High-resolution X-ray refraction imaging of rat lung and histological correlations

Jheon, Sanghoon,Youn, Hwa-Shik,Kim, Hong-Tae,Choi, Gi-Hwan,Kim, Jong-Ki Wiley Subscription Services, Inc., A Wiley Company 2006 Microscopy research and technique Vol.69 No.8

<P>This study was performed to observe microstructures of the rat lung, using a synchrotron radiation beam and to compare findings with histological observations. X-ray refraction images from ex-vivo ventilating rat lung were obtained with an 8 KeV monochromatic beam and 20-μm thick CsI(Tl) scintillation crystal. The visual image was magnified using a 20× microscope objective and captured using an analog CCD camera. Obtained images were compared with conventional light microscopic findings from the same tissue. Pulmonary microstructures, including alveolar ducts, alveolar sacs, alveoli, alveolar walls, and perialveolar capillary networks were clearly identified with spatial resolution of as much as 1.2 μm and had good correlation with conventional light microscopic findings. The shape of alveoli appeared more round in SR images than in the light microscopic images. The results suggest that X-ray microscopy study of the lung using synchrotron radiation demonstrates the potential for clinically relevant microstructure of lung tissue without sectioning and fixation. Microsc. Res. Tech., 2006. © 2006 Wiley-Liss, Inc.</P>

Effect of Adjuvant Chemotherapy after Complete Resection for Pathologic Stage IB Lung Adenocarcinoma in High-Risk Patients as Defined by a New Recurrence Risk Scoring Model

Jang, Hyo Joon,Cho, Sukki,Kim, Kwhanmien,Jheon, Sanghoon,Yang, Hee Chul,Kim, Dong Kwan Korean Cancer Association 2017 Cancer Research and Treatment Vol.49 No.4

<P><B>Purpose</B></P><P>We conducted a retrospective analysis to determine if adjuvant chemotherapy prolongs overall survival in patients with pathologic stage IB lung adenocarcinoma who had undergone complete resection and were defined as high-risk by a newly developed recurrence risk scoring model.</P><P><B>Materials and Methods</B></P><P>Patients who underwent curative resection for stage IB lung adenocarcinoma were analyzed with a newly developed recurrence risk scoring model and divided into a low-risk group and a high-risk group. The patients in the high-risk group were retrospectively divided into two groups based on whether they underwent adjuvant chemotherapy or observation. Recurrence-free survival and overall survival were compared between these two groups.</P><P><B>Results</B></P><P>A total of 328 patients who underwent curative resection between 2000 and 2009 were included in this study, of whom 110 (34%) received adjuvant chemotherapy and 218 (67%) underwent observation without additional treatment. According to our risk model, 167 patients (51%) were high-risk and 161 (49%) were low-risk. The 5-year recurrence-free survival rates and overall survival were 84.4% and 91.5% in low-risk patients and 53.9% and 74.7% in high-risk patients (p < 0.001). In high-risk patients, the 5-year overall survival rates were 77% among patients who underwent observation and 87% among those who underwent adjuvant chemotherapy (p=0.019).</P><P><B>Conclusion</B></P><P>Adjuvant chemotherapy prolonged overall survival among high-risk patients who had undergone complete resection for stage IB lung adenocarcinoma.</P>

Epidermal Growth Factor Receptor Mutation and p53 Overexpression during the Multistage Progression of Small Adenocarcinoma of the Lung

Yoo, Seol Bong,Chung, Jin-Haeng,Lee, Hyun Ju,Lee, Choon-Taek,Jheon, Sanghoon,Sung, Sook Whan Elsevier 2010 JOURNAL OF THORACIC ONCOLOGY Vol.5 No.7

Poster Session : PS-1544 ; COPD : Effectiveness of Bronchoscopic Lung Volume Reduction Using Unilateral Endobronchial Valve: A Systematic Review and Meta-Analysis

( Miyoung Choi ),( Worl Suk Lee ),( Min Lee ),( Kyeongman Jeon ),( Seungsoo Sheen ),( Sanghoon Jheon ),( Young Sam Kim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Bronchoscopic lung volume reduction (BLVR) can be suggested as an alternative for surgical lung volume reduction surgery for severe emphysema patients. This article intends to evaluate the safety and effectiveness of BLVR using one-way endobronchial valve by systematic review. Methods: A systematic search of electronic databases including MEDLINE, EMBASE, and Cochrane Library, as well as eight domestic databases up to December 2013 was performed. Two reviewers independently screened all references according to selection criteria. The Scottish Intercollegiate Guidelines Network (SIGN) criterion was used to assess quality of literature. Data from randomized controlled trials (RCTs) were combined and meta-analysis was performed. Results: This review included 15 studies. The forced expiratory volume in one second (FEV1) improved in the intervention group compared to the control group (MD=6.71, 95% CI: 3.31 to 10.11). Six minute walking distance (MD=15.66, 95% CI : 1.69 to 29.64) and cycle workload (MD= 4.43, 95% CI: 1.80- to7.07) also improved. In addition, the St. George`s Respiratory Questionnaire (SGRQ) score decreased (MD -4.29, 95% CI: -6.87 to -1.71) in the intervention group. In a subgroup analysis of patients with complete fissure, the FEV1 change from baseline was higher in the BLVR group than the control group for both 6 month (MD=14.75, p <.001) and 12 months (MD=17.43, p <.001) whereas patients with incomplete fissure the FEV1 and 6MWD showed no change. One year follow-up randomized controlled trials reported deaths although the cause of death was not related to BLVR. Respiratory failure and pneumothorax incidence rate was relatively higher in the BLVR group but the difference was not significant. Conclusions: Bronchoscopic lung volume reduction may be an effective and safe procedure for the treatment of severe COPD patients with emphysema based on existing studies.

