Cloning, Purification, and Characterization of a New DNA Polymerase from a Hyperthermophilic Archaeon, Thermococcus sp. NA1

( Yun Jae Kim ),( Hyun Sook Lee ),( Seung Seob Bae ),( Jeong Ho Jeon ),( Jae Kyu Lim ),( Yo Na Cho ),( Ki Hoon Nam ),( Sung Gyun Kang ),( Sang Jin Kim ),( Suk Take Kwon ),( Jung Hyun Lee ) 한국미생물생명공학회 2007 Journal of microbiology and biotechnology Vol.17 No.7

Hierarchically nanoporous pyropolymer nanofibers for surface-induced sodium-ion storage

Yoon, Hyeon Ji,Lee, Min Eui,Kim, Na Rae,Yang, Seung Jae,Jin, Hyoung-Joon,Yun, Young Soo Pergamon Press 2017 Electrochimica Acta Vol. No.

<P><B>Abstract</B></P> <P>Surface-driven charge storage materials based on both electrochemical double layer (EDL) formation and pseudocapacitive behavior can deliver high energy and power capabilities with long-lasting cycling performance. On the other hand, the electrochemical performance is strongly dependent on the material properties, requiring sophisticated electrode design with a high active surface area and a large number of redox-active sites. In this study, hierarchically nanoporous pyropolymer nanofibers (HN-PNFs) were fabricated from electrospun polyacrylonitrile nanofibers by simple heating with KOH. The HN-PNFs have a hierarchically nanoporous structure and an exceptionally high specific surface area of 3,950.7m<SUP>2</SUP> g<SUP>−1</SUP> as well as numerous redox-active heteroatoms (C/O and C/N ratio of 10.6 and 16.8, respectively). These unique material properties of HN-PNFs resulted in high reversible Na-ion capacity of ∼292mAhg<SUP>−1</SUP> as well as rapid kinetics and stable cycling performance in the cathodic potential range (1-4.5V vs. Na<SUP>+</SUP>/Na). Furthermore, energy storage devices based on HN-PNFs showed a remarkably high specific energy of ∼258 Wh kg<SUP>−1</SUP> at ∼245Wkg<SUP>−1</SUP> as well as a high specific power of ∼21,500Wkg<SUP>−1</SUP> at ∼78 Wh kg<SUP>−1</SUP>, with long and stable cycling behaviors over 2,000 cycles.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Hierarchically nanoporous pyropolymer nanofibers (HN-PNFs) were fabricated. </LI> <LI> HN-PNFs have a high specific surface area of ∼3,950m<SUP>2</SUP> g<SUP>−1</SUP> and numerous heteroatoms. </LI> <LI> HN-PNFs exhibit high capacities of ∼292mAhg<SUP>−1</SUP> and great rate/cycling performances. </LI> <LI> High energy (258Whkg<SUP>−1</SUP>) and high power (21,500Wkg<SUP>−1</SUP>) are achieved. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Hierarchically nanoporous pyropolymer nanofibers (HN-PNFs) fabricated from electrospun polyacrylonitrile nanofibers by simple heating with KOH exhibited high electrochemical performance for Na-ion storage.</P> <P>[DISPLAY OMISSION]</P>

스트릿 댄스 시장의 과제와 개선 방안 연구 : 전문가 인터뷰를 중심으로

이우재, 나윤빈,문병순 한국실용무용학회 2024 한국실용무용학회지 Vol.2 No.1

본 연구는 최근 미디어 등을 통해 주목받고 있는 스트릿 댄스의 주요 과제를 파악하고 이에 대한 개선방안을 모색하였다. 문헌조사와 더불어 관련 종사자 겸 전문가 10명을 대상으로 FGI를 실시하였다. 연구 결과, 국내 스트릿 댄스 시장은 아직까지 제도적 미비함이 많은 것으로 나타났고, 이를 계약, 일자리, 법제도 측면으로 나누어 보았을 때, 일자리 정보 공유, 의무적인 표준계약서 사용,법적 갈등에 대한 지원 등의 개선방안이 필요한 것으로 나타났다. 아울러 향후 지속가능한 형태의 발전을 위한 방안으로는 스트릿댄스의 허브 역할을 할 공적 협회의 설립 및 운영이 필요하다. This study looked at street dance, which has recently become popular through the media. The method was to identify major problems in the market and find ways to improve them. A literature survey was conducted, and then focus group interviews were conducted with 10 related workers and experts. As a result of the study, the domestic street dance market still had many institutional insufficiencies. This was divided by type into contracts, jobs, and legal system aspects. Here, improvement measures such as sharing job information, andatory use of standard contracts, and support for legal conflicts were found to be necessary. In addition, for future mid- to long-term evelopment, it is necessary to establish and operate a public association that will serve as a hub for street dance.

