Studies on the Effects of Biomedicinal Agents on Serum Concentration of Ca2+ , P and ALP Activity in Osteoporosis-Induced Rats

Sang-keun Kim,Myung-hun Lee,Man-hee Rhee 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

on the Effects of Biomedicinal Agents on Serum Concentration of Ca2+, P andALP Activity in Osteoporosis-Induced RatsSang-keun Kim, Myung-hun Lee1 and Man-hee Rhee2College of Veterinary Medicine, Chungnam National University, 220 Gungdong, Yusong-Gu, Daejeon 305-764, Korea1National Veterinary Research and Quarantine Service, Anyang 430-824, Korea2College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, KoreaReceived April 3, 2003 / Accepted July 9, 2003J. Vet. Sci. (2003), 4(2), 151-154JOURNAL OFVeterinaryScience*Corresponding author: Sang-keun Kim Dept. of Vet. Med., Chungnam National University, Daejeon 305764, Korea Tel: +82-42-821-6754, Fax: +82-42-821-6754 E.-mail: kskkim@cnu.ac.kr

유교적 본원 가치와 로컬리티의 왜곡 ―근대성 성취 과정의 집중 문제를 중심으로―

이명수 ( Myung Su Rhee ),이상봉 ( Sang Bong Lee ) 한국철학사연구회 2012 한국 철학논집 Vol.0 No.33

한국의 근대화는 우리에게 괄목할만한 성취를 안겨 주었지만, 그 이면에 중앙 편중, 이분법적 사물 접근, 획일화, 주변화, 타자화의 “결여의 공간”을 낳았다. 한편 유교는 근대화 과정에 중요 이념적 요소여서 그 성취에 정신적 지주 역할을 하였을 뿐만 아니라, 결여 현상에 일정 부분 잘못 이용되기도 하였다. 이 같은 문제의식에서, 이 논문은 두 층위의 유교, 지배 이념적 정치 유학과 본원 유교에 주목하여 그 성찰을 요청한다. 또한 “실학”으로서 유교가 결코 바람직하지 않은 현대 한국의 중심화 해소에 어떤 역할을 할 수 있을지도 모색해 본다. 그렇게 함으로써 오늘을 사는 한국적 공간에 내재하는 오도된 가치, 국가 아이덴티티, 중앙 집중으로부터 비롯된 본원 가치-문명적 가치(유교)뿐만 아니라 사물이나 장소 가치-의 왜곡된 인식에 대하여 접근한다. 아울러 이 논문은 우리 사회에 만연된 사물이나 지역이 지닌 가치 발현의 문제, 근대화 과정에 만들어진 집중, 쏠림 현상을 벗어나기 위한, “탈중심”의 대안은 없는지에 관한 검토한다. Korea has brought the remarkable modernization which is led by the government, but on the other hand it brought about many negative results such as nation-centralism, the logical dichotomy, standardization, marginalization, lack of space, and so on. In the process, Confucianism not only have played the part of the mental leader in achievement but also misused in formation of “lack of space” as a negative results partly, From these critical mind, this thesis asks for the reflection of Confucianism in the focus of two level that are Political Confucianism and Authentic Confucianism. At the same time we try to find the role of Confucianism as a practical science in solving the negative results of modernization. As a result, we describe the cause of the distortion of Authentic Confucianism inherent in Korean contemporary society. Additionally, this thesis analyzes the possibility of Authentic Confucianism as an alternative value to nation-centralism which has brought about political inequality and social polarization.

