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Pseudomonas maltophilia N246의 Alkane Hydroxylation System 관련 유전자들의 분자적 클로닝
황문옥,이나리,민경희 숙명여자대학교 자연과학연구소 1993 자연과학논문집 Vol.- No.4
Pseudomonas maltophilia N246의 OCT plasmid로부터 alkane hydroxylase 유전자를 클로닝하였다. 삽입하는 DNA 크기를 최소화하기 위하여 pUC19의 HindIII site에 subcloning 한 결과 약 4.1 kb의 DNA 절편을 얻을 수 있었다. 제한효소 EcoRI, HindIII, BamHI, PstI, XbaI, SmaI, AccI, PvuII, EcoRV를 이용하여 이 DNA 절편의 제한효소 지도를 작성하였다. E. coli 내에서의 이 유전자의 발현을 조사한 결과 형질전환주들은 alkane hydroxylase 유전자들을 포함하고 있음을 알 수 있었다. Genes coding for alkane hydroxylase, the first step of n-alkane hydroxylation, from OCT plasmid in Pseudomonas maltophilia N246 were cloned in E. coli JM101. The cloned DNA was subcloned to be 4.1 kb DNA fragment in HindIII site of pUC19. This DNA fragment containing the essential genes of alkane hydroxylase was mapped with restriction enzymes EcoRI, HindIII, BamHI, PstI, XbaI, SmaI, AccI, PvuII and EcoRV. These gene expression in transformant E. coli JM101 was examined on the bases of growth on minimal plate containing n-alkane and indicated that transformants had alkane hydroxylase genes.
RBL-2H3 세포에서 IgE-dependent Histamine-releasing Factor의 탈인산화 효소에 관한 연구
황선옥,이경림 이화여자대학교 약학연구소 2005 藥學硏究論文集 Vol.- No.15
IgE-dependent histamine0releasing factor(HRF) was initially described as a secretagogue for secretion of histamine from IgE+basophils from a subset of allergic donors. Previously, we identified that S98 residue of HRF was phosphorylated using anti-HRFpS98 antibody which specifically recognizes the phosphorylated serine residue of HRF and HRFS98A mutant construct. In vitro kinase assay, only wild type HRF was phosphorylated by PKC, and S98A HRF was not affected by PKC. in this study, we attempted to characterize the phosphatase which specifically dephosphorylates HRF by immunoprecipitation and pull-down assay. In RBL-2H3 cells, HRF interacted only with calcineurin (also called as PP2B, calcium/calmodulin-dependent phosphatase ) but not with PP1 or PP2A. The results suggest that HRF is most likely dephosphory lated by calcineurin.
초등학생의 컴퓨터사용 실태에 관한 연구 : 5,6학년을 중심으로
황경애,조명옥 진주산업대학교 2001 論文集 Vol.40 No.-
The purpose of this research was to find the attitudes related in computer use of the elementary school students. The subjects were computer use attitude of 597 elementary school students of 5,6grade in Chinju. The method was self-reporting questionnaire. For data analysis, frequency distribution, percentage, Cronbach's α coefficient were estimated. ANOVA was used in SPSS WIN. The major findings of the research are as follows ; 1. rank of brother and sister ; 1st, last one 2. living family composition ; parent, brother or sister, they(nuclear family) 3. age of parent ; mother(-40), father(44) 4. education level of parent ; highschool graduation, university graduation 5. job of parent ; father(office man), mother(fulltime housewife, commercial work) 6. family economical living level, student's education result ; medium level 7. level of familial intimacy ; intimacy, normal, very intimacy 8. place of computer ; their room, livingroom 9. contents of computer use ; game or entertainment, internet, E-mail 10. most like contents of computer use ; game or entertainment, music, culture, interest, E-mail 11. time of computer use ; randomly, through night 12. amount of computer use in one day ; in one or two hours 13. frequency of internet & game ; everyday, required time 14. which one game together ; brother or sister, by oneself 15. place of computer ; their home 16. desirable computer use ; using time plan, use when required information 17. image of computer ; convenient instrument , information communication use 18. Most of elementary school students had very positive computer/ internet use attitudes in 16items/17. 19. Parental attitudes and mediation was very important in the result of this study. 20. Many parents have to learn computer use method.
