Carnosine Promotes Exhaustive Swimming Capacity in Mice

Jong-Soo Kim, Ki Nam Lee, Jin-Joo Hue, Jun-Hyeong Kim, Bong Soo Kang, Sang Yoon Nam, Young Won Yun, Jae-Hwang Jeong2, Beom Jun Lee 충북대학교 동물의학연구소 2011 Journal of Biomedical and Translational Research Vol.12 No.1

To investigate the effect of carnosine on exhaustive exercise, swimming tests were conducted weekly with loads corresponding to 5% of body weight attached to the tails of mice, and the swimming time to exhaustion was measured. Eighty male ICR mice were divided into four groups, to which carnosine was administered at doses of 0 (control), 10, 50, and 250 mg/kg/day, respectively, for a period of four weeks. At the end of swimming exercise challenges, serum biochemistry, oxidative stress enzyme activity, and antioxidant enzyme activity in tissues were determined. Treatment with 250 mg/kg carnosine resulted in a significant increase in swimming times to exhaustion, compared to the control group in the first (P<0.01) and third week (P<0.05). Significantly lower serum lactate levels were observed after the swimming exercise in the carnosine-treated groups (10 and 250 mg/kg), compared with the control (P<0.01). Malondialdehyde levels in the liver (10 and 50 mg/kg carnosine treated groups) and skeletal muscle (250 mg/kg carnosine treated group) were significantly lower, compared with the control (P<0.05). Significantly lower protein carbonyl levels in skeletal muscle were observed in the 50 and 250 mg/kg carnosine treated groups, compared with the control (P<0.01). Superoxide dismutase and glutathione peroxidase activities in skeletal muscle did not differ significantly among the groups. These results indicate that carnosine may improve swimming exercise capacity by attenuating production of lactate and reducing oxidative stress in mice.

Interaction of <i>Veratrum nigrum</i> with <i>Panax ginseng</i> against Obesity: A Sang-ban Relationship

Park, Jinbong,Jeon, Yong-Deok,Kim, Hye-Lin,Lim, Hara,Jung, Yunu,Youn, Dong-Hyun,Jeong, Mi-Young,Kim, Hyun-Ju,Kim, Sung-Hoon,Kim, Su-Jin,Hong, Seung-Heon,Um, Jae-Young Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-

<P>Obesity has become a major health threat in developed countries. However, current medications for obesity are limited because of their adverse effects. Interest in natural products for the treatment of obesity is thus rapidly growing. Korean Medicine (KM) is characterized by the wide use of herbal formulas. However, the combination rule of herbal formulas in KM lacks experimental evidence. According to <I>Shennong's Classic of Materia Medica</I>, the earliest book of herbal medicine, <I>Veratrum nigrum</I> (VN) has antagonistic features against <I>Panax ginseng</I> (PG), and the PG-VN pair is strictly forbidden. In this study, we have shown the effects of PG, VN, and their combination on obesity in high-fat (HF) diet-induced obese mice and in 3T3-L1 cells. PG, VN, and PG-VN combination significantly reduced weight gain and the fat pad weight in HF diet-induced obese mice. They also significantly decreased lipid accumulation and the expressions of two major adipogenesis factors, PPAR<I><I>γ</I></I> and C/EBP<I><I>α</I></I>, in 3T3-L1 cells. In addition, the PG-VN combination had synergistic effects compared with the mixture of extracts of PG and VN on inhibition of PPAR<I><I>γ</I></I> and C/EBP<I><I>α</I></I> expressions at lower doses. These results indicate a new potential anti-obese pharmacotherapy and also provide scientific evidence supporting the usage of herbal combinations instead of mixtures in KM.</P>

[PA-0036] Genetic diversity of plastid genome and 45S rDNA sequences in genus Ligularia (Asteraceae)

Seon Heui Jeong(Seon Heui Jeong),Young Sang Park(Young Sang Park),Jeong Jin Choi(Jeong Jin Choi),Tae-Jin Yang(Tae-Jin Yang) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Enhanced development of porcine embryos cloned from bone marrow mesenchymal stem cells

Jin, Hai-feng,Kumar, B Mohana,Kim, Jung-Gon,Song, Hye-Jin,Jeong, Yeon-Ji,Cho, Seong-Keun,Balasubramanian, Sivasankaran,Choe, Sang-Yong,Rho, Gyu-Jin UPV/EHU Press 2007 The international journal of developmental biology Vol.51 No.1

