Prevalence and antimicrobial susceptibility of Salmonella isolates in Pekin ducks from South Korea

Cha, S.Y.,Kang, M.,Yoon, R.H.,Park, C.K.,Moon, O.K.,Jang, H.K. Pergamon Press 2013 Comparative immunology, microbiology and infectiou Vol.36 No.5

An investigation was carried out to determine the prevalence and antibiotic resistance of Salmonella serotypes at South Korean duck farms. A total of 7119 samples collected from 72 duck farms in five provinces were examined from 2011 to 2012. The overall prevalence of Salmonella serotypes was 43.4% (69/159) in duck flocks from 65.2% (47/72) of the duck farms. Eighty-five strains were isolated from 69 duck flocks. Three serotypes of Salmonella enterica were identified such as S. Typhimurium (39/85), S. Enteritidis (44/85), and S. London (2/85). The prevalence of Salmonella infection decreased significantly in 3-week-old ducks compared to that in 1-week-old ducks (P<0.05). All isolates except one were resistant to at least one antimicrobial and 27% of the isolates were resistant to 5-16 antimicrobials. Our findings provide baseline information on the degree of Salmonella infection and distribution of Salmonella serotypes in ducks and indicate that ducks should be considered an important source of foodborne pathogens.

Comparison of mRNA, Protein, and Urinary Nucleic Acid Levels of S100A8 and S100A9 between Prostate Cancer and BPH

Yun, S. J.,Yan, C.,Jeong, P.,Kang, H. W.,Kim, Y. H.,Kim, E. A.,Lee, O. J.,Kim, W. T.,Moon, S. K.,Kim, I. Y. Springer Science + Business Media 2015 Annals of Surgical Oncology Vol.22 No.7

조직구성 수성 망상증 1예

나한식,조경상,김종두,이승일,문철웅,이호영,장숙진 朝鮮大學校 附設 醫學硏究所 1989 The Medical Journal of Chosun University Vol.14 No.2

Histiocytic medullary reticulosis is a clinicopathologic syndrome characterized by its acute onset and relentless progression to death within a few months. The major clinical features are fever, malaise, weakness, weight loss, lymphadenopathy, hepatosplenomegaly, jaundice and purpura. The common laboratory findings are severe anemia, leukopenia, and thrombocytopenia. Histologically, bone marrow were infiltrated with atypical, neoplastic histiocytes, many of which had ingested large number of red blood cells, leukocyte, platelet. We experienced one case of HMR who was a 22 year old male admitted via ER with malaise, general weakness, fever and headache. The patient died often 11 days of illness. Death was due to massive bleeding and DIC thought to be due to pancytopenia secondary to massive blood element phagocytosis. So, we reported one case which considered compatible for HMR, with a few elementary reviewed literatures.

Production of F1 and Backcross Progenis from a Cross of O. sativa x O. minuta

Y. S. Cho,K. H. Kim,K. H. Kang,Y. P. Jeong,H. C. Choi,H. P. Moon,O. Y. Jeong,E. G. Jeong 한국육종학회 2002 한국육종학회 학술발표회 발표요지 Vol.34 No.1

1970年 慶北 聞慶地域에 發生한 腸티브스 疫學調査報告

李元榮,韓王洙,洪在雄,李京男,金貞順,文玉綸 서울大學校 保建大學院 1973 公衆保健雜誌 Vol.10 No.1

Epidemiology team, School of Public Health, Seoul National University, was commissioned to investigate an outbreak of unknown febrile disease occurred in Moon Kyung area from Ministry of Health. The team investigated the outbreak and collected specimens of rectal swab and sera; bacteriological study was done in National Institute of Health and serological test in the serology laboratory in the School of Public Health. Followings are the results obtained: 1. The entity of unknown febrile disease was identified as Salmonella typhi, D group. 2. Most common symptoms were headache and fever. The symptoms appeared to be quite severe in the begining of the epidemic which gradually became milder as the epidemic progressed to from of walking typhoid, probably being resulted from gradual development of population immunity. 3. It was an explosive outbreak among school children; the incidence of the disease per 1000 was 619.3 for school children whereas average incidence in four villages surveyed was 170.1. 4. The source of infection was common, open well in a corner of school ground for school children, however, the source of infection for general population was either personal contact or contaminated food in the family. 5. Distribution of date of onset and diffuse case distribution among villagers other than school children strongly suggested that these were secondary cases infected from the school children who delivered the agent to their family and/or neighbours. 6. A route of spread was assumed as follow: a beautician who travelled around extensively from one village to another for weddings was the first patient so far we could trace. She got sick in the begining of March and went home where her only brother followed her and died. she got out home and lost because of her brother's death. In the middle of March the beautician's two neighbours who shared open well contracted the disease. One of these two had such sever symptoms like delirium and aphasia that she was hospitalized in a private hospital for seven days and singed out the hospital and went her mother's home. In the begining of April her mother and one of her old classmates who was principal's son became ill. The principal lived in the school campus near the well, and thus the well got contaminated. The principal was the one who brought attention to health center because of unusually high absenteeism of the school children.

