산발성 장형 위선암 환자의 Microsatellite Instability와 병리학적 양상

조창희,홍유찬,안지현,최경현,이상호,신영명,윤기영,정민정,장희경 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Background : Through many researches, microsatellite is expected to be a good diagnositic and prognostic factor in colorectal cancer, endometrial cancer, gastric cancer, and the others. The prevalence of microsatellite instability (MSI) in gastric carcinoma has reported variously, 13~44%. Purpose : We aimed to determine the prevalence of MSI-high and the relationship between MSI and pathological characteristics of sporadic intestinal type adenocarcinoma of stomach. Material and Methods : We analyzed 106 sporadic intestinal type adenocarcinoma specimens excised from patients who were over thirty-five years old to determine the statue of microsatellite by DNA sequencing. The tissues were formalin-fixed and paraffin embedded. DNA were extracted and amplified by polymerase chain reaction (PCR). MSI was determined using five markers recommended by National Cancer Institute (NCI). Specimens were also studied with five patholical factors-differenciation of tumor cells, depth of invasion, lymph node metastasis, vessel invasion, and perineural invasion- to determine pathological state. Result : The microsatellite statue was determined as MSI-High in 5 cases (4.7%), no MSI-low, and MSS (microsatellite stable) in 101 cases (95.3%). Within the frequency, there was no large gap in the distinction of gender in MSI cases, but in MSS cases, there was three-times more cases in male. MSI cases had moderate-to-poor differenciation and trend to invade toward serosa. All MSI cases showed no perineural invasion. But we could not find any statistical significance between MSI and pathological characteristics of sporadic intestinal type adenocarcinoma. Conclusion : Results suggest that MSI can not make any certain pathological significance in sporadic intestinal type adenocarcinoma. Even though less than 5% of sporadic intestinal type adenocarcinoma patients showed MSI, it can be used as a influential prediagnostic factor of gastric cancer. Further study with large scale of cases will be followed to verify these results.

우측 수신증을 동반한 골반내 방선균증 1예

정명아,서유승,양진수,박준섭,윤진훈,이중건,이준승,이영규,김동희,조성범,주종은 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.2

Pelvic actinomycosis is a chronic granulomatous suppurative disease caused by an anaerobic grampositive organism Actinomyces israelii. It is com-monly associated with an intrauterine device(IUD) and can mimick pelvic or intra-abdominal malignant neoplasm. Ureteral obstruction leading to hydronephrosis is a rare complication of tubo-ovarian abscess. We experienced a case of hydronephrosis as a complication of pelvic actinomycotic abscess. The patient was a 46-year-old women presenting with fever and right flank pain. Leukocytosis and pyuria were present and a hydronephrosis was diagnosed by intravenous pyelography. Ultrasonography and a computerised tomography revealed a mass in right adnexum compressing the right ureter. Removal of retroperitoneal abscess and salphingo-oophorectomy were done and the diagnosis of actinomycosis was made by pathologic finding of resected mass. Postoperatively, the patient was treated with second-generation cephalosporin successfully. (Korean J Nephrol 2002;21(2):337-340)

대장균내에서 발현된 가용성 IL-6의 정제 및 Alanine-Scanning Mutagenesis에 의한 IL-6 Mutant의 제조

이상철,유명희,이민주,변광호,최인표 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.4

Interleukin-6(IL-6) is a multifunctional cytokine that acts on various target cells to induce a diversity of biological responses. Studies were performed on the purification of soluble and functional IL-6 expressed in the periplasm of Escherichia coli. The coding region of the IL-6 gene was fused to the pe1B secretion signal sequence and the expression was under the control of T7 promotor. Upon cell induction under 26 C, recombinant IL-6 was overexpressed in the periplasmic soluble fraction. The periplasmic soluble extract was already 50% pure and was further purified to homogeneity by gel filtration and Mono-Q column chromatography. About 1.5 mg pure protein was obtained from 11 cultured cells. The recombinant IL-6 had a specific activity_ of 1 x 108 U/mg in B9 bioassay, which is eqivalent to that of the natural IL-6. To understand the structure-functional relationship of IL-6, twenty two alanine substitutions of IL-6 have been generated in helix B and surrounding loop regions (amino acid residues 51-109). Most of the mutant proteins were soluble, but those having alanine substitution at Asp7l, G1u93, Phe 94, G1u95, Tyr97, and Arg104 showed little expression or the reduced yield, suggesting that these residues may involve in the folding process or the stability of IL-6 tertiary structure.

Symposia : Women in Life Science ; Government R&D integration for value creation in drug discovery proteomics efforts to target/biomarker discovery

( Myeong Hee Yu ) 한국생화학분자생물학회 (구 한국생화학회) 2007 생화학분자생물학회 춘계학술발표논문집 Vol.2007 No.-

Conformational Properties of Disulfide-Free Recombinant Chicken Ovalbumin

Yu,Myeong-Hee,Jeong,Yeon-Hee The Korea Science and Technology Center 1999 BMB Reports Vol.32 No.3

Chicken egg ovalbumin is a non-inhibitory member of the serpin (serine protease inhibitors) family whose members share a common tertiary fold. In the present study, we succeeded in high-level production of a disulfide-free form of refolded recombinant ovalbumin. Conformational characterization of the recombinant ovalbumim revealed that it is well-folded, following two-state unfolding transition with the midpoint of transition at 4.7 M at 25℃. This value is very close to that of the reduced form of authentic ovalbumin. The recombinant ovalbumin can serve as a model molecule of non-inhibitory serpins in comparative studies with inhibitory members of the serpin family.

