"하고초(夏枯草)"의 생약학적 연구

황명석,조창희,박종희,Whang, Myung-Suk,Cho, Chang-Hee,Park, Jong-Hee 한국생약학회 2000 생약학회지 Vol.31 No.2

'Ha Go Cho (夏枯草)' is one of the Chinese crude drugs used mainly as a diuretic. With regard to the botanical origin of 'Ha Go Cho', it was reported by Su et al. that those from China were originated from the fruited spica of Prunella vulgaris L. of Labiatae. It was, however, for the herba or spica of Prunella vulgaris L. var. lilacina Nakai from Korea. According to survey of markets in Korea, most of the materials collected in the markets seemed to be originated from Prunella plant, while some seemed to be Thesium plant of Santalaceae. To clarify the botanical origin of 'Ha Go Cho', the anatomical characteristics of Prunella vulgaris L. var. lilacina Nakai and Thesium chinense Turcz. were studied. As a result, it was clarified that some 'Ha Go Cho' from Korea were the herba or spica of Prunella vulgaris var. lilacina, whereas some others were the herba of Thesium chinense.

아말감 충전후 두발 및 소변내 수은 함량에 관한 연구

조명숙,송근배,윤희숙 대구보건대학 1992 대구보건대학 論文集 Vol.13 No.-

The purpose of the present study was to determine the mercury in human scalp hair and urine from amalgam fillings, sixty-five subjects were examined their oral status, alginate impression were taken to make dental arch stone models and calculated their surface area of amalgam fillings. The 28 control subjects had no amalgam filling and 37 compare subjects had an average of 12 surfaces of amalgam. The morning urine and scalp hair were collected from all subjects and samples were digested in acid digestion bomb(Parr Instrument Company, U.S.A.) The mercury contents were determined with atomic absorption spectrophotometer(Model 551, IL Co., U.S.A.) and atomic vapor accessory (Model 440, IL Co., U.S.A.) . The results obtained were as follows ; 1. The urinary mercury contents of control group(no amalgam in oral cavity) were 2±10.0㎍/g. creatinine/L and compare group(amalgam filling) were 16.7±16.0㎍/g. creatinine/L(p<0.05). 2. The scalp hair mercury contents of amalgam filling group(3.29±1.03㎎/L) were slightly higher than those of the amalgam free group(3.04±1.11㎎/L) but not significant difference(p>0.05). 3. The linear correlation coefficient was 0.3490 and significantly different between mercury content of scalp hair and amalgam surface area(p<0.05), but had no significant difference between urinary mercury contents and amalgam surface area(r=0.0934, p>0.05).

의상디자인 교육용 하이퍼미디어 코스웨어 개발연구

조진숙,한명숙 복식문화학회 2000 服飾文化硏究 Vol.8 No.5

This paper focuses on the development of a fashion design educational program that will faster the talent and aesthetic consciousness of fashion design students. The goal of progressive educational methods with cutting edge technology is to elevate educational efficiency, and ultimately rear creative designers. To fulfill its objective, this paper presents a courseware program on fashion design education utilizing hyper media that include animation, photographs, pictures, sounds and characters. By the utilization of such comprehensive source of information media, a hypermedia courseware was developed fur elementary education in fashion design with the following conclusions: 1.The program enables the students to customize their fashion design education, The students of fashion design education programs are able to choose and adjust their own programs to suit their requirements and intellectual level. 2.The program effectively motivates. Much like a constant sequence of various images on a screen that can provoke profound heart-felt emotions and inspire creativity, the program can stimulate and motivate the students. 3.The program provides a consistent education. To advance the overall quality of fashion design education, the problem of dissonance in educational content should be resolved and possible individual errors in delivery rectified. The present program will solve such problems, and establish coherent standards that will withstand the test of time regardless of time or place. 4.The program is cost effective. Since the present program frees the teacher from the time consuming drudgery of preparing various texts and lesson plans, have the materials delivered and handled, it reduces the time and cost previously required. 5.The program has the capability to be upgraded. The accumulated know-how of teachers and the total experience of the program can be linked with other programs for limitless expansion.

