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Osamu Mikami,Hiroyuki Yamaguchi,Hideo Murata,Yasuyuki Nakajima,Shigeru Miyazaki 대한수의학회 2010 Journal of Veterinary Science Vol.11 No.2
Six 1-month-old piglets were intravenously injected with deoxynivalenol (DON) at the concentration of 1 mg/kg body weight, with three pigs each necropsied at 6 and 24 h post-injection (PI) for investigation of hepatotoxicity and immunotoxicity with special attention to apoptotic changes and cytokine mRNA expression. Histopathological examination of the DON-injected pigs revealed systemic apoptosis of lymphocytes in lymphoid tissues and hepatocytes. Apoptosis of lymphocytes and hepatocytes was confirmed by the TdT-mediated dUTP-biotin nick end-labeling (TUNEL) method and immunohistochemical staining against singlestranded DNA and cleaved caspase-3. The number of TUNELpositive cells in the thymus and Peyer’s patches of the ileum was increased at 24 h PI compared to 6 h PI, but the peak was at 6 h PI in the liver. The mRNA expression of interleukin (IL)-1β, IL-6, IL-18, and tumor necrosis factor (TNF)-α in the spleen, thymus and mesenteric lymph nodes were determined by semi-quantitative RT-PCR, and elevated expression of IL-1β mRNA at 6 h PI and a decrease of IL-18 mRNA at 24 h PI were observed in the spleen. IL-1β and IL-6 mRNA expressions increased significantly at 6 h PI in the thymus, but TNF-α decreased at 6 h PI in the mesenteric lymph nodes. These results show the apoptosis of hepatocytes suggesting the hepatotoxic potential of DON, in addition to an immunotoxic effect on the modulation of proinflammatory cytokine genes in lymphoid organs with extensive apoptosis of lymphocytes induced by acute exposure to DON in pigs.
( Yasuhiro Morita ),( Shigeki Bamba ),( Osamu Inatomi ),( Kenichiro Takahashi ),( Takayuki Imai ),( Masaki Murata ),( Masashi Ohno ),( Masaya Sasaki ),( Tomoyuki Tsujikawa ),( Akira Andoh ) 대한장연구학회 2020 Intestinal Research Vol.18 No.2
Background/Aims: We retrospectively analyzed Crohn’s disease (CD) patients with small intestinal strictures who underwent single-balloon enteroscopy (SBE) to ascertain whether prototype SBEs with a passive bending mechanism and high force transmission insertion tube had better insertability in the small intestine than a conventional SBE. Methods: Among 253 CD patients who underwent SBE, we identified 94 CD patients who had undergone attempted endoscopic balloon dilatation (EBD) for small intestinal stenosis for inclusion in this study. We analyzed whether the type of scope used for their initial procedure affected the cumulative surgery-free rate. For the insertability analysis, patients who underwent SBE at least twice were divided into 3 groups according to the type of scope used: conventional SBE only, prototype SBE only, and both conventional and prototype SBEs. For each group, depth of insertion, procedure time, and number of EBDs were compared in the same patient at different time points. Results: The success rate of EBD was 88.3%. The 5- and 10-year cumulative surgery-free rate was 75.7% and 72.8%, respectively. Cox regression analysis indicated that the factors contributing to surgery were long stricture (≥2 cm), EBD failure, and elevated Crohn’s Disease Activity Index, but not the type of scope used for EBD. The prototype SBEs significantly improved the depth of insertion (P=0.03, Wilcoxon’s signed-rank test). Conclusions: In CD patients with small intestinal stenosis, the prototype SBEs with a passive bending mechanism and high force transmission insertion tube did not improve long-term EBD outcome but did improve deep insertability. (Clinical Trial Registration No. UMIN000037102) (Intest Res 2020;18:229-237)
Effect of His 192 Mutation on the Activity of Alginate Lyase A1-III from Sphingomonas Species A1
YOON, HYE-JIN,CHOI, YONG-JIN,MIYAKE, OSAMU,HASHIMOTO, WATARU,MURATA, KOUSAKU,MIKAMI, BUNZO 한국미생물 · 생명공학회 2001 Journal of microbiology and biotechnology Vol.11 No.1
The alginate lyase A1-Ⅲ gene of Sphingomonas species A1 is composed of 1,077 nucleotides, encoding a protein (359 amino acids) with a molecular mass of 40,322Da. Recombinant A1-Ⅲ expressed in Escherichia coli exhibited the same full enzymatic activity as native A1-Ⅲ. In order to identify the critical residue for activity, a site-directed mutation was introduced into the A1-Ⅲ gene (H192A, His192→Ala). Recombinant A1-Ⅲ (H192A) exhibited a significant decrease in enzyme activity (one-thirty thousandth of that of A1-Ⅲ), without any conformational change, as detected by the CD spectra in the far UV region. Also, the chemical modification of wild-type Al-III with methyl 4-nitro benzene sulfonate resulted in a 40% decrease from the initial activity, whereas the same modification of A1-Ⅲ (H192A) produced no change in the activity. The role of His192 on the catalytic process was also explored based on a model of A1-Ⅲ docked with mannuronic acid into the active site.