이온교환수지를 이용한 새로운 오메프라졸 복합체 개발

이계주,이기명,김은영,이창현,황성주 충남대학교 약학대학 의약품개발연구소 1994 藥學論文集 Vol.10 No.-

Omeprazole(OMZ)-cholestyramine(CHL) and various OMZ-Dowex resin complexes were prepared by reaction between OMZ and activated resins in 0.1N NaOH solution. And their physical properties were tested by means of infrared(IR), differential scaning caloimeter(I)SC), X-ray diffraction. Chemical stability of OMZ-CHL was increased markedly compared with OMZ and the decomposition of OMZ-CHI, followed the pseudo first-order kinetics and the rate constants were 2.743×10 ^4/day at 20℃, 7.83×10 ^3day under 80% RH and 1.68×10 ^2day under UV radiation, respectively. On the other hand, the rate constants of OMZ were 2.996×10 ^4day at 20℃, 1.17×10 ^4day under 85% RH, and 4.07×10^2 day under UV radiation, respectively. The rates of dissolution of OMZ-CHL bulk and OMZ-CHI, tablet were 100% and more than 85% in 15 minutes, respectively, which were increased than OMZ base and OMZ-tablet. In the acute toxicological test, the value of oral LD_50(mouse) was 4.608 g/㎏. OMZ-CHL was pelletized using lactose, polyethyle neglycol(PEG), D-sorbitol, Avicel PH 101, sodium laurylsulfate and polyvinylpyrrolidone(PVP) K-30, and enteric coated with HPMCP, Myvacet, acetone, ethanol and cetanol, of which dissolution rate was found to be more than 85% in 10 minutes. From the above results, it was found that OMZ-OML, is a useful means for development of new oral dosage forms of OMZ.

Helicobacter pylori 편모 유전자의 클로닝 및 염기서열 분석

이광호,이우곤,조명제,도영미,백승철,강경희,박필성,이상룡 경상대학교 유전공학연구소 1993 遺傳工學硏究所報 Vol.12 No.-

A λgt11 expression libary of H. pylori DNA in E. coli Y1090 was screened with flagellin-specific rabbit antiserum for molecular cloning of the flagellin gene of H. pylori. A positive clone, λHPF4, was obtained and the recombinant antigen expressed from λHPF4 was a fusion protein with the molecular weight of 168kd. Sequence analysis of antigen-encoding DNA showed that an open reading frame composed of 1,536 nucleotides encodes a polypepride with a oredicted molecular size of 54kd. This open reading frame did not show the homology with flaA gene encoding 56kd protein of H. pylori and was confirmed as a unique sequence through homoligy searching. Therefore, the cloned antigen is supposed to be the carboxy-terminal region of the other flagellin protein of H. pylori, flaB, with the molecular weight of 58kd.

오메프라졸과 Hydroxypropyl-β-cyclodextrin의 포접화합물 형성과 특성

이계주,황성주,이기명 충남대학교 약학대학 의약품개발연구소 1993 藥學論文集 Vol.9 No.-

Inclusion complex of omeprazole(OMZ) with hydroxypropyl-β-cyclodextrin(HP-β-CD) was prepared by freeze-drying method. The complexation was confirmed by means of UV, CD, IR, DSC, XRD and ^1H-NMR spectra. The benzimidazole moiety of OMZ might be found to be included into the cavity of HP-β-CD and the inclusion complex appeared to be A_L type. The stoichiometric ratio of OMZ:HP-β-CD was found to be 1:1, and the stability constants of the inclusion complex by solubility and UV method were about 34 and 45 M^-1, respectively. The dissolution of OMZ was significantly enhanced from powder and, yablet of OMZ-HP-β-CD complex when compared to those of OMZ alone, and oil-to-water partition coefficient of OMZ-HP-β-CD complex was significantly higher than that of OMZ alone. Therefore, it was expected that the bioavailability of OMZ could be improved markedly by inclusion complexation of OMZ with HP-β-CD.

