시간경과에 따른 안정화 이산화염소(Stabilized ClO₂)의 콩팥조직 부패억제에 대한 현미경적 연구

황규성,최기주,백두진,임도선 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.3

소취 및 소독제로서 사용되어온 안정화 이산화염소(S-ClO₂)의 조직에 대한 부패억제 효과를 확인하고자 본 연구를 수행하였다. 실험을 위해 8주령 SD계 흰쥐 콩팥(kidney)을 사용하였고, 안정화 이산화염소를 처리하지 않은 대조군과, 안정화 이산화염소의 분말과 수용액을 처리한 실험군으로 구분하여 광학 및 전자현미경으로 관찰하였다. 광학현미경 관찰 결과, 부패양상은 시간경과에 따라 토리, 보우만주머니 및 세뇨관을 구성하는 세포의 핵과 세포사이 경계가 불분명해지며, 세뇨관의 경우 신장되었다가 결국에 수축되었다. 대조군의 1일군부터 괴사(necrosis)가 시작되어 3일군 이후는 전체적인 조직 괴사로 형태를 구별하기 어려웠다. 실험군에서 3일군의 경우, 조직의 전반적 형태와 괴사정도가 대조군의 1일군과 유사하였다. 전자현미경 관찰 결과, 시간경과에 따라 세포소 기관 및 미토콘드리아의 부분적 붕괴로 시작되어 결과적으로 모든 세포내소기관이 붕괴되었다. 대조군의 1일군에서 세포소기관의 부분적 붕괴가 관찰되었으며, 실험군의 3일군에서 세포소기관 및 미토콘드리아의 부분적 붕괴 현상이 관찰되었다. 대조군의 3일군 이후에서는 세포소기관을 구별할 수 없었다. 이상의 연구 결과에서 37˚C, 습도 80±5%에서 안정화 이산화염소(S-ClO₂)가 부패와 변성을 억제하는 부패억제제로서 효과가 있고, 억제 정도는 실험군의 3일군이 대조군의 1일군에 해당하는 것으로 보아 최소 2일 정도의 부패억제 효과가 있는 것으로 확인되었다. The stabilized ClO₂ gas has been used for many years by the food industry as a strong oxidizing and sanitizing agent that has broad and high biocidal effectiveness. Therefore, “stabilized ClO₂” gas may be used in fields of disinfectant and sterilization. But, there have been few studies on the decomposition-inhibition effect of stabilized ClO₂ gas with passage of time. The main purpose of this study was to examine the decomposition-inhibition effect of stabilized ClO₂ gas and the morphological change of kidney by measuring of the light and electron microscope. Sprague-Dawley (SD) rats weighting from 230 gm to 250 gm were used as experimental animals. Under ether anesthesia, the right kidney of rat was obtained. Put each sample in 37˚C and humidity 80±5% incubator, we obtained each sample after 0 day, 1 day, 2 days, 3 days, 4 days and 5 days. We proceeded the observation of light and electron microscope. The results obtained in this study reveal that stabilized ClO₂ gas is an effective decomposition inhibitor until 2 days that was conducted at 37˚C and humidity 80±5% conditions.

