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Novel Methylation Biomarker for Non-invasive Diagnostics in Lung Cancer
오태정,( Chang Hun Lee2 ),( Min Ki Lee ),( Yeul Hong Kim ),( Sang Yull Lee ),( Hyo Sung Jeon ),( Shin Yup Lee ),( Seung Soo Yoo ),( Jae Yong Park ),( Sung Whan An ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-
To identify aberrantly hypermethylated DNA in lung cancer cells we established a genome-wide analysis for hypermethylation sites, namely Methyl DNA Isolation and Amplification (MeDIA) coupled-CpG microarray analysis. In the comprehensive methyaltion profiling analysis between human lung cancer, A549 cells and normal NHBE cells, we observed that several clusters of genes show a significant level of aberrancy in CpG island methylation pattern in cancer cells compared to normal cells. We further identified PCDHGA12 gene as a new marker of non-invasive diagnostics for lung cancer based on followings. 1) Transcription of PCDHGA12 gene is reactivated after treatment of A549 cells with demethylating agent. 2) Bisulfide clonal-sequencing reveals that CpG island of PCDHGA12 shows a distinctive differential methylation between two cell lines. 3) Pyrosequencing-based quantitative methylation assay for such region in tumor and non-tumorous tissues from lung cancer patients shows aberrant hypermethylation in 37 (92%) of the 40 tumor tissues. In clinical validation by pyrosequencingin induced-sputum of lung cancer patients (n=87) and healthy controls (n=51), we observed aberrant hypermethylation incident at significantly elevated level in samples derived from lung cancer patients. According to the optimal threshold calculated by ROC curve analysis, sensitivity and specificity of PCDHGA12 was 86.2% and 82.4%, respectively. PCDHGA12 methylation status could be a potential methylation biomarker alone or combined with others for the screen and the detection of relapse of lung cancer.
Lee, Sang-Yup,Lee, Pyung-Cheon,Hong, Soon-Ho,Chang, Ho-Nam The Korean Society for Biotechnology and Bioengine 2002 Biotechnology and Bioprocess Engineering Vol.7 No.2
A pckA gene encoding phosphoenolpyruvate carboxykinase (PEPCK) was cloned and sequenced from the succinic acid producing bacterium Mannheimia succiniciproducens MBEL55E. The gene encoded a 538 residue polypeptide with a calculated molecular mass of 58.8 kDa and a calculated pI of 5.03. The deduced amino acid sequence of the M. succiniciprodutens MBEL55E PEPCK was similar to those of all known ATP-dependent PEPCKS.
Systems-Level Analysis of Genome-Scale In Silico Metabolic Models Using MetaFluxNet
Lee, Sang-Yup,Woo, Han-Min,Lee, Dong-Yup,Choi, Hyun-Seok,Kim, Tae-Yong,Yun, Hong-Seok The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.5
The systems-level analysis of microbes with myriad of heterologous data generated by omics technologies has been applied to improve our understanding of cellular function and physiology and consequently to enhance production of various bioproducts. At the heart of this revolution resides in silico genome-scale metabolic model, In order to fully exploit the power of genome-scale model, a systematic approach employing user-friendly software is required. Metabolic flux analysis of genome-scale metabolic network is becoming widely employed to quantify the flux distribution and validate model-driven hypotheses. Here we describe the development of an upgraded MetaFluxNet which allows (1) construction of metabolic models connected to metabolic databases, (2) calculation of fluxes by metabolic flux analysis, (3) comparative flux analysis with flux-profile visualization, (4) the use of metabolic flux analysis markup language to enable models to be exchanged efficiently, and (5) the exporting of data from constraints-based flux analysis into various formats. MetaFluxNet also allows cellular physiology to be predicted and strategies for strain improvement to be developed from genome-based information on flux distributions. This integrated software environment promises to enhance our understanding on metabolic network at a whole organism level and to establish novel strategies for improving the properties of organisms for various biotechnological applications.
