Original Articles : Effect of aldosterone on the amplification of oncolytic vaccinia virus in human cancer Lines

( Hyun Ju Lee ),( Ja Sung Rho ),( Shao Ran Gui ),( Mi Kyung Kim ),( Yu Kyoung Lee ),( Yeon Sook Lee ),( Jeong Eun Kim ),( Eu Na Cho ),( Mong Cho ),( Tae Ho Hwang ) 대한간학회 2011 Clinical and Molecular Hepatology(대한간학회지) Vol.17 No.3

Background/Aims: JX-594 is an oncolytic virus derived from the Wyeth vaccinia strain that causes replication-dependent cytolysis and antitumor immunity. Starting with a cross-examination of clinical-trial samples from advanced hepatocellular carcinoma patients having high levels of aldosterone and virus amplification in JX-594 treatment, we investigated the association between virus amplification and aldosterone in human cancer cell lines. Methods: Cell proliferation was determined by a cell-counting-kit-based colorimetric assay, and vaccinia virus quantitation was performed by quantitative polymerase chain reaction (qPCR) and a viral plaque assay. Also, the intracellular pH was measured using a pH-sensitive dye. Results: Simultaneous treatment with JX-594 and aldosterone significantly increased viral replication in A2780, PC-3, and HepG2 cell lines, but not in U2OS cell lines. Furthermore, the aldosterone treatment time altered the JX-594 replication according to the cell line. The JX-594 replication peaked after 48 and 24 hours of treatment in PC-3 and HepG2 cells, respectively. qPCR showed that JX-594 entry across the plasma membrane was increased, however, the changes are not significant by the treatment. This was inhibited by treatment with spironolactone (an aldosterone-receptor inhibitor). JX-594 entry was significantly decreased by treatment with EIPA [5-(N-ethyl-N-isopropyl)amiloride; a Na+/H+-exchange inhibitor], but aldosterone significantly restored JX-594 entry even in the presence of EIPA. Intracellular alkalization was observed after aldosterone treatment but was acidified by EIPA treatment. Conclusions: Aldosterone stimulates JX-594 amplification via increased virus entry by affecting the H+ gradient. (Korean J Hepatol 2011;17:213-219)

Electromagnetic Radiation Knowledge, Risk Awareness, and Shielding Practices of South Korean Occupational Therapists During Videofluoroscopic Swallowing Study: A Survey study

Na-Kyoung Hwang,Ji-Su Park,Man Seok Han,Jong-Bae Choi,Young-Jin Jung 한국자기학회 2022 Journal of Magnetics Vol.27 No.4

The videofluoroscopic swallowing study (VFSS) based on electromagnetic radiation is an instrumental test for diagnosing and rehabilitating dysphagia that is performed by many occupational therapists (OTs) in South Korea. This study aimed to investigate current electromagnetic radiation knowledge, risk awareness, and shielding practices of OTs performing VFSS and to identify educational experience on radiation protection. An online survey was conducted from April 2019 to June 2022, and a total of 69 responses were used for analysis. The mean correct score of ‘Radiation Knowledge’ was 3.24 ± 1.98 (out of 10). Mean scores of ‘Risk Awareness’ and ‘Shielding Practice’ were 2.18 ± 0.53 and 3.02 ± 0.74 (out of 5), respectively. Multiple regression analyses revealed that radiation knowledge (β = 0.292, p = 0.012) and risk awareness (β = 0.495, p < 0.001) were significant factors associated with shielding practices. Ninety-five percent of respondents had no radiation-related educational experience, and 83% reported that the reason for not participating was due to a lack of educational opportunities.