MET Gene Copy Number Gain is an Independent Poor Prognostic Marker in Korean Stage I Lung Adenocarcinomas

Jin, Yan,Sun, Ping-Li,Kim, Hyojin,Seo, An Na,Jheon, Sanghoon,Lee, Choon-Taek,Chung, Jin-Haeng Springer - Society of Surgical Oncology 2014 Annals of Surgical Oncology Vol.21 No.2

Investigation of Key Circuit Constituents Affecting Drug Sequestration During Extracorporeal Membrane Oxygenation Treatment :

Park, Jiheum,Shin, Dong Ah,Lee, Saram,Cho, Young-Jae,Jheon, Sanghoon,Lee, Jung Chan,Kim, Hee Chan Published for the Society by J.B.Lippincott Co 2017 ASAIO journal Vol.63 No.3

<P>We quantified the influence of the elements of the extracorporeal oxygenation (ECMO) circuit on drug sequestration by focusing on the interactions between materials and drugs. Tubing of three different brands (Tygon/Maquet/Terumo) and oxygenators of two different brands (Maquet/Terumo) were used. Drugs included dexmedetomidine, meropenem, and heparin, which were dissolved in deionized water. Tubing was cut into approximately 7 cm sections and allowed drug solutions enclosed inside by clamping both ends. The oxygenator housing, gas membrane, and heat exchanger were dissected into approximately 1 g pieces and submerged into drug solutions. The experimental samples were then immersed in a water bath at 37 degrees C for 1, 6, 12, and 24 h. After 24 h, the dexmedetomidine concentration was significantly reduced in all three types of tubing (<30.1%), the oxygenator heat exchanger from Maquet Inc. (41.8%), and the gas exchanger from Terumo Inc. (8.6%), while no significant losses were found for meropenem and heparin compared with the control group. The heparin concentration within the Maquet gas exchanger, on the contrary, increased significantly compared with the control group at 1 and 12 h (p < 0.05). Our in vitro study reveals that material selection is a vital part of ECMO development.</P>

Prognostic stratification model for patients with stage I non-small cell lung cancer adenocarcinoma treated with surgical resection without adjuvant therapies using metabolic features measured on F-18 FDG PET and postoperative pathologic factors

Kang, Yeon-koo,Song, Yoo Sung,Cho, Sukki,Jheon, Sanghoon,Lee, Won Woo,Kim, Kwhanmien,Kim, Sang Eun Elsevier 2018 Lung cancer Vol.119 No.-

<P><B>Abstract</B></P> <P><B>Purpose</B></P> <P>In the management of non-small cell lung cancer (NSCLC), the prognostic stratification of stage I tumors without indication of adjuvant therapy, remains to be elucidated in order to better select patients who can benefit from additional therapies. We aimed to stratify the prognosis of patients with stage I NSCLC adenocarcinoma using clinicopathologic factors and F-18 FDG PET.</P> <P><B>Materials and methods</B></P> <P>We retrospectively enrolled 128 patients with stage I NSCLC without any high-risk factors, who underwent curative surgical resection without adjuvant therapies. Preoperative clinical and postoperative pathologic factors were evaluated by medical record review. Standardized uptake value corrected with lean body mass (SUL<SUB>max</SUB>) was measured on F-18 FDG PET. Among the factors, independent predictors for recurrence-free survival (RFS) were selected using univariate and stepwise multivariate survival analyses. A prognostic stratification model for RFS was designed using the selected factors.</P> <P><B>Results</B></P> <P>Tumors recurred in nineteen patients (14.8%). Among the investigated clinicopathologic and FDG PET factors, SUL<SUB>max</SUB> on PET and spread through air spaces (STAS) on pathologic review were determined to be independent prognostic factors for RFS. A prognostic model was designed using these two factors in the following manner: (1) Low-risk: SUL<SUB>max</SUB> ≤ 1.9 and no STAS, (2) intermediate-risk: neither low-risk nor high-risk, (3) high-risk: SUL<SUB>max></SUB>1.9 and observed STAS. This model exhibited significant predictive power for RFS.</P> <P><B>Conclusion</B></P> <P>We showed that FDG uptake and STAS are significant prognostic markers in stage I NSCLC adenocarcinoma treated with surgical resection without adjuvant therapies.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Stage I non-small cell lung cancer (NSCLC) patients recur not infrequently. </LI> <LI> There are no established guidelines that stratify the prognosis of stage I NSCLC. </LI> <LI> F-18 FDG PET/CT and spread through air spaces are independent prognostic factors. </LI> </UL> </P>

