三天池의 環境과 微生物의 多樣性

한상국,이종운,배소현,장무웅,김시균 嶺南大學校 環境問題硏究所 1993 環境硏究 Vol.13 No.1

경상북도 경산시에 위치하고 있는 삼천지에 대한 환경요인 및 미생물상에 관하여 조사하였다. 삼천지 수계의 환경요인 분석결과, 그 평균값은 기온이 25.8℃, pH6.77, 경도 117.94mg/ℓ, 부유물질 4.5mg/ℓ, 용존산소량 3.3mg/ℓ, 생물학적 산소요구량 6.6mg/ℓ, 화학적 산쇼요구량 7.2㎎/ℓ, 염소이온 17.56㎎/ℓ, 황산이온 7.14㎎/ℓ로 나타났고, 중금속류는 Mn과 Zn만이 소량 검출되었으나 전반적인 오염도가 높아 수질환경 기준 IV급수에 해당하였다. 총 세균수는 3.32-8.85×?? No. inds/㎖의 분포를 나타냈고, 식물플랑크톤의 종구성은 규조류 33종, 녹조류 26종, 남조류 8종, 유글레나 13종, 와편모조류 6종 등 86종이 확인되었다. 규조류중 띠돌말과에 속하는 Fragilaria capucina, Synedra acus, S. familiaris 가 우점종이였다. Environmental factors and microflora of Samchun-impoundment in Kyongsan, Kyongpook were studied. Average data of environmental factors analysis are as followings : air temperature 25.8℃, pH 6.77, total hardness 117.94mg/ℓ, SS 4.5mg/ℓ, DO 3.3mg/ℓ, BOD 6.6mg/ℓ, COD 7.2mg/ℓ, ?? 17.56mg/ℓ, ?? 7.14mg/ℓ, Mn 0.05mg/ℓ, Zn 0.04mg/ℓ. Total bacteria in 4 site were 3.32×?? No. inds/㎖. Distribution and species of phytoplankton and zooplankton were 86 species including 33 species of bacillariophyceae, 26 of chlorophyta, 8 of cyanophyceae, 13 of euglenaceae, 6 of dinophyceae. Fragilaria capucina, Synedra acus, S. familiaris were dominant species.

領空主權과 하늘의 自由

韓成均 건국대학교 1974 樂圓誌 Vol.9 No.1

HPLC를 이용한 Methomyl의 측정법에 관한 연구

김택균,이상한,조만희,김창세 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.2

The measurements of pesticide Methomyl were studied and following conditions were proposed as a most good methods: 1. There were detected at 233 nm by means of Hitachi 200-20 spectrophotometer. 2. Pesticide Methomyl measurement was performed by means of ISCO model 2350, spherisorb ODS column, 40% methanol as a solvent phase, flow rate 0.5ml/min, chart speed 0.5 cm/min, and 25C oven temperature. 3. The relationships between the concentration and absorbance of pesticide Methomyl were linear from 2.5 to 15 ppm. 4. Deproteinzation would be used satisfactorily in all of Folin-Wu, trichloracetic acid and perchloric acid, and the recovery rate was also satisfactorily for all of them.

보행속력과 동작의 부드러움과의 상관관계에 관한 연구

탁계래,한영민,최진승,이정한,임영태,전재훈,박상균,박승하,Stefanyshin Darren 한국운동역학회 2006 한국운동역학회지 Vol.16 No.1