경견완 장애를 보이는 영상 단말기근로자들의 심리적 특성에 따른 분류와 업무지각특성

조선미,이세휘,임기영,이명숙 용인정신의학연구소 1999 용인정신의학보 Vol.6 No.2

양파(Allium cepa L.) 추출물이 Tyrosinase 유전자 발현에 미치는 효과

조남철,윤연희,이혜진,손현정,김양경,최근희,나명석,조영권,이황희,진종언 한국식품영양학회 2001 韓國食品營養學會誌 Vol.14 No.3

Melanin 생성에 관여하는 tyrosinase 유전자의 발현을 억제하는 물질을 탐색하고자 tyrosinase promoter를 지닌 Bl6 mouse melanoma cell해 앙파 methanol 추출물을 처리한 바 양파 methanol 추풀물은 10.0㎍/ml, 100.0 ㎍/ml, 1.0 ㎎/ml의 농도에서 대조군에 비해서 약 15%, 23% 57%의 억제효과를 나타냈으며 세포생존율은 1.0 ㎍/ml, 10.0 ㎍/ml, I00.0 ㎍/ml, 1.0㎎/ml의 농도에서 약 126 %, 92%. 85%, 64%로서 세포독성이 낮게 나타났다. Ethyl acetate, butyl alcohol, 그리고 물 용매 분획물은 tyrosinase 유전자의 발현물 억제하는 효과가 없었지만 methylene chloride 용해 분획물은 10.0 ㎍/ml과 100.0 ㎍/ml의 농도에서 약 37%와 35%의 발현율을 나타냄으로서 대조군에 비해 크게 억제하였다. Onion(Allium cepa L,) extract by methanol repressed the expression of tyrosinase gene of B16 mouse melanoma cell containing tyrosinase promoter. 10.0 ㎍/ml, 100.0 ㎍/ml, 1.0 ㎎/ml of the extract repressed expression of tyrosinase gene about 15%, 23%, and 57%, respectively, compared with control. In the MTT assay, the same extract exhibited low cytotoxicity at 1.0 ㎍/ml, 10.0 ㎍/ml, 100.0 ㎍/ml, and 1.0 ㎎/ml, respectively. The fractions of ethyl acetate, butyl alcohol, and water did not showed the repressive effect on the expression of tyrosinase gene, but the fraction of methylene chloride repressed highly at 10.0 ㎍/ml and 100.0 ㎍/ml.

인접한 고층건물의 豊荷重 상호간섭에 관한 연구

조강표,정진택,정명채,김윤석 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

Wind load is evaluated based on Standard Design Loads for Building of AIK(Architectural Institute of Korea), which was obtained from wind-tunnel experiment for an isolated building in open country, actual wind load can be different from the code because of terrain, building geometry, wind direction, separation distance between the buildings. Among them. separation distance is the most important factor. This paper presents the interference factor for sqaue section high-rise building structure, focusing on the separation distance. Interference factor was obtain from the force balance test in a wind tunnel.

이동 열원을 고려한 전자빔 용접의 유한요소해석

조해용,정석영,김명한,조창용,이제훈,서정 한국레이저가공학회 2001 한국레이저가공학회지 Vol.4 No.1

Simulation on the electron beam welding of Al 2219 alloy was carried out by using commercial FEM code MARC, which encounters moving heat sources. Due to axisymmetry of geometry, a half of the cylinder was simulated. A coupled thermo-mechanical analysis was carried out and subroutine for heat flux was substituted in the program. The material properties such as specific heat, heat transfer coefficient and thermal expansion coefficient were given as a function of temperature and the latent heat associated with a given temperature range is considered. As a result, the proper beam power is 60kVX60mA and welding speed is 1~1.5 m/min. The residual stress in the heat-affected zone as well as the fusion zone does not increase. It is necessary to use jigs for preventing distortion of cylinder and improving weld quality.

