Characterization of a novel variant HMW‐glutenin gene from Elymus canadensis

Qian‐Tao Jiang,Yu‐Ming Wei,Tao Liu,Ji‐Rui Wang,Zhi‐En Pu,Xiu‐Jin Lan,You‐Liang Zheng,Zhen‐Xiang Lu 한국유전학회 2010 Genes & Genomics Vol.32 No.4

High molecular weight (HMW) glutenin subunits (GS) play a key role in the determination of end‐use quality of wheat and other cereal crops. In this study, we report the isolation and characterization of both promoter region and ORF of novel HMW‐GS allele 1St1.3 from a perennial Triticeae species,Elymus canadensis. The amino acid (AA) sequences of E. canadensis 1St1.3 were deduced as 434 aa. Its protein primary structure comprises a signal peptide with a conserved N‐terminal domain, a central repetitive domain and a C‐terminal domain. E. canadensis 1St 1.3 possesses several distinct characteristics which are different from those of wheat HMW‐GSs. The N‐terminal domains of E. canadensis 1St 1.3 resemble that of y‐type subunits, while their C‐terminal domains are more similar to x‐type subunits. The deletion of 85 bp fragment has been observed in promoter region of 1St 1.3, however which has not interrupted the expression of this gene. Our results indicate that 1St 1.3 is novel HMW‐GS variants which will be valuable for enhancing our understanding of structural differentiation and the evolutionary relationship among HMW‐GSs in Triticeae species.

Stigma and Efficacy of Zhizhu Kuanzhong Capsules Versus Doxepin in the Treatment of Refractory Functional Dyspepsia: A Randomized Controlled Trial

Qian-Qian Wang,Li Cheng,Bi-Yu Wu,Hong-Yi Qiu,Ping Xu,Bo Wang,Xiu-Juan Yan,Sheng-Liang Chen 대한소화기 기능성질환∙운동학회 2023 Journal of Neurogastroenterology and Motility (JNM Vol.29 No.3

Background/AimsStigma related with antidepressants is prevalent in patients with functional dyspepsia. It affects medication compliance and efficacy. Herbal medicine acquired a deep-rooted cultural identity in relieving dyspeptic symptoms in Asians. The research was designed to compare the effectiveness of Zhizhu Kuanzhong capsules (ZZKZ) versus doxepin hydrochloride (doxepin) on alleviating stigma and medication nonadherence among patients with refractory FD (rFD). MethodsPatients with rFD from February 2021 to February 2022 were randomly allocated to receive either doxepin (n = 56) or ZZKZ (n = 57) combined with omeprazole for 4 weeks. Medication possession ratio (MPR), the disease- and medication-associated stigma were analyzed. The scales were utilized to assess dyspeptic symptoms (Leeds Dyspepsia Questionnaire) and psychological conditions (Generalized Anxiety Disorder Questionnaire and Patient Health Questionnaire). ResultsThe MPR values for ZZKZ were significantly higher than those for doxepin (P < 0.001). The stigma scores decreased in ZZKZ group while increased in doxepin group compared to baseline after treatment. The proportion of patients showing ZZKZ-associated stigma was significantly lower than doxepin-associated stigma (P < 0.001). The MPR values were negatively correlated with post-treatment stigma scores in both groups (P < 0.001). Dyspeptic symptoms and psychological condition were improved in both groups after treatment, with no significant difference on post-treatment Leeds Dyspepsia Questionnaire, Generalized Anxiety Disorder Questionnaire, or Patient Health Questionnaire scores between 2 groups. ConclusionZZKZ is superior to doxepin in alleviating stigma and medication non-adherence, with comparable efficacy in improving dyspeptic symptoms and psychological condition of patients with rFD.

BSA‑carbon dots a promising “off–on” fluorescence probe for detecting glyphosate residues in agricultural products

Qian Wang,Miao Wang,Lufei Zheng,Yongxin She,Jing Wang,Minghong Jia,A. M. Abd El‑Aty 한국탄소학회 2023 Carbon Letters Vol.33 No.7

The widespread and extensive use of glyphosate in agriculture has raised concerns about its potential impact on the quality and safety of agricultural products. Conventional detection methods require long analysis times, making them impractical for the rapid detection of large quantities of samples. Therefore, developing a fast and simple detection system for glyphosate pesticide residues is urgent. In this study, the development of a facile fluorescence probe synthesized using a simple one-pot hydrothermal method for the determination of glyphosate is an important step toward addressing the need for a fast and simple detection system. The present sensor was created using bovine serum albumin (BSA) as a precursor, and the sensor operates by producing an “off–on” fluorescent signal. The bovine albumin-derived BSA-CDs emitted light yellow fluorescence, but this fluorescence was quenched (or suppressed) by the presence of Cu2+ ions. However, the fluorescence can be restored by the presence of glyphosate, which interacts with the Cu2+ ions to form a complex and release the BSACDs from suppression. The functional groups in glyphosate can capture Cu2+ and break the BSA-CDs/Cu2+ combinatorial system. The BSA-CDs/Cu2+ fluorescence quenching system had good selectivity for glyphosate. The detection limit of the BSA-CD/Cu2+ fluorescence sensor was 0.05 μg/mL. This developed method was utilized to successfully detect glyphosate in Chinese wheat. The average recoveries ranged from 98.9 to 100.7%, with a relative standard deviation < 3.0%, showing good prospects for practical applicability.