한 대학 신입생의 우울수준과 관련변인에 관한 연구

이명학,남해성,손석준,이정애,김병우 대한보건협회 1994 대한보건연구 Vol.20 No.2

1994년도 한 대학 1년생을 대상으로 8개항목의 생활만족지수(LSI-W) 그리고 20개항목의 우울지수(CES-D) 및 관련변인들을 조사하여 각각의 점수와 우울증의 유병률을 계산하고 서로간의 관련성을 조사하여 다음과 같은 결과를 얻었다. 1. 생활만족지수의 평균점수는 9.98이었고 비흡연군, 비음주군, 운동을 하는 군에서 평균점수가 유의하게 높았다(p<0.01). 2. 우울지수의 평균점수는 17.2였고 여학생에서 남학생보다 유의하게 높았고, 우울증의 유병률은 19.8%이었고 여학생에서 남학생보다 유의하게 높았다(p<0.01). 3. 우울증세 고위험군과 저위험군에 있어서 건강에 대한 본인의 생각과 본인이 인지하는 체중에서 차이가 있었으며, 생활만족지수, 가족수, 체중, 신장에서 유의한 차이를 보였다(p<0.05). 4. 우울지수와 생활만족지수, 연령, 종교활동기간, 신장, 체중등과는 음의 상관을 나타내고(p<0.01), 가족수, 수면시간, 경제수준과는 양의 상관을 보였다(p<0.01). 5. CES-D에 영향을 미치는 변수를 알아보기 위한 단계적 로지스틱 회귀분석에서 생활만족도, 성별, 건강에 대한 본인의 생각, 본인이 인지하는 체중, 흡연, 음주가 CES-D에 의한 우울증 발현에 있어서의 유의한 요인으로 나타났다(p<0.05). The questionnaire survey using the Center for Epidemiological Studies-Depression Scale(CES-D) and 8-items Life Satisfaction Index Well-being(LSI-W) and other variables was conducted to determine the prevalence of depression and to identify the correlation among CES-D and related factors. Study population was the whole freshman group newly enrolled in a university. The results were as follows; 1. Mean score of LSI-W was 9.98 and that score was higher in non-smokers, non-drinkers and exercise group (p<0.01). 2. Mean score of CES-D was 17.2 and the prevalence of depression was 19.8%. And those were significantly higher in female than in male(p<0.01). 3. Perceived health status, perceived weight, LSI-W, family size, weight and height were significant variables between high and low risk group of depression(p<0.05). 4. CES-D score was negatively correlated with LSI-W, age, duration of religious life, height, weight and po-sitively correlated with family size, sleeping time and economic status(p<0.01). 5. In the regression analysis significant predictors of the depression were sex, preceived health status, weight, smoking and drinking(p<0.05).

Alginate Bead를 이용한 고분자 약물의 제어방출형 약물수송체

황성주,이계주,조항범,이기명,김종국 충남대학교 약학대학 의약품개발연구소 1993 藥學論文集 Vol.9 No.-

The purpose of this paper is to explore the possible applicability of alginate beads as an oral controlled release system of polymeric drugs. Cellulases was used as a model polymeric drug. The release of cellulase from alginate beads was moderately affected by the ratio of cellulase to sodium alginate and strongly affected by CaCl_2 concentration. However, the release was not particularly affected by the other factors such as sodium alginate concentration and curing time. The drug was not released from alginate beads at pH 1.2 but was released continuously up to 8 hr at pH 6.8. At pH 6.8, the beads were swollen highly up to 3 hr, thereafter, were eroded into the bulk solution up to 6 hr, completely. Drug release from the beads can be caused due to diffusion and erosion of the matrix. Activity of cellulase was reduced when alginate beads containing cellulase were stored in simulated gastric juice. Further investigation would be necessary to improve the acid resistance of beads. Since the release of cellulase as a model polymeric drug could be controlled by tthe regulation of the preparation conditions of alginate beads, the alginate beads may be used for a potential oral controlled release system of such polymeric drugs as polypeptide drugs.