건설 사업장의 작업 공정별 분진 발생량 평가

장봉기,이종화,박종안,최윤나,이익진 순천향대학교 기초과학연구소 2004 순천향자연과학연구 논문집 Vol.10 No.2

Airborne dust emission among construction industry place of work that locate on Pyeongtaek, Gyeonggi province of 4 processes (a plaster, cutting, hammer drill, concrete grinding) were analyzed. Work did in state that a worker does not exist except measurer as well as is not attained to control group. Investigation period did for 2 months from October to November. It measured 15 times by each process by personal air sampling method that is direct-reading particulate matter measurement device(digital dust indicator). When a plaster work, the dust was class 3 particulate matter, and geometric mean is 2.14㎎/m³, and occasion that exceed 10㎎/m³ that is threshold limit value was 13.3%. Control group was 0.35㎎/m³. plaster work was statisically difference higher than the control group (p<0.001). It was not significant difference between fixed type cutting machine work (0.96㎎/m³) and control group. It were not exceeded for threshold limit value. Geometric mean was 10.44㎎/m³ at movable type cutting machine process. Threshold limit value of this process was exceeded 53% and concentration of total dust was significantly higher than control group (p<0.001). Geometric mean was 3.22㎎/m³ at diameter 13.45㎜ drill work among hammer drill work and all cases not exceeded for threshold limit value, but it was significantly higher than control group(p<0.001). When work diameter 6.5㎜ drill, geometric mean was 4.06㎎/m³ and exceeded for threshold limit value was 13.3%, it was significantly higher than that of control group (p<0.001). It showed higher concentration of dust by diameter is smaller drilling. All 15cases exceeded threshold limit value at concrete grinding work, geometric mean was 78.38㎎/m³, and it was significantly higher than that of control group (p<0.001). As a result of above, countermeasure preparation that concentration of total dust by each process in construction workplace was specially required because exceeded threshold limit value of working environment more than 50% at process of concrete grinding work and movable type cutting machine.

피질하 혈관성 치매 환자에서 협착성 뇌혈관 병변의 의의 : 자기공명 혈관조영술과 양전자방출단층촬영을 이용한 연구 MR Angiography and ^(18)FDG-PET Study

유경호,이병철,마효일,김윤중,정지향,나덕렬,강연욱 대한치매학회 2004 Dementia and Neurocognitive Disorders Vol.3 No.1

Background and Objective:Subcortical vascular dementia (SVD) incorporates small vessel disease as the primary vascular etiology, represents in MRI by lacunar infarct and deep white matter lesions. In clinical practice, a number of SVD patients present with various vascular risk factors for large artery disease (LAD) i.e., old age, hypertension, and diabetes. However the current diagnostic criteria for SVD did not include angiographic findings as a key feature. Therefore we tried to find out the frequency of large artery disease in SVD and to elucidate whether the presence of LAD affects clinico-radiological manifestations of SVD. Methods:Thirty three patients fulfilling 'Research criteria for SVD' by Erkinjuntti from 17 study centers were recruited. Of these 33 patients, 21 who underwent comprehensive neuropsychological test battery, MRI ^(18)FDG-PET, and MR angiography were classified into two subgroups according to the patterns of MRI abnormality:white matter dominant (WM) type (n=5) and multiple lacunar (LC) type (n=16). Clinical and neuropsychological profile and ^(18)FDG-PET findings of 21 SVD patients with and without LAD were compared. Results: Twelve (57%) of 21 SVD patients had mild stenotic lesions in intracranial large arteries:4 in middle cerebral artery, 8 in posterior cerebral artery, and 4 in vertebrobasilarj artery. There were no differences in vascular risk factors, characteristics of cognitive dysfunctions, and ^(18)FDG-PET findings between SVD with and without LAD. Conclusions:Our results showed that the presence of mild degree of large artery stenosis did not affect the clinical., neuropsychological profile and cerebral metabolism in PET. Therefore, the current 'Research criteria for SVaD' by Erkinjuntti could be used to diagnose SVD as a homogenous clinical group regardless of angiographic abnormalities.

