혈관평활근세포에서 산화에너지대사 억제에 의한 아밀로이드전구단백질 대사의 변화

한문구,최웅,김헌식,안희열,한설희 대한치매학회 2002 Dementia and Neurocognitive Disorders Vol.1 No.1

Background: A reduction in the activity of cytochrome c oxidase(COX) has been recently identified in mitochondria from platelets and postmortem brain tissue of AD patients Sodium azide (NaN₃). a COX inhibitor, is an effective chemical agent producing energy shortage and oxidative stress both in vitro and in vivo system Furthermore it has been suggested that vascular compromise could be either directly involved AD pathogenesis or indirectly associated with triggering pathogenetic events leading to AD This study was performed to investigate amyloid precursor protein (APP) metabolism by inhibition of mitochondrial energy metabolism in cultured vascular smooth muslce cells (VSMCs) Materials and Methods: VSMCs isolated from the aorta of seven weeks old Spraque-Dawley rat were treated with NaN₃in a low concentration (100-500μM) or in a high concentration (1-100mM) Cellular proliferation and viability were determined by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenly)-2H-tetrazolium) assay Cellular APP was detected with N-terminal specific antibody 22C11. Celldeath was determined by observation of morphology and terminal deoxynucleotidyl transferase-mediated dUTP-fluorescein nick end labeling stain (TUNEL) We used ginkgo biloba extract(EGb761) and melatonin as anti-oxidants to investigate the mechanism of latered APP metabolism Results: The viability of VSMCs was increased after treatment with 1 mM and 10mM NaN₃(p<0.05) unitl 3 hr and then dimnished Many TUNEL positive cells were found in 10mM and 100mM treatment group. but were not apoptotic in nature 22C11 immunoreactivity was not changed at 3 hr, 6hr, 12hr Anti-oxidants reduced cellular proliferation (p<0.05). but did not block TUNEL positivities and did not influence the 22C11 immunoreactivity In a low concentration NaN₃ treatment group the viability of VSMCs was increased concentration dependently(p<0.05) Immunoblot with 22C11 showed the concentration dependent decrease at 145 kDa, 125 kDa. and high molecular weight range (>160kDa) TUNEL staining showed DNA fragmentations and condensations of nuclear chromatin suggesting apoptosis After treatment with anti-oxidants, the cellular proliferation was more decreased (p<0.05), and TUNEL positive cell deaths were blocked Immunoreactivities of 125 kDa (immature APP). 145 kDa (mature APP). and higher molecular weight bands were recovered below 400μM of NaN₃ Immunoreactivity of 145 kDa was recovered in 100 μM NaN₃ treated group Conclusions: The presumed mechanism of low concentration COX inhibitor is the overproduction of reactive oxygen species resulting from a depression of the mitochondiral electron transport chain. whereas potential consequence of high concentration COX inhibitor might be related to decreassion of ATP synthesis and bioenergetic impairment Reactive oxygen radicals in response to low concentration COX inhibitor alter the processing of APP in VSMCs This investigation demonstrated analtered APP metabolism as a peripheral marker of AD Therefore VSMCs treated with low concentration COX inhibitor could be concsidered as a novel in vitro model of AD.

열공형과 비열공형 피질하 혈관성 치매에서 위험인자의 차이에 관한 비교 연구

배희준,정지향,유경호,나덕렬,김상윤,최경규,양동원,손의주,이상도,김재우,박경원,김응규,이재홍,박미영,한일우,함동석,최문성,하충건,최성혜,이애영,이병철,한설희 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.2

Backgrounds and Objectives: Vascular dementia is a group of dementing disoders arising from various stroke syndrome. Among these. subcortical ischemic vascular dementia (SIVD) is regarded as a relatively distinct clinical entity. However, MRI patterns of SIVD are not homogenous. In some patients, lacunes are dominant, and in others, subcortical white matter changes are. This study was designed to compare risk factor profiles between SIVD with and without multiple lacunes. Methods: We divided 47 subjects (22 males, mean age. 68 years) recruited from VADAPET (Multicenter Trial For Evaluation Of The Changes In the PET Images Of Subcortical Vascular Dementia Patient) study into two groups one with more than 5 lacunes in deep gray matter (lacune group) and the other with 5 or less(non-lacune group) Clinical characteristics and laboratory findings of two groups were compared. Results: Nineteen of 47 patients (40%) belonged to the lacune group. The lacune and non-lacune groups d d not differ in the following variables: age, hypertension, diabetes mellitus, hyperlipidemia heart disease, history of stroke or TIA, history of trauma or major surgery, family history of hypertension stroke, or dementia, age at diagnosis of dementia, body mass index, white blood cell count, ESR, CRP, fibrinogen, hemoglobin A1C, total cholesterol. LDL cholesterol creatinine, proteinuria, glucosuria, and microhematuria. However, male sex, smoking alcohol. hemoglobin, and HDL cholesterol were possibly associated more with lacune group SIVD than with non-lacune group (p<0 1) Multivariate analyses revealed that smoking, hemoglobin, and HDL cholesterol were independent predictors of SIVD with multiple lacunes Conclusion: Our study suggests that SIVD with multiple lacunes may be significantly different in smoking habits hemoglobin, and HDL cholesterol from SIVD without multiple lacunes.