사료내 단백원의 차이가 참돔(Pagrus major)과 교잡어 (P. major ♀×Acanthopagrus schlegeli ♂)의 성장과 에너지수지에 미치는 영향
김양수,Biswajit Kumar Biswas,Amal Biswas,지승철,정관식,Osamu Murata,Yoshifumi Sawada,Kenji Takii 전남대학교 수산과학연구소 2013 수산과학연구소논문집 Vol.21 No.-
This study investigated the growth and energy partition of juvenile red sea bream Pagrus major (159.3±9.5 g; RSB) and hybrid sea bream P. major (♀)×Acanthopagrus schlegeli (♂) (144.8± 10.0 g; F1) on satiation feeding of fish meal (FM) and soybean meal (SM) based isocaloric diets (21.1 kJ/g diet) at 21.6℃ water temperature for 4 weeks. Two sea bream species offered no significant influence on final mean body weight, condition factor, viscera somatic index and apparent digestibility coefficient in both dietary groups, and both fish species were increased the relative stomach and intestine weight in SM than that in FM. RSB fed FM diet showed larger growth rate, feed efficiency, protein efficiency ratio, and apparent nutrients and energy retention efficiency than that fed SM diet, but this was inverse in F1. Whereas, Energy partitions of RSB and F1 in FM and SM dietary groups observed a similar tendency on fecal, digestible, urinary, metabolizable and standard metabolizable energy. However, retained energy of RSB in fed FM diet higher than that in SM, was inverse in F1. Moreover, heat increment plus voluntary activity energy consumption of F1 fed SM diet compare with fed FM diet lower than that of RSB. These results suggest that RSB and F1 has similar the energy consumption metabolism for digestive absorption in both diets, however, the energy accumulation pattern from absorbed nutrients appear to differ on SM fed.
Yuji Matsuoka,Kenji Endo,Hidekazu Suzuki,Yasunobu Sawaji,Hirosuke Nishimura,Taichiro Takamatsu,Osamu Kojima,Kazuma Murata,Takeshi Seki,Shinji Horie,Takamitsu Konishi,Kengo Yamamoto 대한척추외과학회 2018 Asian Spine Journal Vol.12 No.4
Study Design: Retrospective study. Purpose: To investigate the relationship between preoperative total spinal sagittal alignment and the early onset of adjacent segment degeneration (ASD) after single-level posterior lumbar interbody fusion (PLIF) in patients with normal sagittal spinal alignment. Overview of Literature: Postoperative early-onset ASD is one of the complications after L4–L5 PLIF, a common surgical procedure for lumbar degenerative disease in patents without severe sagittal imbalance. A better understanding of the preoperative characteristics of total spinal sagittal alignment associated with early-onset ASD could help prevent the condition. Methods: The study included 70 consecutive patients diagnosed with lumbar degenerative disease who underwent single-level L4–L5 PLIF between 2011 and 2015. They were divided into two groups based on the radiographic progression of L3–L4 degeneration after 1-year follow-up: the ASD and the non-ASD (NASD) group. The following radiographic parameters were preoperatively and postoperatively measured: sagittal vertebral axis (SVA), thoracic kyphosis (TK), lumbar lordosis, pelvic tilt, and pelvic incidence (PI). Results: Eight of the 70 patients (11%) experienced ASD after PLIF (three males and five females; age, 64.4±7.7 years). The NASD group comprised 20 males and 42 females (age, 67.7±9.3 years). Six patients of the ASD group showed decreased L3–L4 disc height, one had L3–L4 local kyphosis, and one showed both changes. Preoperative SVA, PI, and TK were significantly smaller in the ASD group than in the NASD group (p<0.05). Conclusions: A preoperative small SVA and TK with small PI were the characteristic alignments for the risk of early-onset ASD in patients without preoperative severe sagittal spinal imbalance undergoing L4–L5 single-level PLIF.