지단백과 알부민 및 이들의 변형물질이 메산지움세포 증식에 미치는 영향

손일석,이태원,박재경,김희진,조병수,임천규,김명재 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.2

목 적 : 혈청 지단백과 알부민은 그 자체로써 또는 산화나 당화 과정에 의한 변형을 거쳐 사구체 질환을 진행시킬 수 있는 것으로 알려져 있다. 그 기전은 분명하지는 않으나 메산지움세포 증식과 관련이 있는 것으로 알려져 있다. 저자들은 비교적 간단한 흡광도측정법을 이용하여 지단백과 알부민 및 그들의 변형물질들이 각각 메산지움세포 증식에 미치는 영향을 알아보고자 하였다. 방 법: 정상인의 혈청 지단백을 원심분리 하여 저밀도지단백, 고밀도지단백, 초저밀도지단백을얻고, 산화 저밀도지단백, 당화 저밀도지단백, 그리고 혈청 알부민과 당화 알부민을 각각 농도를 달리하여 배양 중인 인간 메산지움세포에 가한 후 흡광도를 측정함으로써 메산지움세포의 증식정도를 알아보았다. 양성 대조군으로 interleukin-1β를 이용하였다. 결 과: 저밀도지단백은 농도 증가시 50 μg/mL와 100 μg/mL에서 세포의 증식을 보였으며,고밀도지단백과 초저밀도지단백은 농도에 따른 유의한 변화가 없었다. 산화 저밀도지단백은5 μg/mL에서부터 세포의 증식을 초래한 후 10 μg/mL와 25 μg/mL에서도 이를 유지하였다. 당화 저밀도지단백은 농도 증가에 따라 10 μg/mL와 100 μg/mL에서 세포의 증식을 억제하였다. 알부민은 농도 500 μg/mL에서 세포 증식을 억제하다가 고농도(1,000 μg/mL)에서는 영향이 없었으며, 당화 알부민 역시 100 μg/mL의 적은 농도에서는 억제효과를 보였으나 고농도(1,000 μg/mL)에서는 오히려 증식을 일으키는 결과를 보였다. 결 론: 저농도에서 저밀도지단백과 산화 저밀도지단백은 사람 메산지움세포의 증식을 초래하였으며, 당화 저밀도지단백은 증식을 억제하였다. 알부민은 일정농도까지 사람 메산지움세포의 증식을 억제하다가 고농도에서는 영향이 없었으며, 당화 알부민도 일정농도까지 증식 억제 효과가 있다가 고농도에서는 오히려 증식을 초래하는 양상을 보였다. Background : Modified lipoproteins may be involved in nephro- and glomerulosclerosis. Diabetic nephropathy-like lesions have also been induced in a rat model by glycated and glycoxidized albumin. In cultured rat or human mesangial cells, enhanced cell proliferation and production of mesangial matrix in response to lipoproteins and their modified forms have been demonstrated by [3H]-thymidine incorporation and cell counting assays. But these methods are relatively complex and most of them have used only one or two of the lipoprotein, albumin and their modified forms. Methods : We investigated the effects of native and modifed lipoproteins, and albumin on cultured human mesangial cell proliferation using non-radioactive colorimetric method by MTS/PMS assay. Lipoproteins added were low density lipoprotein(LDL), high density lipoprotein(HDL), very low density lipoprotein(VLDL), oxidized LDL(oxidation with copper sulfate in vitro) and glycated LDL and we also used albumin, glycated albumin, and interleukin-1β as a positive control. Results : Interleukin-1β promoted the proliferation of cultured human mesangial cells up to concentration 20 ng/mL. LDL induced the proliferation of mesangial cells in a concentration-dependent manner up to concentration 100 μg/mL. HDL and VLDL had no significant proliferative effect. Oxidized LDL caused the proliferation of mesangial cells at low concentration up to concentration 25 μg/mL. Addition of glycated LDL resulted in a concentrationdependent inhibition of mesangial cells. Albumin and glycated albumin inhibited the proliferation of mesangial cells at low concentration of 100 μg/mL, but cell growth was increased at higher concentrations. Conclusion : We demonstrated the effects of the single and modified proteins on the proliferation of cultured human mesangial cell by relatively simple colorimetric method. Results were almostly identical to those of previous studies obtained by radioactive method or cell counting assay. (Korean J Nephrol 2002;21(2):266-275)