(AgCu)_0.43-Au_0.54Pd_0.03 합금의 시효경화 특성

배동화,설효정,이희경,김형일,김교한 대한치과기재학회 2001 대한치과재료학회지 Vol.28 No.3

팔라디움이 함유된, 시효경화성 치과주조용 고금합금의 연구를 위한 기초자료를 얻기 위하여, 4원계 □ 합금의 등온시효경화 거동과 상변태를 경도 시험, X선회절 실험으로 조사하여, 다음과 같은 결과를 얻었다. 본 합금을 250~350℃에서 등온시효처리하면, 면심정방구조를 갖는 준안정상 AuCu Ⅰ'형 규칙상이 면심정방구조를 갖는 AuCu Ⅰ형 규칙상과 면심입방구조를 갖는 Ag-rich a₁상이 생성되기 전에 생성되었다. 본 합금은 시효처리 온도에 따라 다른 시효경화 거동을 보여주었다. 350℃에서 시효처리를 하면, 경도는 시효 초기에 상승하지 않았고, 최고 경도에 도달하여 유지되고나서 시효시간을 연장함에 따라 경도가 급격히 저하하였다. 250℃에서 시효처리를 하면, 경도는 시효 초기부터 뚜렷히 상승하기 시작하였다. 그 후, 약간의 경도 하강을 보이고나서 경도는 다시 서서히 상승을 지속하였다. 350℃에서 경화는 준안정상 AuCu Ⅰ'형 규칙상의 균일핵생성에 기인하였다. 낮은 시효온도에서의 초기 경화는 다른 상변태에 의해 초래되었고, 이어서 일어나는 경화는 준안정상 AuCu Ⅰ'형 규칙상의 생성에 기인하였다. 시효시간을 연장함에 따라 생기는 과시효 연화는 평형상 AuCu Ⅰ형 규칙상의 생성에 기인하는 것으로 생각된다.

Response of osteoblast-like cells cultured on zirconia to bone morphogenetic protein-2

Han, Seung-Hee,Kim, Kyoung-Hwa,Han, Jung-Seok,Koo, Ki-Tae,Kim, Tae-Il,Seol, Yang-Jo,Lee, Yong-Moo,Ku, Young,Rhyu, In-Chul Korean Academy of Periodontology 2011 Journal of Periodontal & Implant Science Vol.41 No.5

Purpose: The aim of this study was to compare osteoblast behavior on zirconia and titanium under conditions cultured with bone morphogenetic protein-2. Methods: MC3T3-E1 cells were cultured on sandblasted zirconia and sandblasted/etched titanium discs. At 24 hours after seeding MC3T3-E1, the demineralized bone matrix (DBM) gel alone and the DBM gel with bone morphogenetic protein-2 (BMP-2) were added to the culture medium. The surface topography was examined by confocal laser scanning microscopy. Cellular proliferation was measured at 1, 4, and 7 days after gel loading. Alkaline phosphatase activity was measured at 7 days after gel loading. The mRNA expression of ALPase, bone sialoprotein, type I collagen, runt-related transcription factor 2 (Runx-2), osteocalcin, and osterix were evaluated by real-time polymerase chain reaction at 4 days and 7 days. Results: At 1, 4, and 7 days after loading the DBM gel alone and the DBM gel with BMP-2, cellular proliferation on the zirconia and titanium discs was similar and that of the groups cultured with the DBM gel alone and the DBM gel with BMP-2 was not significantly different, except for titanium with BMP-2 gel. ALPase activity was higher in the cells cultured with BMP-2 than in the other groups, but there was no difference between the zirconia and titanium. In ALPase, bone sialoprotein, osteocalcin, Runx-2 and osterix gene expression, that of cells on zirconia or titanium with BMP-2 gel was much more highly increased than titanium without gel at day 7. The gene expression level of cells cultured on zirconia with BMP-2 was higher than that on titanium with BMP-2 at day 7. Conclusions: The data in this study demonstrate that the osteoblastic cell attachment and proliferation of zirconia were comparable to those of titanium. With the stimulation of BMP-2, zirconia has a more pronounced effect on the proliferation and differentiation of the osteoblastic cells compared with titanium.