뇌졸중 재활의 조기퇴원지지(Early Supported Discharge) 서비스에 대한 고찰

황나경(Hwang, Na-Kyoung) 한국노인작업치료학회 2020 한국노인작업치료학회지 Vol.2 No.2

서론 : 본 연구는 조기퇴원지지 서비스와 그 효과성에 대해 고찰하고, 국내 뇌졸중 재활의료 현실에서 서비스도입의 필요성과 서비스 내 작업치료 접근 방향에 대한 기초자료를 제시하고자 하였다. 본론 : 조기퇴원지지는 뇌졸중 재활의료의 체계가 선진화된 여러 국가에서 시행되는 제도로 재활과 사회 자원에 대한 서비스를 병원에서 지역 사회 환경으로 조기 전환하는 것에 그 목적을 두고 있다. 경증에서 중등증 뇌졸중 환자를 서비스 대상으로 하며 환자는 조기퇴원 후 가정에서 병원과 유사한 정도의 재활서비스를 제공받는다. 선행연구들을 통해 조기퇴원지지 서비스의 의학적 효과성과 개인적, 사회적 부담을 감소시키는 비용 효율적 중재임이 보고되어 왔다. 가정과 지역사회 복귀가 가능한 경증, 중등증 환자들이 지속적으로 병원 입원을 통해 재활치료를 받고 있는 국내 뇌졸중 재활의료 현실에서 조기퇴원지지서비스는 효율적 재활의 료자원 이용과 재활의료전달체계 구축을 위한 하나의 재활의료 모델이 될 수 있다. 결론 : 향후 국내 재활의료 현실에 적합한 뇌졸중 환자의 조기퇴원지지 서비스 시범사업과 이를 통한 효과성 검증 연구가 필요할 것이다. 또한 조기퇴원지지의 근간인 가정방문재활서비스 중 현재 제도적 미비로 확립되지 못하고 있는 국내 방문작업치료 서비스에 대한 학술, 정책적 노력을 위한 논의들이 활성화될 필요가 있음을 제언한다. Introduction : This study aimed to review early supported discharge (ESD) and its effectiveness, and present the necessity of implementing an ESD service in the reality of domestic stroke rehabilitation medicine healthcare and the direction of occupational therapy approaches in ESD. Body : The ESD is a system implemented in various countries with advanced stroke rehabilitation medicine healthcare systems, and aims at early transition of rehabilitation and social resource services from the hospital to the community environment. It targets patients with mild to moderate stroke, and these patients receive rehabilitation services similar to those at home after early discharge. Previous studies have reported that ESD is a cost-effective intervention. It has medical effectiveness and reduces personal and social burdens. For patients with mild and moderate stroke can return home and to the community continuously receive rehabilitation treatment through hospital admission, ESD can be a rehabilitation healthcare model for efficient use of resources and establishment of rehabilitation medicine healthcare delivery systems. Conclusion : Pilot projects of ESD suitable for domestic rehabilitation medicine healthcare and studies to verify its effectiveness through these projects will be needed. In addition, it is suggested that discussions on academic and policy efforts for domestic home-visit occupational therapy service, which is currently not established due to institutional deficiencies, be activated.

병원급 의료기관에서 근무하는 작업치료사의 소진 경험에 대한 현상학적 연구

황나경(Hwang, Na-Kyoung),정민예(Jung, Min-Ye) 대한신경계작업치료학회 2020 재활치료과학 Vol.9 No.4

목적 : 본 연구는 현상학적 연구방법을 통해 병원급 의료기관에서 근무하는 작업치료사의소진의 본질을탐구함으로써 소진을 완화하기 위한 방법을 개발하는데 필요한 기초자료를 제공하고자 한다. 연구방법 : 병원급 의료기관에서 근무하는 작업치료사 6명을 대상으로 8주간 심층면담을 실시하였다. 면담자료는 Colaizzi의 현상학적 연구방법을 통해 분석하였고, 자료 전사 및 주제생성과, 범주화 과정에파랑새 2.0 소프트웨어를 사용하였다. 결과 : 수집된 자료를 통해 14개의 주제, 9개의 주제모음, 3개의 범주를 도출하였다. 분석 결과, 소진의원인은 병원 작업치료 업무에서 오는 소진, 업무 환경에서 오는 소진 등으로 확인되었고, 소진이 개인의삶에 미치는 영향은 내면 심리변화와 타인에 대한 영향이었다. 소진에 대한 대처 전략으로는 개인 삶의풍요화, 자기 인식과 철학, 인식적 접근, 사회적 지지, 삶의 새로운 목표 설정 등으로 나타났다. 결론 : 반복되는 재활과정에서 오는 타성과 치료 효과에 대한 확신 부재에서 경험하는 직업 정체성 갈등을해결하고 작업치료의 가치 제고를 위한 자생적 노력이 필요함을 제언한다. 또한 소진예방을 위한조직 차원의 규정 및 교육 프로그램 마련과 수평적인 인간관계를 지향하는 조직문화의 확립이필요할 것이다. Objective : This study aimed to understand the burnout experience of hospital occupational therapists through phenomenological research. Methods : We conducted in-depth interviews with 6 occupational therapists working at hospital for 8 weeks. The data were analyzed using the Palangsae 2.0 software to derive meaning through the process of transcription and data entry, coding, and topic creation. Results : From the collected data, 14 sub-themes, 9 theme clusters, 3 categories were identified. The cause of burnout was confirmed as being due to therapy-related work and workplace conditions. The impacts of burnout on individual life were psychological in nature and also included being an adverse, influence on others. The coping strategies to deal with burnout were enrichment of personal life, self-awareness and philosophy, conscious approach, social support, and setting new goals for life. Conclusion : We suggest that spontaneous efforts are needed by occupational therapists in order to alleviate the conflicts of job identity. In addition, organizational regulations and programs and the establishment of horizontal organizational cultures are required to overcome and prevent burnout of occupational therapists.