Caspase-3 activation as a key factor for HBx-transformed cell death

Kim, A.,Kwon, O. S.,Kim, S. O.,He, L.,Bae, E. Y.,Lee, M. S.,Jeong, S. J.,Shim, J. H.,Yoon, D. Y.,Kim, C. H.,Moon, A.,Kim, K. E.,Ahn, J. S.,Kim, B. Y. Blackwell Publishing Ltd 2008 Cell proliferation Vol.41 No.5

<P>Abstract. </P><P><I>Objectives</I>: Nuclear factor-kappa B (NF-&kgr;B) activation has been associated with the tumorigenic growth of hepatitis B virus X protein (HBx)-transformed cells. This study was aimed to find a key target for treatment of HBx-mediated cancers. <I>Materials and methods</I>: NF-&kgr;B activation, endoplasmic reticulum-stress (ER-stress), caspase-3 activation, and cell proliferation were evaluated after Chang/HBx cells permanently expressing HBx viral protein were treated with inhibitors of NF-&kgr;B, proteasome and DNA topoisomerase. <I>Results</I>: Inhibition of NF-&kgr;B transcriptional activity by transient transfection with mutant plasmids encoding Akt1 and glycogen synthase kinase-3&bgr; (GSK-3&bgr;), or by treatment with chemical inhibitors, wortmannin and LY294002, showed little effect on the survival of Chang/HBx cells. Furthermore, I&kgr;Bα (S32/36A) mutant plasmid or other NF-&kgr;B inhibitors, 1-pyrrolidinecarbonidithioic acid and sulphasalazine, were also shown to have little effect on the cell proliferation. By contrast, proteasome inhibitor-1 (Pro1) and MG132 enhanced the HBx-induced ER-stress response and the subsequent activation of caspase-12, -9 and -3 and reduced cell proliferation. Camptothecin (CPT), however, triggered activation of caspase-3 without induction of caspase-12, and reduced cell proliferation. In addition, CPT-induced cell death was reversed by pre-treatment with z-DEVD, a caspase-3-specific inhibitor. <I>Conclusions</I>: Detailed exploitation of the regulators of caspase-3 activation could open the gate for finding an efficient target for development of anticancer therapeutics against HBx-transformed hepatocellular carcinoma.</P>

해부실습이 의대생에게 미치는 심리적 영향과 극복과정에 대한 연구

김자예,맹선화,문옥련,유정애,이숙자,이신숙,현민숙 이화여자대학교 의과대학 1977 梨花醫學誌 Vol.- No.9

This study investigated about the adaptive behavior of medical students to specific hardship, i.e., anatomical dissection and its psychological effect upon students. The aim of this study is to help future students to be better equipped to overcome hardship and endure life as a medical students by understanding other students' adaptive behavior. This will, hopefully also, be useful in understanding how human beings keep their personality integreted by adapting themselves to circumstances under stress. The findings are restricted to individual adaptive behavior and the other important mechanisms such as the effect of peer group in adaptive behavior was not explored. The results may be summarized as follows. 1. The success of medical student life differed greatly according to their degree of goal consciousness. The most decisive factor in the degree of goal consciousness of students was the level of voluntary choice of their major field. Interestingly, more female students(58%) reported their own desion in choosing their major field than males(50%). Among the subjects of our study 54% of students came to medical school by their own choice and another 42%, partly by their own choice and partly by recommendations from others. only 4% of them came to medical school by others' pressure. As expected, most of the subjects showed goal consciousness regardless of sex of grade. 2. Most of the subjects found their medical student life difficult. 79% of premedical students and 97% of medical students reported that they were under stress. While female students replied "the atmosphere of medical student life" was the main reason for it, male students reported "the amount of work load" as the main reason. 3. Inspite of circumstance under stress, most of the students adapted themselves to the hardship, i.e., anatomical dissection. Male and female students showed no difference in their capability and ways of adaptive behavior. The most remarkable difference between males and females was their expression of emotional reaction. The ways of adaptation varied among students, some of them adapted to this circumstance affirmatively (as a means to achieve certain ends or with interest and sincerity). The others adapted to it insensibly or mechanically. A few of them could not adapt themselves at all till the end still keeping their nihilistic or guilty feeling. 7% of female students and 11% of male students revealed their inadaptability. The difference between premedical students and medical students was in their horror or guilt toward their objects of anatomical dissection. It may be due to the fact that while the premedical students performed animal dissection, the medical students performed human dissection. 4. The initial psychological conflict the students felt toward the anatomical dissection was varied. Even if students overcome it afterwork, many students doubted the necessity of becoming a medical doctor or of anatomical dissection curriculum itself. Only a few of them experienced no conflict at all. Most of students revealed changes in their life after the anatomical dissection (74% of medical and 41% of premedical students). The most prevalent type of change among male students was "to lead a mechanical way of life" and that among female students was "to be nihilistic". However, there was no change in their religious faith. Furthermore, the change they showed toward human beings was rather more positive. (68% of females vs. 44% of males among premedical students, and 96% of females vs. 74% of males among medical students) Most of students experienced the mysteriousness of human beings and importance of human life. An interesting finding in regard to medical students' personality, 72% of them regarded themselves negatively, such as inhumane, mechanical, or narrow-minded.

