녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

성인에서 발생한 발진성 가성 혈관종증

서상희 ( Sang Hee Seo ),장호선 ( Ho Sun Jang ),목혜수 ( Hye Soo Mok ),김성준 ( Sung Jun Kim ),김병수 ( Byung Soo Kim ),김문범 ( Moon Bum Kim ),오창근 ( Chang Keun Oh ),권경술 ( Kyung Sool Kwon ) 대한피부과학회 2007 대한피부과학회지 Vol.45 No.8

Background: Eruptive pseudoangiomatosis (EPA) is a rare, benign, spontaneously regressing childhood exanthem. It is characterized by the sudden onset of several bright red angioma-like papules surrounded by pale halos with a distinct histopathology from true angiomas. Objective: This study was performed to evaluate the clinical and histopathologic characteristics of EPA occuring in adults. Methods: Ten adult patients who visited Pusan National University Hospital and Mok Hye-SooㆍJang Ho-Sun Dermatology Clinic from March 2005 to September 2006 were evaluated. We prospectively evaluated the sex, age, onset season, past medical history including immunosuppressive abnormalities, systemic disorders and other diseases including allergies. We also investigated the relations of mosquito biting, patients` occupations and outdoor activities to occurrence of EPA. In addition, simultaneous occurrence in family members, the clinical, histopathologic, laboratory findings, disease courses and responses to treatment were evaluated. Based on medical records, photographs and pathologic slides, we retrospectively diagnosed another 20 EPA patients suspected as insect bite from October 2003 to March 2005. The same questions were inquired as for the 10 patients who prospectively underwent evaluation. Results: In the study, female predominance (76.7%) was observed and the average age of onset was 54.2 years. Interestingly, there was no child patient during the period of study. Multiple, 2∼5 mm sized, red angiomatous papules surrounded by pale halos occurred on exposed areas such as the arms (86.7%), legs (50%), and face (46.7%), although it could also occur to a non-exposed area. EPA occuring in adults usually appeared in summer (80%). The mean disease duration was 3.4 weeks. Although EPA spontaneously regressed, it had the potential of recurrence (46.7%). Histopathologic findings showed dilated dermal blood vessels without the evidence of increase in numbers, and perivascular lymphocytes infiltration. Inside the lumen of dermal blood vessels, plump endothelial cells were found. Conclusion: EPA occuring in adults usually happened to exposed sites in summer, so it can be misdiagnosed as insect bite. We suggest that dermatologists should be concerned about EPA in adults and conduct further investigation to have a better understanding of the disease. (Korean J Dermatol 2007;45(8):797∼803)

답손(Dapsone)중독환자의 예후와 특성 분석

장석준,정성필,김승환,구홍두,심호식 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

We reviewed 26 dapsone intoxication patients who had visited Severance Hospital Emergency Care Center from January 1991 to January 1995 : 13 of them ingested intentionally, but the other ingested accidentally. Among the admitted patients the highest and lowtheest initial value of methemoglobin level is 59.6% & 10.1%. The frequency of symptoms at presentation was following 16 patients complained cyanosis of lip area, 10 patients complained dyspnea, 4 patients complained mental change, 4 patients complained vomiting, 2 patients complained nausea. The inital level of methemoglobin was less than 50% except in one patient. In the view points of total doses of methylene blue used, less than 100mg was used in 9 patients(34.6%) but in one case, amount of 6400mg was loaded. There were 5 patients(19.2%) in September & 4 patients (15.4%) in January. In respect of result, there were 13 discharged cases (50%) for symptom improvement, and 5 mortality cases(19.2%). The transferred cases from Gangwondo account 19 patients(73.1%). All of them is not directly emergency care center visiting patients but transferred patients from remotely located hospitals. Dapsone intoxication results in severe tissue hypoxic symptoms from malfunction of normal hemoglobin thus only with appropriate available doses of methylene blue & activated charcoal, good outcomes will be expected. If aggressive therapy such as hemodialysis is introduced in treatment of severe dapsone intoxication, the outcomes may be slightly improved better than in other supportive care cases.

