응고제가 우유두부의 물성 및 관능적 특성에 미치는 영향

우나리야,이민선,박수진,강명화 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.5

Different types of coagulant and characteristics of its concentration added in the process of making milk curd were compared in this study. The pH of whey decreased when the amount of coagulant increased. The turbidity of whey was decreased when 5% of acetic acid and lemon juice were put into as coagulants. The texture of milk curd made with cow's milk and skim milk was measured the highest when acetic acid and lemon juice were added at 3, 5% of their concentration. The fracturability of milk curd made only with cow's milk decreased as the concentration of each coagulant increased. The cohesiveness was decreased as concentration of coagulant increased. The springiness was slightly changed depending on its coagulant but didn't show much of difference. The gummminess of milk curd made with cow's milk was increased when 3, 5% of coagulant was added. The result of sensory evaluation of milk curd showed that preference of milk curd wasn't depending on types of milk nor its coagulant. Also, flavor preference showed better when lemon juice was added. Preference in texture of milk curd was the highest made with cow's milk and skim milk. Preference in taste was high when 10% of lemon juice was added to skim milk and preference in its appearance showed higher when the alum was added as a coagulant to both cow's milk and skim milk than other coagulants.

Pseudomonas fluorescens의 Salicylate hydroxylase를 암호하는 유전자의 클로닝

정유선,민경희,이나리 숙명여자대학교 자연과학연구소 1997 자연과학논문집 Vol.- No.8

Pseudomonas fluorescens SME11로 부터 salicylate hydroxylase를 암호하는 nahG 유전자를 클로닝하기 위하여 NAH플라스미드를 주형으로 하여 PCR을 수행하였다. PCR방법으로 얻은 1.6kb DNA절편을 pT7Blue(R)벡터에 삽입시켜 재조합 플라스미드 pNY1을 제조하였으며 이것을 E. coli에 형질전환하였다. 재조합 DNA를 제한효소로 처리하여 EcoRⅤ, KpnⅠ, PvuⅡ, StuⅠ등이 존재함을 확인하였으며, 이것으로 간단한 제한효소지도를 작성하였고 재조합 DNA를 한 방향으로 deletion하여, nahG유전자를 포함한 DNA절편의 염기서열을 결정하였다. 재조합 플라스미드인 pNY1에는 1,305bp크기의 open reading frame이 존재하였으며, salicylate hydroxylase에 해당하는 434개의 아미노산을 암호하였다. 아미노산서열을 비교한 결과, P.putida KF715와 84.1%, P.putida PpG7과 71.4%, P.putida S-1 과 48.7%의 homology를 보였으며, 또한 Sphingmonas sp.와 26.8%의 homology를 보였다. Pseudomonas fluorescens SME11 carrying NAH plasmid was isolated from wastewater. To clone nahG gene encoding salicylate hydroxylase, 1.6kb DNA fragment of PCR product from NAH plasmid was inserted to pT7Blue(R) vertor and resulting recombinant DNA was named pNY1. The transformants, E.coli SMY1, clone containing the recombinant plasmid pNY1 were able to convert salicylate to catechol and produced dark brown color caused by accumulation and auto-oxidation of catechol. Restriction endonuclease mapping of 1.6kb size of the insert of the recombinant plasmid pNY1 was carried out with EcoRⅤ, KpnⅠ, PvuⅡ, StuⅠ. By means of unidirectional ExoⅢ deletion and dideoxynucleotide chain termination, we determined the nucleotide sequence of the DNA fragment containing nahG gene. One open reading frame of 1,305 bp corresponding to 434 amino acids was found in the insert DNA. Deduced amino acid sequences of the nahG gene showed 84.1%, 71.4%, 48.7%, and 26.8% homologies to those from P.putida KF715, P. putida PpG7, P. putida S-1, and Sphingmonas sp., respectively.

Improved production of poly-γ-glutamic acid by Bacillus subtilis D7 isolated from Doenjang, a Korean traditional fermented food, and its antioxidant activity.

