배양 마우스 신경세포에서 phenylpropanoid 유도체의 NMDA 및 산소/포도당 결핍 유발 손상에 대한 방어효과

위명복 제주대학교 생명과학연구소 1998 제주생명과학연구 Vol.1 No.-

The phenylpropanoids, naturally occurring compounds, have been known to contain the aromatic ring to which a three-carbon side chain is attached and various pharmacological actions. In our previous studies, we demonstrated that eugenol, one of the phenylpropanoid compounds has the neuroprotective effects in the in vitro and in vivo ischemia models. In this study, we studied whether other phenylpropanoid derivatives, myristicin, 4-allylanisole, α-asarone, and β-asarone have the similar neuroprotective action on N-methyl-D-aspartate (NMDA) or oxygen/glucose deprivation (OGD)-induced neurotoxicity in cultured mixed cortical Neurons. Between the 30μM and 300μM concentration, myristicim, 4-allyanisole, α-asarone reced LDH release by 9-95%, 0-88%, 14-77% and 15-98% against the NMDA(300 μM)-induced acute neurotoxicity, respectively. However, in the OGD paradyme, only both 4-allylanisole and α-asarone, significantly(p<0.05) showed 27% and 30% neuroprotective effect at the maximal concentration (300μM),respectively. These results suggest that phenylpropanoid derivatives may offer a helpful contribution for the improvement of the glutamate-related neurodegenerative diseases.

조직기업가정신에 관한 실증연구

윤종록,위홍복,염명곤 동의대학교 경제경영전략연구소 2004 經濟經營硏究 Vol.2 No.1

This research proposed and tested a conceptual model that examined the difference between entrepreneurship and organizational effectiveness. The result are as follows. First, Entrepreneurship has influenced significantly to organizational commitment, job satisfaction and creativity. Second, according to dynamic environment, entrepreneurship has influenced significantly to a organizational commitment and job satisfaction, but has not influenced significantly to a creativity. Lastly, according to risk-taking of CEO, entrepreneurship has influenced significantly to a job satisfaction, but has not influenced significantly to an organizational commitment and creatively. Therefore, a variables of dynamic environment and personality of CEO have showed a role as moderator in the relationship between entrepreneurship and organizational effectiveness.

Increased expression of P53 and Bax in the spinal cords of rats with experimental autoimmune encephalomylitis

문창종,김승준,위명복,김희석,정종태,박전홍,지영흔,Tanumab, Naoyuki,Matsumoto, Yoh,신태균 濟州大學校 農科大學 動物科學硏究所 2000 動物科學論叢 Vol.15 No.1

The expression of pro-apoptotic molecules, p53 and Bax, in the spinal cord of rats with experimental autoimmune encephalomyelitis (EAE) was examined. Apoptosis was confirmed by the terminal deoxynucleotidyl transferasemediated dUTP nick end-labeling (TUNEL) method. TUSEL (+) apoptotic cells were mainly either ED1 (+) macrophages or T-cells in the parenchyma of E N . Western blot analysis showed that both p53 and Bax expression significantly ( p < 0.01) increased in the spinal cords of EAE rats at the peak stage, and thereafter declined. An immunohistochemical study showed that inflammatory cells (notably T cells) in the parenchyma express p53 and Bax, while brain cells, includng neurons and glia, were devoid of these nuclear staining of these molecules. The nuclear expression of p53 largely matches apoptotic cells in the parenchyma of EAE. These finchngs suggest that pro-apoptotic molecules, p53 and Bax, may play an important role in eliminating T cells in the parenchyma in EAE.

Acute Effects of Korean Earthworm(Lamnodrilus gotai Hatai) Juice on Blood Pressure and Spontaneous Motor Activity in Spontaneously Hypertensive Rats

Wie, Myung-Bok,Song, Dong-Keun,Kim, Yung-Hi The Korean Society of Pharmacognosy 1993 생약학회지 Vol.24 No.3

We investigated the effects of single administration of Korean earthworm(Lamnodrilus gotai Hatai) juice (KEJ) on blood pressure and spontaneous motor activity in spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats. We report that KEJ lowered blood pressure and spontaneous motor activity in SHR.

