Case Report : Multiple Osteochondritis Dissecans of Knee Joint in a Patient with Wilson Disease, Focusing on Magnetic Resonance Findings

( Noh Hyuck Park ),( Hyoung Soo Kim ),( Sang Yeop Yi ),( Byung Cho Min ) 대한슬관절학회 2013 대한슬관절학회지 Vol.25 No.4

A 17-year-old man was admitted with a complaint of knee pain. He was diagnosed with Wilson disease by ophthalmologic and laboratory studies during hospitalization. Initial plain radiography of both knees showed multiple osteochondritis dissecans (OCD) on the medial and lateral femoral condyles of both knees. Subsequent magnetic resonance imaging showed multiple OCDs, which were symmetric on both knees. Subchondral cysts on the medial condyle and trochlear dysplasia were additionally evident on both femurs. We report this case with a focus on the imaging findings.

Reliability of MRI Findings of Peroneal Tendinopathy in Patients with Lateral Chronic Ankle Instability

Hee Jin Park,Seung Doh Cha,Hyung Soo Kim,Soo Tae Chung,Noh Hyuck Park,Jeong Hyun Yoo,Jai Hyung Park,Joo Hak Kim,Tae Woo Lee,Chang Hyun Lee,Se Man Oh 대한정형외과학회 2010 Clinics in Orthopedic Surgery Vol.2 No.4

Background: Patients with chronic lateral ankle instability also have peroneal tendinopathy often. However, preoperative MRIs of these patients are vague in many cases. Our study was performed to see the reliability of MRI findings of peroneal tendinopathy in patients with chronic lateral ankle instability. Methods: MRI images for 82 patients who had chronic lateral ankle instability, and had received surgical treatment between March 2006 and November 2009 were compared with impressions from operating rooms. The mean age of patients was 36.4 years (range, 15 to 64 years), 82 ankles were studied, and patients with rheumatoid diseases were excluded from the study. Results: Of the 82 cases, 26 were true positives, 38 true negatives, 13 false positives and 5 false negatives. Of 39 cases of peroneal tendinopathy diagnosed from MRI, 14 had peroneal tendon partial tears, 15 tenosynovitis, 3 dislocations, 17 low-lying muscle bellies, and 6 peroneus quartus muscles. Of 31 cases of peroneal tendinopathy observed in surgery 11 had peroneal tendon partial tears, 4 tenosynovitis, 5 dislocations, 12 low-lying muscle belliess, and 1 peroneus quartus muscle. Sensitivity and specificity of peroneal tendinopathy were 83.9% and 74.5%, respectively. Positive predictive value was 66.7%. Negative predictive value was 88.4%. Accuracy rate was 78.0%. Conclusions: MRI is a useful diagnostic tool for detecting peroneal tendinopathy in patients with chronic lateral ankle instability. However, MRI is vague in many cases. Therefore, a thorough delicate physical examination and careful observation is needed.

XPS STUDY ON THE EFFECT OF LOW-ENERGY ELECTRON IRRADIATION ON DNA DAMAGE BY Fe³⁺ ION

Noh, Hyung-Ah,Park, Yeun-Soo,Cho, Hyuck The Korean Association for Radiation Protection 2015 방사선방어학회지 Vol.40 No.2

We have employed X-ray photoelectron spectroscopy (XPS) technique to examine the combined effects of low-energy electron (LEE) irradiation and $Fe^{3+}$ ion on DNA damage. pBR322 plasmid DNA extracted from E. coli ER2420 was used for preparing DNA-$Fe^{3+}$ sample. The C1s XPS spectra were scanned for LEE-irradiated and LEE-unirradiated samples and then curve-fitted. For the samples with LEE irradiation only or with Fe ion only, no significant changes from pure DNA samples were observed - a single effect of either $Fe^{3+}$ ion or LEE irradiation did not cause a significant damage. However, when these two components were combined, the DNA damage was increased quite significantly, compared to the sum of DNA damages caused by $Fe^{3+}$ ion and by LEE irradiation independently. This observation is consistent with our previous results [Radiat. Res. 177, 775 (2012)] which was done using gel-electrophoresis technique. Partial interpretation of the observed spectrum peaks was also attempted.