Lee, Na-Ri,Lee, Sang-Mee,Cho, Kwang-Sik,Jeong, Seong-Yun,Hwang, Dae-Youn,Kim, Dong-Seob,Hong, Chang-Oh,Son, Hong-Joo Humana Press 2014 Applied biochemistry and biotechnology Vol.173 No.4

<P>The objectives of this study was to improve poly-γ-glutamic acid (γ-PGA) production by Bacillus subtilis D7 isolated from a Korean traditional fermented food and to assess its antioxidant activity for applications in the cosmetics and pharmaceutical industries. Strain D7 produced γ-PGA in the absence of L-glutamic acid, indicating L-glutamic acid-independent production. However, the addition of L-glutamic acid increased γ-PGA production. Several tricarboxylic acid cycle intermediates and amino acids could serve as the metabolic precursors for γ-PGA production, and the addition of pyruvic acid and D-glutamic acid to culture medium improved the yield of γ-PGA markedly. The maximum yield of γ-PGA obtained was 24.93??0.64?g/l in improved medium, which was about 5.4-fold higher than the yield obtained in basal medium. γ-PGA was found to have 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (46.8??1.5?%), hydroxyl radical scavenging activity (52.0??1.8?%), 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonate (ABTS) radical scavenging activity (42.1??1.8?%), nitric oxide scavenging activity (35.1??1.3?%), reducing power (0.304??0.008), and metal chelating activity (91.3??3.5?%). These results indicate that γ-PGA has a potential use in the food, cosmetics, and biomedical industries for the development of novel products with radical scavenging activity. As far as we are aware, this is the first report to describe the antioxidant activityof γ-PGA produced by bacteria.</P>

Comparison of the Efficacy and Safety of Fixed-dose Combination Versus Separate Tablet Regimens in the Treatment of Tuberculous Infection

( Na Ri Jeong ),( Ji Young Kang ),( Chin Kook Rhee ),( Seung Joon Kim ),( Seok Chan Kim ),( Sook Young Lee ),( Young Kyoon Kim ),( Jong Min Lee ),( Hwa Young Lee ) 대한결핵 및 호흡기학회 2020 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.128 No.-

Background Fixed-dose combination (FDC) formulations of drugs for the treatment of tuberculosis are recommended by World Health Organization. In South Korea, the formulations have been developed recently and increasingly used. However, there have been no studies to verify the effect of the FDC on tuberculous infection in South Korea. We conducted a study to compare the efficacy and safety in the treatment of tuberculosis between FDC and separate table (ST) regimens. Methods A retrospective observational study was conducted in Seoul St. Mary's Hospital from July 2012 to May 2015. 501 active tuberculosis patients who received anti-tuberculosis medications (either as FDC or ST) were included. FDC formulation is Tubes: isoniazid 75, rifampicin 150, pyrazinamide 400, and ethambutol 275 mg/tablet) and we used it for the intensive phase according to the body weight of the patients. Results 242 patients out of 501 participants received FDC for the treatment of tuberculosis. Both of FDC and ST groups have similar baseline characteristics. FDC group had lower daily dosage of isoniazid and rifampicin than ST group by the body weight. As for the efficacy, both groups showed similar culture conversion rate at the 2-months of the treatment. There are no significant difference of the treatment outcome between the two groups (Favorable outcome: 87.2% in FDC group vs 91.9% in ST group, p=0.085). During the follow up of 1 year, recurrent rate of tuberculosis was not different between the two groups. Adverse events related to antituberculos drugs at the 8-week intensive phase of treatment occurred in the two groups with a similar frequency, including the severe adverse event requiring hospitalizations (7.92% in FDC group vs 9.09 in ST group, P=0.64). Conclusions The study provides evidence that FDC has a comparable efficacy and safety profile with ST for the treatment of tuberculosis in South Korea.