레슬링 선수들의 정신건강에 관한 연구

장호성,최종균,구용판 龍仁大學校 體育科學硏究所 2003 體育科學硏究論叢 Vol.13 No.1

The purpose of this study is to determine how a wrestler's sex, age, match experience and education level relate to his/her psychological health. A sample group was formed from 197 male and female players (senior high-school level or higher) who participated in the 2002 Korean Wrestling Tournament. The players are registered with the Korea Wrestling Federation. The questionnaire, which contained total 94 questions (i.e., 4 on sociodemographic characteristics and 90 on psychological health-related factors), showed the confidence level falling between .5006 and .8844 (Cronbach's alpha). The study, which relied on frequency analysis, t-test, One-way ANOVA, and Scheffe test, produced the following results. First, wrestlers' psychological health showed no statistically significant difference with regard to all sub-variables depending on sex. Second, wrestlers' psychological health showed a statistically significant difference regarding overall psychological health, somatizing disorder, obsession, oversensitivity to others, depression and hostility depending on age while no difference was noticed regarding insecurity, fear, paranoia and psychosis. Third, wrestlers' psychological health showed a statistically significant difference regarding overall psychological health, somatizing disorder, obsession, depression, paranoia and psychosis depending on match experience while no difference was noticed regarding oversensitivity to others, insecurity, hostility and fear. Fourth, wrestlers' psychological health showed a statistically significant difference regarding overall psychological health, somatizing disorder, obsession, depression, insecurity and hostility depending on education level while no difference was noticed regarding oversensitivity to others, fear, paranoia and psychosis.

설악산 국립공원의 어류분포

장민호,조가익,하진용,정광석,박성배,주기재 한국어류학회 2002 韓國魚類學會誌 Vol.14 No.4

설악산 국립공원지역 산간계류의 17개 지점에서 2000년 6월부터 2001년 6월까지 어류상 및 이화학적 요인을 계절별로 총 4회에 걸쳐 분석하였다. 모든 조사지점은 1~3차의 하천으로 비교적 물리·화학적 교란이 적었으며 하천폭은 5~30 m 정도였다. 채집된 어류는 총 18과 42종 2,557개체였고, Cyprinidae는 16종으로 84.7%의 상대풍부도를 나타냈으며, Gobiidae 4종, 5.0?의 상대풍부도를 보였다. Cyprinidae는 전체 채입종의 37.2%를 나타냈다. 우점종은 Zacco temminck로 10개 지점에서 738개체가 채집되어 28.9%의 상대풍부도를 나타냈으며, 아우점종은 Rhynchocypris kumkangensis와 Z. platypus로 각각 4개 지점 648개체(25.3%)와 7개 지점 344개체(13.5%)로 나타났다. 한국고유종(Korean endemic species)은 모두 4과 11종 781개체로 30.5%의 상대풍부도를 나타냈으며, 고유화 빈도는 25.6%로 한국의 평균 고유화 빈도와 유사하게 나타났다. 본 조사에서 외래어는 채집되지 않았다. 국립공원 내에 서식하는 어류의 다양성 유지와 회복을 위해서는 공원 내는 물론 경계지역 하천의 체계적인 관리가 요구된다. The fish fauna of mountain streams in the Seorak National Park area was investigated from June 2000 to June 2001 (4 times). All 17 sites were relatively undisturbed 1st to 3rd order streams. A total of 2,557 individuals were collected and classified into 17 families and 43 species. Cyprinidae accounted for 37.2% (16 species) of the total fish species and 84.7% (2,165 ind.) of the total abundance. Subdominant families were Gobiidae (4 species, 5.0%) and Balitoridae (3 species, 3.4%). Zacco temmincki (RA 28.9%, 10 sites) dominated. Subdominant species were Rhynchocypris kumkangensis (25.3%, 4 sites) and Zacco platypus (13.5%, 7 sites). Eleven of the species are endemic to Korea (4 families, 30.5%). Exotic species were not collected in this survey. Fish diversity was higher in streams around the boundary area than in the national park. Therefore, management of streams in the boundary area to protect the fish biodiversity can be efficiently conducted. Habitat degradation should be reduced for the conservation and restoration of fish diversity.

축력과 양단 대칭모멘트를 받는 스테인리스 각형강관 기둥의 좌굴내력에 관한 실험적 연구

장호주,서성연,양영성 대한건축학회 2005 大韓建築學會論文集 : 構造系 Vol.21 No.2

This study is a series of experimental investigation on the buckling strengths of eccentrically compressed cold-formed stainless steel square hollow section columns. The principal parameters in this study are slenderness ratios(λ=30, 50, 70) and magnitude of eccentricity e(0, 2.5, 5.0, 7.5, 10cm) on the symmetrical end-moment. Objectives of this paper are to obtain the maximum loads by performing an experimental work and to compare the experimental behaviors with analytical results. The ultimate buckling strength of square section members are evaluated by a numerical method in accordance with the bending moment-axial force(M-P) interaction curves. The behavior of each specimen is displayed in the form of moment-angle(M-θ) relationship. The numerically obtained ultimate buckling interaction curves of beam-columns showed a reasonably good agreement with the available experimental results.