ARABON과 CARBON 섬유 여과포의 코팅에 의한 내열성 향상에 관한 연구
이경옥,Kanaoka, Chikao,황우현,박영옥 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.6
고온에서 집진 성능이 우수하고 저렴하게 사용할 수 있는 합성섬유 여과포의 코팅기술을 개발하기 위한 내열성 코팅 실험을 수행하였다. 코팅된 여과포의 내열실험을 300℃ 및 400℃에서 수행한 후, 여파포의 무게변화, 두께변화, 수축률을 측정하여, 내열 특성을 측정하였다. 가열 전후의 무게 변화를 측정한 결과 ARABON 여과포는 300℃에서 36.3% 감소하였고, CARBON 여과포는 38.9%로 감소하였으며, 수축현상은 ARABON이 13.7%로 약간 변화되었지만 CARBON 여과포는 21.7%로 조금 더 변화되었다. 고온에서 합성섬유 여과포의 내열성능을 나타내기 위하여 변화지수, CI를 정의하여 사용하였다. 300℃와 400℃에서 CARBON 여과포의 가장 우수한 코팅물질은 Al(NO₂)₃이었다. This study has been accomplished by the coating experiments to develope the synthetic fabrics with thermal resistance, which are the economic and efficient filter at high temperature. The thermal resistance of the fabric filter was experimentally determined by the changes of the weight, thickness, and contraction of the coated and non-coated filters. The tested temperatures were 300℃ and 400℃. The weight losses of the ARABON and CARBON fabrics are 36.3% and 38.9%, and the contractions are 13.7% and 21.7% at 300℃ compared to 400℃ respectively. The change index, Cl, was defined and used for providing the thermal resistant information of the tested fabrics. The most coating material is Al(NO₂)₃ for CARBON fabrics at 300℃ and 400℃.
OCT 플라스미드를 갖는 원유 분해세균에 의한 Octane 분해능
최순영,김창숙,이명혜,황문옥,민경희 숙명여자대학교 자연과학연구소 1991 자연과학논문집 Vol.- No.2
원유 분해세균에 의한 원유 분해능을 조사한 결과, Xanthomonas campestris M12. Xanthomonas sp. M28, Acinetobacter lwoffi G1, and Klebsiella pneumoniae L25, 그리고 Pseudomonas maltophilia N246 등의 순서로 나타났다. Xanthomonas campestris M12, Xanthomonas sp. M28, 그리고 Pseudomonas maltophilia N246 균주 모두 octane 분해시의 온도는 30℃에서 최적이었으며, 최적 pH는 X. campestris M12와 Xanthomonas sp. M28이 7.0∼7.5이었고, P. maltophilia N246이 7.5∼9.0이었다. N246 균주의 최적 NaCl 농도는 3.0∼3.5%이었다. P. maltophilia N246와 X. campestris M12는 모두 플라스미드를 갖고 있음을 확인하였고, N246 균주로부터 플라스미드를 제거하였을 경우 octane 분해능이 소실되었으므로 이 플라스미드 위에 octane 분해 유전자가 있음이 확인되었다. 이 균주의 OCT 플라스미드의 크기는 118kb이었다. 또한, N246 균주는 ampicillin 항생제에 내성을 나타내었다. Xanthomonas campestris M12. Xanthomonas sp. M28. Acinetobacter lwoffi G1, and Klebsiella pneumoniae L25, Pseudomonas maltophilia N246 were screened to increase the ability for crude oil utilization. All of these could utilize hexadecane and octane with the exception of N 246 strain for only octane biodegradation. Thus N246, M12, and M28, strains were specially examined for octane oxidation. Octane biodegradation by three strains showed the optimal conditions at 30C, pH7.0∼9.0 and 0.2∼0.3% octane concentration as a substrate. It was found that P. maltophila N246 and X. campestris M12 had plasmid and the cured plasmid from N246 strain lost octane utilization. Therefore, it was confirmed that certain genes for octane utilization were located on OCT plasmid in N246 strain. The size of OCT plasmid in N246 strain was 118 kb. The N246 strain was resistant to ampicillin.
김영민,오동렬,이원재,김형국,황두영,이환,최경호,정시경,김세경,김영옥 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.4
Rhabdomyolysis is a biochemical and clinical syndrome caused by lysis of skeletal muscle cell and release of muscle cell contents into the circulation. In addition to traumatic cause, various nontraumatic causes have been reported. Rhabdomyolysis has frequently been associated with seamy aspects of life and society: alcoholic abuse, drug addiction, sadistic drill exercise, war events and attempted suicide. The abuse of alcohol causes many hazards in many organs. One of these is acute alcoholic myopathy that may present as a fulminent syndrome of muscle pain and tenderness associated with rhabdomyolysis and acute renal failure. Because the prognosis of adequately treated rhabdomyolysis is excellent, early recognition and prompt management are essential in emergency situation. We experienced two typical cases of alcohol-induced rhabdomyolysis with acute renal failure and report these cases with literature review.