충청 서북부 심혈관 질환자의 항산화 영양소 섭취정도 분석

김희선,임혜성 순천향대학교 기초과학연구소 2000 순천향자연과학연구 논문집 Vol.6 No.1

Dietary intakes of vitamin A and C were studied with cardiovascular patients in Chungnam area. Seventy five out-patients(63.3±9.07 years old) with myocardial infarction(MI) history of more than 3 month past were selected and all subjects were gathered at once for cardiac rehabilitation program. Twenty four hour recall was conducted to investigate amounts of dietary vitamin A and C intakes by trained interviewers. The results showed that calorie intake was lower than recommended level for most patients while huge variation was observed in the amount of dietary vitamin A intake. Dietary vitamin C intake was adequate for most patients. When gender comparison was conducted, female patients showed much lower calorie and vitamin A intakes compared to male patients. As age increased, vitamin A intake was decreased while calorie and vitamin C intake did not show any differences. In conclusion, vitamin A and C intakes as well as calorie intake of MI patients in Chungnam area was not adequate. The present data suggested that proper nutritional advice was essential for rehabilitation of MI patients and to improve Patients' health.

대전지역 대학생의 학교 식당 이용실태 및 만족도 조사

왕수경,임영희,윤은영,구난숙,박선미 대전대학교 생활과학연구소 2000 생활과학연구 Vol.6 No.-

The aspects and satisfaction on university foodservice were investigated to provide basic information for better foodservice in university by questionnaires. The survey was conducted in Daejon, and data were analyzed by SAS. Although 87.3% of subjects were under foodservice, only 2.0% of them preferred the foods served in university. The most favorite reason for foodservice was indicated as no need on lunch bag(31.1%) and frendship of classmates(25.5%). The most unfavorite reason for foodservice was indicated as bad taste of foods(68.7%) or many dislike foods(13.2%), also dirty eating place(7.7%). The subject liked ssalbap, baechu kimchi, beef foods. The acceptance about kongbap, yulmu kirnchi, beef products was higher in men than women(p<0.05). The preference of mieokkuk was higher in women and that of beef soup was higher in men(p<0.05). The degree of satisfaction was lower in men than in women about the amount of foods(p<0.001). Also the degree of satisfaction was lower in women than in men about sanitary condition of eating place. 54.6% of them had taken the unwanted materials in foods and 3% of them experienced the deteriorated food. The results suggest that food preference of subjects should be considered in meal planning and various cooking method should be utilized to enhance taste of foods for better foodservice. Also, the importance of various foods intake must be periodically and continuously taught to them through nutrition education.

2,4-D 處理가 'Harcot' 살구와 '美白' 복숭아의 果實 成熟과 品質에 미치는 影響

윤철구,김영호,임상철,김학현,이철희,최관순,김선규 충북대학교 농업과학기술연구소 1999 農業科學硏究 Vol.16 No.-

Effect of 2,4- D application on the fruit maturity and quality of 'Harcot' apricot and 'Mibaek' peach was examined. Leaf area of 'Harcot' apricot was not affected by 2,4-D while that of 'Mibaek' peach was increased by 35mg · L-1 2,4-D. Application of 2,4-D at 35mgㆍL-1 increased the fruit weight of both species, and that of 'Harcot' was doubled. Soluble solids content of 'Harcot' was decreased by 2,4-D while that of 'Mibaek' was not affected. Fruit maturity of 'Harcot' and 'Mibaek' was enhanced for respective 4 and 1 day by 2,4-D application. Percent fruit cracking of 'Harcot' apricot was decreased by 35mg · L-1 2,4-D.

지베렐린과 에세폰의 葉面撒布가 '美白' 복숭아의 熟期와 品質에 미치는 影響

김영호,윤철구,임상철,김학현,이철희,최관순,김선규 충북대학교 농업과학기술연구소 1999 農業科學硏究 Vol.16 No.-

In order to obtain the basic data for artificial maturity control of 'Mibaek' peach, GA and ethephon were foliar sprayed 4 weeks prior to harvest, and their effects on fruit maturity and quality were examined. GA and ethephon tended to decrease the fruit weight except 100mg ㆍ L-1 GA+50 to 100mg · L-1 ethephon treatment. GA increased the fruit firmness, and ethephon at higher concentration increased the fruit drop. Harvest date was advanced for 7 to 8 days by ethephon while was delayed for 6 to 8 days by GA treatment, regardless of concentration.