뇌졸중 및 노인 환자의 퇴원 평가도구에 대한 체계적 고찰

황나경(Hwang, Na-Kyoung),유은영(Yoo, Eun-Young) 대한신경계작업치료학회 2020 재활치료과학 Vol.9 No.3

Objective : The aim of this systematic review is to analyze the assessment items and tools used for the discharge of stroke and elderly patients and to help select appropriate assessment tools for discharge planning. Methods : The studies included in this review were collected from the PubMed, Medline Complete (EBSCOhost), and Scopus databases from January 2009 to December 2018. A total 22 studies were selected. The research types, the areas and sub-areas of assessment, as well as the assessment tools according to the areas were analyzed. Results : Descriptive research (59.2%) was the most frequent type of research. The 12 main areas of assessment were medical condition, activities of daily living, swallowing function, sensation, mobility, cognition and perception, communication, emotion, home environment, patient knowledge and readiness for discharge, social support, and well-being. Among the assessment tools, the most frequently used tools were Bathel Index; Functional Independence Measure, to assess activities of daily living; Short-Form Health Survey, to assess well-being, and Timed Up and Go test, to assess mobility. Conclusion : This study will help select the assessment areas and tools to be considered at discharge of stroke patients and serve a basis for the development of comprehensive assessment tools for discharge planning. 목적 : 본 연구는 뇌졸중 환자와 노인 환자를 대상으로 퇴원 시기에 이루어지는 평가도구에 대한 체계적문헌고찰을 통해 평가 항목과 평가도구들을 분석하여 효율적인 퇴원계획을 위한 기초자료를 제공하고자한다. 연구방법 : 문헌 선정은 PubMed, Medline Complete(EBSCOhost), Scopus를 사용하여 2009년 1월부터2018년 12월까지의 온라인 데이터에 등록된 연구를 대상으로 최종 22편의 연구를 선정하였고, 연구설계 및 퇴원 시 주요 평가영역, 하위영역, 평가도구들을 분석하였다. 결과 : 선택된 연구들의 연구 설계 형태는 기술적 연구가 13편(59.2%)으로 가장 많았다. 퇴원 시 평가영역은 의학적 상태, 일상생활활동, 삼킴 기능, 감각, 이동성, 인지·지각, 의사소통, 감정상태, 가정환경, 환자의 지식과 퇴원을 위한 준비, 사회적지지, 안녕감 등 12개 영역으로 나타났다. 평가 영역에 따라 사용된 평가도구를 분석한 결과, 가장 높은 빈도의 사용을 보이는 평가도구는 일상생활활동을 평가하는 Barthel Index, Functional Independence Measure, 안녕감을 평가하는 Short Form Health Survey, 이동성을 평가하는 Timed Up and Go test 등으로 나타났다. 결론 : 본 연구를 통해 뇌졸중, 노인 환자의 퇴원 시 이루어지는 다양한 평가 영역과 평가도구의 사용과 제한적이나 여러 영역을 포괄하는 평가도구들을 확인할 수 있었다. 이는 뇌졸중 환자의 퇴원 시 고려되는 평가 영역과 효과적인 평가도구의 선택을 돕고 적절한 퇴원계획 중재와 제안을 위한 포괄적인 평가도구의 개발의 기초자료가 될 것이다.

