Isolation and Identification of Staphylococcus sp. from Korean Fermented Fish Products

( Mi Na Um ),( Cherl Ho Lee ) 한국미생물생명공학회 1996 Journal of microbiology and biotechnology Vol.6 No.5

황금 발효물의 항산화 및 미백 효과 증진

엄지나 ( Ji Na Um ),민진우 ( Jin Woo Min ),주광식 ( Kwang Sik Joo ),강희철 ( Hee Cheol Kang ) 대한화장품학회 2017 대한화장품학회지 Vol.43 No.3

황금(Scutellariae baicalensis)은 항염 작용이 뛰어나 예로부터 사용되어온 약재로, 본 연구는 인삼에서 분리한 유산균 Leuconostoc mesenteroides (L. mesenteroides)을 이용해 황금 발효물을 제조하고 항산화와 미백 효과를 조사하였다. 황금 발효물은 황금을 70% 에탄올로 추출한 후에 L. mesenteroides를 접종하여 발효 제조하였다. 발효 전 황금과 황금 발효물에서 2가지 지표 성분 baicalin과 baicalein을 high-performance liquid chromatography (HPLC)을 이용하여 retention times (t_R)과 UV spectra를 확인함으로써 정성 및 정량 분석하였다. 세포 생존율 실험 결과, 발효 전후 황금 모두 독성이 확인되지 않았고 DPPH 라디칼 소거능 실험은 발효물의 SC₅₀ 값이 34.43 μ g/mL로 발효 전보다 우수한 효능을 나타내었으며, 세포 독성을 나타내지 않는 농도에서 흑색종 세포인 B16F10을 이용한 melanin 생성 억제 활성 실험 결과, 황금 발효물은 우수한 melanin 생성 억제 효과를 나타내었다(IC₅₀ = 68.17 μ g/mL). 이상의 결과들로부터 황금 발효물이 항산화 효능뿐만 아니라 미백 효능을 갖는 화장품 원료로서 개발 가능성이 있음이 시사되었다. Scutellariae baicalensis (S. baicalensis) has been traditionally used for anti-inflammatory effect. This study was designed to compare the antioxidant and whitening effects of S. baicalensis extract and its fermented extract by Leuconostoc mesenteroides (L. mesenteroides). Fermented extract of S. baicalenins was prepared by inoculation of L. mesenteroides after the extraction procedure with 70% ethanol. S. baicalensis extract and its fermented extract was investigated via high-performance liquid chromatography (HPLC). Simultaneous qualitative analysis of two bioactive components; baicalin and baicalein was achieved by comparing their retention times (t_R) and UV spectra with those of the standard components. Cell viability test results indicated that both S. baicalensis extract and its fermented extract were non-toxicity. In DPPH radical scavenging ability, SC₅₀ values of the fermented extract was 34.43 μg/mL as a result of more effective than S. baicalensis extract. In nontoxic concentration rage, fermented extract of S. baicalensis showed strong melanin production inhibitory effect in α-melanocyte stimulating hormone (MSH)-stimulated B16F10 cell (IC₅₀ = 68.17 μg/mL). These results suggested that fermented extracts of S. baicalensis has considerable potential as a cosmetics ingredient with an antioxidant and anti-wrinkle and whitening effects.

황련해독탕 발효물의 항산화, 항주름 및 미백 효과

엄지나 ( Ji Na Um ),민진우 ( Jin Woo Min ),주광식 ( Kwang Sik Joo ),강희철 ( Hee Cheol Kang ) 대한화장품학회 2017 대한화장품학회지 Vol.43 No.1