산마늘로부터 단리한 kaempferol과 quercetin의 콜레스테롤 저하 활성

이성숙,문서현,이학주,최돈하,조명행 한국목재공학회 2004 목재공학 Vol.32 No.1

식용 임산자원인 산채류를 기능성 식품으로 개발하고자 산마늘을 비롯한 충 13종의 에탄을 조추출물에 대한 콜레스테롤 저하활성을 검정하였다. 즉, 콜레스테롤 생합성 과정 초기에 관여하는 3-hydroxy-3-methylglutaryl coenzyme A reductase(HMG-CoA reductase)와 후기에 관여하는 squalene synthase의 효소 활성을 조사한 결과 산마늘 잎 에탄올 추출물이 두 효소의 활성을 공히 70% 이상 저해하여 활성이 가장 우수한 것으로 나타났다. 그리고 이러한 콜레스테롤 저하활성과 관련이 있는 물질을 탐색하고자 산마늘로부터 물질 단리를 시도하여 디클로로메탄 가용부로부터 kaempferol과 quercetin을 단리하였다. 또한 유전자 레벨에서의 콜레스테롤 저하 활성을 조사하기 위해 단리물질과 분획물을 C100세포(햄스터 유래 HMG-CoA reductase 고발현 세포주)에 각각 5㎍/㎖과 10㎍/㎖로 24시간 처리하여 HMG-CoA reductase와 squalene synthase의 mRNA 발현 정도를 조사하였다. 그 결과 10㎍/㎖로 kaempferol과 quercetin을 처리한 경우 두 효소의 mRNA가 전혀 발현하지 않는 것으로 나타나 유전자 레벨에서의 콜레스테롤 생합성 저해 효과를 확인할 수 있었다. 이상의 결과 산마늘 잎 에탄올 추출물은 콜레스테롤 생합성에 관여하는 HMG-CoA reductase와 squalene synthase의 활성을 저해하며 이러한 활성 저해 효과는 kaempferol과 quercetin에 기인하는 것으로 사료되었다. 특히 kaempferol과 auercetin은 여러식물의 성분으로서 이미 알려진 화합물이지만 콜레스테롤 저하활성이 있는 것으로 밝혀진 것은 이번이 처음으로 금후 이들 물질과 이들 물질을 함유하고 있는 식물 활용에 필요한 자료를 제공하였다고 사료된다.

천연물 복합체 추출물(알러큐)의 알러지 유발 흰쥐에 대한 항알러지 효과

서경석,권명상,조정순 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.5

Medicinal plants are of great importance in providing healthcare to a large portion of the population in Korea. A number of plants are described in Dong-Ui-Bo-Gam for use in the treatment of allergic disorders, namely psoriasis, eczema, bronchial asthma, etc. In this study, we evaluated the effect of AIIerQ, which is multi-complexes of various plants extracts such like Mori folium, Scutellaria baicallensis, Glycyrrhiza uralnsis, Mentha sacharinensis and Poncirus trifoliata on compound 48/80 induced anaphylactic shock, ovalbumin induced asthma in vivo and anti-IgE antibody induced hypersensitivity in vitro. We found antianaphylactic or antiallergic properties of AIIerQ when given orally. AIIerQ for prophylactic treatment for anaphylactic shocks have produced good results. AIIerQ may modulate various aspects of immune function and allergic inflammation. In the present study, we analyse the effects of AIIerQ on mast cell degranulation, mortality, cAMP/cGMP, O₂, H₂O₂ level, cyokine production and on the elicitation of IgE-mediated mast cell-dependent allergic inflammation in vivo and in vitro. We have established that AIIerQ inhibited histamine release, cAMP/cGMP, O₂, H₂O₂ level, IL-4, tumor necrosis factor-alpha(TNF-α) and IL-6 production without having any significant physical change. These effects have been observed in mast cell(in vitro) and serum(in vivo) derived from three different origins that were activated by either immunological or non-immunological stimuli. These results suggest that the antianaphylactic and antiasthmatic action of AIIerQ may be associated with an increase in the intracellular inhibition of the cAMP phosphodiesterase. Furthermore, AIIerQ identified as potent inhibitors on 0₂, H₂O₂ and cytokine activity. these data suggest that AIIerQ may have an inhibitory role in mast cell-mediated allergic inflammation, and thus might be considered as an useful functional food.