국내 작업치료사의 퇴원계획 개입에 대한 실태 조사연구

황나경(Hwang, Na-Kyoung),유은영(Yoo, Eun-Young) 대한신경계작업치료학회 2020 재활치료과학 Vol.9 No.2

목적 : 본 연구는 환자의 퇴원계획에 대한 작업치료사 개입의 필요성과 퇴원 스크리닝과 계획을 위해고려되어야할 영역을 조사함으로써 효과적인 퇴원계획 실행을 위한 퇴원평가도구의 개발의 기초 자료를제공하고자 실시되었다. 연구방법 : 연구 참여에 동의한 의료기관에서 근무하는 작업치료사 60명을 대상으로 온라인 설문 조사를실시하였다. 설문 내용은 일반적 특성, 퇴원계획 현황, 퇴원평가와 퇴원계획 필요성, 총 36문항으로구성되었다. 수집된 자료는 SPSS 20.0 프로그램을 이용하여 기술통계, 독립표본 티 검증, 일원배치분산분석, 사후검증은 쉐페 검증으로 분석하였다. 결과 : 퇴원계획과 준비에 대한 작업치료사의 역할과 퇴원평가도구의 필요성에 대한 인식은 높게 나타났으나, 작업치료사의 퇴원 관련 지식 및 정보 보유에 대한 인식 정도는 낮은 것으로 조사되었다. 퇴원계획중재 시 겪게 되는 어려움은 면담과 평가를 위한 적절한 수가의 부재, 퇴원계획을 위한 팀 접근의 부족, 퇴원계획을 위한 적절한 평가도구의 부재에 대한 응답이 높게 나타났다. 퇴원 시 평가의 필요성이 높은영역은 낙상위험과 BADL수행으로 나타났으며, 평가의 필요성이 낮은 영역은 안녕감과 발병 전 기능수준으로 나타났다. 결론 : 본 연구를 통해 퇴원계획에 대한 작업치료사 개입의 필요성을 파악할 수 있었으며, 향후 효과적인퇴원계획 실행을 위한 퇴원평가도구 개발의 기초자료가 될 것으로 사료된다. Objective : The purpose of this study was to investigate the necessity of occupational therapist’s involvement in patient discharge planning, the areas that should be considered for discharge screening and planning, and to provide the basic data required for the development of a discharge assessment tool. Methods : We conducted an online questionnaire survey of 60 occupational therapists who were working at medical institutions and had agreed to participate in the study. The questionnaire was composed of 36 questions regarding the general characteristics of the current discharge planning process and the necessity of discharge assessment and planning. Descriptive statistics, an independent t-test, and a one-way ANOVA were conducted using SPSS 20.0. As for the post-hoc test, Scheffe’s test was used. Results : The awareness of occupational therapist s role in discharge planning and the necessity of a discharge assessment tool were high, but the occupational therapist s awareness of discharge-related knowledge was low. The difficulties in discharge planning showed high response rate in the absence of adequate fee-for-service in the patient interview and assessment and the lack of team approach and appropriate assessment tools for discharge planning. The high-needs areas for evaluation during discharge were fall risk and BADL, and the low-needs areas were well-being and functional level prior to onset. Conclusion : This study is expected to provide preliminary information necessary for the development of a discharge assessment tool for effective discharge planning.

Can high serum anti-M${\ddot{u}}$llerian hormone levels predict the phenotypes of polycystic ovary syndrome (PCOS) and metabolic disturbances in PCOS patients?

Hwang, Yu Im,Sung, Na Young,Koo, Hwa Seon,Cha, Sun Hwa,Park, Chan Woo,Kim, Jin Yeong,Yang, Kwang Moon,Song, In Ok,Koong, Mi Kyoung,Kang, Inn Soo,Kim, Hye Ok The Korean Society for Reproductive Medicine 2013 Clinical and Experimental Reproductive Medicine Vol.40 No.3