일개 대학병원 산업의학과 외래환자의 질병분포와 내원 경로에 관한 분석

김상훈,이지나,임종한,홍윤철 大韓産業醫學會 2002 대한직업환경의학회지 Vol.14 No.4

목적 : 본연구는 산업의학과의 발전과 영역확장을 위한 중요한 요소인 산업의학과 외래진료를 시행하고 있는 일개 대학병원 산업의학과 내원 환자의 질병 분포와 내원 유형을 알아보기 위해 실시되었다. 방법 : 인천지역 일개 대학병원 산업의학과에서 외래 진료를 받은 1,682명에 대해 전산 및 수기 의무기록 자료를 통해 성, 연령, 질병의 분포 및 외래 방문경로를 조사하였다. 결과 : 1998년부터 2001년까지의 4년간 진료건수는 5,157건, 환자는 1,682명이며, 고엽제 클리닉 환자를 제외하면 839명의 혼자를 진료하였다. 839명의 환자에 대한 분석결과는 다음과 같다. 1. 성별로는 남자 654명(77.9%), 여자 185명 (22.1%)이며, 연령분포는 40대가 가장 많았다(남자 중 31.7%, 여자 중 34.7%). 2. 연도별로는 1998년 146명, 1999년 174명, 2000년 247명, 2001년 272명으로 증가하고 있다. 방문경로는 '검진' 548명(65.3%), '대행' 132명(15.7%), '스스로방문' 125명(14.9%), '타원전원' 18명(2.2%), '타과전원' 16명(1.9%) 이며, '검진'이 전체에서 차지하는 비중은 낮아지고, '스스로방문', '대행'은 높아지고 있다. 3. 질환별로는 간장질환이 241명(28.7%), 호흡기질환 107명(12.8%), 위장관질한 86명(10.3%), 근골격계질환 73명(8.7%)이었으며, '검진'에서 많은 간장질환, 당뇨, 빈혈의 비중은 낮아지고, '스스로방문'과 '대행'에서 많은 근골격계 질환의 비중은 높아지고 있다. 결론 : 외래진료 운영 초기에는 '검진'을 통한 일차이료적인 외래진료가 많았지만 점차 '스스로방문' 및 '대행'을 통한 산업의학 고유영역에 해당되는 환자가 증가하고 있다. 외래 운영 초기 안정을 위해서는 '검진'등을 통한 일차진료를 위주로 하며, 안정된 후에는 기본적으로 일차진료를 수행하면서 산업의학과의 전문적 영역을 확보해 나간다면 지속적인 산업의학과 외래진료의 발전 및 성장이 가능할 것이다. Objectives: The occupational & environmental mecicine clinic plays an important role in the growth and advace of occupational & environmental medicine. This study analyzes the disease distribution and referral routes of patients in an occupational & environmental medicine clinic of a university hospital. Methods : 843 Agent Orange cases and 839 other cases weretreated in the clinic over a period of 4 years. The Agent Orange cases were excluded from this study. Distributions of age, sex, disease and referral route were examined according to the medical records of the hospital. Results: 654 of the patients inthe study population were male(77.9 %) and 185 were female(22.1 %). The most common age group consisted of patients in their forties. The numer of cases has been increasing anually(1998, 146cases: 1999, 174 cases: 2000, 247 cases: 2002, 272 cases). The source of the cases were as follows: health examination. 548(65.4%): health management service, 132(15.7%): self-referral, 125(14.9 %): physicians in other hospitals, clinics, 18(2.1 %): and physicians in other clinic in the hospital, 16(1.9 %). The most commonly occurring disease was liver disease(28.6 %), followed by respiratory disease(12.8 %), gastroenteric disease(10.3 %) and musculoskeletal disease(8.7 %). When the clinic was first opened, most cases came through 'health examination'. Subsequently the number of cases arsing from 'self-refferal' and 'health management services' were increased. The disease distribution has also changed as the referral route distribution evolved. Conclustions: In order to expand the work of the clinic, primary cares for patients who came from 'health examination' will be needed to settle down the clinic at the beginning. Thereafter, specialized services need to vbe developed for cases originating from 'self-referral' and from referral by other organizations. Increasing the number of xases needs to go hand in hand with the development of good management skills.