Adverse effect of DEHP exposure on the serum insulin level of Balb/c mice

Jing Wang,Jinquan Li,Kashif Rafiq Zahid,Kun Wang,Yan Qian,Ping Ma,Shumao Ding,Xu Yang,Xianliang Wang,Xianliang Wang,Xu Yang 대한독성 유전단백체 학회 2016 Molecular & cellular toxicology Vol.12 No.1

Di(2-ethylhexyl) phthalate (DEHP) is a common indoor pollutant in the world, which may cause lots of harmful effects in human including diabetes according to epidemiological studies. To explore the underlying role of DEHP in diabetes-like symptoms, Balb/c mice were chose to be the experimental animals in this paper. They were separated as eight groups as follows: (1) saline+normal diet (vehicle control), (2) 10 mg/kg.day DEHP+normal diet, (3) 50 mg/kg.day DEHP+normal diet, (4) 250 mg/kg.day DEHP+normal diet, (5) streptozotocin (STZ)+high fat diet (diabetes model), (6) 10 mg/kg.day DEHP+ STZ+high fat diet, (7) 50 mg/kg.day DEHP+STZ +high fat diet, (8) 250 mg/kg.day DEHP+STZ+high fat diet. The biomarkers of this experiment include four groups: (1) general indicates: body weight and drinking water, (2) blood biomarkers: serum insulin and fasting glucose, (3) pathological examination: pancreas section and kidney section, and (4) biomarkers of oxidative stress: reactive oxygen species (ROS) and malondialdehyde (MDA) in liver cells. Our study results demonstrate that: (1) at our treatment levels DEHP cannot directly induce diabetes, but reduce serum insulin level in DEHP-exposed non-STZ-treated animals, (2) pathological examination finds that these is a dose-dependent damage in the pancreas in DEHP- exposed STZ-treated groups, and (3) the oxidative mechanism may be involved in this pathological process.

Melatonin protects endothelial progenitor L255cells against AGE-induced apoptosis via autophagy flux stimulation and promotes wound healing in diabetic mice

Haiming Jin,Zengjie Zhang,Chengui Wang,Qian Tang,Jianle Wang,Xueqin Bai,Qingqing Wang,Majid Nisar,Naifeng Tian,Quan Wang,Cong Mao,Xiaolei Zhang,Xiangyang Wang 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Wound healing is delayed in diabetic patients. Increased apoptosis and endothelial progenitor cell (EPC) dysfunction are implicated in delayed diabetic wound healing. Melatonin, a major secretory product of the pineal gland, promotes diabetic wound healing; however, its mechanism of action remains unclear. Here, EPCs were isolated from the bone marrow of mice. Treatment of EPCs with melatonin alleviated advanced glycation end product (AGE)-induced apoptosis and cellular dysfunction. We further examined autophagy flux after melatonin treatment and found increased light chain 3 (LC3) and p62 protein levels in AGE-treated EPCs. However, lysosome-associated membrane protein 2 expression was decreased, indicating that autophagy flux was impaired in EPCs treated with AGEs. We then evaluated autophagy flux after melatonin treatment and found that melatonin increased the LC3 levels, but attenuated the accumulation of p62, suggesting a stimulatory effect of melatonin on autophagy flux. Blockage of autophagy flux by chloroquine partially abolished the protective effects of melatonin, indicating that autophagy flux is involved in the protective effects of melatonin. Furthermore, we found that the AMPK/mTOR signaling pathway is involved in autophagy flux stimulation by melatonin. An in vivo study also illustrated that melatonin treatment ameliorated impaired wound healing in a streptozotocin-induced diabetic wound healing model. Thus, our study shows that melatonin protects EPCs against apoptosis and dysfunction via autophagy flux stimulation and ameliorates impaired wound healing in vivo, providing insight into its mechanism of action in diabetic wound healing.

Study on the combustion process and work capacity of a micro free-piston engine

Qian Wang,Liming Dai,Kai Wu,Jin Bai,Zhixia He 대한기계학회 2015 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.29 No.11

With the main idea of exploring combustion conditions and the work capacity of the micro free-piston engine, the study concern isvisualization work including Homogeneous charge compression ignition (HCCI) combustion in the micro-chamber. The initial freepistonvelocity was adjusted to achieve a wide range of compression ratio. The combustion characteristics, the piston motion and pressurevariations under different compression ratios were discussed. Results indicate that the critical combustion condition occurs when thecompression ratio rises to a certain degree. Two-stage combustion characteristics can be observed in micro HCCI combustion processeswith the fuel of the DME/oxygen mixture. The micro-chamber pressure increases with the increase of the compression ratio. The criticalpeak pressure of 5.4 MPa is obtained when the initial piston velocity reaches 15.0 m/s and the diameter and the length of the microchamberis 3 mm and 37 mm, respectively.