흰쥐 콩팥여과관문의 노화 변화에 관한 투과전자현미경적 연구

이세정,임형수,임도선,황덕호 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.2

콩팥의 모세여과관문은 토리모세혈관, 내피세포, 혈관사이바탕질, 토리바닥막, 발세포로 구성되어 있다. 콩팥의 노화가 일어나게 되면 형태학적 변형이 일어난다고 보고되었는데, 그 중 흰쥐의 경우 생쥐나 사람보다 노화에 따른 토리의 형태가 다양하게 관찰된다는 보고가 있다. 하지만 이런 연구들은 대부분 단면 위주로, 형태변화에 관한 입체적인 연구는 부족한 실정이다. 본 연구에서는 3개월이 지난 성숙한 흰쥐와 24개월이 지난 노화 흰쥐의 토리 모세여과관을 비교하여 그에 따른 형태변화를 광학 및 투과전자현미경을 이용하여 관찰하였으며, 이러한 결과를 바탕으로 연속절편을 통해 컴퓨터 프로그램을 기반으로 한 3차원 재구성을 시행하였다. 그 결과 노화 흰쥐의 토리에서 요공간의 확장, 모세혈관 내피세포의 변형, 토리바닥막의 비대, 혈관사이바탕질의 확장을 관찰할 수 있었다. 또한 3차원 재구성을 시행한 결과 노화 흰쥐의 토리에서 바닥막 경계의 붕괴 현상, 발세포 핵막의 조각화와 분절 양상, 발세포 세포돌기의 부분적인 수축과 세포돌기의 가늘어짐으로 인한 여과틈새의 확장을 관찰할 수 있었다. 이상의 결과로 미루어 볼 때, 노화가 진행되면 토리의 여과관문의 변형으로 인하여 콩팥의 생리학적 역할과 조절이 영향을 받을 것으로 사료된다. The filtration barrier of kidney consists of endothelial cell, glomerular capillary, glomerular basement membrane, mesangial matrix, and podocyte. In aged rats, the morphological changes were shown in various parts, including the glomerulus. These changes were thickening of basement membrane and mesangial matrix, crescent formation of glomerular capillary, deformity of foot processes, glomerular sclerosis and obsolescence. But these glomerular morphologies are partial images or few serial images analysis. In this study, we examined the morphological alteration of glomerulus in the young and aged rats by light microscopy, transmission electron microscopy and three dimensional reconstruction. We were found in aged rat glomerulus, expansion of urinary space and mesangial matrix, thickening and degrading of glomerular basement membrane, decreasing in podocyte foot processes, fragmentation of podocytic nucleus membrane. These observations indicate that may provide useful data for investigating the pathogenesis of age-related dysfunction of kidney.

새만금 간척사업에 따른 갯벌 패류의 군집구조 변화

황선도 ( Hwang Seon Do ),김종식 ( Kim Jong Sig ) 한국수산과학회 2003 한국수산과학회지 Vol.36 No.6

N/A Species composition and distribution patterns of shellfish were investigated in Eoeun and Geojeon tidal flat located in the Saemangeum area on the west coast of Korea from May to October 2000. Nineteen species of shellfish were collected in Geojeon tidal flat. The samples in number of individuals included Umbonium thomasi (90.0%) and Mactra veneriformis (5.0%). In Eoeun tidal flat, ten species of shellfish were collected and the dominant species in number of individuals were Potamocorbula amurensis (55.O%) and U. thomasi (18.6%). These results were compared with previous studies conducted before the beginning of reclamation in Saemangeum. Compared with the results from 1988, a change in species composition was observed. Laternula flexuosa and Nuttallia olivacea appeared, while Mactra chinensis and Coelomactra antiguata disappeared in the deposition area in Geojeon tidal flat. In the erosion area of Eoeun tidal flat, M. veneriformis and Meretrix lusoria appeared, while Cyclina sinensis disappeared. Based on a cluster analysis, the shellfish community in Eoeun tidal flat was classified into three station groups based on sediment types. Geojeon tidal flat was also classified into three station groups. The distribution of shellfish in the Saemangeum area was closely related to the sediment types.

컨테이너선의 Upper Deck 후판부 최적화

황선복(Seon-Bok Hwang),박준석(Jun-Seok Park),이경도(Kyoung-Do Lee),박선환(Seon-Hwan Park) 대한조선학회 2011 대한조선학회 학술대회자료집 Vol.2011 No.11

The longitudinal structural members in way of upper deck of container carrier consist of very thick plates with high tensile strength and high grade, which are of high cost. And also these are the most effective longitudinal strength members. Therefore it is necessary to carefully design the concerned part to minimize the cost. The purpose of this study is to find optimum design of the concerned part in the aspect of cost. ES(Evolution Strategy) technology is applied in this study, and the method to get practically the result is invented. The minimum cost design considering steel materials and/or assembling man power is carried out for existing ships and the effect of the variation of thickness difference, material and labor cost on the result of optimum design is investigated.

Decursin inhibits UVB-induced MMP expression in human dermal fibroblasts via regulation of nuclear factor-κB.

Hwang, Bo-Mi,Noh, Eun-Mi,Kim, Jong-Suk,Kim, Jeong-Mi,Hwang, Jin-Ki,Kim, Hye-Kyung,Kang, Jae-Seon,Kim, Do-Sung,Chae, Han-Jung,You, Yong-Ouk,Kwon, Kang-Beom,Lee, Young-Rae D.A. Spandidos 2013 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.31 No.2