천연가스 사용 난방 및 산업보일러의 NOx와 CO 배출계수 산정 연구

장영기,최상진,김관,송기봉,김호정,정봉진 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.5

This study was conducted for developing the emission factors of nitrogen oxide(NOx) and carbon monoxide (CO) from the combustion boilers burning liquefied natural gas (LNG). These emission factors were compared with those of U.S. EPA and European Environment Agency (EEA). NOx and CO concentration in the flow gas were measured using Kane-May, KM9106 and Thermo Environmental Instruments Inc., 42C-HL. Measurement were conducted at thirty industrial and commercial LNG boilers. Emission factors were calculated on the basis of fuel consumption (kg-pollutant/㎥-fuel burned). NOx concentration at industrial boiler was 14~125 ppm and it was measured as 35~125 ppm at commercial boiler. NOx emission factors of industrial boiler and commercial boiler were 1.84kg/㎥ and 2.09kg/㎥, respectively. NOx emission factor of commercial boiler was higher than that of industrial boiler. The NOx emission factors estimated in this study were lower than those of U.S. EPA and higher than those of EEA. Average CO emission factor of industrial boiler was 0.65 kg/㎥ and at commercial boiler it was 0.70kg/㎥, CO emission factor at industrial boiler was lower than that at commercial boiler.

일부 남성 금속제품 조립작업자들의 피로에 영향을 미치는 작업관련요인

장준호,강동묵,고상백,김정원,조병만,이수일 大韓産業醫學會 2004 대한직업환경의학회지 Vol.16 No.2

목적 : 남성 금속제품 조립 작업자들의 피로에 영향을 미치는 작업관련요인을 파악하고자 한다. 방법 : 작업자의 일반적 특성 및 작업관련 특성, 육체적 부하, 직무 스트레스 등을 조사 항목으로 하여 자동차 부품 조립업 1개소(476명), 자동차 엔진 조립업 1개소(610명), 대형 선박 엔진 조립업 1개소(454명)의 작업자(1,540명)를 대상으로 수거된 설문지 중 부실하게 응답한 38부를 제외한 1,136부(총연구대상의 73.8%)를 분석하였고, 피로도와의 연관성을 다중선형회귀분석으로 살펴보았다. 조사는 자기 기입식 설문지를 사용하였다. 육체적 부하를 측정하기 위해서는 Borg의 scale이 사용되었다. 격무 스트레스를 측정하기 위해서는 Karasek의 Job content questionnaire가 사용되었다. 피로의 측정은 전반적 피로도(sum of fatigue), 일상생활 기능장애(daily dysfunctioning), 상황적 피로(situational fatigue), 피로점수의 총합(sum of fatigue)으로 구성되어 있는 다면적 피로척도를 사용하였다. 피로점수의 분포는 본 연구에 응답한 피로점수의 총합을 사분위수로 나누어서 4등급으로 나누었다. 결과 : 피로점수의 분포는 피로점수의 총합을 사분위수로 나누었을 때, 0≤1등급≤73, 74≤2등급≤82, 83≤3등급≤93, 94≤4등급≤133이었다. 다중선형회귀분석에서 피로점수의 총합과 유의한 관계를 보인 경우는 주간근무, 긴 주당 노동시간, 높은 육체적 부하, 높은 직무요구도, 낮은 직무자율성, 낮은 상사의 지지 등이었고, 전반적 피로도와 유의한 관계를 보인 경우는 주간근무, 높은 육체적 부하, 높은 직무요구도, 낮은 직무자율성, 낮은 상사의 지지 등이었다. 일상생활 기능장애와 유의한 관계를 보인 경우는 주간근무, 긴 주당 노동시간, 높은 육체적 부하, 낮은 직무자율성 등이었다. 상황적 피로와 유의한 관계를 보인 경우는 높은 연령, 주간근무, 긴 주당 노동시간, 낮은 직무자율성 등이었다. 결론 : 피로관련 요인은 긴 주당노동시간, 높은 직무요구도, 높은 육체적 부하, 낮은 직무자율성, 낮은 상사의 지지로 생각된다. Objectives: To find work related factors affecting perceived fatigue among male metal product assemblers. Methods: The study subjects were metal product assemblers from three metal product companies (476 car parts assemblers, 610 car engine assemblers, 454 ship engine assemblers). From the total of 1,540 subjects, 1,136 (73.8%) were included and 38 respondents with insufficient replies were excluded. Associations between fatigue score and other variables (general characteristics, job related factors, workload, job stresses) were investigated in terms of the concept of multidimensional fatigue construct by self administered questionnaire. Using MFS (Multidimensional Fatigue Scale), JCQ (Job Content Questionnaire), and Borg's scale, we estimated the fatigue, job stress, and workload, respectively. Fatigue score was estimated by 'sum of fatigue' in MFS. Associations were tested by linear regression analysis using SAS v.8.0. Results: In this study, the fatigue related factors were older age, longer working hours per week, higher workload, higher job demand, lower decision latitude, and lower supervisor support. Conclusions: Prolonged working hours, higher workload, higher job demand, poor supervisor support and lower decision latitude were fatigue related factors among the male metal product assemblers of this study.