Preparation and Characterization of Acid and Pepsin-soluble Collagens from Scales of Croceine and Redlip Croakers

Qian-Qian Wu,Tao Li,Bin Wang,Guo-Fang Ding 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.6

Acid-soluble (ASC) and pepsin-soluble collagen (PSC) from scales of croceine croaker (ASCPC and PSC-PC) and redlip croaker (ASC-PP and PSC-PP) were prepared with yields of 0.37±0.08% (ASCPC), 1.09±0.23% (PSC-PC), 0.42±0.09% (ASC-PP), and 1.14±0.30% (PSC-PP) (dry scale basis). Gly (347.1- 359.8 residues/1,000 residues) was the major amino acid. Contents of imino acid were between 189.4 and 192.9 residues/1,000 residues in all 4 collagens. ASC-PC and ASC-PP were type I collagens. Differences in subunit components between ASC and PSC were observed. Denaturation temperature values of ASC-PC, ASC-PP, PSC-PC, and PSC-PP were 30.7, 30.1, 27.5, and 26.6℃, respectively. All 4 collagens were soluble at pH 1-4. Values declined when NaCl concentrations exceeded 2%. Freeze-dried collagens showed loose, fibrous, and porous structures. The 4 scale collagens can be alternatives to collagen from terrestrial animals for applications in functional food, cosmetic, and biomedical industries.

“Why-for are you such a horrible contradiction?”: Kipling and the “Chinese Question”

( Qian Wang ) 한국영어영문학회 2019 영어 영문학 Vol.65 No.2

While there have been numerous studies on Rudyard Kipling’s writing about the British Raj, relatively little has been done on his perception of China. This paper looks at Rudyard Kipling’s representation of China in his collection of early travel letters From Sea to Sea and Other Sketches: Letters of Travel (1899). The encounter with Chinese immigrant labourers provoked profound psychological disturbances for Kipling, as was revealed in his persistent and obsessive need to solve the “Chinese question” throughout these letters. This paper analyses the figuration of the Chinese and considers how it contributes to the debate about the effects of Chinese immigration emerging in the second half of the nineteenth century. Kipling’s representation of the immigrant Chinese partakes of, and is conducive to, a Yellow Perilist preoccupation with invasion scares. Further, Kipling’s seemingly unaccountable fear and extreme hatred of the Chinese were the result of his frustration to solve “the Chinese question.” The supposedly paradoxical nature of the Chinese frustrated Kipling’s attempt to interpret them in clear-cut ways and in turn provoked his extreme abhorrence and vituperation. Finally, the paper also reflects on the ways in which different formations of imperialism might inflect British representations of China, as indicated by Kipling’s writing.

Multimode Retrieval of Breast Masses Based on Association Rules

Qian Wang,Yanan Lv,Lixin Song 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.2

Breast imaging case not only image low level features but also has image semantic features. In order to implement multimode retrieval of breast imaging, using feature selection algorithm based on association rules to select features, digging out the associated relationship between image low level features and image semantic features, and then taking advantage of association classification algorithm to get image visual semantic features, which reduced the semantic gap between image low level features and visual semantic features, at last, making similarity measure combined with low level features, to make multimode retrieval come true. As the results show, this method improve the performance of breast imaging case retrieval and provide more meaningful decision support for doctors.

Research on Partial Discharge Pattern Recognition in GIS Based on EFPI Sensor

Wang Zan,Liu Zhongquan,Qiao Lili,Qian Dingdong,Chen Zhongxian,Gao Chaofei,Wang Wei 대한전기학회 2024 Journal of Electrical Engineering & Technology Vol.19 No.1

An EFPI fber optic ultrasonic sensor can be used for the detection and pattern recognition of partial discharge ultrasonic signals in Gas Insulated Switchgear. Compared with traditional piezoelectric sensors, it has many advantages, such as high sensitivity and strong anti-interference ability. Based on this, four typical PD models of the tip, metal particles, suspension and creeping surface were set up in the GIS cavity flled with 0.4 MPa SF6 gas, and the EFPI sensor was innovatively used to detect the discharge ultrasonic signal and extract the single ultrasonic pulse signal. The waveform features form a feature parameter database, and the probabilistic neural network algorithm and the support vector machine algorithm are used for pattern recognition and comparative analysis, respectively. The ultrasonic signal detected by the EFPI sensor has prominent features. On the basis of extracting the feature parameters, the two pattern recognition algorithms can achieve an average recognition rate of more than 85%, and the recognition efect of the support vector machine is better than that of the probabilistic neural network