<P>Decursin, a coumarin compound, was originally isolated from the roots of Angelica gigas almost four decades ago, and it was found to exhibit cytotoxicity against various types of human cancer cells and anti-amnesic activity in vivo through the inhibition of AChE activity. However, the anti-skin photoaging effects of decursin have not been reported to date. In the present study, we investigated the inhibitory effects of decursin on the expression of matrix metalloproteinase (MMP)-1 and MMP-3 in human dermal fibroblast (HDF) cells. Western blot analysis and real-time PCR revealed that decursin inhibited the ultraviolet (UV)B-induced expression of MMP-1 and MMP-3 in a dose-dependent manner. Decursin significantly blocked the UVB-induced activation of nuclear factor-κB (NF-κB). However, decursin showed no effect on MAPK or AP-1 activity. In this study, decursin prevented the UVB-induced expression of MMPs via the inhibition of NF-κB activation. In conclusion, decursin may be a potential agent for the prevention and treatment of skin photoaging.</P>

소아 재발성 삼출성 中耳炎에서 Tryptase, Superoxide Dismutase 발현에 관한 연구

도금록,황우준,장철호,박은정,진경선,조재운,강성도,이현 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.5

The purpose of this expriment is to evaluate the antiallergic and antioxidant effects of Kamihyunggyeyungyo-tang on recurrent otitis media with effusion. The author measured the level of Tryptase and Superoxide dismutase from 22 patients of middle ear effusion. Among the 22 patients, 11 patients had been treatad by antibiotics, and the other 11 patients had been treated by Kamihyunggyeyungyo-tang of pediatric recurrent otitis media with effusion(ROME) using Western blot method. In results, the level of tryptase of middle ear effusion and adenoid tissue, the level of Superoxide dismutase of middle ear effusion and adenoid in Kamihyiunggyeyungyo-tang group was significant than that of antibiotics group. Kamihyunggyeyungyo-tang is considered to be appropriate for the treatment of recurrent otitis with effusion and also it has better antiallergic and antioxidant effect than antibiotics.

Signal Compensation for Analog Rotor Position Errors due to Nonideal Sinusoidal Encoder Signals

Seon-Hwan Hwang,Dong-Youn Kim,Jang-Mok Kim,Do-Hyun Jang 전력전자학회 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.1

This paper proposes a compensation algorithm for the analog rotor position errors caused by nonideal sinusoidal encoder output signals including offset and gain errors. In order to achieve a much higher resolution, position sensors such as resolvers or incremental encoders can be replaced by sinusoidal encoders. In practice, however, the periodic ripples related to the analog rotor position are generated by the offset and gain errors between the sine and cosine output signals of sinusoidal encoders. In this paper, the effects of offset and gain errors are easily analyzed by applying the concept of a rotating coordinate system based on the dq transformation method. The synchronous d-axis signal component is used directly to detect the amplitude of the offset and gain errors for the proposed compensator. As a result, the offset and gain errors can be well corrected by three integrators located on the synchronous d-axis component. In addition, the proposed algorithm does not require any additional hardware and can be easily implemented by a simple integral operation. The effectiveness of the proposed algorithm is verified through several experimental results.

Signal Compensation for Analog Rotor Position Errors due to Nonideal Sinusoidal Encoder Signals

Hwang, Seon-Hwan,Kim, Dong-Youn,Kim, Jang-Mok,Jang, Do-Hyun The Korean Institute of Power Electronics 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.1

This paper proposes a compensation algorithm for the analog rotor position errors caused by nonideal sinusoidal encoder output signals including offset and gain errors. In order to achieve a much higher resolution, position sensors such as resolvers or incremental encoders can be replaced by sinusoidal encoders. In practice, however, the periodic ripples related to the analog rotor position are generated by the offset and gain errors between the sine and cosine output signals of sinusoidal encoders. In this paper, the effects of offset and gain errors are easily analyzed by applying the concept of a rotating coordinate system based on the dq transformation method. The synchronous d-axis signal component is used directly to detect the amplitude of the offset and gain errors for the proposed compensator. As a result, the offset and gain errors can be well corrected by three integrators located on the synchronous d-axis component. In addition, the proposed algorithm does not require any additional hardware and can be easily implemented by a simple integral operation. The effectiveness of the proposed algorithm is verified through several experimental results.