Creutzfeldt-Jacob병 1례

홍승철,한진희,정종현,김소양,허균,이성필 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

저자들은 Creutzfeldt-Jacob병 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. 환자는 약 4개월에 걸친 비교적 짧은 기간 동안 중증의 인지 기능 장애와 시각 장애, 보행장애와 하지의 약화, 불수의적 운동 등과 함께 섬망상태에까지 이르게 되어 본원 정신과에 입원하게 되었다. 정신상태 검사상, 반응시간의 지체, 언어장애와 보속증, 시간에 대한 지남력 장애, 주의 집중력 장애, 단기 및 장기 기억 장애, 추상적 사고능력 장애 등의 소견을 보였고, 이러한 증상들은 입원후 치료에도 불구하고 빠르게 악화되었다. 뇌파검사상 지속적인 다발초점성 서파가 모든 전극에서, 특히 우측 전두부에서 현저하게 나타나는 소견을 보였고, 병리조직학적 검사상 대뇌 피질에 신경손실을 동반한 해면화와 신경교 증식증을 나타냈다. 이상의 소견들은 모두 CJD의 진단에 합당하였다. We report a case of Creutzfeldt-Jacob disease and review the relevant literatures. This 56-year-old male patient was admitted to our hospital with a history of rapidly progressing cognitive dysfunction, visual disturbance, gait disturbance, weakness, involuntary movement of lower extremities, and the symptoms of delirium. On mental status examination, he showed psychomotor retardation, speech disturbance, perseveration, disorientation, impairment of short-term and long-term memory, and inability of abstract thinking. He had a rapid downhill course, along with shakiness of trunk, rigidity of the limbs, myoclonus, confusion, and finally, inability to speak or move on the bedside. On electroencephalogram(EEG), continuous multifocal show delta activities were found in all leads, especially in the right frontal areas. Brain biopsy showed, spongiosis with neuronal loss and gliosis noted at the cerebral cortex. These findings were compatible with Creutzfeldt-Jacob disease.

유방의 섬유낭종성 질환에서 보이는 아형의 빈도에 대한 연구

은약수,김의한,강상균 순천향대학교 1993 논문집 Vol.16 No.2

To understand the incident rate about the subtype of fibrocystic disease, we collected 116 breast cases requested from Jan. 1991 to June 1992, and reviewed, The results were as follows: 1. The fibrocystic disease was observed in 52.5%(61 of 116 cases). 2. The peak incidence was in the third decade and it did not reveal before the age of 20 and after the age of 50. 3. In fibrocystic disease, epithelial hyperplasia relative to breast cancer was observed in 33%(19 of 61 cases)and the epithelial hyperplasia was more significant in the young age group. 4. Among the 61 cases of fibrocystic disease, the most frequent type was fibrosis (88.5%), and followed by microcyst(32.8%), ductal hyperplasia(24.6%), lobular hyperplasia(11.5%), apocrine metaplasia(9.8%) and macrocyst and sclerosing adenosis(1.6%) in order.

Paraquat 투여에 의한 가토 간장의 변화에 관한 연구

이종민,김의한,강상균 순천향대학교 1992 논문집 Vol.15 No.2

Paraquat(이하 PQ)에 의한 간장의 변화를 알아 보기 위하여 25마리의 가토에 PQ를 1㎏당 10㎎, 20㎎, 40㎎, 80㎎, 160㎎을 복강 및 정맥주사하여 G. P. T와 G. O. T치를 측정하고 간장의 조직소견과 비교 관찰하여 다음과 같은 결론을 얻었다. 1) PQ에 의한 G. P. T와 G. O. T의 변화는 일단 독성을 일으킬 만한 양이 투여되면 투여양이나 투여방법에 상관없이 투여 후 바로 상승하여 3∼4일 지속되다가 떨어진 후 7∼9일 후 다시 상승하는데 정맥주사한 예에서 조금 빠르게 나타나는 양상을 보였다. 2) PQ투여로 인한 간장의 변화가 직접적인 사망원인으로 작용하는 것 같지는 않으나 적은예에서 죽기 직전 심한 G. P. T와 G. O. T의 상승을 보이는 경우가 있어 PQ로 인한 사망원인에 하나의 인자로써 작용하는 것 같았다. 3) PQ로 인한 간장의 조직학적소견은 전반적인 울혈을 볼 수 있고 3일이상 생존한 가토의 심정맥 주위에서 세포 종창, 지방변성, 풍선화 및 간소엽의 산란등이 주로 관찰되며 변화의 정도는 투여양이나 생화학적 변화와 비례해서 나타나지 않았다. To investigate the effect of PQ on the liver, PQ was injected at dose of 100mg/kg, 20mg/kg, 40mg/kg, 80mg/kg, 160mg/kg intraperitoneally and 20mg/kg, 40mg/kg, 80mg/kg intravenously. The changes in hepatic funtion were estimated by determining G.P.T. and G.O.T. and compared to histologic findings of liver tissue. From these, we concluded : 1. Once sufficient toxic concetration of PQ is administrated, PQ led to increase in GPT. and GOT, which lasts for 3-4days regardless of the route and amount of adminstration. The gradual return of the GPT and GOT to the upper normal limit was followed. After 7-9days, the GPT and GOT level is again elevated. The occurence of elevation in GPT and GOT appeared earlier when intravenous injection than intraperitoneal injection. 2. The hepatic change by PQ was not the direct cause of death, though occasionally observed marked GPT and GOT elevation prior to death. It is likely that the liver damage is a contributing factor causing death in PQ intoxication. 3. Histologically, hepatic changes such as fatty change, ballooning degeneration, cell cord disarray were mostly limited in the centrilobular area in the rabbits survived for more than 3days. The degree of histological hepatic changes was not necessarily proportional to either the dose of PQ or the biochemical level.