임상 가검물에서 분리된 균(대장균과 황색 포도균)의 약제 내성

김중명,김재식,김경선,김재룡,전동석,최성만,서상철,김인자,김재숭,이건일,김경숙 慶北大學校 醫科大學 1982 慶北醫大誌 Vol.23 No.2

1981년 1월에서 12월말까지 임상 제료에서 분리한 Escherichia coli (E. coli)와 Staphylococcus aureus (S. aureus) 중에서 무작위로 각 50주 씩 선택하여 원판 확산법과 평판 희섭법으로 그 내성주를 조사하여 다음과 같은 성적을 얻었다. 전체적으로 볼 때에 Teteracyclin (TET), Chloramphenicol (CM) 및 Ampicillin (AMP)에 대한 내성주가 가장 많았고, Amikacin (AMK)에 대한 내성주는 가장 적어다. 이것을 균종별로 보면 E. coli에 있어서는 TET, AMP 및 CM는 원판 확산법으로서 74∼76%, 평판 희석법으로서는 78∼94%가 내성주임을 나타내었고, 또 Cephalothin(CEP)은 원판 확산법에서는 8%가 내성주로 나타났으나, 평판 의석법에서는 52%가 내성주로 나타났다. S. aureus에 있어서는 TET는 원판 확산법으로 82%, 평판 희석법으로서는 78%가 내성주로 나왔고 Penicillin (P)은 원판 확산법에서는 56% 평판 희석법으로는 22%의 내성주가 나왔으며, GM과 CM은 원판확산법에서는 18∼44%, 평판 희석법에서는 56∼58%가 내성주로 나왔다. Each 50 strains of Escherichia coli and Staphylococcus aureus were selected at random out of the stock cultures which were isolated from the clinical materials in the bacteriological laboratory of the department of clinical pathology. Kyungpook National University Hospital from the first of January to the end of December in 1982, and their resistance against eight antibiotics were as follows; Escherichia coli and Staphylococcus aureus had the most resistant pattern against tetracyclin, chloramphenicol, and ampicillin, while they had the most sensitive pattern against amikacin in general. Escherichia coli revealed resistant pattern to tetracyclin, ampicillin, and chloramphenicol 74-76% by the disk diffusion method and 78-94% by the plate dilution method, and revealed resistant pattern to cephalothin 8% by the disk diffusion and 52% by the plate dilution. Staphylococcs aureus disclosed resistant pattern to tetracyclin 82% by the disk diffusion and 78% by the plate dilution, and disclosed resistant pattern to penicillin 56% by the disk diffusion and 22% by the plate dilution, and to gentamicin and-chloramphenicol 18-44% by the disk diffusion and 56-58% by the plate dilution.

차량용 Printed On-Glass 안테나에 관한 연구

박상명,이중근 漢陽大學校 工學技術硏究所 1998 工學技術論文集 Vol.7 No.1

본 논문에서는 차량 뒷 유리와 열선과 유사하게 차 유리 표면에 패턴으로 실장 되어 사용 될 수 있는 Printed On-Glass 안테나의 특성을 해석 하였다. 먼저 도체 판을 동일한 크기의 세그먼트로 분할하고, 각각에 전류와 전하의 기저 함수를 가정 한 후 모멘트 법(Moment Method)을 사용하여 도체 판에 대한 산란 문제를 해석하였다. 또한 동일한 알고리즘을 Printed On-Glass 안테나 해석에 적용하였으며, 연산의 수를 줄이기 위해 안테나 요소(Element)를 세그먼트로 분할할 때 안테나 구조를 고려하여 각 세그먼트의 크기를 달리하여 해석하였다. 시뮬레이션 된 안테나 패턴상의 전류 분포로부터 안테나 임피던스와 복사 패턴을 계산하였으며, 시뮬레이션과 실제 측정치를 비교한 결과 서로 유사함을 알 수 있었다. The objective of this paper is in the analysis and simulation of the characteristics of an automobile printed On-Glass antenna. With numerical methods (Moment Method), scattering problems on a finite flat conducting plate are analyzed by segmentation of the conducting plates, and assumed a current and charge basis function for each segment. Then these algorithms are applied to a printed On-Glass antenna problem. Segment sizes were adaptively chosen to save the calculation times. From the simulated current distribution on antenna patterns, antenna impedances are calculated, and radiation patterns are simulated. Then these are compared with the measured values, which turned out to be similar to each other.

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.