출퇴근 소요시간이 남자 근로자의 혈중 Gamma-glutamyltransferase에 미치는 영향

김상훈,이지나,홍윤철 大韓産業醫學會 2002 대한직업환경의학회지 Vol.14 No.4

목적: 음주, 비만, 운동부족 등 체내에 산소성 손상(oxidative stress)을 유발하는 인장들은 산소성 손상(oxidative stress)의 기전을 통해 간세포내 단백질 손상을 주어 혈중 γ-GTP를 상승시키는 것으로 알려져 있다. 출퇴근 소요시간의 증가는 운동량 감소를 초래하기 때문에, 출퇴근 소요시간의 증가가 혈중 γ-GTP 상승을 유발할 것으로 가설을 설정하고 이를 확인하고자 하였다. 방법: 근무지가 김포공항에서 인천공항으로 옮겨지고, 직무 및 근무형태가 변하지 않은 5 개 회사남자 근로자 717명을 대상으로 하여 김포공항 근무 당시 시행한 체격검사, 혈액검사 결과 근무지 이전 전후의 출퇴근 소요시간, 수면시간, 음주량 설문 결과를 이용하여 출퇴근 소요시간이 혈중 γ-GTP에 미치는 영향을 분석하였다. 결과: 공항 이주전 평균 출퇴근 소요시간은 62.86분이었으며, 이주 후에는 139.30 분으로 유의한 출퇴근 소요시간 증가가 있었다. γ-GTP와 유의한 상관관계를 보이는 변수들로는 출퇴근 소요시간(r=0.11), 체질량지수(r=0.27), 소축기혈압(r=0.18), 이완기혈압(r=0.24), 혈색소(r=0.12), 혈당(r=0.08), 총콜레스크롤(r=0.24), AST(r=0.49), ALT(r=0.49), 음주량(r=0.10)이 있었다. γ-GTP를 종족변수로 하여 다른 변수들과의 다변량 회귀분석을 시행한 결과, γ-GTP느 출퇴근 소요시간, 체질량지수, 이완기혈압, 총콜레스테롤, AST, ALT, 음주량과 유의한 연관성을 나타내었으며, 출퇴근 소요시간이 60분 이상일 경우 출퇴근 소요시간과 γ-GTP 간에 양-반응관계가 나타났다. 공항이주 전인 2000년당시 정상 γ-GTP값을 나타내었던 685명의 남자 근로자에 대하여 출퇴근 소요시간 변화량이 60분이하, 60 분초과로 구분한 후, 공항이주 후인 2002년 γ-GTP값이 정상/비정상 군으로 구분하여 비교위험도를 구한결과 출퇴근 소요시간이 60 분이하 증가기간 군보다 60 분 초과 증가한 군에서 γ-GTP값이 비정상으로 될 비교위험도는 2.20(95% CI : 1.14-4.23 )로 나타났으며, 기준을 90분, 120 분으로 구분하면 각각 2.43(95% CI : 1.34-4.38), 2.57(95% CI : 1.36-4.86)로 비교위험도가 증가하였다. 결론: 이 결과는 남자 근로자에서 출퇴근 소요시간의 증가가 운동부족을 초래하며, 헐중 γ-GTP 상승를 일으키는 인자임을 시사한다. Objectives: Alcohol drinking, obesity, and reduced physical activity are commonly related to oxidative stress, which induces plasma membrame-bound protein damage in hepatic cells and elevates serum γ-GTP. We have a hupothesis that a long commuting time may induce oxidative stress. This study was undertaken to investigate the relationship between commuting rime and serum γ-GTP level. Methods: Questionnaires were administered to 717 male workers who had worked at Kimpo airport until the year 2000, and had then moved to Incheon airport and were working there in 2002. The questionnaire included items concerning commuting time, sleeping time and alcohol consumption. Phusical examination and laboratoru data were also collected. Results: Serum γ-GTP level was significantly and positively correlated with commuting time, bodu mass index, systolic blood pressure,, diastolic blood pressure, hemoglobin, glucose, total cholesterol, AST, ALT and alcohol consumption. Multivariate regression analysis demonstrated that serum γ-GTP level is significantly associated with commuting time, after considering the other coviates. Conclusions: Commuting time is one of the factors that affects serum γ-GTP level in male workers.