Lee, Sang-Yup,Park, Jong-Pil,Lee, Seok-Jae,Park, Tae-Jung,Lee, Kyung-Bok,Park, Insung S.,Kim, Min-Gon,Chung, Bong-Hyun The Korean Society for Biotechnology and Bioengine 2004 Biotechnology and Bioprocess Engineering Vol.9 No.2
In this study, a simple procedure is described for patterning biotin on a glass substrate and then selectively immobilizing proteins of interest onto the biotin-patterned surface. Microcontact printing (CP) was used to generate the micropattern of biotin and to demonstrate the selective immobilization of proteins by using enhanced green fluorescent protein (EGFP) as a model protein, of which the C-terminus was fused to a core streptavidin (cSA) gene of Streptomyces avidinii. Confocal fluorescence microscopy was used to visualize the pattern of the immobilized protein (EGFP-cSA), and surface plasmon resonance was used to characterize biological activity of the immobilized EGFP-cSA. The results suggest that this strategy, which consists of a combination of $\mu$CP and cSA-fused proteins. is an effective way for fabricating biologically active substrates that are suitable for a wide variety of applications. one such being the use in protein-protein assays.
Lee,Byung Chun,Kim, Sung JIn,Hur, Moon Hyu,Lee, Eun Yup,Ahn, MoonKyu 慶星大學校 1994 論文集 Vol.15 No.4
Sodium dodecyl sulfate, zephiramine, triton X-100을 사용하여 각 물질의 CMC를 결정하고, 온도, 전해질이 미셀 형성에 미치는 영향과 그의 polarity, viscosity에 관한 변화를 소수성 형광 probe인 pyrene을 사용하여 관찰하였다. 25℃에서 측정된 CMC의 값은 SDS; 7.80mM, zephiramine; 1.65mM, triton X-100; 0.28mM이었으며 온도가 증가됨에 따라 CMC의 값은 점차 감소되었다. 염을 첨가시 더욱 낮은 온도에서 미셀을 형성할 수 있었으며, 미셀의 점도는 약간 높은 치를 나타내었고 극성은 낮았다. 혼합 미셀의 경우 계면 활성제의 몰분율이 클수록 점도는 낮아지고 반면에 극성은 증가하였다.
Lee, Hyesung,Lee, Sang-Yup Elsevier 2018 JOURNAL- TAIWAN INSTITUTE OF CHEMICAL ENGINEERS Vol.92 No.-
<P><B>Abstract</B></P> <P>Modern light wearable electrical devices require flexible, conductive materials for the development of portable and/or wearable devices. In this study, a flexible carbon nanotube (CNT) electrode is prepared, and the conductive CNTs are deposited on a flexible polydimethylsiloxane (PDMS) substrate using a combination of inkjet printing and transfer printing methods. Aqueous CNT ink is printed on a commercial overhead projector film. The subsequent deposition of a thin PDMS layer and peeling the layer off resulted in the production of a flexible PDMS film with conductive CNT patterns. The thickness of the prepared electrode increased by ∼1.2 µm after every 10 prints, and the sheet resistance decreased rapidly from 14.7 MΩ/sq to 913 kΩ/sq after 20 and 50 prints, respectively. The construction of a simple foldable electrical circuit and its application to the electrochemical sensing of dopamine using flexible electrodes are demonstrated. These flexible electrodes can be easily fabricated while displaying designer functionality.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A flexible carbon nanotube electrode was prepared by inkjet printing and transfer printing methods. </LI> <LI> The flexible electrode was utilized for detection of dopamine. </LI> <LI> Thin ink receptive layer increased resistance and afforded selectivity to dopamine. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>A flexible carbon nanotube (CNT) electrode is prepared using a combination of inkjet printing and transfer printing methods. The flexible electrode is applied for the construction of a foldable electrical circuit and for the electrochemical sensing of dopamine.</P> <P>[DISPLAY OMISSION]</P>