GA 도포제와 염화칼슘 처리시기가 ‘신고’ 과실의 성숙 및 저장성에 미치는 영향

윤철구(Cheol Ku Youn),김선규(Seon Kyu Kim),임상철(Sang Cheol Lim),김학현(Hag Hyon Kim),김영호(Young Ho Kim),이철희(Cheol Hee1 Lee),최관순(Kwan Soon Choi) 한국원예학회 2000 원예과학기술지 Vol.18 No.2

Electronic Structure of Organic/organic Interface Depending on Heteroepitaxial Growth Using Templating Layer

Hee Seon Lim,Sehun Kim,Jeong Won Kim 한국진공학회(ASCT) 2014 Applied Science and Convergence Technology Vol.23 No.6

The electronic structure at organic-organic interface gives essential information on device performance such as charge transport and mobility. Especially, the molecular orientation of organic material can affect the electronic structure at interface and ultimately the device performance in organic photovoltaics. The molecular orientation is examined by the change in ionization potential (IP) for metal phthalocyanines (MPc, M=Zn, Cu)/fullerene (C60) interfaces on ITO by adding the CuI templating layer through ultraviolet photoelectron spectroscopy measurement. On CuPc/C60 bilayer, the addition of CuI templating layer represents the noticeable change in IP, while it hardly affects the electronic structure of ZnPc/C60 bilayer. The CuPc molecules on CuI represent relatively lying down orientation with intermolecular π-π overlap being aligned in vertical direction. Consequently, in organic photovoltaics consisting of CuPc and C60 as donor and acceptor, respectively, the carrier transport along the direction is enhanced by the insertion of CuI templaing layer. In addition, optical absorption in CuPc molecules is increased due to aligned transition matrix elements. Overall the lying down orientation of CuPc on CuI will improve photovoltaic efficiency.

Biocatalysis and Bioprocess Engineering : Optimization of γ-Aminobutyric Acid Production by Enterococcus faecium JK29 Isolated from a Traditional Fermented Foods

( Hee Seon Lim ),( In Tae Cha ),( Hyunjin Lee ),( Myung Ji Seo ) 한국미생물생명공학회(구 한국산업미생물학회) 2016 한국미생물·생명공학회지 Vol.44 No.1

γ-Aminobutyric acid(GABA)를 생산하는 희귀 젖산균을 분리하기 위하여 전통발효식품으로부터 총 147개의 젖산균을 확보한 후1% 글루탐산 나트륨(L-monosodium glutamate, MSG)를 사용하여 GABA를 생산하는 23개의 균주를 1차 분리하였다. 2차 분리를 위하여 글루탐산 탈탄산효소(glutamate decarboxylase)와 16S rRNA 유전자의 염기서열 분석을 통해 기본 MRS 배지에서 48시간 배양 후 1.56 mM의 GABA를 생산하는 Enterococcus faecium JK29를 최종 분리하였다. E. faecium JK29에 의한 GABA의 생산을 향상시키기 위하여 배양 조건을 최적화하였으며 그 결과 0.5% 자당(sucrose), 2% 효모 추출물(yeast extract), 0.5% 글루탐산 나트륨이 포함된 최적화 MRS 배지를 개발하였다. 최적화 MRS 배지를 활용하여 30oC, pH 7.5에서 48시간 배양을 한 결과 E. faecium JK29이 14.86 mM의 GABA를 생산하는 것을 확인하였다. Dominant lactic acid bacteria (LAB) strains were isolated from traditional fermented foods to obtain rare γ- aminobutyric acid (GABA)-producing LAB. Out of 147 isolates, 23 strains that could produce GABA with 1% (w/v) L-monosodium glutamate (MSG) were first isolated. After further screening of these rare GABA-producing LAB by analysis of the glutamate decarboxylase and 16S rRNA gene sequences, Enterococcus faecium JK29 was isolated, and 1.56 mM of GABA was produced after 48 h cultivation in basic de Man, Rogosa, and Sharpe (MRS) medium. To enhance GABA production by E. faecium JK29, the culture conditions were optimized. When E. faecium JK29 was cultivated in optimized MRS medium containing 0.5% (w/v) sucrose and 2% (w/v) yeast extract with 0.5% (w/v) MSG, GABA production reached 14.86 mM after 48 h cultivation at initial conditions of pH 7.5 and 30℃.

Sarcopenic Obesity: Prevalence and Association With Metabolic Syndrome in the Korean Longitudinal Study on Health and Aging (KLoSHA)

Lim, Soo,Kim, Jung Hee,Yoon, Ji Won,Kang, Seon Mee,Choi, Sung Hee,Park, Young Joo,Kim, Ki Woong,Lim, Jae Young,Park, Kyong Soo,Jang, Hak Chul American Diabetes Association 2010 Diabetes care Vol.33 No.7

<P><B>OBJECTIVE</B></P><P>We investigated the prevalence of sarcopenic obesity (SO) and its relationship with metabolic syndrome in a community-based elderly cohort in Korea.</P><P><B>RESEARCH DESIGN AND METHODS</B></P><P>In this study, 287 men and 278 women aged 65 or older were recruited. Sarcopenia was defined as the appendicular skeletal muscle mass (ASM) divided by height squared (Ht<SUP>2</SUP>) (kg/m<SUP>2</SUP>) or by weight (Wt) (%) of <1 SD below the sex-specific mean for young adults. Obesity was defined as a visceral fat area ≥100 cm<SUP>2</SUP>.</P><P><B>RESULTS</B></P><P>The prevalence of SO was 16.7% in men and 5.7% in women with sarcopenia defined by ASM/Ht<SUP>2</SUP>; however, it was 35.1% in men and 48.1% in women by ASM/Wt. Using ASM/Wt, the homeostasis model assessment of insulin resistance of subjects with SO was higher and they were at higher risk for metabolic syndrome (odds ratio [OR] 8.28 [95% CI 4.45–15.40]) than the obese (5.51 [2.81–10.80]) or sarcopenic group (2.64 [1.08–6.44]).</P><P><B>CONCLUSIONS</B></P><P>SO defined by ASM/Wt was more closely associated with metabolic syndrome than either sarcopenia or obesity alone.</P>