0.75Δ% 굴절율차를 가진 40채널 광파장 다중화 및 역다중화 소자 제작 및 특성

문형명,최기선,이길현,김동훈,이지훈,이동환,오진경,곽승찬,권오관,강동수,최준석,정건,이현용,Moon, H.M.,Choi, G.S.,Lee, K.H.,Kim, D.H.,Lee, J.H.,Lee, D.H.,Oh, J.K,Kwak, S.C.,Kwon, O.K.,Kang, D.S.,Choi, J.S.,Jong, G,Lee, H.Y. 한국광학회 2005 한국광학회지 Vol.16 No.3

[ $0.75delta\%$ ]의 평판광회로(PLC;Planar Lightwave Circuit)소자의 설계 및 제작기술을 가지고 저손실과 높은 누화율을 가진 파장 다중화 및 역다중화 소자를 개발하였다. C-band AWG(Arrayed Waveguide Grating)에서의 삽입손실은 2.503이하, 누화율은 35dB이상, 균일도는 1dB이하이며 L-band에서는 Vernier 디자인을 적용하여 ITU-T의 파장 정확도가 0.04nm이하가 되도록 제작하였다. A 40 channel arrayed-waveguide grating (AWG) filter operating in C-band and L-band wavelength regions has been fabricated using PLC (Planar Lightwave Circuit) processes with 0.75 refractive index difference. Its design was optimized for matching the center wavelength with the ITU-recommended wavelength. The characteristics of the fabricated C-band AWG are as follows; average insertion loss < 2.5 dB, polarization-dependent loss < 0.3 dB, non-adjacent crosstalk >35dB, and the loss uniformity of 0.8 dB. In the L-band AWG, wavelength accuracy is below 0.02nm.

인과 칼슘의 수준이 낮은 산란계 사료 내 미생물 Phytase의 첨가가 생산성 및 영양소 소화율에 미치는 영향

민병준,권오석,이원백,손경승,홍종욱,양승주,문태현,김인호,Min B.J.,Kwon O.S.,Lee W.B.,Son K.S.,Hong J.W.,Yang S.J.,Moon T.H.,Kim I.H. 한국가금학회 2005 韓國家禽學會誌 Vol.32 No.1

