월출산국립공원 수질특성에 관한 연구

최형일,김난희,강영주,채희정,김연풍,조도호,설동화 조선대학교 환경공해연구소 2007 環境公害硏究 Vol.20 No.1

Because mountain streams have narrow widths and steep slopes compared to streams and lakes, hours of immobility are considerably short, and the quality of mountain streams has been greatly influenced by rainfall, land-use, components of the streambed and types of forests. During the summer when heavy rainfall occurs, eroded earth and sand flow into mountain streams and cause temporarily high turbidity. It is also the cause of eutrophication, which is frequently found in downstream. As evaluation standards for the quality of mountain streams were not established in Korea, this quality was evaluated by examining some items based on quality standards for drinkable water and research on the influences of pollution in mountain streams, which showed that these were seldom in downstream water systems. Mountain streams within Wolchulsan National Park flow into a reservoir and when characteristics of mountain streams from forests and rainfall that flow into mountain streams through forest ecosystems were studied, results of measuring its changes are as follows. Monthly changes of pH were low between April and August and higher starting October, and the EC was measured higher in the winter and the spring and tended to be low starting April. The EC on the 19th of May and the 23rd of October and the concentration of floating materials and COD_(Mn) were observed to be higher due to the influence of rainfall on the 19th of May and the 22nd of October, and it was judged that the washing out of dried deposits attached to the water pipe and the leaching of woods were influenced by rainfall. It is considered that real-time monitoring of water quality for a long time and water quality conditions during and after rainfall should be examined for ensuring basic data of water quality and evaluating characteristics and seasonableness of mountain streams, which may be greatly changed by artificial influence in the future.

철도교통소음의 수평·수직적 전달 및 감쇠 특성

최형일,정원삼,김난희,강영주,위환 조선대학교 환경공해연구소 2006 環境公害硏究 Vol.19 No.1

This study has been conducted to achieve the following objectives: First, in order to understand the horizontal propagation and attenuation characteristics for the railroad traffic noise, we selected actively developed areas within 100 meters away from the road to measure the level of traffic noise near a road. To measure the noise near a railroad, we selected areas within 100 meters away from the railroad and then selected Saemaul-ho and Mugoongwha-ho as the subjects for our experiment, In this way, we analyzed the horizontal propagation and attenuation characteristics for the traffic noise occurring in diversified areas. Second, in order to understand the vertical propagation and attenuation characteristics for the railroad traffic noise, we measured and analyzed the distributional characteristics of vertical sound pressure levels on each floor of multi-storied apartment buildings according to changes of traffic load and types, and the existence or nonexistence of soundproof walls. For the case of the railroad traffic noise, we also selected Samaul-ho and Mugoongwha-ho as the subjects for our experiment, and we measured and analyzed the different noise levels on each floor of multi-storied apartment buildings from the soundproof wall. The results of Horizontal propagation and attenuation characteristics for the railroad traffic noise are as follows: In cases of the flat land, cutting land, and bridge area, as distance increases, the sound pressure level steadily decreases. The cutting land has an especially high distance decrease compared to the other areas, and the sound pressure level for the bridge area is higher than that of the flat land with a measurement of 9.7~17.5㏈(A). Vertical propagation and attenuation characteristics for the railroad traffic noise are as follows: The amount of sound pressure level decrease is 13.9~15.4㏈(A) for Samaul-ho and 10.9~11.5㏈(A) for Mugoongwha-ho when measuring the vertical sound pressure levels at heights lower than 4.5m, which indicates a fairly large decrease. At 6m, the amount of decrease is 7.3㏈(A) for Samaul-ho and 5.0㏈(A) for Mugoongwha-ho, which indicates a small decrease.