Eugenol Inhibits Excitotoxins-Induced Delayed Neurotoxicity, Oxidative Injury and Convulsion

Myung-Bok Wie,Byung-Hwa Cheon,Seon-Young Lee,Kun Ho Son,Dong-Keun Song,Taekyun Shin,Hyoung-Chun Kim 한국독성학회 2006 Toxicological Research Vol.22 No.3

In previous our studies, we have reported that eugenol derived from Eugenia caryophyllata (Myrtaceace) exhibits acute N-methyl-D-aspartate (NMDA)- and oxygen/glucose deprivationinduced neurotoxicity in primary cortical cultures and protects hippocampal neurons from global ischemia. In this study, we investigated whether the extracts and fractions of E. caryophyllata or eugenol shows the neuroprotective effects against delayed neuronal injury evoked by NMDA or α-amino-3-hydroxy-5-methylisoxazole propionate (AMPA), and oxidative damage induced by arachidonic acid-, hydrogen peroxide-, FeCl2/ascorbic acid-, and buthionine sulfoximine (BSO) in primary cortical cultures. We examined the neurotoxicity of eugenol itself in cultures and inhibitory effect of eugenol on NMDA- or kainate (KA)-induced convulsion in BALB/c mice. Each water, methanol extract and methanol fraction of E. caryophyllata was significantly attenuated NMDA-induced delayed neurotoxicity, respectively. Eugenol exhibited a significant inhibitory action against the convulsion evoked by NMDA and KA, and reduced delayed or brief neurotoxicity induced by NMDA, AMPA, and various oxidative injuries. These results suggest that eugenol derived from E. caryophyllata may contribute the neuroprotection against delayed-type excitotoxicity and excitotoxins-mediated convulsion through the amelioration of oxidative stress.

Isoeugenol prevents N-methyl-D-aspartate(NMDA)-induced neurotoxicity and convulsion

Wie, Myung-bok The Korean Society of Veterinary Science 1999 大韓獸醫學會誌 Vol.39 No.2

Isoeugenol, one of the phenylpropanoid derivatives has been known to inhibit the lipid peroxidation via scavenging effect on hydroxyl or superoxide radical production. We examined whether isoeugenol has a inhibitory effect against N-methyl-D-aspartate(NMDA)-, oxygen/glucose deprivation- and xanthine/xanthine oxidase(X/XO)-induced neurotoxicity or NMDA-induced $^{45}Ca^{+2}$ uptake elevation in primary mouse vertical cultures. We also evaluated whether isoeugenol exhibits inhibitory action on NMDA-induced convulsion in mice. Isoeugenol ($30{\sim}300{\mu}M$) attenuated NMDA- and X/XO-induced neurotoxicity by 11~85% and 83~92%, respectively. In the oxyge/glucose deprivation(60 min)-induced neurotoxicity, isoeugenol significantly(p<0.05) reduced by 32% at the maximal concentration. However, it failed to ameliorate NMDA-induced $^{45}Ca^{+2}$ uptake elevation. Isoeugenol(0.5g/kg, i.p.) delayed 6.5 times on the onset time of convulsion evoked by NMDA($0.1{\mu}g$) compared to that of control. These results suggest that the neuroprotective action of isoeugenol may be ascribed to the modulation of massive generation of reactive oxygen species(ROS) occurred during the ischemic or excitotoxic damage, not by directly affecting the NMDA receptor.

Eugenol Inhibits Excitotoxins-Induced Delayed Neurotoxicity, Oxidative Injury and Convulsion

Wie, Myung-Bok,Cheon, Byung-Hwa,Lee, Seon-Young,Son, Kun-Ho,Song, Dong-Keun,Shin, Tae-Kyun,Kim, Hyoung-Chun Korean Society of ToxicologyKorea Environmental Mu 2006 Toxicological Research Vol.22 No.3

In previous our studies, we have reported that eugenol derived from Eugenia caryophyllata(Myrtaceace) exhibits acute N-methyl-D-aspartate(NMDA)- and oxygen/glucose deprivation-induced neurotoxicity in primary cortical cultures and protects hippocampal neurons from global ischemia. In this study, we investigated whether the extracts and fractions of E. caryophyllata or eugenol shows the neuroprotective effects against delayed neuronal injury evoked by NMDA or ${\alpha}$-amino-3-hydroxy-5-methylisoxazole propionate(AMPA), and oxidative damage induced by arachidonic acid-, hydrogen peroxide-, $FeCl_2$/ascorbic acid-, and buthionine sulfoximine(BSO) in primary cortical cultures. We examined the neurotoxicity of eugenol itself in cultures and inhibitory effect of eugenol on NMDA- or kainate(KA)-induced convulsion in BALB/c mice. Each water, methanol extract and methanol fraction of E. caryophyllata was significantly attenuated NMDA-induced delayed neurotoxicity, respectively. Eugenol exhibited a significant inhibitory action against the convulsion evoked by NMDA and KA, and reduced delayed or brief neurotoxicity induced by NMDA, AMPA, and various oxidative injuries. These results suggest that eugenol derived from E. caryophyllata may contribute the neuroprotection against delayed-type excitotoxicity and excitotoxins-mediated convulsion through the amelioration of oxidative stress.