( Yang Su Kim ),( Seung Cheol Ji ),( Amal Biswas ),( Biswajit Kumar Biswas ),( Annita Seok Kian Yong ),( Osamu Takaoka ),( Gwan Sik Jeong ),( Osamu Murata ),( Kenji Takii ) 한국수산과학회(구 한국수산학회) 2014 Fisheries and Aquatic Sciences Vol.17 No.1
To determine a suitable dietary protein/lipid (CP/CL) ratio in the early juvenile stages of hybrid porgy (F1), female red sea bream (RSB) × male black sea bream, five diets with various CP/CL ratios「60/7, 55/12, 51/17, 46/23, and 41/28」were prepared and provided to juveniles in triplicate. At the smaller juvenile stage, F1, weighing 0.32 g, a significantly higher specific growth rate (SGR) and feed efficiency (FE) were seen with 60/7 and 55/12 diets. However, in RSB weighing 0.26 g, SGR and FE were higher with the 60/7 diet than the other diets at 21°C. At the larger juvenile stage, F1, weighing 3.7 g, there was no significant difference in SGR or FE among the diets, but RSB weighing 4.0 g fed 60/7, 55/12, and 51/17 diets had higher SGR and FE than 46/23 and 41/28 diets at 24°C. Moreover, survival and apparent nutrient retention of F1 at both stages were significantly higher than those in RSB. These results indicate that both F1 and RSB weighing ca. 0.3 g require a higher dietary CP/CL than those weighing ca. 4 g. Additionally, F1 in both trials showed the suitability of a lower dietary CP/CL than RSB, indicating that mass production of F1 juveniles will be more economical than RSB.
Effect of Starvation on Survival Rate and Body Composition of
Yang-Su Kim*, Shinj i Yamamoto, Chihiro Aoki, Amal Biswas,Yoshifumi Sawada, Kenj i Takii and Osamu Murata 전남대학교 수산과학연구소 2014 수산과학연구소논문집 Vol.22 No.-
The effect of starvation on Pacific bluefin tuna Thunnus orientalis (PBT) juveniles was investigated according to two stages, weaning stage (0.8±0.2 g) at 25 days post hatching (dph) and transportation stage (3.8±1.2 g) at 36 dph. The juveniles were then transferred (0 h) into each tank from the mass production tank. Starved PBT juveniles were collected from one random tank at 3, 6, 9, 29 and 93 h (25 dph), and 12, 24, 36, 48, 60 and 72 h (36 dph). The survival rate of 25 dph remained above 80% until 78 h: moreover the standard length and weight were increased until 9 h of starvation. However, the survival rate of 36 dph rapidly decreased to 42% at 48 h, and the standard length and weight were gradually decreased until the end of starvation. Whole body lipid and glycogen were rapidly consumed initially and moreover, the protein and energy contents were gradually and steadily decreased during starvation in both experiments. When compared to the linear regression analysis between energy consumption and starvation time, there was a statistical difference between the experiments (P < 0.05). These results suggest that energy mobilization differs between the weaning (25 dph) and transportation stages (36 dph). Further, it is assumed that the juveniles are more sensitive to starvation at 25 dph.
교잡어, 참돔 (Pagrus major, ♀) × 감성돔 (Acanthopagrus schregeli, ♂)의 성장 및 수온변화와 저염분 환경에서의 내성
김양수 ( Yang Su Kim ),지승철 ( Seung Cheol Ji ),( Biswajit Kumar Biswas ),( Amal Biswas ),정관식 ( Gwan Sik Jeong ),( Osamu Murata ),( Ken Ji Takii ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.3
The growth and tolerance for water temperature and salinity were compared among red sea bream Pagrus major (RSB) black sea bream Acanthopagrus schregeli (BSB) and their hybrid (F1), female RSB × male BSB. The growth of the F1 fish did not differ until 27 days after hatching (dAH), after which the most rapid growth was observed until approximately 300 dAH, followed by RSB and BSB. However, the RSB had out grown the F1 fish by approximately 303 dAH. By 480 dAH, the RSB were largest, followed by the F1 and BSB groups. The tolerances for high and low water temperature were significantly different for each species and growth stage. The largest tolerance spectrum was observed in the BSB group, and the tolerance spectrums gradually decreased with increasing final body weight. During the salinity tolerance trials, all of the species started to die following transfer into freshwater (0 psu). BSB showed the highest survival rate when kept in fresh water for an average of 29.9 hours, while the F1 fish were more tolerant than the RSB fish. The fish were increasingly tolerant to fresh water and changes in water temperature as they grew, while the size of the temperature spectrum remained unchanged but shifted to a lower temperature range with growth. Our results demonstrate that F1 performed well in terms of growth compared to parental fish, with higher temperature and salinity tolerances than RSB, and is thus suggested to be a suitable aquaculture species for Korea and northeast China.