Paraquat 투여로 인한 흰쥐 폐장변화에 대한 면역 조직화학적 연구

도재원,김의한,강상균 순천향대학교 1992 논문집 Vol.15 No.2

Paraquat에 의해서 유발되는 간질 및 폐포내 섬유조직 증식증의 진행과정을 알아보기 위하여 20마리의 흰쥐를 대상으로 10mg/kg, 20mg/kg를 복강내 주사하고 3일, 5일, 7일, 10일, 15일 간격으로 폐장을 적출하여 조직학적 및 면역조직 화학적 방법을 통해 관찰하여 다음과 같은 결론을 얻었다. 1. 10mg/kg를 투여한 군에서는 뚜렷한 손상을 보이지 않고 7일후에는 거의 정상으로 나타나 Paraquat에 의한 독성 효과는 투여된 양에 의존됨을 알 수 있었다. 2. 나타나는 폐장의 변화는 2단계로 분류될수 있는데 폐포내에 출혈, 부종 및 초자막 형성은 초기에 나타나는 병변이었으며, 간질 및 폐포내 섬유 조직 증식증은 후기에 나타나는 병변으로 일반적인 급성 호흡곤란 증후군때 보이는 소견과 비슷하였다. 3. 관찰 초기부터 폐포세포 Ⅱ형의 증식이 나타나는 것으로 보아 중독 초기에 이미 폐포세포 Ⅰ형에 손상이 나타남을 알 수 있었다. 4. 간질 및 폐포내 간엽조직은 시간이 경과됨에 따라 Vimentin에 강하게 염색되는 것을 볼 수 있는데 이는 간질 및 폐포내 섬유조직 증식증이 시간 경과에 따라 진행되고 있음을 의미한다. 5. 7일 이후에 상피세포가 다시 EMA에 강한 양성 반응을 보였으며 이는 이들 세포들이 재생되고 있음을 뜻하고 있으나 더욱 시간을 두고 관찰 할 필요가 있다고 본다. To investigate the progress of the Paraquat-induced pulminary interstitial and intralveolar fibrosis, 10 white rats were administered 10mg/kg and 10 white rats were administered 20mg/kg of Paraquat intraperitoneally. Three, five, seven, ten and fifteen days after this injection, the rats were sacrificed. The removed lungs were evaluated by immunohistological and histological study, so following results were obtained. 1. Toxic effects of Paraquat seem to depend on the dose of administrated Paraquat since the groups of rats treated with 10mg/kg revealed no significant tissue damage after 7 days. 2. The pulmonary changes by Paraquat can be classified into two stages : the early change such as hemorrhage, edema and hyaline membrane formation, and the late change characterized by interstitial and intra-alveolar fibrosis. These changes are similar to the findings observed in acute respiratory distrees syndrome. 3. Proliferation of Type Ⅱ alveolar cell at the time of the initial observation (3days) suggested that the damage of type Ⅰ alveolar cell had been occured prior to observation. 4. Strong Vimentin positive staining of the interstitial and intra-alveolar mesenchymal cell with time indicated the progression of the interstitial and intra-alveolar fibrosis. 5. Strong positive reaction of epithelial cells to EMA after 7 days of Paraquat adminstration indicated the regeneration of these cells. However, due to short duration of observation, it was hard to obtain conclusion whether this regeneration completed or not.