<P>In the present study, we have characterized an isolated population of porcine bone marrow mesenchymal stem cells (MSCs) for multilineage commitment and compared the developmental potential of cloned embryos with porcine MSCs and fetal fibroblasts (FFs). MSCs exhibited robust alkaline phosphatase activity and later transformed into mineralized nodules following osteoinduction. Furthermore, MSCs underwent adipogenic and chondrogenic differentiation by producing lipid droplets and proteoglycans, respectively. Primary cultures of FFs from a female fetus at ~30 day of gestation were established. Donor cells at 3-4 passage were employed for nuclear transfer (NT). Cell cycle analysis showed that the majority of MSCs in confluence were in the G0/G1 stage. Cumulus-oocyte complexes were matured and fertilized in vitro (IVF) as control. The cleavage rate was significantly (P<0.05) higher in IVF than in NT embryos with MSCs and FFs (84.54.6% vs. 52.25.4% and 50.85.2%, respectively). However, blastocyst rates in IVF and NT embryos derived from MSCs (20.62.5% and 18.43.0%) did not differ, but were significantly (P<0.05) higher than NT derived from FFs (9.52.1%). Total cell number and the ratio of ICM to total cells among blastocysts cloned from MSCs (34.45.2 and 0.380.08, respectively) were significantly (P<0.05) higher than those from FFs (22.65.5 and 0.180.12, respectively). Proportions of TUNEL positive cells in NT embryos from FFs (7.31.8%) were significantly (P<0.05) higher than in MSCs (4.61.3%) and IVF (2.50.9%). The results clearly demonstrate that multipotent bone marrow MSCs have a greater potential as donor cells than FFs in achieving enhanced production of cloned porcine embryos.</P>

Effect of Vital Tooth Bleaching Agent on Dentin Bonding : 생활치 미백제가 상아질 접착에 미치는 영향

Jeong, Na-Young,Jin, Myoung-Uk,Kim, Young-kyung,Kim, Sung Kyo 大韓齒科保存學會 2006 Restorative Dentistry & Endodontics Vol.31 No.2

생활치 미백제와 알코올 전처리 과정이 상아질 접착에 미치는 영향을 평가하고자 발거치 55개의 치관부 협측 상아질면에서 Opalescence^(®) (Ultradent사, 미국)로 미백처리 후 즉시 One-step^(®) 상아질 접착제(Bisco사, 미국)를 전처치한 Z-250^(®) 복합레진(3M-ESPE사, 미국) 접착군, 미백처리 2주후 레진 접착군, 미백처리 후 70% 에탄올 처리군, 그리고 미백처리하지 않은 대조군으로 나누어 실험한 다음 전단접착강도를 측정하고 95% 유의수준에서 일원변량분석법으로 통계분석 하였다. 생활치 미백제가 상아질 접착력을 현저히 감소시켰다. 미백처리 2주 후 레진 접착군은 미백처리하지 않은 대조군과 유의한 차이가 나타나지 않았으며 70% 에탄올 처리군은 미백처리하지 않은 대조군보다는 접착력이 낮았으나, 미백처리 2주 후 레진 접착군과는 차이가 없고 즉시 접착군보다는 높은 접착강도를 나타내었다. 따라서 임상에서 치아미백 후 즉시 레진수복을 할 경우, 에탄올로 전처리하면 상아질에서 레진 접착력을 회복할 수 있을 것으로 사료된다. To evaluate the effect of vital tooth bleaching agent and alcohol pretreatment on dentin bonding, flat dentin windows were produced on the buccal side of the crowns of fifty-five extracted, human premolars. A bleaching gel, Opalescence^(®) with 10% of carbamide peroxide (Ultradent Product, USA) was daily applied on the teeth of three experimental groups for six hours for 10 consecutive days, while teeth of a control group were not bleached. After 6 hours of bleaching gel applicatiion, the specimens were washed and stored in saline until the next day application. After application of One-step^(®) dentin bonding agent (Bisco, USA), Z-250^(®) resin (3M-ESPE, USA) was bonded to dentin with a mount jig. Shear bond strength was measured with an Instron machine (Type 4202, Instron Corp., USA) after 24 hours. The results were analyzed using one-way ANOVA and Duncan's multiple range test at p < 0.05. Immediate bonding group showed significantly lower bond strength than un-bleached control group (p < 0.05). Ethanol-treated group showed significantly higher bond strength compared to immediate bonding group (p < 0.05). However, the bond strength of the ethanol treatment group was lower than tha of the un-bleached control group (p < 0.05). There were no significant difference in shear bond strength between the 2-week delayed bonding group and the ethanol-treated group (p > 0.05) and between delayed bonding group and un-bleached control troup (p > 0.05). In the condition of the present study, it seems that alcohol pretreatment after bleaching procedure can reduce the adverse effect of vital bleaching agent on dentin bonding.