X선과 저에너지 전자선에 의한 DNA 손상

Park, Yeun-Soo,Noh, Hyung-Ah,Cho, Hyuck,Dumont, Ariane,Ptasinska, Sylwia,Bass, Andrew D.,Sanche, Leon 대한방사선방어학회 2008 방사선방어학회지 Vol.33 No.2

X선과 같은 고에너지 방사선에 의한 DNA 손상 중 간접적인 손상을 확인하기 위하여 탄탈륨(Ta) 박막위에 동결건조 과정으로 만들어진 pGEM-3Zf(-) plasmid DNA 단일층(monolayer)의 박막을 만든 다음, 에너지가 1.5 keV인 Al $K{\alpha}$ X선을 0분, 3분, 7분, 10분 동안 초고진공 상태에서 이 DNA 단일층에 조사하여 평균 흡수선량(mean absorbed dose)의 변화에 따른 DNA 손상을 관찰하였다. 또한 3 eV의 낮은 에너지 전자선을 조사하여 그 결과를 X선을 조사한 경우와 비교하였다. X선과 낮은 에너지 전자선으로 조사된 plasmid DNA를 전기영동(electrophoresis) 방법을 이용해 supercoiled DNA와 unsupercoiled DNA로 분리한 후 각각을 정량적으로 분석하였다. Supercoiled DNA는 X선과 3 eV 전자선의 조사에 따른 평균흡수선량이 증가함에 따라 선형적으로 감소했다. 그와 반대로 circular DNA와 crosslinked form 1 DNA는 평균흡수선량이 증가함에 따라 선형적으로 증가했다. 이것은 supercoiled DNA가 낮은 에너지 전자와 상호작용하여 외가닥 절단(single strand break)을 일으켰고 그 결과 unsupercoiled DNA로 변화되었음을 보여준다. 본 실험을 통해 X선과 같은 고에너지 방사선에 의한 DNA의 간접적 손상이 일어남을 관찰할 수 있었고, DNA의 이온화 에너지보다 작은 에너지($0{\sim}10\;eV$)를 갖는 전자에 의해서도 DNA 손상이 일어날 수 있음을 확인할 수 있었다. We observed DNA damages as a function of mean absorbed dose to identify the indirect effect of high-energy radiation such as x-ray. Monolayer films of lyophilized pGEM-3Zf(-) plasmid DNA deposited on tantalum foils were exposed to Al $K{\alpha}$ X-ray (1.5 keV) for 0, 3, 7 and 10 min, respectively, in a condition of ultrahigh vacuum state. We compared DNA damages by X-ray irradiation with those by 3 eV electron irradiation. X-ray photons produced low-energy electrons (mainly below 20 eV) from the tantalum foils and DNA damage was induced chiefly by these electrons. For electron beam irradiation, DNA damage was directly caused by 3 eV electrons. Irradiated DNA was analyzed by agarose gel electrophoresis and quantified by ImagaQuant program. The quantities of remained supercoiled DNA after irradiation were linearly decreased as a function of mean absorbed dose. On the other hand, the yields of nicked circular (single strand break, SSB) and interduplex crosslinked form 1 DNA were linearly increased as a function of mean absorbed dose. From this study, it was confirmed that DNA damage was also induced by low energy electrons ($0{\sim}10\;eV$) even below threshold energies for the ionization of DNA.

Effect of low-energy electron irradiation on DNA damage by Fe3+ ion.

Park, Yeunsoo,Noh, Hyung Ah,Cho, Hyuck Academic Press 2012 Radiation research Vol.177 No.6