간척지에서 청보리 재배를 위한 적정 유기물 및 질소비료 시비량 설정 (초록)

황선웅 ( Seon Woong Hwang ),임석희 ( Seok Hee Lim ),이경도 ( Kyung Do Lee ),강종국 ( Jong Gook Kang ),신평 ( Pyeong Shin ),김성조 ( Seong Jo Kim ),박기혼 ( Ki Hun Park ) 한국토양비료학회 2012 한국토양비료학회 학술발표회 Vol.2012 No.-

만경강과 동진강 유역 간척농경지 토양특성 구분과 농공학적 관리 대책

황선웅(Seon-Woong Hwang),강종국(Jong-Gook Kang),이경도(Kyung-Do Lee),이경보(Kyung-Bo Lee),박기훈(Ki-Hun Park),정덕영(Doug-Young Chung) 한국토양비료학회 2012 한국토양비료학회지 Vol.45 No.3

간척 농경지에 대한 최적 토양관리 방안을 마련코자 새만금 유역 간척농경지를 대상으로 토양의 물리, 화학적 특성을 조사하였다. 새만금 유역의 간척지 유래 토양면적은 총 113,971 ha이며, 이들 지역의 토양통 분포와 토성을 구분해 보면 연대미상 지역은 주로 전남, 부용, 전북통 등 13개 토양통이다. 토성은 미사식양질∼식질이며, 1920년대에서 1960년대에는 만경, 광활, 전북통에 미사사양질이고, 1970년대 이후에는 만경, 광활, 문포, 염포, 포승, 가포, 하사통 등 사질∼사양질 토양이 많았다. 토양화학성은 간척연대와 상관없이 pH와 EC, Exch. K, Mg, Na 농도가 높은 반면 OM은 표토에는 3.3∼16.1 g kg<SUP>-1</SUP>으로 오랜 기간 동안 영농을 했음에도 유기물 함량이 매우 낮았다. 더욱이 심토는 5.6∼1.1g kg<SUP>-1</SUP>으로 유기물이 거의 없는 상태였는데, 이는 수직배수가 불량하고 대형 농기계에 의한 경반층이 형성되어 점토와 유기물 이동이 없었기 때문으로 판단된다. 또한 토양발달 정도를 알 수 있는 집적층 (B층) 형성은 벼를 90년간 재배한 화포 간척지에서만 점토 이동에 따라 표토의 Fe, Mn 성분 등이 지하 20∼30 cm부근에 7∼8 cm 두께로 쌓여 있는데 이러한 원인은 토성이 비교적 점토함량이 높은 미사질식양토라서 가능했던 것 같다. 이들 토양은 염해 우려 토양, 연약지반 토양 및 사질토양이므로 개량은 먼저 지하배수 시설을 하여 지하수위를 낮추어 주고 동시에 석고와 유기물을 시용하여 투수성을 높여 주며, 모래가 많은 사질토양은 점토가 많은 산적토 등을 객토하여야 만 안정적인 작물생산을 기대할 수 있다. The physical and chemical properties of soil in the Mangyeong and Dongjin river basin had been investigated in order to establish the most optimum soil improvement plan on the reclaimed land. The total soil area by reclamation in Saemangeum basin is 113,971 ha. The classification by the distribution of soil series and soil texture is as following. 13 soil series including Chonnam, Buyong and Chonbuk series are period-unknown areas. Regarding the soil texture, they are fine silty ~ clayey very fine. From 1920s to 1960s, Mangyeong, Gwanghwal and Chonbuk series had coarse silty textured soil. After the 1970s, Mangyeong, Gwanghwal, Munpo, Yeompo, Poseung, Gapo and Hasa series have more sandy soil ~ moderately coarse loamy textured soil. Regarding the chemical properties, the concentrations of EC, Exch. K<SUP>+</SUP>, Mg<SUP>2+</SUP>, Na<SUP>+</SUP> and pH are high regardless of the time of reclamation. On the other hand, organic matter (OM) of top soil were 3.3∼16.1 g kg<SUP>-1</SUP>. The organic matter contents were very low though the soil had been farmed for a long time. Furthermore, the deep soil had almost no organic matter with 5.6∼1.1 g kg<SUP>-1</SUP>. The reason is believed that there had not been any movement of OM and clay because pressure or induced pans had been formed by large agricultural machineries and poor vertical drain. Regarding the forming of illuvial horizon (B layer) which tells the development extent of soil, only in the Hwapo reclaimed area where rice had been cultivated for past 90 years, Fe and Mn from top soil are deposited at underground 20~30 cm with 7~8 cm thickness by the movement of clay. It is believed that it had been possible because the earthiness is silty clay loam soil with relatively high content of clay. The soils are soil with concern of damage from sea water, soil on flimsy ground and sandy soil. Therefore, soil improvement for stable crop production can be expected; if the water table would be lowered by subsurface drainage, the water permeability would be enhanced by gypsum and organic matter, and the sandy soil would be replaced by red soil with high content of clay.