Acute and 28-Day Subacute Toxicity Studies of Hexane Extracts of the Roots of Lithospermum erythrorhizon in Sprague-Dawley Rats

Han, Chung-Tack,Kim, Myoung-Jun,Moon, Seol-Hee,Jeon, Yu-Rim,Hwang, Jae-Sik,Nam, Chunja,Park, Chong-Woo,Lee, Sun-Ho,Na, Jae-Bum,Park, Chan-Sung,Park, Hee-Won,Lee, Jung-Min,Jang, Ho-Song,Park, Sun-Hee,H Korean Society of ToxicologyKorea Environmental Mu 2015 Toxicological Research Vol.31 No.4

Lithospermum erythrorhizon has long been used as a traditional oriental medicine. In this study, the acute and 28-day subacute oral dose toxicity studies of hexane extracts of the roots of L. erythrorhizon (LEH) were performed in Sprague-Dawley rats. In the acute toxicity study, LEH was administered once orally to 5 male and 5 female rats at dose levels of 500, 1,000, and 2,000 mg/kg. Mortality, clinical signs, and body weight changes were monitored for 14 days. Salivation, soft stool, soiled perineal region, compound-colored stool, chromaturia and a decrease in body weight were observed in the extract-treated groups, and no deaths occurred during the study. Therefore, the approximate lethal dose (ALD) of LEH in male and female rats was higher than 2,000 mg/kg. In the subacute toxicity study, LEH was administered orally to male and female rats for 28 days at dose levels of 25, 100, and 400 mg/kg/day. There was no LEH-related toxic effect in the body weight, food consumption, ophthalmology, hematology, clinical chemistry and organ weights. Compound-colored (black) stool, chromaturia and increased protein, ketone bodies, bilirubin and occult blood in urine were observed in the male and female rats treated with the test substance. In addition, the necropsy revealed dark red discoloration of the kidneys, and the histopathological examination showed presence of red brown pigment or increased hyaline droplets in the renal tubules of the renal cortex. However, there were no test substance-related toxic effects in the hematology and clinical chemistry, and no morphological changes were observed in the histopathological examination of the kidneys. Therefore, it was determined that there was no significant toxicity because the changes observed were caused by the intrinsic color of the test substance. These results suggest that the no-observed-adverse-effect Level (NOAEL) of LEH is greater than 400 mg/kg/day in both sexes.

[P10-35] Effect of Ulmi Cortex Extract on the Recovery of Immune Cells in Whole Body γ- Irradiated Mice

Seol-Hee Han,Hae-Ran Park,Uhee Jung,Sung-Kee Jo 한국식품영양과학회 2005 한국식품영양과학회 학술대회발표집 Vol.2005 No.10

경관지수를 이용한 산림청 지정 명산(名山)과 일반 산의 산림경관구조 비교분석

한희 ( Hee Han ),송정은 ( Jung Eun Song ),설아라 ( A Ra Seol ),박진한 ( Jin Han Park ),정주상 ( Joo Sang Chung ) 한국지리정보학회 2011 한국지리정보학회지 Vol.14 No.1

본 연구에서는 산림청에서 지정한 ``우리나라 100대 명산``에 포함된 국·도립공원 산림과 그 외의 일반산림과의 비교를 통해 산림의 경관구조적 특성을 분석하고자 하였다. 이를 위해 전국의 산림을 대상으로 100대 명산에 지정된 산림 및 지정되지 못한 산림을 각각 34개씩 선정하고, 각 산림에 대해 FRAGSTATS을 활용하여 경관구조에 따른 공간분석 결과를 경관지수로 계량화한 후, 두 집단 간 경관지수의 비교분석을 통해 산림의 경관인식에 영향을 주는 경관구조적 특징들을 찾고자 하였다. 비교분석에 의한 연구결과 국·도립공원 산림은 대체로 일반산림에 비해 평균 경사도가 크고, 해발이 높으며, 지형 기복도가 높은 곳에 위치하는 것으로 나타났다. 또한 국·도립공원 산림은 대체로 수종분포 특성에 따른 영급패치의 형태, 크기 및 복잡성 등이 상대적으로 높은 것으로 나타났다. 이러한 비교분석 결과를 토대로 판단할 때 임분 패치의 평균크기, 경관면적비율, 가장자리밀도 및 다양성 등의 경관구조적 특징들이 산림의 경관인식에 영향을 미칠 수 있는 인자로 판단된다. The objective of this study was to investigate the landscape structures of the National and Provincial Forest Parks in the list of "100 Famous Forests of Korea" designated by Korea Forest Service by comparing them with those of the non-designated forests. Among the designated and the non-designated, 34 mountain forests were chosen respectively over all provinces of Korea. The spatial characteristics of forest landscapes were quantified as the landscape indices independently using FRAGSTATS and the two sets of results of analyses were compared each other. According to the results of the comparative study, the designated forests were found on the higher elevation with the higher average slope and the more complicated relief conditions rather than those of the non-designated. In terms of landscape structure, the designated forests show the larger average patch size, the lower edge density and the higher diversity of landscape components. These results indicate that the more hilly mountain forests with the more complicated spatial distribution patterns of patches are the characteristics of the designated forests. The indices of the forest landscape structure would be useful in understanding the perception of forest landscape.