<i>Iodobacter ciconiae</i> sp. nov., a bacterium isolated from feces of oriental stork, <i>Ciconia boyciana</i>

Lee, Jae-Yun,Kang, Woorim,Kim, Pil Soo,Lee, So-Yeon,Shin, Na-Ri,Sung, Hojun,Lee, June-Young,Yun, Ji-Hyun,Jeong, Yun-Seok,Han, Jeong Eun,Jung, Mi-Ja,Hyun, Dong-Wook,Kim, Hyun Sik,Tak, Euon Jung,Kang, M Society for General Microbiology 2019 International journal of systematic and evolutiona Vol.69 No.9

<P> A novel Gram-stain-negative, facultatively anaerobic, non-motile, non-violet-pigmented, rod-shaped bacterium, designated strain H11R3<SUP>T</SUP>, was isolated from the feces of Oriental stork, <I>Ciconia boyciana</I>, collected from Seoul Grand Park Zoo, Republic of Korea. Phylogenetic analysis of the 16S rRNA gene sequence revealed that H11R3<SUP>T</SUP> formed a monophyletic clade with <I>Iodobacter fluviatilis</I> DSM 3764<SUP>T</SUP>, <I>Iodobacter arcticus</I> DSM 100243<SUP>T</SUP>, and <I>Iodobacter limnosediminis</I> DSM 103822<SUP>T</SUP>, with sequence similarities of 98.8, 98.6 and 98.4%, respectively. H11R3<SUP>T</SUP> grew optimally at 15 ℃, pH 8, with 0.5% (w/v) NaCl. The predominant isoprenoid quinone was ubiquinone-8 (Q-8), and polar lipids included phosphatidylethanolamine, three unidentified lipids, four unidentified phospholipids, and two unidentified aminophospholipids. The major fatty acids were summed feature 3 and C<SUB>16 : 0</SUB>, and the DNA G+Ccontent of the genome is 48.0mol%. The average nucleotide identity (ANI) value between strains H11R3<SUP>T</SUP> and <I>I. fluviatilis</I> NCTC 11159<SUP>T</SUP> (=DSM 3764T) is 83.7%. On the basis of phenotypic, genotypic, phylogenetic and chemotaxonomic characteristics, strain H11R3<SUP>T</SUP> represents a novel species of the genus <I>Iodobacter</I> for which the name <I>Iodobacter ciconiae</I> sp. nov. is proposed. The type strain is H11R3<SUP>T</SUP> (=KCTC 62666<SUP>T</SUP>=JCM 33283<SUP>T</SUP>). </P>

Characterization of biphenyl biodegradation, and regulation of biphenyl catabolism in Alcaligenes xylosoxydans

Lee, Na-Ri,On, Hwa-Young,Min, Kyung-Hee 숙명여자대학교 환경과학연구센터 1996 환경과학논문집 Vol.4 No.-

A strain of Alcaligenes xylosoxydans SMN3 capable of utilizing biphenyl was isolated from the sewage. This strain grew not only on biphenyl, phenol, and benzoate, but also on salicylate. The ca-tabolism of biphenyl and salicylate is interrelated, since benzoate is common metabolic in­termediates of biphenyl and salicylate. Enzyme levels in the extracts of benzoate­grown stain SMN3 cells clearly showed that benzoate induced catechol 2,3­oxygenase which in meta­cleavage enzyme of catechol, not induced catechol 1,2­oxygenase. All the oxidative enzymes of biphenly and 2,3­dihydroxybiphenyl (23DHBP) degradative pathways were induced when the cells were grown on bi­phenly or saliclyate, respectively. Biphenyl can be common inducer in the oxidation of biphenyl and 2,3­dihydroxybiphenyl, in addition salicylate was also good inducer for the 2,3­dihydroxybiphenyl dioxygenase. The two metabolic pathways for the degradation of biphenyl and salicylate are induced after growth on either biphenyl of salicylate, suggesting a common regulatory element. However, benzoate cannot induce the enzymes responsible for the oxidation of these compounds. It was clear­ly shown that biphenyl and salicylate were good inducers for indigo formation due to the activity of biphenyl dioxygenase. The strains of several bacteria that oxidize hydrocarbons including biphenyl to cis­dihydrodiols also oxidize indole to indigo. Our results suggest that indigo formation is due to the combined activities of tryptophanase and biphenyl dioxygenase.