생쥐 피질세포배양에서 Free Radical 유발 신경손상에 대한 손바닥선인장 및 삼백초의 보호효과

위명복(Myung Bok Wie) 대한약학회 2000 약학회지 Vol.44 No.6

The author examined whether the methanol extracts of Opuntia ficus-indica fruit and Saururus chinensis have the inhibitory action on xanthine/xanthine oxidase (X/XO)-, FeCl2/ascorbic acid- and arachidonic acid-induced neurotoxicity in mouse cortical cell cultures. The methanol extracts (10mcg/ml~1mg/ml) of Opuntia ficus-indica and Saururus chinensis were exhibited 53-89% and 48-100% inhibitory action on X/XO-induced neurotoxicity, respectively. At the range of same concentration, both extracts also attenuated the FeCl2/ascorbic acid-induced neurotoxicity by 35-100% and 15-98%, respectively. In arachidoiic acid neurotoxicity, the methanol extracts (1mg/ml) of Opuntia ficus-indica and Saururus chinensis reduced neuronal injury by 22% and 38%, respectively. These results suggest that Opuntia ficus-indica fruit and Saururus chinensis may contribute the neuroprotection in certain free radical-mediated neuronal injury.

Streptozotocin-유발 당뇨쥐의 시상하부에서 Norepinephrine 함량은 정상이나 In vivo Tyrosine Hydroxylase 활성은 감소함

위명복(Myung-Bok Wie),송동근(Dong-Keun Song),강병태(Byung-Tae Kang),정전섭(Jun-Sub Jung),최연식(Yeun-Sik Choi),박준형(Joon-Hyoung Park),김영희(Yung-Hi Kim) 대한약리학회 1991 대한약리학잡지 Vol.27 No.1

Streptozotocin(STZ)-유발 당뇨쥐에서 시상하부의 norepinephrine(NE) 대사를 기초 NE 함량, NE 교체율, in vivo tyrosine hydroxylase(TH) 활성을 그 지표로 하여 조사하였다. STZ (60 mg/kg, iv)로 당뇨를 유발한 후 4주까지 기초 NE 함량은 유의한 변화가 없었다. 그러나 당뇨유발 후 1주째에 측정한 NE 교체율은 대조치의 62%(p<0.01), in vivo TH 활성은 대조치의 34% (p<0.05)로 감소하였다. 이상의 결과로 본 실험에서 측정한 NE 대사의 세 지표 중에서 in vivo TH 활성이 STZ-유발 당뇨쥐의 시상하부 NE 대사의 변화를 가장 민감하게 반영하였다. We studied changes in the hypothalamic norepinephrine(NE) metabolism in streptozotocin (STZ)-induced diabetic rats by measuring basal NE levels, turnover rate of NE, and in vivo tyrosine hydroxylase activities. Basal NE level did not change significantly upto 4 weeks after the establishment of diabetes with STZ(60 mg/kg, iv). But turnover rate of NE decreased to 62% of control rate(P<0.01), and in vivo tyrosine hydroxylase activities decreased to 32% of control level(P<0.05) at one week of diabetes. From these results, we concluded that, of the three parameters measured, in vive tyrosine hydroxylase activity is the most sensitive index of altered hypothalamic NE metabloism in STZ-induced diabetic rats.

Fucoidan attenuates 6-hydroxydopamine-induced neurotoxicity by exerting anti-oxidative and anti-apoptotic actions in SH-SY5Y cells

Kim, Myung-Hwan,Namgoong, Hoon,Jung, Bae-Dong,Kwon, Myung-Sang,Choi, Yeon-Shik,Shin, Taekyun,Kim, Hyoung-Chun,Wie, Myung-Bok The Korean Society of Veterinary Science 2017 大韓獸醫學會誌 Vol.57 No.1

Parkinson's disease (PD) is an irreversible neurological disorder with related locomotor dysfunction and is characterized by the selective loss of nigral neurons. PD can be experimentally induced by 6-hydroxydopamine (6-OHDA). It has been reported that reactive oxygen species, which deplete endogenous glutathione (GSH) levels, may play important roles in the dopaminergic cell death characteristic of PD. Fucoidan, a sulfated algal polysaccharide, exhibits anti-inflammatory and anti-oxidant actions. In this study, we investigated whether fucoidan can protect against 6-OHDA-mediated cytotoxicity in SH-SY5Y cells. Cytotoxicity was evaluated by using MTT and LDH assays. Fucoidan alleviated cell damage evoked by 6-OHDA dose-dependently. Fucoidan reduced the number of apoptotic nuclei and the extent of annexin-V-associated apoptosis, as revealed by DAPI staining and flow cytometry. Elevation of lipid peroxidation and caspase-3/7 activities induced by 6-OHDA was attenuated by fucoidan, which also protected against cytotoxicity evoked by buthionine-sulfoximine-mediated GSH depletion. Reduction in the glutathione/glutathione disulfide ratio induced by 6-OHDA was reversed by fucoidan, which also inhibited 6-OHDA-induced disruption of mitochondrial membrane potential. The results indicate that fucoidan may have protective action against 6-OHDA-mediated neurotoxicity by modulating oxidative injury and apoptosis through GSH depletion.