뇌경색 환자에서의 혈장 지질과 항산화 영양소 상태에 관한 연구

최영선,이난희,서정규,박의현,조성희 경북대학교 병원 1998 경북대학교병원의학연구소논문집 Vol.2 No.1

뇌경색 환자의 생활 습관, 혈장 지질 및 적혈구 지질 및 적혈구 지질의 지방산 조성과 혈장 지질과산화물 농도, 항산화영양소인 비타민 E, A, β-carotene 상태를 측정하여 대조군의 자료화 비교함으로써 뇌경색 환자의 혈장 지질과 항산화영양소 상태를 조사하였다. 18명의 남자 환자군의 평균연령은 65.7(45~76)세였으며 36명의 건강한 남자대조군의 평균연령은 59.2(55~65)세였으며, 체중, 신장 및 BMI는 대조군과 환자군 간에 유의한 차이가 없었다. 환자군의 발병전 흡연 정도와 음주정도는 대조군보다 유의하게 높았으며, 적혈구지방산 조성에 있어서 포화지방산의 비가 유의하게 높았고 다불포화지방산의 비는 유의하게 낮았으며, 통계적인 유의성은 없으나 항산화 영양소 상태가 대조군에 비하여 불량하고 혈장 과산화지질의 종도가 높은 경향을 보였다. 이의 원인으로서는 환자군이 대조군에 비해 흡연율이 높고 음주량이 많으며 운동부족 등 부적합한 생활습관이 주요한 요인으로 작용한 것으로 판단되며, 따라서 바람직한 생활습관이 뇌경색질환의 예방에 기본요건이 된다고 사료된다. To investigate antioxidant and lipid staus of patients with cerebral infarction, 18 male patients admitted to Kyungpook National University Hospital from March to October, 1995 were compared to 36 healty male control subjects. The percentages of heavy smoking, heavy drinking and nonexercise before diagnosis of the disease in the patient group were significantly higher compared to the current status of the control group. Total cholesterol, LDL cholesterol and lipoprotein(a) were significantly higher in the patients than the control subjects. For the fatty acid composition of erythrocytes, total saturated fatty acids(% by weight) were significantly higher and total polyunsaturated fatty acids lower in the patient group than in the control group. Plasma levels of retinol, α-tocopherol and β-carotene were not significantly different between the patient group and the control group at the level of P<0.05 Alpha-tocopherol/total cholesterol of plasma was lower in the patient group with the statistical significance of P=0.054, and the plasma level of TBARS of the patient group was 1.98 nmol MDA/mL as compared to the level of the control, 1.60 nmol MDA/mL(P=0.066). Our results indicate that oxidative stress was increased in the patients with cerebral infraction, and such outcome might have resulted from undesirable life styles.

사격장 소음에 관한 연구

최형일,정경훈,정원삼,박싱일,김난희 조선대학교 환경공해연구소 2006 環境公害硏究 Vol.19 No.1

These days, the way of life and peoples' values have been changing in a developing society. The noise pollution in residential areas is a serious problem caused by the drift of population to cities, increased density, and heavy traffic. The firing ranges, which were built 20 or 30 years ago, are required facilities but are threatening to people. They are a source of anxiety as they jeopardize the residents' safety and destroy their living. Because of this, problems arise often. To reduce the problems between the residents and the military, the sound level around the firing range, the location of the range, and training times are investigated as the following 1. When a howitwer is fired, if there is no obstacle, at distances of 70m and 120m, the equivalent noise level is +116㏈(A)Leq and 98.6㏈(A)Leq, respectively. When the distance is 120m, the maximum sound pressure level is 123㏈(A)Leq for a period of one second. 2. When a 105mm howitwer is fired, if there is a hill as a natural sound barrier and the distance between the firing location and the target location is far more than 900m. the equivalent sound level is below 62㏈(A)Leq. 3. Upon firing, the sound pressure levels have equal range. The sound level is higher at 500Hz and lOOOHz. 4. The sound pressure level of the noise of explosive sound decreases between the shooting point and the target point. At distances of lOOOm, 1400m, and 1450m, the equivalent sound levels are 86.2㏈(A)Leq, 85.4㏈(A)Leq and 84.2㏈(A)Leq each, and the highest sound pressure levels are 121.4㏈(A)Leq, 116㏈(A)Leq and 114.3㏈(A)Leq for one second. 5. The explosive sound at the target point is similar to the noise at the firing point. The sound pressure level also has an equal range. Also. the sound level is higher at 500Hz and 1OOOHz than the others.