Graphene oxide induces apoptotic cell death in endothelial cells by activating autophagy via calcium-dependent phosphorylation of c-Jun N-terminal kinases

Lim, Mi-Hee,Jeung, In Cheul,Jeong, Jinyoung,Yoon, Sung-Jin,Lee, Sang-Hyun,Park, Jongjin,Kang, Yu-Seon,Lee, Hansu,Park, Young-Jun,Lee, Hee Gu,Lee, Seon-Jin,Han, Baek Soo,Song, Nam Woong,Lee, Sang Chul Elsevier 2016 Acta Biomaterialia: structure-property-function re Vol.46 No.-

<P><B>Abstract</B></P> <P>Despite the rapid expansion of the biomedical applications of graphene oxide (GO), safety issues related to GO, particularly with regard to its effects on vascular endothelial cells (ECs), have been poorly evaluated. To explore possible GO-mediated vasculature cytotoxicity and determine lateral GO size relevance, we constructed four types of GO: micrometer-sized GO (MGO; 1089.9±135.3nm), submicrometer-sized GO (SGO; 390.2±51.4nm), nanometer-sized GO (NGO; 65.5±16.3nm), and graphene quantum dots (GQDs). All types but GQD showed a significant decrease in cellular viability in a dose-dependent manner. Notably, SGO or NGO, but not MGO, potently induced apoptosis while causing no detectable necrosis. Subsequently, SGO or NGO markedly induced autophagy through a process dependent on the c-Jun N-terminal kinase (JNK)-mediated phosphorylation of B-cell lymphoma 2 (Bcl-2), leading to the dissociation of Beclin-1 from the Beclin-1–Bcl-2 complex. Autophagy suppression attenuated the SGO- or NGO-induced apoptotic cell death of ECs, suggesting that SGO- or NGO-induced cytotoxicity is associated with autophagy. Moreover, SGO or NGO significantly induced increased intracellular calcium ion (Ca<SUP>2+</SUP>) levels. Intracellular Ca<SUP>2+</SUP> chelation with BAPTA-AM significantly attenuated microtubule-associated protein 1A/1B-light chain 3-II accumulation and JNK phosphorylation, resulting in reduced autophagy. Furthermore, we found that SGO or NGO induced Ca<SUP>2+</SUP> release from the endoplasmic reticulum through the PLC β3/IP<SUB>3</SUB>/IP<SUB>3</SUB>R signaling axis. These results elucidate the mechanism underlying the size-dependent cytotoxicity of GOs in the vasculature and may facilitate the development of a safer biomedical application of GOs.</P> <P><B>Statement of Significance</B></P> <P>Graphene oxide (GO) have received considerable attention with respect to their utilization in biomedical applications. However, GO-related safety issues concerning human vasculature are very limited. In this manuscript, we report for the first time the differential size-related biological effects of GOs on endothelial cells (ECs). Notably, Subnanometer- and nanometersized GOs induce apoptotic death in ECs via autophagy activation. We propose a molecular mechanism for the GO-induced autophagic cell death through the PLCβ3/IP3/Ca<SUP>2+</SUP>/JNK signaling axis. Our findings could be provide a better understanding of the GO sizedependent cytotoxicity in vasculature and facilitate the future development of safer biomedical applications of GOs.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Economic Threshold for Diamondback Moth (Plutella xylostella (L.) ) on Leaf Broccoli (Brassica oleracea var. botrytis italiana)

Seon-u Choi,Ju-Rak Lim,Ju-Hee Kim,Ki-Kwan Lee,Chang-yun Hwang 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

This study was conducted to develop the economic threshold for the diamondback moth (Plutella xylostella (L.) on Leaf Broccoli (leaf vegetable) in 2007. To investigate the relationship between initial density of diamondback moth larvae and broccoli leaf yield, experimental plots with five treatments (0, 0.5, 1.0, 2.0, 4.0 larvae per plant) as initial density were established. We inoculated larvae in chesses cloth covering to survey larvae density change. When grown to eat, leaves of broccoli were harvested by periods. 60-70% of larvae were removed whenever we picked the leaves. High levels of larvae were associated with significant reductions in leaf yield. There were 85%, 64%, 58%, and 56% yield reductions from the diamond back moth larvae density in 0, 0.5, 1,0, 1.5, 2.0 per plant, respectively 25days after larvae inoculation. The regression equation used to predict leaf yield based on the number of initial larvae density per a plant was y=1635-393x(R2=0.79***). The economic injury level of diamondback moth on leaf broccoli was 2-3 larvae per 10 plants for a damage level of 5%. The economic thresholds was 1-2 larvae per 10 plants. Thus, the diamond back moth management should be initiated 1-2 larvae occurrence per plant.