본 연구는 인과 칼슘의 수준이 낮은 산란계 사료내 미생물 phytase의 첨가가 생산성 및 영양소 소화율에 미치는 영향을 평가하기 위하여 실시되었다. 사양시험은 50주령 ISA brown 갈색계 192수를 공시하였으며, 사양시험은 7일간의 적응기간 후, 12주간 실시하였다 시험설계는 옥수수-대두박 위주의 사료에 NRC 요구량에 맞춰 Available P과 Ca을 처리한 구(CON; 기초사료), 기초사료에 Available P과 Ca을 각각 대조구 사료의 $50\%$와 $90\%$를 함유한 사료에 3종류의 미생물phytase PHYTEX, Agranco. Co. $0.01\%$(P1), Natuphos, BASF $0.06\%$(P2), PHOSMAX GENOFOCUS $0.06\%$(P3)를 첨가한 처리구로 4개 처리를 하였다. 총 12주간의 사양 시험기간동안 산란율은 처리구간에 유의적인 차이를 보이지 않았다 (P>0.05). 시험 종료시 난중은 처리구에서 유의적으로 높게 평가되었다(P<0.05). 난각 두께는 시험 9주째 phytase를 급여한 처리구가 대조구와 비교하여 두꺼워졌다(P<0.05). 시험개시시와 종료시 혈청 내 Ca 함량의 차이는 P1처리구에서 가장 적은 변화를 보였으나, 처리구간 유의적인 차이는 보이지 않았으며, P함량에 있어서도 처리구간 유의성은 보이지 않았다(P>0.05). 경골 내 Ca과 P의 함량은 phytase를 급여한 처리구가 대조구에 비하여 유의적인 차이 없이 높은 경향을 보였다(P>0.05). DM, N과 Ash의 소화율에 있어서는 대조구와 P1 처리구가 P2, P3 처리구와 비교하여 유의적으로 증가하였다(P<0.05). Ca과 P에 있어서는 P2 처리구의 소화율이 가장 높게 나타났으며(P<0.05)), 대조구와 P1 처리구는 서로 통계적인 차이를 보이지 않았다(P>0.05). 모든 처리구들이 대조구보다 유의하게(P<0.01) 낮았고 Ercherichia coli의 수는 유의한 차이가 없었다. Lactobacilli의 수는AM30구가 대조구보다 유의하게(P<0.05) 높았다. 결론적으로 활성 미네랄수 $30\%$흡착 zeolite의 사료 내 첨가는 대조구에 비하여 육계의 증체율을 개선시키는 경향이 있었으며 깔짚에서 암모니아 가스 발생도 유의하게 감소시켰고 장내 Lactobacilli의 수를 유의하게 증가시켰다.결과를 얻을 수 있으리라 생각한다.내산에서는 S.typhimurium $(33\%)$이 분리 되었으나, 미국산 닭날개에서는 Salmonella 속균이 전혀 분리되지 않아 대조적이었다. 하지만, 중국산의 닭부분육(혼합육)에서는 S. enteritidis와 L. monocytogenes도 함께 분리되었다. 그러나 부분육의 경우 국내산 및 수입산 모두에서 Campylobacter 속균과 E. coli O157:H7 균주는 전혀 분리되지 않았다. 서울${\cdot}$경기지역의 재래시장과 백화점에서 시판되고 있는 계란 총 446개에 대해서도 동일한 절차와 방법으로 조사하였던바, 재래시장에서 구입했던 계란의 난각부분(Egg-shell)에서만 가금티푸스(fowl Typhoid)의 병원체인 S. gallinarum이 1주$(0.2\%)$만이 분리되었고, 기타 세균으로서는 대장균군이 역시 난각에서 가장 높은 빈도로 분리되었고, 난황(Yolk)에서는 극히 낮은 수준의 세균오염도를 보였다. 다양한 동물종유래 S. aureus 균주들의 유전학적 분석목적에 가장 신뢰도 높고 감별능력이 뛰어난 분석기법으로 선발되었다.cid로부터 cyclooxygenase를 통해 일단 생성된 endoperoxide에서 각각의 prostaglandin을 생성하는 효소, 특히 $G-Rb_{2}$는 $TxA_{2}$ This Study was conducted to investigate the effects of microbial phytase in low phosphirus and calcium level diet on the performance and nutrient digestibility in laying hens. One hundred ninety two, 50 wks old, ISA brown commerical layers were used for 12 weeks feeding trial after 7-d adjustment period. Four dietary treatments included CON(control; Co.), P2 ($0.06\%$ Natuphos, BASF) and P3 ($0.06\%$ PHOSMAX, GENOFOCUS). Ca and available P concentrations of P1, P2 and P3 were 90 and $50\%$ of NRC recommecdations to accentuate difference in response to phytase availability. In whole period, egg production was not affected by treatments. At 12 weeks, egg weight was significantly increased in adding phytase treatments (P<0.05). Egg shell thickness was increased in P1, P2 and P3 treatments compared with control (P<0.05) at 9 weeks. Ca concentration of serum tended to decrease in P1 treatment without significant difference (P>0.05). Ca and P concentrations of tibia were higher in layers fed dietary phyrase than those fed control diet without significant difference (P>0.05). Digestibilities of DM, N and ash were improved in P1 treatment compared with P2 and P3 treatments (P<0.05). Ca and P digestibilities were the highest in P2 treatment (P>0.05), but was not significant difference between control and P1 treatments.

結核管理資源의 效率的配分에 關한 硏究

文玉綸,朴聖炫,柳元夏,韓達鮮 서울大學校 保健大學院 1979 보건학논집 Vol.16 No.1