Piezoelectric Properties of Lead-Free Na_0.5K_0.5NbO₃ Ceramics Co-Doped with NiO and CuO

Tai, Weon-Pil,Kim, Young-Hyeok,Ok, Yun-Po,Ji, Han-Na,Kang, Sang-Kyu,Lee, Jae-Shin Informa UK (Taylor Francis) 2010 Integrated ferroelectrics Vol.114 No.1

색상 측정 기기를 이용한 복합레진 적층 수복과 단일 수복의 색상 비교 분석

송영상,김자현,이빈나,장지현,장훈상,황윤찬,오원만,황인남 大韓齒科保存學會 2012 Restorative Dentistry & Endodontics Vol.37 No.2

Objectives: This study analyzed the difference in color caused by different thickness in enamel layer of composite resins when applied with single and layering placement technique, and evaluated if the results agreed with the shade guide from the manufacturers to verify reliability of the color matching process of the manufacturers. Materials and Methods: For single composite resin samples, 6 mm diameter and 4 mm thickness cylindrical samples were fabricated using Ceram-X mono (DENTSPLY DeTrey) and CIE L*a*b* values were measured with spectrophotometer. Same process was done for layering compositie resin samples, making 3 dentinal shade samples, 4 mm thickness, for each shade using Ceram-X duo (DENTSPLY DeTrey) and enamel shade resins were layered in 2 mm thickness and CIE L*a*b* values were measured. These samples were ground to 0.2 mm thickness each time, and CIE L*a*b* values were measured to 1 mm thickness of enamel shade resin. Results: Color difference (△E*) between single and layering composite resin was 1.37 minimum and 10.53 maximum when layering thicknesses were between 1 mm and 2 mm and 6 out of 10 same shade groups suggested by manufacturer showed remarkable color difference at anythickness (△E* > 3.3). Conclusion: When using Ceram-X mono and duo for composite resin restoration, following the manufacturer's instructions for chossing the shade is not appropriate, and more accurate information for Ceram-X duo is needed on the variation and expression of the shades depending on the thickness of the enamel. (Restor Dent Endod 2012;37(2):84-89)

볼리비아 로즈목에 의한 다형홍반 양상을 보인 직업성 알레르기 접촉피부염 증례

신주연,홍윤철,임종한,박신구,이지나,김환철 大韓産業醫學會 2004 대한직업환경의학회지 Vol.16 No.2

배경 : 로즈목(rosewoods) 중 볼리비아 로즈목(Machaerium Scleroxyon)은 강력한 감작물질을 함유하고 있어서 외국에서는 이로 인한 자극 또는 알레르기 접촉피부염의 발생이 증가하고 있음에도 불구하고 지금까지 우리나라에서는 볼리비아 로즈목을 비롯한 수입목재에 의한 직업성 피부질환의 보고가 부족하였고, 이에 저자들이 볼리비아 로즈목에 의한 다형홍반 양상을 보인 직업성 알레르기 접촉피부염의 증례를 경험하였기에 보고하고자 한다. 증례 : 49세 남자로서 여러 가지 수입목재를 절단하고, 다듬는 작업을 하다가 소양감을 동반한 홍반성의 피부 증상이 발현되었고, 전신으로 증상부위가 확대되어 2주 동안 병원 입원 치료를 하게 되었으며, 이후 본원 산업의학과에서 피부첩포 검사 후 최종적으로 볼리비아 로즈목에 의한 알레르기 접촉피부염으로 확진되었다. 결론 : 수입목재들로 인한 직업성 피부질환 발생에 대한 광범위한 실테 조사가 필요하며, 이를 토대로 작업장에서의 직업성 피부질환의 발생을 감소시키기 위한 다각적인 예방, 관리 대책의 수립이 필요하다. Background: Woods are capable of causing allergic or irritant contact dermatitis which typically occurs on exposed areas. Because Bolivian rosewood exclusively contains the strong sensitizer R-3,4-dimethoxdalbergione, an increasing number of cases of initant and allergic contact dermatitis has been reported. Case report: A 49-year-old, male, wood worker, who handled a variety of woods, developed allergic contact dermatitis. A patch test confirmed a positive reaction to one of the exotic woods, Bolivian rosewood. Allergens found in Bolivian rosewood caused this patient's dermatitis, which cleared when he avoided &is wood. Conclusion: Exotic woods such as Bolivian rosewood can induce occupaiional contact dermatitis. Measures to lower the incidence of work-related dermatitis are needed in the workplace. Especially, when cutting or sanding woods, workers should wear proper personal protection.