anterior commissure.</P>

The Combined Effect of Autologous Mesenchymal Stem Cells and Hepatocyte Growth Factor on Vocal Fold Regeneration and Fibrosis in Vocal Fold Wound

( Seong Keun Kwon ),( Byung Joo Lee ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.4

Most laryngeal procedures for laryngeal cancer cause vocal fold atrophy and fibrosis, which results in the dysphonia. Adipose derived mesenchymal stem cells(AMSCs) have the potential for multi-lineage differentiation and are easy to isolate. To control the growth and differentiation of stem cells, several growth factors are needed. Some studies have reported that hepatocyte growth factor(HGF) reduces fibrosis in wound healing process. We investigated the effect of AMSCs, given alone or together with HGF, on vocal fold regeneration and fibrosis. AMSCs were isolated and cultured from the ten rabbits. Both sides of the vocal folds were injured by electrocauterization. AMSCs were given to one of the injured vocal folds with hyaluronic acid. In five of ten rabbits, hepatocyte growth factor(HGF) was injected together with the AMSCs. At the other side of the vocal fold in all rabbits, hyaluronic acid was injected. After 8 weeks, laryngoscopic and histological examination showed that the vocal folds injected only with hyaluronic acid became atrophic; however, the vocal folds injected with AMSCs did not demonstrate atrophy. The addition of HGF did not decrease the degree of fibrosis. We concluded that AMSCs can be used to prevent vocal fold atrophy after laryngeal surgery.

Novel High-Throughput DNA Part Characterization Technique for Synthetic Biology

Bak Seong-Kun,Seong Wonjae,Rha Eugene,Lee Hyewon,Kim Seong Keun,Kwon Kil Koang,Kim Haseong,Lee Seung-Goo 한국미생물·생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.8

This study presents a novel DNA part characterization technique that increases throughput by combinatorial DNA part assembly, solid plate-based quantitative fluorescence assay for phenotyping, and barcode tagging-based long-read sequencing for genotyping. We confirmed that the fluorescence intensities of colonies on plates were comparable to fluorescence at the single-cell level from a high-end, flow-cytometry device and developed a high-throughput image analysis pipeline. The barcode tagging-based long-read sequencing technique enabled rapid identification of all DNA parts and their combinations with a single sequencing experiment. Using our techniques, forty-four DNA parts (21 promoters and 23 RBSs) were successfully characterized in 72 h without any automated equipment. We anticipate that this high-throughput and easy-to-use part characterization technique will contribute to increasing part diversity and be useful for building genetic circuits and metabolic pathways in synthetic biology.