Conformational Switch of the Strained Native Serpin Induced by Chemical Cleavage of the Reactive Center Loop

Yu, Myeong Hee,Im, Ha Na 생화학분자생물학회 2001 BMB Reports Vol.33 No.5

The native conformation of serpins (serine protease inhibitors) is strained. Upon cleavage of the reactive center loop of serpins by a protease, the amino terminal portion of the cleaved loop is inserted into the central β-sheet, A sheet, as the fourth strand, with the concomitant release of the native strain. We questioned the role of protease in this conformational switch from the strained native form into a stable relaxed state. Chemical cleavage of the reactive center loop of α₁-antitrypsin, a prototype serpin, using hydroxylamine dramatically increased the stability of the serpin. A circular dichroism spectrum and peptide binding study suggests that the amino terminal portion of the reactive center loop is inserted into the A sheet in the chemically-cleaved α₁-antitrypsin, as in the enzymatically-cleaved molecule. These results indicate that the structural transformation of a serpin molecule does not require interaction with a protease. The results suggest that the serpin conformational switch that occurred during the complex formation with a target protease is induced by the cleavage of the reactive center loop per se.

Conformational Properties of Disulfide-Free Recombinant Chicken Ovalbumin

Yu, Myeong Hee,Jeoung, Yeon Hee 생화학분자생물학회 2000 BMB Reports Vol.32 No.3

Chicken egg ovalbumin is a non-inhibitory member of the serpin (serine protease inhibitors) family whose members share a common tertiary fold. In the present study, we succeeded in high-level production of a disulfide-free form of refolded recombinant ovalbumin. Conformational characterization of the recombinant ovalbumim revealed that it is well-folded, following two-state unfolding transition with the midpoint of transition at 4.7 M at 25℃. This value is very close to that of the reduced form of authentic ovalbumin. The recombinant ovalbumin can serve as a model molecule of non-inhibitory serpins in comparative studies with inhibitory members of the serpin family.

Effect on mRNA Secondary Structure on the Expression Level of β - Galactosidase Fusion Protein

Yu, Myeong Hee,Kim, Chang Soo 한국유전학회 1988 Genes & Genomics Vol.10 No.4

Three recombinant X-lacZ fusion gene was constructed to express the X protein of hepatitis B virus in E. coli. All the fusion genes carried in common the sequence encoding X protein from 10th amino acid residue, which was followed by lacZ in frame. Three fusion genes are different in the region encoding N-terminal portion of the fusion proteins. Plasmid pTXZ1 carried the sequence encoding N-terminal nine residues of X protein so that it had the complete sequence of X ORF. Plasmid pTXZ2 had the polylinker sequence of pUC7 encoding N-terminal seven residues of β-galactosidase at the biginning of the X-lacZ fusion gene. Plasmid pTXZ3 contained both sequences in the order of pUC7 polylinker and the sequence encoding N-terminal nine residues of X protein. E. coli JM109 cells harboring pTXZ1 produced the fusion protein only at a basal level, while cells with pTXZ2 accumulated the fusion protein at a level approaching 20% of total cellular protein. Cells harboring pTXZ3 produced the fusion protein more than that of pTXZ1 but the yield was still much lower than that of pTXZ2. These results indicate that the sequence encoding N-terminal portion of X protein interferes with expression of the fusion protein. Northern blot analysis revealed that expression levels of X-β-galactosidase fusion protein were proportional to the steady state levels of mRNA. Nucleotide sequence encoding N-terminal nine residues of X protein has a potential to form a stem-loop structure which may provide a target site for intracellular RNase attack.

Effect of Amino Acid Substitution at a Site of Temperature Sensitive Folding Mutation of P22 Tailspike Protein

Yu, Myeong Hee,Koh, Hye Yeong,Park, Joo Sang 한국유전학회 1990 Genes & Genomics Vol.12 No.4

Temperature sensitive mutations of the tailspike protein of Salmonella phage P22 affect the folding and chain association at restrictive temperature. Mutant polypeptides which cannot fold and mature properly at 39℃ aggregate inside the cells. Physiological defect of these mutants is likely to be due to the destabilization of an folding intermediate. In order to examine in detail the effect of amino acid substitutions at the mutation sites, twelve different single amino acid substitutions were made at the site of a mutation, TsfU2 (Asp^(238)>Ser). Most of the substitutions except Pro and Arg did not affect folding and maturation of the tailspike protein at 28℃. However, at 39℃ only the wild type residue, Asp, and the Thr substitution allowed the formation of the tailspike trimers. The results suggest that a stereo-specific interaction of the residue 238 is required for the folding and maturation of the tailspike, which is critical for stabilizing the folding intermediate at high temperature.

화아분화기의 야간온도가 절화대국 ‘백마’, ‘진바’의 생육과 절화의 상품성에 미치는 영향

조명환(Myeong Whan Cho),유인호(In Ho Yu),류희룡(Hee Yong You),권준국(Joon Kook Kwon),최경이(Gyeong Lee Choi),강경희(Kyung Hee Kang),홍정희(Jung Hee Hong) 한국원예학회 2009 한국원예학회 학술발표요지 Vol.2009 No.10