사진을 활용한 시 창작 교육의 가능성

장화심,유성호 한국교원대학교교육연구원 2006 敎員敎育 Vol.22 No.3

창작은 더 이상 전문가들만의 것이 아니라 학습 과정에 있는 창작 주체 즉 학습 작가가 수행하는 문학적 글쓰기의 과정과 결과를 포함해야 하며, 따라서 '창작 교육'은 전문적인 작가를 배출하는 것을 목적으로 하는 것이 아니라 학습자의 문학적인 창작의 욕구를 자연스럽게 분출하여 생산 하도록 해야 한다. 따라서 시 창작 교육에서도 쓰는 경험을 제공하고 표현 과정을 중시해야 한다. 그래서 시 창작 교육은 단순한 감정 표현만이 아니라 인식의 차원에서 이루어지는 세상 읽기 교육이 되어야 하며, 경험이 부족한 학생들에게 인식의 폭을 넓히고 세상 읽기가 가능하도록 해야 한다. 이때 다양한 매체를 활용하면 효과가 배가된다. 여기서 사진의 매체적 특성은 매우 시사적인 가능성을 준다고 할 수 있다. 요컨대 사진을 활용한 교수 학습 활동은 사진 텍스트 읽기와 시 창작을 결합시키는 일이 될 것이다. 사진 안에는 내 삶을 돌아보게 만드는 남의 삶이 담겨 있다. 사진을 보고 학습자 자신의 사고를 글로 표현하는 과정에서 삶과 세계를 이해하고 주위 대상에 관심을 갖게 되며, 대상의 의미 해석을 통해 자신의 세계관을 확대하고 바람직한 세계관을 형성할 수 있다. 즉 간접 체험을 통해 인생과 삶에 대한 폭넓은 이해를 쌓고 나아가 자신의 삶을 되돌아보고 미래의 행동 지침으로 삼게 만들 수 있는 것이다. 또 사진을 보고 시를 창작하는 활동은 '읽기'와 '쓰기', '이해'와 '표현'이라는 상반된 것처럼 생각되었던 국어 교육의 내용을 결합하는 통합적 교육 효과를 얻을 수 있을 것이다. 또 인쇄 매체에 한정된 기존의 '읽기' 개념을 더 확장하여 문화 읽기로 나아가며, 작지만 매체의 변화를 고려한 국어 수업이 될 수 있을 것이다. Literature education needs to contribute to the fostering of a self-directed and active person. The crux of developing a self-directed and active ego-formation can be reached by encouraging students to engage in creative writing. However, literature education has been focused on how the literary works should be understood and appreciated. Literature education is introduced in the 7th national curriculum as an attempt to realize the ultimate goal of literature education, but has not been carried out well in the classrooms of individual schools. The part of' Literature reception and creative writing' includes' creative reconstruction of a literary work' and' creative writing of literature', in which the art of creative writing through' imitation' and' revision' is presented in the content organizations in the area of literature in the high school curriculum. In order to overcome this limitation of creative writing without any teaching method, a variety of attempts have been carried out. Among them, there are a great many of creative writing teaching methods through the formal elements, such as metaphor and rhythm, and techniques of expression. But, it is writing materials, not poetic expression that counts in the creative writing of poetry. Researches on mind-map, brainstorming, and free association as methods to make writing materials rich have been conducted, but it's difficult to draw pedagogical implications from the result of these researches for teaching creative writing to students with limited experience and knowledge. The purpose of this study is to present and propose a method of literature education that can engage the students in creative writing of poetry along with literature appreciation. However, this study shows a few limitations: not dealing with all the processes and methods for students' idea-making in a creative writing class, not analyzing the quantitative data showing the substantial improvement level of the students, and restricting them within the qualitative analysis of concrete situations of teaching of idea-making through the interaction between the teacher and the students.

鹽-觸媒와 Phenyl Glycidyl Ether에 依한 絹纖維의 改質에 관한 硏究

張炳浩,李相洛 成均館大學校 科學技術硏究所 1988 論文集 Vol.39 No.2

The silk was padded with NaSCN containing to an approximate 100% pick up and then treated with solution PGE in various solvents at 75℃ for 6 hours. The conclusions were as follows; 1) PGE add-on reached maximum when 1N NaSCN, 1.5M PGE and 100% moisture regain were used in ethanol. 2) The dry wrinkle recovery angle, chemical resistance is ere increased continuously with the increase of PGE add-on. 3) Amount of amino acid in silk was decreased as PGE add-on increased. Especially, tyrosine was decreased remarkably. 4) The effect of K/S and PGE add-on were the most outstanding when n-Hexane was used in the various solvents.