황련해독탕(HHT)은 기력을 회복하며 여러 만성질환을 예방하고 치료하기 위해 예로부터 사용되어온 약재로, 본 연구는 인삼에서 분리한 유산균 Leuconostoc mesenteroides (L. mesenteroides)을 이용해 황련해독탕 발효물(FHHT)을 제조하고 항산화, 항주름 및 미백 효과를 조사하였다. 황련해독탕 발효물은 황련해독탕을 70% 에탄올로 추출한 후에 L. mesenteroides를 접종하여 발효 제조하였다. 발효 전 황련해독탕과 황련해독탕 발효물에서 2가지 지표 성분 berberine과 palmatine을 high-performance liquid chromatography (HPLC)을 이용하여 retention times (tR)과 UV spectra를 확인함으로써 정성 및 정량 분석하였다. 세포 생존율 실험 결과, 발효 전후 황련해독탕 모두 독성이 확인되지 않았고 DPPH 라디칼 소거능 실험은 발효물의 SC₅₀ 값이 68.85 μ g/mL로 발효 전보다 우수한 효능을 나타내었다. Procollagen type I의 생성량 측정 실험에서도 역시 황련해독탕 발효물은 발효 전보다 더 높은 발현량을 나타내었으며, 세포 독성을 나타내지 않는 농도에서 흑색종 세포인 B16F10을 이용한 멜라닌 생성 억제 활성 실험 결과, 황련해독탕 발효물은 강한 멜라닌 생성 억제 효과를 나타내었다(IC₅₀ = 9.82 μ g/mL). 이상의 결과들로부터 황련해독탕 발효물이 항산화 효능, 항주름 효능뿐만 아니라 미백 효능을 갖는 화장품 원료로서 개발 가능성이 있음이 시사되었다. Hwangryunhaedoktang (HHT) has been traditionally used as a preventive and therapeutic medicine to treat enervation and diverse chronic diseases. This study was designed to compare the antioxidant, anti-wrinkle and whitening effects of HHT extract and its fermented extract by Leuconostoc mesenteroides (FHHT). FHHT was prepared by inoculation of L. mesenteroides after the extraction procedure with 70% ethanol. HHT and FHHT was investigated via high-performance liquid chromatography (HPLC). Simultaneous qualitative analysis of two bioacitive components, berberine and palmatine. was achieved by comparing their retention times (tR) and UV spectra with those of the standard components. Cell viability test results indicated that both HHT and FHHT were non-toxic. In DPPH radical scavenging ability, SC₅₀ values of the FHHT was 68.85 ㎍/mL, which is more effective than HHT. Moreover, FHHT showed higher expression in production of procollagen type I than HHT. In nontoxic concentration range, FHHT showed strong melanin production inhibitory effect in α-melanocyte stimulating hormone (α-MSH)-stimulated B16F10 cell (IC₅₀ = 9.82 μg/mL). These results suggested that fermented extracts of hwangryunhaedoktang had considerable potential as a cosmetics ingredient with an antioxidant and anti-wrinkle and whitening effects.

A multicenter study of entecavir <i>vs.</i> tenofovir on prognosis of treatment-naïve chronic hepatitis B in South Korea

Kim, Seung Up,Seo, Yeon Seok,Lee, Han Ah,Kim, Mi Na,Lee, Yu Rim,Lee, Hye Won,Park, Jun Yong,Kim, Do Young,Ahn, Sang Hoon,Han, Kwang-Hyub,Hwang, Seong Gyu,Rim, Kyu Sung,Um, Soon Ho,Tak, Won Young,Kweon Elsevier 2019 Journal of hepatology Vol.71 No.3