<P>We investigated the combined effects of low-energy electron irradiation and Fe(3+) ion on DNA damage. We used lyophilized pBR322 plasmid DNA films with various concentrations (0 ~ 7 mM) of Fe(3+) ions and irradiation with monochromatic, low-energy 3 or 5 eV electrons for these studies. DNA-Fe(3+) films were recovered and analyzed by agarose gel electrophoresis to identify and compare the effects of Fe(3+) ions and/or low-energy electrons alone or in combination on DNA damage. In nonirradiated DNA-Fe(3+) films, there was little DNA damage observed (less than 10% of the total DNA loaded on the gel appeared damaged) for Fe(3+) ion up to 7 mM concentration. In irradiated DNA films without Fe(3+) ions, there was also very little DNA damage observed (less than 3% of the total DNA loaded on the gel appeared damaged). However, when DNA-Fe(3+) films, were irradiated with low-energy electrons, DNA damage was significantly increased compared to the sum of the damage caused both by either Fe(3+) ion or low-energy electrons irradiation alone. We proposed that both DEA and/or electron transfer processes might play a role in the enhanced DNA damage when DNA-Fe(3+) films were irradiated by low-energy electrons.</P>

Effect of Low-Energy Electron Irradiation on DNA Damage by Cu²⁺ Ion

Noh, Hyung-Ah,Park, Yeunsoo,Cho, Hyuck The Korean Association for Radiation Protection 2017 방사선방어학회지 Vol.42 No.1

Background: The combined effect of the low energy electron (LEE) irradiation and $Cu^{2+}$ ion on DNA damage was investigated. Materials and Methods: Lyophilized pBR322 plasmid DNA films with various concentrations (1-15 mM) of $Cu^{2+}$ ion were independently irradiated by monochromatic LEEs with 5 eV. The types of DNA damage, single strand break (SSB) and double strand break (DSB), were separated and quantified by gel electrophoresis. Results and Discussion: Without electron irradiation, DNA damage was slightly increased with increasing Cu ion concentration via Fenton reaction. LEE-induced DNA damage, with no Cu ion, was only 6.6% via dissociative electron attachment (DEA) process. However, DNA damage was significantly increased through the combined effect of LEE-irradiation and Cu ion, except around 9 mM Cu ion. The possible pathways of DNA damage for each of these different cases were suggested. Conclusion: The combined effect of LEE-irradiation and Cu ion is likely to cause increasing dissociation after elevated transient negative ion state, resulting in the enhanced DNA damage. For the decrease of DNA damage at around 9-mM Cu ion, it is assumed to be related to the structural stabilization due to DNA inter- and intra-crosslinks via Cu ion.

유방촬영술에서 악성이 의심되는 미세석회화의 초음파 발견율과 특징적인 소견

박노혁,김미성,송순영,이은자,박찬섭 關東大學校 醫科大學 醫科學硏究所 2003 關東醫大學術誌 Vol.7 No.2

To evaluate the US detectability and findings of suspicious microcalcification detected on mammography. fifteen patients with mammographically detected microcalcifications clusters and 10 patients with segmental distributed microcalcifications prospectively underwent US with a 10- or 14- MHz transducer within 1 month interval. And we analyze the US detectability and findings of suspicious microcalcification on mammopaphy respectively. We detect 12 cases of 15 mammographically clustered microcalcification on US. Of these,3 cases are located in the mass with distinct margin and 7 cases are seen as echogenic reflectors in the patchy hypoechoic background with indistinct margin.2 cases are in the irregularly dilated duct. Also we detect 6 cases of 10 mammographically segmental distributed microcalcifications on US. Of these, 2 cases are located in the mass and 4 cases are in the patchy hypoechoic background with indistinct margin. Given a known mammographic location, US with high-frequency transducer can depict suspicious microcalcification at high detection rate, which is seen as echogenic reflectors in the mass, patchy hypoechoic background or irregularly dilated duct.

The role of metal ions on low energy electron-induced biomolecular alteration

Yeunsoo Park,Hyung Ah Noh,Hyuck Cho 한국진공학회 2017 한국진공학회 학술발표회초록집 Vol.2017 No.2

실차시험을 이용한 팬터그래프의 소음기여도 분석

오혁근(Hyuck Keun Oh),노희민(Hee-Min Noh),김준곤(Jun-Kon Kim),박춘수(Choonsoo Park) 한국철도학회 2016 한국철도학회논문집 Vol.19 No.3

The XPS Analysis of DNA Damage Induced by Low Energy Electron Collision and New Design of Separated Nonthermal Plasma Source

Yeunsoo Park,Hyung Ah Noh,Hyuck Cho 한국진공학회 2015 한국진공학회 학술발표회초록집 Vol.2015 No.2