The effects of different night-time temperatures and cultivation durations on the polyphenolic contents of lettuce: Application of principal component analysis

Jeong, Sung Woo,Kim, Gon-Sup,Lee, Won Sup,Kim, Yun-Hi,Kang, Nam Jun,Jin, Jong Sung,Lee, Gye Min,Kim, Soo Taek,Abd El-Aty, A.M.,Shim, Jae-Han,Shin, Sung Chul Elsevier 2015 Journal of advanced research Vol.6 No.3

<P>The present study was conducted to characterize the polyphenolic contents of lettuce leaves grown under different night-time temperatures (4, 12, and 20 °C) and cultivation durations (5, 15, and 20 days) using high performance liquid chromatography-tandem mass spectrometry (LC/MS/MS). The assay method was validated based on specificity, linearity, accuracy, precision, and the performance limit. The total polyphenolic contents were highest (2462.6 mg/kg) after transplantation at a night temperature of 20 °C on day 20 and lowest (1132.7 mg/kg) at the same temperature on day 5. Quantification and principal component analysis showed that the relative contents of quercetin and kaempferol were markedly higher during the early stage of cultivation (day 5) than those of day 15 and 20, and that night-time temperatures of 12 and 20 °C on day 20 were favorable for producing polyphenol-rich lettuce containing caffeic acid. In conclusion, a synergistic effect between high night-time temperatures (12 and 20 °C) and cultivation duration (20 days) produced lettuce rich in polyphenols compared to that at low temperature (4 °C).</P>

Multicenter analysis of treatment outcomes in adult patients with lymphoblastic lymphoma who received hyper-CVAD induction followed by hematopoietic stem cell transplantation.

Jeong, Seong Hyun,Moon, Joon Ho,Kim, Jin Seok,Yang, Deok-Hwan,Park, Yong,Cho, Seok Goo,Kwak, Jae-Yong,Eom, Hyeon Seok,Won, Jong Ho,Hong, Jun Shik,Oh, Sung Yong,Lee, Ho Sup,Kim, Seok Jin Springer International 2015 Annals of hematology Vol.94 No.4

<P>The hyperfractionated cyclophosphamide, vincristine, doxorubicin, and dexamethasone (hyper-CVAD) regimen has been widely used for lymphoblastic lymphoma (LBL) as a primary treatment. However, there is few data about its treatment outcome in Asian patients. Thus, we conducted this study to evaluate the efficacy of hyper-CVAD induction and stem cell transplantation (SCT) consolidation in LBL patients. The treatment responses of 49 patients treated with the hyper-CVAD regimen were retrospectively analyzed in 13 institutions. Given 24 patients who responded to hyper-CVAD underwent consolidation treatment with SCT, overall survival (OS) and progression-free survival (PFS) of patients who received SCT were compared with patients who did not. The overall response rate was 79 %: 73 % (36/49) complete responses, 6 % (3/49) partial responses, and 4 % (2/49) induction deaths. The major limitation for the delivery of the planned hyper-CVAD cycles was hematological toxicity. Among 39 responders, 24 patients underwent autologous (n?=?16) and allogeneic SCT (n?=?8) consolidation. Their 3-year OS and PFS rates were 76 and 78 %, respectively, and there was no difference in survival outcomes between autologous and allogeneic SCT. However, 15 patients without SCT consolidation showed poorer PFS even though they all achieved complete response. Thus, only seven patients maintained their response at the time of analysis. In conclusion, the hyper-CVAD regimen is effective for remission induction in LBL, and SCT consolidation after hyper-CVAD induction produced better clinical outcomes than did continuation of hyper-CVAD.</P>