<P><B>Background & Aims</B></P> <P>It is currently unclear which antiviral agent, entecavir (ETV) or tenofovir disoproxil fumarate (TDF), is superior for improving prognosis in patients with chronic hepatitis B (CHB). Here, we assessed the ability of these 2 antivirals to prevent liver-disease progression in treatment-naïve patients with CHB.</P> <P><B>Methods</B></P> <P>From 2012 to 2014, treatment-naïve patients with CHB who received ETV or TDF as a first-line antiviral agent were recruited from 4 academic teaching hospitals. Patients with decompensated cirrhosis or hepatocellular carcinoma (HCC) at enrollment were excluded. Cumulative probabilities of HCC and death or orthotopic liver transplant (OLT) were assessed.</P> <P><B>Results</B></P> <P>In total, 2,897 patients (1,484 and 1,413 in the ETV and TDF groups, respectively) were recruited. The annual HCC incidence was not statistically different between the ETV and TDF groups (1.92 <I>vs</I>. 1.69 per 100 person-years [PY], respectively; adjusted hazard ratio [HR] 0.975 [<I>p</I> = 0.852] by multivariate analysis). Propensity score (PS)-matched and inverse probability of treatment weighting (ITPW) analyses yielded similar patterns of results (HR 1.021 [<I>p</I> = 0.884] and 0.998 [<I>p</I> = 0.988], respectively). The annual incidence of death or OLT was not statistically different between the ETV and TDF groups (0.52 <I>vs</I>. 0.53 per 100 PY, respectively; adjusted HR 1.202 [<I>p</I> = 0.451]). PS-matched and ITPW analyses yielded similar patterns of results (HR 1.248 [<I>p</I> = 0.385] and 1.239 [<I>p</I> = 0.360], respectively). These findings were consistently reproduced in patients with compensated cirrhosis (all <I>p</I> >0.05).</P> <P><B>Conclusions</B></P> <P>The overall prognosis in terms of HCC and death or OLT was not statistically different between the ETV and TDF groups. Further studies are needed to validate our results.</P> <P><B>Lay summary</B></P> <P>It is currently unclear which antiviral agent, entecavir or tenofovir disoproxil fumarate, is superior for improving prognosis in patients with chronic hepatitis B virus infection. In this analysis we found that there was no difference in terms of overall prognosis, including risk of hepatocellular carcinoma, death, or the need for a liver transplant, in patients receiving either antiviral.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The hepatocellular carcinoma risk was not statistically different between the ETV and TDF groups. </LI> <LI> The death or liver transplant risk was not statistically different between the 2 groups. </LI> <LI> These results were consistently reproduced after adjusting for confounding variables. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Glutathione S-transferase polymorphisms and traditional classification in Korean population with cerebrovascular disease

Um, Jae-Young,Ok, Yoon-Young,Joo, Jong-Cheon,Kim, Kyung-Yo,Kim, Na-Hyung,Hong, Seung-Heon,Kim, Hyung-Min Kyung Hee Oriental Medicine Research Center 2004 Oriental pharmacy and experimental medicine Vol.4 No.2

Glutathione S-transferase polymorphisms (GST) were examined in 98 cases with cerebrovascular disease (CVD) to test the hypothesis that GST polymorphisms confer a risk to an individual to develop CVD. Tobacco smoke is a major cause of both cancer and vascular disease. We therefore were stratified the subjects with CVD for smoking status, and then examined whether polymorphisms in this detoxification enzyme gene, GST, influence risk of CVD. Neither GSTM1 nor GSTT1 genotypes in the CVD group was significantly different from the control group (n=230), even in smokers. We attempted the combined analyses for GSTM1 and GSTT1 genotypes in CVD for smoking status. No significant association observed between the combined genotypes and CVD. We also classified the subjects and control group into four types according to Sasang Constitutional Medicine, Korean Traditional Oriental Medicine, and investigated the association among GST genotypes, CVD, and Sasang constitutional classification. Our observations do not confirm the effect of the GSTM1 and GSTT1 genotypes as a risk factor for CVD, even in smokers. Furthermore, we first attempted to evaluate the efficacy of Sasang Constitutional Medicine, and to find an association with CVD.

Effects of Moderate Intensity Exercise on the Cortical Thickness and Subcortical Volumes of Preclinical Alzheimer’s Disease Patients: A Pilot Study

Um Yoo Hyun,Wang Sheng-Min,Kim Nak-Young,Kang Dong Woo,Na Hae-Ran,Lee Chang Uk,Lim Hyun Kook 대한신경정신의학회 2020 PSYCHIATRY INVESTIGATION Vol.17 No.6