Objective: To evaluate correlations between serum anti-M${\ddot{u}}$llerian hormone (AMH) levels, phenotypes of polycystic ovary syndrome (PCOS), obesity, and metabolic parameters in patients with PCOS. Methods: A total of 175 patients with PCOS were diagnosed according to the Rotterdam Consensus were included. Exclusion criteria were age over 40, FSH>25 mIU/mL, and 17a-OHP>1.5 ng/mL. The Phenotypes of PCOS were divided into a severe form (oligo-anovulation, ANOV/hyperandrogenism/polycystic ovary morphology [PCOM]; n=59) and a mild form without HA (ANOV/PCOM, n=105). The serum AMH levels were classified into 3 groups (<5 vs. 5-10 vs. >10 ng/mL). Obesity was defined as body mass index (BMI) ${\geq}25kg/m^2$ (n=34). Results: The mean age was $25.9{\pm}5.7$ year and mean AMH level was $10.1{\pm}5.4$ ng/mL. The BMI ($kg/m^2$) was higher in group 1 ($24.2{\pm}6.3$) than in group 2 ($21.9{\pm}4.3$, p=0.046) or group 3 ($21.6{\pm}3.3$, p=0.019). There was no difference among the three groups in age, menstrual interval, antral follicle counts, androgens, or other metabolic parameters. The obesity group showed significantly lower AMH ($7.7{\pm}3.9$ ng/mL vs. $10.7{\pm}5.6$ ng/mL), p=0.004) and low-density lipoprotein levels ($93.1{\pm}21.2$ mg/dL vs. $107.5{\pm}39.3$ mg/dL, p=0.031), and showed higher total T ($0.74{\pm}0.59$ L vs. $0.47{\pm}0.36$ ng/mL, p=0.001), free T ($2.01{\pm}1.9$ vs. $1.04{\pm}0.8$ pg/mL, p=0.0001), and free androgen index ($6.2{\pm}7.9$ vs. $3.5{\pm}3.0$, p=0.003). After controlling for age factors and BMI, the serum AMH levles did not show any significant correlations with other hormonal or metabolic parmeters. Conclusion: For PCOS patients under the age 40, serum AMH is not negatively correlated with age. High serum AMH levels can not predict the phenotype of PCOS and metabolic disturbances in PCOS patients in the non-obese group. Further study might be needed to define the relation more clearly.

Peptide-loaded nanoparticles and radionuclide imaging for individualized treatment of myocardial ischemia.

Hwang, Hyosook,Kwon, Jeongll,Oh, Phil-Sun,Lee, Tai-Kyoung,Na, Kyung-Suk,Lee, Chang-Moon,Jeong, Hwan-Seok,Lim, Seok Tae,Sohn, Myung-Hee,Jeong, Hwan-Jeong Radiological Society of North America 2014 Radiology Vol.273 No.1

<P>To determine whether chitosan hydrogel nanoparticles loaded with vascular endothelial growth factor (VEGF) peptides (81-91 fragments) capable of targeting the ischemic myocardium enhance angiogenesis and promote therapeutic effects and whether radionuclide image-guided dosage control is feasible.</P>

A Novel Integrative Expression Vector for Sulfolobus Species

( Kyoung Hwa Choi ),( Sung Min Hwang ),( Na Eun Yoon ),( Jae Ho Cha ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.11

With the purpose of facilitating the process of stable strain generation, a shuttle vector for integration of genes via a double recombination event into two ectopic sites on the Sulfolobus acidocaldarius chromosome was constructed. The novel chromosomal integration and expression vector pINEX contains a pyrE gene from S. solfataricus P2 (pyrEsso) as an auxotrophic selection marker, a multiple cloning site with histidine tag, the internal sequences of malE and malG for homologous recombination, and the entire region of pGEM-T vector, except for the multiple cloning region, for propagation in E. coli. For stable expression of the target gene, an α-glucosidase-producing strain of S. acidocaldarius was generated employing this vector. The malA gene (saci_1160) encoding an α-glucosidase from S. acidocaldarius fused with the glutamate dehydrogenase (gdhAsaci) promoter and leader sequence was ligated to pINEX to generate pINEX_malA. Using the “pop-in” and “pop-out” method, the malA gene was inserted into the genome of MR31 and correct insertion was verified by colony PCR and sequencing. This strain was grown in YT medium without uracil and purified by His-tag affinity chromatography. The α-glucosidase activity was confirmed by the hydrolysis of pNPαG. The pINEX vector should be applicable in delineating gene functions in this organism.

Case Reports : Visualization of Jejunal Bleeding by Capsule Endoscopy in a Case of Eosinophilic Enteritis

Na Young Kim,Jin Wook Kim,Jin Hyeok Hwang,Dong Ho Lee,Hye Seung Lee,Kyoung Ho Lee,Sung Won Kim 대한내과학회 2005 The Korean Journal of Internal Medicine Vol.20 No.1