Nitrogen Dynamics in Soil Amended with Different Rate of Nitrogen Fertilizer

Sung Un Kim,Eun-Jung Choi,Hyun-Cheol Jeong,Jong-Sik Lee,Hyun Ho Lee,Hye Jin Park,Chang Oh Hong 한국토양비료학회 2017 한국토양비료학회지 Vol.50 No.6

Excessive application of nitrogen (N) fertilizer to support switchgrass growth for bioenergy production may cause adverse environmental effects. The objective of this study was to determine optimum N application rate to increase biomass yield of switchgrass and to reduce adverse environmental effects related to N. Switchgrass was planted in May 2008 and biomass yield, N uses of switchgrass, nitrate (NO₃) leaching, and nitrous oxide (N₂O) emission were evaluated from 2010 through 2011. Total N removal significantly increased with N rate despite the fact that yield did not increased with above 56 kg N ha<SUP>-1</SUP> of N rate. Apparent nitrogen recoveries were 4.81 and 5.48% at 56 and 112 kg N ha<SUP>-1</SUP> of N rate, respectively. Nitrogen use efficiency decreased into half with increasing N rate from 56 to 112 kg N ha<SUP>-1</SUP>. Nitrate leaching and N₂O emission were related to N use of switchgrass. There was no significant difference of cumulative NO₃ leaching between 0 and 56 kg N ha<SUP>-1</SUP> but, it significantly increased at 112 kg N ha<SUP>-1</SUP>. There was no significant difference of cumulative N₂O emission among N rates in crest, but it significantly increased at 112 kg N ha<SUP>-1</SUP> in toe. Excessive N application rate (above 56 kg N ha<SUP>-1</SUP>) beyond plant requirement could accelerate NO₃ leaching and N₂O emission in switchgrass field. Overall, 56 kg N ha<SUP>-1</SUP> might be optimum N application rate in reducing economic waste on N fertilizer and adverse environmental impacts.

Inhibition of a Neutral Form of Sphingomyelinase by Alkylthioureido-1,3-propandiols, KY353X Series

( Sang Mi Jung ),( Eui Man Jeong ),( Dong Hwawn Jo ),( Mi Reyoung Chin ),( Hyung Jin Jun ),( Yong Hyun Kim ),( Hyung Jun Jeon ),( Dong Hun Lee ),( Mi Ja Park ),( Mi Jung Oh ),( Chul Bu Yim ),( Dae Kyo 한국응용약물학회 2003 Biomolecules & Therapeutics(구 응용약물학회지) Vol.11 No.3

Clinical features and prognostic factors in papillary thyroid microcarcinoma depends on age

Jin-Kyu Cho,Ju-Yeon Kim,Chi-Young Jeong,Eun-Jung Jung,Soon-Tae Park,Sang-Ho Jeong,Young-Tae Ju,Young-Joon Lee,Soon-Chan Hong,Woo-Song Ha,Sang-Kyung Choi 대한외과학회 2012 Annals of Surgical Treatment and Research(ASRT) Vol.82 No.5

Purpose: Clinical outcomes of papillary thyroid microcarcinoma (PTMC) vary. In general, age at diagnosis is an independent prognostic factor in conventional papillary thyroid carcinoma. However, it is unclear in patients of PTMC. The purpose of this study was to identify clinicopathologic features and prognostic factors of PTMC according to patients’ age. Methods: Five hundred twenty-seven patients who received thyroid surgery and diagnosed as having PTC between January 2001 and December 2009 were included. The clinical data were retrospectively analyzed. Results: We divided the patients into two groups; group Ⅰ who were younger than 45 years, and group Ⅱ who were 45 years old or older. The mean tumor size and incidences of neck lymph nodes involvement of group I was larger than group Ⅱ. In group Ⅱ, however, there were more patients who had multiple cancer foci and were body mass index ≥ 25 ㎏/㎡. The overall incidence of recurrent disease was 3.2%. The incidence of recurrence was higher in group Ⅱ (2.0% vs. 4.0%), without a statistical difference. In multivariate analysis, the significant risk factors of recurrence were male gender and multifocality in group Ⅰ, and lymph node metastasis and multifocality in group Ⅱ. In particular, the male gender and multifocality showed the highest odds ratio (OR) on each group (OR, 4.721 and 6.177). Conclusion: The patients with PTMCs had different clinical features and prognostic factors according to age. Hence, clinicians should consider a different strategy for therapy and plan for follow-up according to age.