서울 서남부지역 노인 인구에서 비만증 유병률과 고지혈증과의 상관성

최경묵,조영직,김경오,김동림,김난희,백세현,안정천,서홍석,오동주,최동섭 대한노인병학회 2000 Annals of geriatric medicine and research Vol.4 No.3

Lipopolysaccharide 로 유발된 생쥐 무릎관절낭 염증에 관한 형태학적 연구 : 윤활관절막과 섬유관절막의 변화를 중심으로 Based on the Morphological Changes of Synovial Membrane and Fibrous Membrane

김진택,안상현,최난희,정재만,박인식,강윤호,김호현,이해풍 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.7 No.2

본 연구는 관절염 유발시 일어나는 관절낭의 형태학적 변화를 조사하기위해 ljpopolysaccharide(LPS)주사로 인위적 관절낭 염증을 유발시킨 후 시간경과에 따른 윤활관절막과 섬유관절막의 형태 변화를 관찰하였다. BALB/C 암컷 생쥐 오른쪽 무릎관절낭에 LPS 300㎍/㎏를 주사한 후 3, 7 그리고 14일에 무릎관절을 얻었다. 무릎관절은 4주동안 EDTA용액에 탈회한 후 통상적 방법으로 paraffin에 포매하였다. 또한 윤활관절막의 미세구조변화는 embed812로 포매한 후 관찰하였다. LPS 주사후 관절연골 인접부위의 윤활관절막에서 시작된 세포과형성(hyperplasia)은 시간 경과후 전체 윤활관절막으로 확대되었다. 윤활관절막내의 미세구조의 변화로는 윤활포식세포(type 1)가 관절강내로의 많은 돌기(filopodia)를 내었고, 잘 발달된 과립형질내세망을 가지는 type 2 윤활분비세포의 숫적 증가가 보였다. 한편 LPS 주사후 섬유관절막에서 나타나는 형태학적 변화는 collagen fiber 생성에 의한 섬유화가 증가되며, 이러한 섬유화를 주도하는 섬유모세포의 이주증가파 관찰되었다. 또한 혈관 주위에서는 백혈구의 이주 증가가 나타났으며, 탈과립형(degranulated type) 비만세포가 많이 관찰되었다. 이상의 결과로 LPS 주사로 관절낭에서 염증이 유발되어 윤활관전막과 섬유관절막에서 형태학적 변화가 나타났다. 이러한 일련의 형태학적 변화는 발병초기 류마티스성 관절염에서 나타나는 병리학적 소건과 동일한 결과로서, 앞으로 진행될 치료제 개발과 유발기전에 관한 해석을 위한 in vivo 실험의 적절한 모델로 기여한 것으로 기대된다. Synovial joint of BALB/C mice were injeced with Lipopolysaccharide(LPS) were observed to investigate the morphological changes of synovial capsule caused by rheumatoid arthritis(RA). The RA on female Balb/c mice were induced by LPS injection, as dose of 300㎍/㎏, into synovial cavity of knee joint. And then these specimen were fixed in 10% neutral buffered formalin and were decalcificated in EDTA solution for 4 weeks. The hyperplasia of synovium were appeared in synovial membrane. The filopodia of phagocytic like synoviocyte(type Ⅰ synoviocyte) projected into synovial cavity and the number of fibroblast like synoviocyte(type Ⅱ synoviocyte) with well-developed endoplasmic reticulum were increased in synovium. In fibrous membrane, the fibrosis induced by synthesis of collagen fiber were enlarged to all fibrous membrane, and the number of fibroblast were increased. A great number of inflammation component cell as Iymphocyte and neutrophil leukocyte were infiltrated around capillary and the degranulate typed mast cell were increased. As results indicated that the hyperplasia of synovium induced by LPS, subsequently to cause the fibrosis, infiltration of imflammation component cell, and increase of degranulated type mast call as same as symptoms of RA.