Enantioselective Fluorination of β-Keto Phosphonates and β-Ketoesters Catalyzed by Chiral Palladium Complexes

Lee, Na-Ri,Kim, Sun-Mi,Kim, Dae-Young Korean Chemical Society 2009 Bulletin of the Korean Chemical Society Vol.30 No.4

The catalytic enantioselective electrophilic fluorinations of active methine compounds promoted chiral palladium complexes have been developed. Treatment of $\beta$-keto phosphonates and $\beta$-ketoesters with N-fluorobenzenesulfonimide as the fluorine source under mild reaction conditions afforded the corresponding $\alpha$-fluorinated adducts in high yields with excellent enantiomeric excesses (up to 99% ee). These reactions can be conducted in alcoholic solvents without any precaution to exclude water and moisture.

Characterization of biphenyl biodegradation, and regulation of iphenyl catabolism in alcaligenes xylosoxydans

Lee, Na-Ri,On, Hwa-Young,Jeong, Min-Seong,Kim, Chi-Kyung,Park, Yong-Keun,Ka, Jong-Ok,Min, Kyung-Hee The Microbiological Society of Korea 1997 The journal of microbiology Vol.35 No.2

Alcaligenes xylosoxydans strain SMN3 capable of utilizing biphenyl grew not only on phenol, and benzoate, but also on salicylate. Catabolisms of biphenyl and salicylate appear to be interrelated since benzoate is a common metabolic intermediate of these compounds. Enzyme levels in the excatechol 2. 3-dioxygenas which is meta-cleavage enzyme of catechol, but did not induce catechol 1, 2-dioxygenase. All the oxidative enzymes of biphenyl and 2, 3,-dihydroxybiphenyl (23DHBP) were induced when the cells were grown on biphenyl and salicylate, respectively. Biphenyl and salicylate could be a good inducer in the oxidation of biphenyl and 2, 3-dihydroxybiphenyl. The two enzymes for the degradation of biphenyl and salicylate were induced after growth on either biphenyl or salicylate, suggesting the presence of a common regulatory element. However, benzoate could not induce the enzymes responsible for the oxidation of these compounds. Biphenyl and salicylate were good inducers for indigo formation due to the activity of biphenyl dioxygenase. These results suggested that indole oxidation is a property of bacterial dioxygenase that form cis-dihydrodiols from aromatic hydrocarbon including biphenyl.

The Study on Miniaturization and Weight Reduction of Auxiliary Power Unit in Magnetic Levitation Train

Lee, Na Ri,Shin, Hee Keun,Choi, Sung Ho,Kim, Ju Bum,Lim, Jae Won,Park, Doh Young,Mok, Hyung Soo The Korean Society for Railway 2015 International Journal of Railway Vol.8 No.1

Due to the characteristics of the vehicle structure, the magnetic levitation train has a confined bottom space thus a study on miniaturization and weight reduction of auxiliary power unit is essential. This auxiliary power unit is an essential device used for illumination, air conditioning, heating and air brake equipment excluding the motor. The previous auxiliary power unit for magnetic levitation train has used the hard switching having a high switching frequency with heavy loss in order to reduce the size of filter reactor and transformer but the reduction in volume was not significant. In this paper, by reducing the loss, reducing the size of the cooling unit and by increasing the switching frequency using the soft switching of resonant converter, it has miniaturized and reduced the weight of filter reactor and transformer which occupy significant space in the auxiliary power unit. This study has verified the performance of 50KVA grade prototype through simulated interpretation and analysis, and compared the size and weight of auxiliary power unit of the previous magnetic levitation train.

Effect of hardness and morphology of potassium titanate on frictional wear characteristics of brake pads

Lee, Jung Ju,Kwon, Sung Wook,Kim, Jong Young,Lee, Na Ri,Pee, Jae Hwan,Kim, YooJin,Lee, Jung A.,Kim, Jeong Joo Inderscience Enterprises Ltd. 2018 International Journal of Nanotechnology Vol.15 No.6