반복적인 부비동염으로 내원한 Good 증후군 1예

송경호,김계형,김충종,박경운,전상훈,김홍빈,김남중,오명돈,최강원 대한감염학회 2007 감염과 화학요법 Vol.39 No.5

Good 증후군은 흉선종과 면역결핍이 동반되는 질환으로, B세포의 감소로 인한 범저감마글로불린혈증과 CD4^(+)T세포의 감소로 인한 세포성 면역저하가 동시에 나타나는 것을 특징으로 한다. 본 증례는 18개월 전부터 발생한 반복적인 부비동염을 주소로 내원한 43세 남자 환자로, 내원 1년 전 흉선종절제수술을 시행받았으며, 면역학적 검사상 범저감마글로불린혈증과 T세포의 감소 등의 이상 소견이 발견되었다. 정주 면역글로불린과 경험적 항생제(amoxicillin/clavulanic acid) 사용 후 부비동염이 호전되었으며, 예방접종과 정기적인 면역글로불린 투여 후 특이 합병증없이 경과 관찰 중이다. Good's syndrome is the association of thymoma with immunodeficiency, characterized by hypogammaglobulinemia, B-cell lymphopenia and variably defects in cellular immunity with CD4^(+) T-cell lymphopenia and an inverted CD4^(+):CD8^(+) T-cell ratio. We report a 43-year-old male patient who presented with a 18-month history of productive cough and postnasal drip. One year ago, he underwent the operation for resection of a thymoma. Despite of appropriate management, sinusitis relapsed multiple times. He was found to have hypogammaglobulinemia with nearly absent B cells(4/μL). The CD4^(+) T-cell count was 554/μL with an inverted CD4^(+):CD8^(+) T-cell ratio of 0.6. His symptoms and signs improved with antibiotic treatment and monthly administration of intravenous immunoglobulin (IVIG, 400 mg/kg).

ROS1 gene rearrangement and copy number gain in non-small cell lung cancer.

Jin, Yan,Sun, Ping-Li,Kim, Hyojin,Park, Eunhyang,Shim, Hyo Sup,Jheon, Sanghoon,Kim, Kwhanmien,Lee, Choon-Taek,Chung, Jin-Haeng Springer International 2015 Virchows Archiv Vol.466 No.1

<P>ROS1 has attracted much attention as a possible oncogenic driver and ROS1-rearranged tumors show sensitivity to most ALK inhibitors. We aimed to clarify the prevalence of ROS1 gene rearrangement and investigate the clinical implications of ROS1 gene copy number gain (CNG) in non-small cell lung cancer (NSCLC) patients. We carried out fluorescent in situ hybridization with ROS1 and centromere enumeration 6 probes and immunohistochemistry for ROS1 protein expression. ROS1 rearrangement was detected in 3 of 375 samples (0.8 %); all of whom were female, never-smokers, and harbored an adenocarcinoma component. ROS1 gene CNG was found in 18 cases (4.8 %). ROS1 gene CNG was significantly associated with shorter disease-free survival (DFS, 12 vs. 58 months; p = 0.003) and shorter overall survival (OS, 40 vs. 67 months; p <0.001) than the group without CNG. Multivariate analysis confirmed that ROS1 gene CNG was significantly associated with poorer DFS (hazard ratio [HR]=2.16, 95 % confidence interval [CI] = 1.22-3.81, p = 0.008), and OS ([HR] = 2.53, 95 % [CI] = 1.31-4.89, p = 0.006). ROS1 protein overexpression was observed in 5.0 % (18 out of 357), of which 2 cases harbored ROS1 gene rearrangement. There was no statistically significant correlation between ROS1 gene CNG and protein overexpression. This study demonstrated ROS1 gene rearrangement was detected in 0.8 % of surgically resected NSCLC; and ROS1 gene CNG is an independent poor prognostic factor. This survival analyses may contribute to future studies on the utility of ROS1-targeted therapy for patients.</P>

High concordance of<i>EGFR</i>mutation status between histologic and corresponding cytologic specimens of lung adenocarcinomas : Cytologic Assessment of<i>EGFR</i>Mutation

Sun, Ping-Li,Jin, Yan,Kim, Hyojin,Lee, Choon-Taek,Jheon, Sanghoon,Chung, Jin-Haeng Wiley (John WileySons) 2013 Cancer cytopathology Vol.121 No.6

<P>Activating mutations in the epidermal growth factor receptor (EGFR) in non-small cell lung carcinoma (NSCLC) are associated significantly with responsiveness to EGFR tyrosine kinase inhibitors. The objective of this study was to investigate the suitability of cytologic specimens for assessing EGFR mutations in lung adenocarcinomas.</P>