Triton WR-1339 주사에 의한 고지혈증 유발 생쥐 신장에 미치는 참홑파래 추출물의 지방축적 억제 영향

박인식,안상현,최난희,김진택 동국대학교 한의학연구소 1998 東國韓醫學硏究所論文集 Vol.6 No.2

본 실험은 참흩파래 추출물의 항고지혈증 효과의 조사를 위해 시행된 것으로 ICR 생쥐에 Triton WR-1339(TX) 복강주사로 고지혈증을 유발시킨 후 참흩파래 추출물(30mg/kg)를 복강주사하여 시간의 경과에 따른 신장조직의 일반적인 형태변화와 콜레스테롤을 비롯한 지방입자 축적양상 변화를 관찰하였다. TX 주사 후 신장조직에서 나타나는 일반적인 형태변화는 콩팥소체의 혈관극과 요세관극 모두에서 단층편평상피인 보우만주머니의 외벽이 비후되어 단층입방상피의 형태로 바뀌었으며 요세관 공간도 확장되었다. 지방입자는 사구체 전지역, 근위곱슬세관, 원위곱슬세관 그리고 헨레고리에서 침착이 증가된 것으로 나타났으며, 지방입자의 크기도 증가하였다. 또한 콜레스테롤 입자도 전 지역에서 월등하게 증가한 분포양상을 보였다. 그러나 TX 주사 후 참흩파래 추출물 처리군에서는 보우만주머니 외벽세포의 비후와 요세관공간의 확장을 일부 콩팥소체에서만 관찰할 수 있었으며, 대부분은 정상적인 신장형태를 유지하고 있는 것으로 나타났다. 한편 지방입자는 TX 주사군에 비해 그 분포와 크기가 감소한 것 나타났으며, 또한 콜레스테롤입자도 일부 지역에서 나타나 그 분포가 감소된 것으로 나타났다. 이상의 결과로 볼 때 참흩파래 추출물은 TX 주사로 고지혈증이 유발된 생쥐에 대해 콜레스테롤을 비롯한 지방축적을 억제하는 항고지혈증 효과를 하는 것으로 사료된다. Renal tissues of ICR mouse were intraperitoneally injeced with Monostroma nitidum extract(ME) after Triton WR-1339(TX) injection were observed to investigate the lipid accumulation suppressive effect of ME. The renal tissues were obtained at hour 24, 48, and 72 after TX injection that were fixed in fromol-calcium solution and were cryocut. The tissue stained by H&E for general morphology, sudan black B for lipid and perchloric acid-naphthoquinone method for cholesterol. The parietal layer of Bowman's capsule is swelled as cuboidal type at hour 48 after TX injection and the lipid blot and cholesterol particle were noticeably increased in glomerulus than control of group and these appeared in convoluted tubule, collecting tubule, and Helen's loop. But the parietal layer of Bowman's capsule was shaped as squamous type that was looked like as normal morphology in ME treated group after TX injection and the lipld blot and cholestero1 particle were considerably decreased in glomerulus than TX group. As results indicated that the accumulation of lipid including cholesterol caused by TX injection were mitigated in renal tissues by the antihyperlipidermic effect of ME.

Cyclosporin A 로 유도된 생쥐 림프절의 세포성 면역억제에 관한 면역조직화학적 연구 : T 림프구, IL-2 수용기 및 NK세포의 변화를 중심으로 Based on the change of T lymphocytes, Il-2 receptors, and NK cells