골프 프로-암에 참여하는 여자프로선수들의 인식

한설희(Han, Seol-Hee),김영재(Kim, Young-Jae) 한국체육과학회 2016 한국체육과학회지 Vol.25 No.3

In this study, we focused on identifying the awareness of female professional golfers participating in Pro-Am when they experience Pro-Am which is held before the golf tour by conducting in-depth interviews with golfers of the Korea Ladies Professional Association(KLPGA). To achieve the goals of this study, we conducted in-depth interviews with 10 female professional golfers with over five years of experience in their careers who have participated in Pro-Am at home and abroad more than 50 times. Golf Pro-Am is an event which is held the day before the golf tour is held and amateur golfers and that professional golfers play the game in a team. Pro-Am of the Korea Ladies Professional Association (KLPGA) started 4 events in 1990 and grew to 19 events in 2014 and the number of golf tours and Pro-Am has steadily increased. However, there are not enough studies on Pro-Am currently. In this study, we studied the awareness of female professional golfers participating Pro-Am. The research result indicated that female professional golfers have two different parts of awareness which are positive awareness and negative awareness on participating Pro-Am. It showed that positive awareness is the pride in participating Pro-Am and diverse opportunities of personal connections while negative awareness is physical burdens and ignorance about amateur golfers(VIP). In this study, there were limits on identifying what the experiences and awareness of female golfers on Pro-Am are even though we conducted in-depth interviews with female golfers. Therefore, we need to analyze the internally psychological factors and externally psychological factors in future studies.

알까기 로봇(R-bot)의 설계

설희승(Hee Seung Seol),김준희(Jun Hee Kim),김영헌(Yeong Heon Kim),조수한(Su Han Jo),주다훤(Da Hwon Ju),김성걸(Seong Keol Kim) 한국생산제조학회 2020 한국생산제조학회지 Vol.29 No.3

상처 치료용 하이드로겔에 적용을 위한 방부제의 세포독성평가

한설희 ( Seol Hee Han ),권희정 ( Hui Jeong Gwon ),임윤묵 ( Youn Mook Lim ),안성준 ( Sung Jun An ),윤민호 ( Min Ho Youn ),조선영 ( Sun Young Jo ),문은경 ( Eun Gyeng Mun ),노영창 ( Young Chang Nho ) 한국공업화학회 2009 응용화학 Vol.13 No.1

Phenoxyethanol과 Paraben류는 항균제로서 제약과 화장품, 식품 안에 공통적으로 사용된다. 우리는 상처 치료용 겔의 부패 방지를 목적으로 phenoxyethanol, methyl paraben, propyl paraben 그리고 이들의 혼합물 사용을 위해 유효한 농도를 결정하고자 세포 독성 실험을 수행하였다. 세포독성 실험은 상기의 방부제를 mouse fibroblast cell line(L929)에 농도별로 처리하여 세포의 생존률로써 확인하였다. 이 결과를 바탕으로 phenoxyethanol, methyl paraben, propyl paraben 그리고 이들의 혼합물을 처리시 E.coli와 S.aureus의 성장 억제 활성을 확인하여 상처 치료용 겔에 적용하고자 한다. Phenoxyethanol and Paraben derivatives are well known as preservative and used in cosmetics and pharmaceutics and foods. Therefore, an investigation was carried out to assess the effect on the cytotoxicity of those preservatives when the preservatives were subjected to a commercial sterilization procedure using gamma-irradiation. The cytotoxicity tests of these hydrogels were evaluated by cell viability of L929 (mouse fibroblast). As results, the preservatives have a good cell viability results.