Objective We aimed to explore the impact of moderate intensity exercise on the cortical thickness and subcortical volumes of preclinical Alzheimer’s disease (AD) patients.Methods Sixty-three preclinical AD patients with magnetic resonance imaging (MRI) and 18-florbetaben positron emission tomography (PET) data were enrolled in the study. Information on demographic characteristics, cognitive battery scores, self-reported exercise habits were attained. Structural magnetic resonance images were analyzed and processed using Freesurfer v6.0.Results Compared to Exercise group, Non-Exercise group demonstrated reduced cortical thickness in left parstriangularis, rostral middle frontal, entorhinal, superior frontal, lingual, superior parietal, lateral occipital, inferior parietal gyrus, temporal pole, precuneus, insula, fusiform gyrus, right precuneus, superiorparietal, lateral orbitofrontal, rostral middle frontal, medial orbitofrontal, superior frontal, lingual, middle temporal gyrus, insula, supramarginal, parahippocampal, paracentral gyrus. Volumes of right thalamus, caudate, putamen, pallidum, hippocampus, amygdala were also reduced in Non-Exercise group.Conclusion Moderate intensity exercise affects cortical and subcortical structures in preclinical AD patients. Thus, physical exercise has a potential to be an effective intervention to prevent future cognitive decline in those at high risk of AD.

Self-organized wrinkling patterns of a liquid crystalline polymer in surface wetting confinement

Na, Jun-Hee,Kim, Se-Um,Sohn, Youngjoo,Lee, Sin-Doo The Royal Society of Chemistry 2015 SOFT MATTER Vol.11 No.24

<P>Self-organized wrinkling patterns of a liquid crystalline polymer, dictated by the chemico-physically anisotropic nature of surface wettability, are demonstrated in confined geometries. The symmetry of the geometrical constraints of the confinement primarily governs the periodic wrinkling patterns of such a polymer in the wetting region. In a circular geometry, the number of the radial domains with multi-fold symmetries is linearly proportional to the radius of the confinement. The physical origin of the wrinkling process comes from the periodic bend-splay distortions through the relaxation of the curvature elasticity.</P>

Candidate Genes of Cerebrovascular Disease and Sudden Sensorineural Hearing Loss

Um, Jae-Young,Jang, Chul-Ho,Kim, Kyu-Yeob,Kim, Su-Jin,Kim, Na-Hyung,Moon, Phil-Dong,Choi, In-Young,Myung, Noh-Yil,Jeong, Hyun-Ja,Hong, Seung-Heon,Kim, Hyung-Min SAGE Publications 2010 Clinical and applied thrombosis/hemostasis Vol.16 No.5

질산화 작용이 있는 Aeromonas hydrophila의 동정 및 특성

엄미나,장재철,유영희,지의상 한국식품영양학회 2000 韓國食品營養學會誌 Vol.13 No.6

폐수처리 중 생물학적 처리에 활용할 수 있는 질소분해 능력을 가진 미생물을 분리하여 동정하고자 경기도내 하천 6지점에서 채취한 시료로부터 50개 균주를 선별하였다. 형태학적, 생화학적 및 배양학적 실험결과 Bergey's mannual of systematic bacteriology의 색인을 통하여 Aeromonas hydrophila로 동정하였다. Aeromonas hydrophila(AH-1). (AH-3), (AH-4), (AH-6) 균이 질산화 능력이 우수하였다. 4개 균주 모두 amoxillin, ampicillin, cephalothin과 ticarcillin에 내성을 나타내었다. 본 실험에서 분리한 .Aeromonas hydrophila의 질산화의 최적조건은 균 농도 1.0×l0exp(6) cells/ml, 배양온도 37℃로 나타났다. For the purpose of the isolation of microorganisms which have the capability of nitrification, we isolated the microorganisms in 6 samples collected from the stream of Kyonggi area. 60 strains were isolated. The selected strain were identified as a Aeromonas hydrophila based on the data obtained from the morphological, biochemical and cultural characteristics defined experiments. Among them Aeromonas hydrophila (AH-1), (AH-3), (AH-4), (AH-6) showed the highest nitrification capability. All isolates were resistant to amoxillin, ampicillin, cephalothin and ticarcillin. Optimum culture conditions of isolates were 37℃ and 1×10exp(8) cells/ml for 4 hours in the nitrate medium.

A case of hemophagocytic syndrome combined with pure red cell aplasia

Na Kyung Kim,Seong Yoon Yi,Tae Hyun Um,Yee Gyung Kwak,Hye Ran Lee 대한내과학회 2009 대한내과학회 추계학술대회 Vol.2009 No.-