김진택,박인식,안상현,최난희,김동환 동국대학교 한의학연구소 1998 東國韓醫學硏究所論文集 Vol.6 No.2

본 실험은 cyclosporin A(CsA)에 의한 시간의 경과에 따른 림프절에서의 세포성 면역억제를 조사하기 위해서 시행된 것으로 BALB/C계 생쥐에 10일동안 CsA(45mg/kg/day) 투여 후 림프절에서의 T 림프구, IL-2 수용기 그리고 자연살해(NK)세포의 분포 변화를 관찰하였다. 대조군의 림프절에서는 L3T4(CD4)에 양성반응을 보이는 도움 T림프구, Ly2(CD8)에 양성반응을 보이는 세포독성 T 림프구 그리고 CD25R에 양성반응을 보이는 IL-2 수용기를 가진 세포는 곁피질(paracortex)과 수질동(medullary sinus)에서 분포하였다. CsA투여 후 처음 3일까지는 이들 양성반웅세포의 분포 변화는 없었으며 양성반응성의 변화도 없었다. 그러나 CsA 투여 7일부터 양성반응 세포수의 감소와 양성반응성의 약화가 관찰되기 시작하였으며 이러한 변화는 시간이 경과하여 14일에 이르렀을 때 가장 큰 감소추세로 나타났다. 한편 NKl.1(CD56)에 양성반응을 보이는 자연살해세포는 피질과 수질에 분포하였으며 CsA 투여 후 시간의 경과에 따라 양성반응 세포수가 감소하였으며, 이러한 감소는 14일에서 가장 큰 것으로 나타났다. 이상의 결과로 미루어보아 CsA 투여는 림프절에서의 IL-2 분비저해를 통해 T 림프구와 NK세포의 활성을 차단하여 선택적이면서 효과적인 세포성 면역억제작용을 하고 있는 것으로 사료된다. Cyclosporin A(CsA) is a selective immunosuppressive agent that has been credited with improved survival of solid organ allografts. Lymph node of BALB/C mouse administered CsA immunohistochemically observed to understand immunosuppressive effects of CsA on T lymphocytes, IL-2 receptors, and natural killer NK cells in lymph node. CsA orally administered daily for 10days at the dose 4smg/kg/day/. The lymph node were obtained at day 3, 7, and 14 after CsA administration and embeded with paraffin, and then stained by following ABC method that used monoclonal antibody including L3T4(CD4), Ly2(CD8), IL-2R(CD25), and NK-1.1(CD56). There were little changes of reactive degree and number of helper T lymphocytes, cytotoxic T lymphocytes, IL.-2 receptors, and NK cells at day 3 after CsA administration, but they began to decrease at day 7. These decrease were greatest at day 14. The helper T lymphocytes, cytotoxic T lymphocytes, IL-2 receptors, and NK cells distributed in paracortex and medullary sinus. These results indicated that the secretion of IL-2 began to decrease at day 7 after CsA administration and subsequently to suppress T lymphocytes and NK cell as components of cell-mediated immunity.

금호강수계 수질과 하천유역의 토양 및 농작물의 중금속루 오염에 관한 연구(II)

金相烈,李萬正,趙康烈,崔閏壽,石敬順,禹美姬,李蘭姬 대구효성 가톨릭 대학교 1984 연구논문집 Vol.29 No.2

Soil are mostly sandy and it has a little clay below 10%. In the fine sand fraction, quartz, plagioclase and alkali-feldspar are dominant in almost all samples. Small amounts of altered-feldspar, muscovite, biotite, hornblende and plant opal are present in some samples. They are mainly derived from weathering prodructs df granite, granodiorite and arkose or is mixture. The clay minerals are mainly expanding or nonexpanding 14A˚minerals, illite and kaolin minerals. Nonexpanding 14A˚minerals seems to be dioctahedral vermicurite which sandwiches gibbsite or chlorite.

Serum Insulin , Proinsulin and Proinsulin / Insulin Ratio in Type 2 Diabetic Patients : As an Index of β - Cell Function or Insulin Resistance

Nan Hee Kim,Dong Lim Kim,Kyung Mook Choi,Sei Hyun Baik,Dong Seop Choi 대한내과학회 2000 The Korean Journal of Internal Medicine Vol.15 No.3

Background : Although insulin resistance and decreased insulin secretion are characteristics of establised type 2 DM, which of these metabolic abnormalities is the primary determinant of type 2 DM is controversial. It is also not well known how insulin res