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Chung, Namhyun 한국응용생명화학회 2001 Journal of Applied Biological Chemistry (J. Appl. Vol.44 No.4
Contaminated sites are often abandoned for an extended period of time, thus organopollutants becoming sequestered. The information on the initial concentrations of the contaminants would be helpful for the economic bioremediation of the chemicals. The present study estimated the initial concentrations of atrazine and phenanthrene through multiple regression analyses using soil properties and the amount of chemicals available in situ. Percentage mineralized or extracted was best correlated with organic C or logarithm of organic C, and the $R^2$ values were 0.548 and 0.894 for atrazine and phenanthrene, respectively. Estimation of the initial concentration of the chemicals was then calculated from both the percentage mineralized or extracted and the amount of chemicals extracted or mineralized. Results showed that the estimation of the initial concentration of the chemical at the time of contamination is feasible.
Chung, Eunsu,Gao, Rongke,Ko, Juhui,Choi, Namhyun,Lim, Dong Woo,Lee, Eun Kyu,Chang, Soo-Ik,Choo, Jaebum Royal Society of Chemistry 2013 Lab on a chip Vol.13 No.2
<P>We report the rapid and highly sensitive trace analysis of mercury(ii) ions in water using a surface-enhanced Raman scattering (SERS)-based microdroplet sensor. Aptamer-modified Au/Ag core-shell nanoparticles have been fabricated and utilized as highly functional sensing probes. All detection processes for the reaction between mercury(II) ions and aptamer-modified nanoparticles were performed in a specially designed microdroplet channel. Small water droplets that included sample reagents were separated from each other by an oil phase that continuously flowed along the channel. This two-phase liquid-liquid segmented flow system prevented the adsorption of aggregated colloids to the channel walls due to localized reagents within encapsulated droplets. The result was reduced residence time distributions. The limit of detection (LOD) of mercury(II) ions in water was determined by the SERS-based microdroplet sensor to be below 10 pM, which is three orders below the EPA-defined maximum contaminant level. This combination of a SERS-based microfluidic sensor with aptamer-based functional nanoprobes can be used for in-the-field sensing platforms, due to its size and simplicity.</P>
( Ji Young Lee ),( Namhyun Chung ),( Yong Kwon Lee ) 한국응용생명화학회(구 한국농화학회) 2014 Journal of Applied Biological Chemistry (J. Appl. Vol.57 No.1
Aged black garlic (ABG) was extracted with 20% ethanol and water (crude extracts) and fractionated into three categories (>10, 3-10, and <3 kDa). The effect of crude extract supplements on anti-My-10 hybridoma cell growth and IgG1 antibody production was investigated in suspension culture with a chemically defined protein-free medium. We observed that supplementation of ABG to the cell culture medium stimulated anti-My-10 hybridoma cell growth and production of IgG1 antibody, particularly with fractionated ABG of low molecular weight. The stimulation depended upon the concentration and the size of the fractionated ABG. We also found that the growth promoting activity was not correlated with high antibody production. These results suggest that fractionated ABG is a novel and promising alternative as an animal cell culture supplement.
Woo, Mi-Hee,Kim, Min Soo,Chung, Namhyun,Kim, Joong-Su The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.5
A codon-optimized 2-deoxyribose-5-phosphate aldolase (DERA) gene from Haemophilus influenzae Rd KW20 was synthesized and expressed in Escherichia coli, and the biochemical properties of its product were investigated. DERA was purified using affinity chromatography and characterized using 2-deoxyribose-5-phosphate as the substrate. Specific activity of the recombinant DERA was $34.1Umg^{-1}$. The pH and temperature optima were 7.5 and $40^{\circ}C$, respectively. Additionally, the recombinant enzyme retained stability up to temperatures below $50^{\circ}C$. Maximal enzyme activity was observed in presence of 300 mM of acetaldehyde. The apparent $K_m$ and $V_{max}$ of purified enzyme towards 2-deoxyribose-5-phosphate were 0.14 mM and $70.42{\mu}mol\;min^{-1}mg^{-1}$ and towards 2-deoxy-D-ribose were 24.77 mM and $1.94{\mu}mol\;min^{-1}mg^{-1}$, respectively. For synthesis of statin intermediates, the bioconversion process for production of (3R, 5S)-6-chloro-2,4,6-trideoxy-erythro-hexose from chloroacetaldehyde and acetaldehyde using the recombinant DERA was studied and this process took 3 h for maximal conversion. This recombinant DERA could be potentially applied in the production of (3R, 5S)-6-chloro-2,4,6-trideoxy-erythro-hexose.