쥐의 피아조직에서 Nitionl과 Stainless steel의 삽입으로 인한 조직반응에 대한 비교 연구

나춘균,강상균,박희주,김의한 순천향대학교 1994 논문집 Vol.17 No.2

An experimental study was carried out to compare the tissue reaction of nitinol with that of stainless steel. 0.5cm, 27 nitinol wires(A group) and 0.5cm, 27 stainless steel wires(B group) were inserted into the beneath back skin of each 54 white rat and examined for 8 weeks. 3, 5, 7, 10 days, and 2, 3, 4, 6, and 8 weeks after the insertion, 6 rats(3 nitinolinserted rats and 3 stainless steel-inserted rats) were sacrificed and examined to make emphasis on such microscopic points as 1) vascular proliferation 2) type of infiltrated inflammatory cells, 3) proliferation of fibroblasts and collagen 4) the thickness of the membrane surrounding implants and 5) epidermal growth factor reactivity. As a result, the vascular proliferation and fibroblastic and collagen proliferation appeared to be somewhat earlier in the A group than in B group and the thickness of membrane was slight thicker in A group than in B group at an early stage. However, the infiltrated inflammatory cells showed no difference between both A and B groups. The above findings suggested that formation of the granultion tissue appeared somewhat earlier in the A group, but there were no significant meanings on microscopic grounds between them. Insertion in both A and B groups reflected the most intense reactivity of EFG after 3-5 days. The results that the reactivity of EGF was more intense at the periphery of the membrane after 2-3 weeks in bath A and B group were given in that the production of fibroblasts and collagen were revealed chiefly at the periphery of the membrane adjacent to nitinol and stainless steel.

유방암에서 Peanut lectin과 Cystol Estrogen Receptor 의 분포에 관한 비교연구

박희주,김의한,강상균 순천향대학교 1991 논문집 Vol.14 No.2

To understand interrelationship between localization of Peanut lectin and Cytosol Estrogen Recepter, the authors collected 25 cases female breast carcinoma obtained by the biopsy and mastectomy, We performed H & E stain and immunohistochemical stains by Avidin-Biotion-Complex method then analyzed their reactions. Results obtained are as following : 1. Of 25 patients with breast cancer, 15 were presented with PNA-positive cells in their tumor tissue, However, there were variation in incidence for PNA in the tumor cells depending on the degree of differentiation ; while PNA-positive cells were observed in all patients with well differentiated tumor(N=3/3), they were displayed in 50% of patients with poorly differentiated tumor(N=5/ 10). In patients with tumor with moderate differentiation. PNA-positive cells were present in 55.6%(N=5/ 9). It appears that the better the differentiation, not only the higher the incidence for PNA at the free border of cell membrance. 2. Of 25 brest cancer, 14 were presented with CER-positive cells. CER-positive cells were exhibited in 33.3%, 66.7% and 70.0% in well differentiated(N=1/ 3), moderate differentiated(N=6/9), and poorly differentiated(7/10), respectively. On view of the degree of differentiation of the tumor cells. CER-positive and PNA-positive cells reveals opposite pattern. 3. There was a some relationship between age and incidence for PNA or CER in tumor cells. In patients at age less than 40, between 40 and 50 and over 50, CER positivity in tumor cells were 13.3%, 33.3% and 53%, and PNA positivity in tumor cells were 7.1%, 36% and 57%, respectively. 4. 8 cases of PNA positivity(57.1%) was detected among 14 cases of CER positive cases, while 7 cases(63.6%) of PNA positivity was noted among 11 cases of CER negative. There the expression pattern of PNA and CER were different and unrelated each other. 5. Although stronger or more extensive staining for CER tended to be associated weaker or more restricted staining for PNA, the tendency for reciprocal correlation could not quite achieve the statistial significance.