Screening and Characterization of Microorganisms with Fibrinolytic Activity from Fermented Foods

(Myeong Ae Yu),(Gwan Sub Sim),(Seung Taek Kwon),(Jae Kwan Hwang),(Jung Kue Shin),(Ik Hyun Yeo),(Yu Rang Pyun),(Seon Joo Yoon) 한국미생물생명공학회 2002 Journal of microbiology and biotechnology Vol.12 No.4

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나노기술응용식품의 국제 연구 및 산업화 동향

유명애(Myeong-Ae Yu) 한국식품과학회 2014 식품과학과 산업 Vol.47 No.1

Effect of a Home-delivered, Protein-rich and Antioxidant-rich Home Meal Replacement on the Dietary Intake and Nutritional Status in Patients Who Have Gastrointestinal Cancer and Are Undergoing Anticancer Treatment

Seon Yu Jung,Woo Jeong Kim,Seung Eun Oh,Mi Ae Kim,Myeong Soo Jeong,Soo Hee Kim,Jin Joo Choi,Joon Seong Park 대한외과학회 2021 대한외과학회 학술대회 초록집 Vol.2021 No.11

Sargassum sp. Attenuates Oxidative Stress and Suppresses Lipid Accumulation in vitro

Jung-Ae Kim(김정애),Fatih Karadeniz,Byul-Nim Ahn(안별님),Myeong Sook Kwon(권명숙),Ok-Ju Mun(문옥주),Mihyang Kim(김미향),Sang-Hyeon Lee(이상현),Ki Hwan Yu(유기환),Yuck Yong Kim(김육용),Chang-Suk Kong(공창숙) 한국생명과학회 2014 생명과학회지 Vol.24 No.3

인간 생체 내 산화스트레스는 조직적 손상을 일으켜 당뇨병, 심장혈관계질환, 비만 등의 대사성 질환의 발병에 관여하는 것으로 알려져 있다. 본 연구에서는 질병예방용 천연 보조제 개발의 일환으로 모자반 3종류(Sargassum hemiphyllum, Sargassum thunbergii, Sargassum honeri)의 에탄올 추출물을 이용하여 항산화활성 및 지방생성억제효과를 비교 검토하였다. 항산화활성은 DPPH 라디칼 소거능, 세포 내 ROS 활성 및 NO 함량의 측정을 통해 검토하였다. 지방세포생성억제활성은 3T3L1세포를 이용하여 지방세포의 축적 정도와 PPARγ 유전자의 발현 정도를 각각 Oil-Red O 염색법과 RT-PCR로 측정하였다. DPPH 라디칼 소거능 결과, 모자반의 처리에 의해 농도 의존적으로 DPPH 라디칼이 줄어드는 경향을 보였으며, 모자반 중에서 S. hemiphyllum 처리군에서 가장 높은 소거 효과를 볼 수 있었다. MTT assay을 통해 모자반의 에탄올 추출물들이 RAW 264.7 cell에 대한 독성을 보이지 않는 농도에서 세포내 실험을 진행하였다. 세포 내 ROS 소거능의 측정 결과, 농도 의존적으로 DCF 형광도 값이 낮게 나왔으며, 시간이 지남에 따라 형광도 값이 일정하여 모자반의 에탄올 추출물에는 세포 내 ROS 생성을 억제하는 효과가 있음을 알 수 있었다. LPS 처리에 의해 증가한 NO 값은 모자반의 추출물들의 처리에 의해 유의적으로 감소하였으며, 감소 정도는 S. hemiphyllum과 S. thunbergii 처리 군에서 높게 나타났다. 모자반의 추출물들을 3T3-L1지방세포에 유도물질과 함께 처리한 결과 모자반 추출물 중 S. hemiphyllum과 S. thunbergii이 세포 내 지방 축적 및 PPAR γ 유전자의 발현을 유의적으로 감소시키는 것으로 나타났다. 이상의 결과로부터 모자반 추출물들 중 S. hemiphyllum과 S. thunbergii이 높은 항산화 활성과 지방세포 생성억제 효능을 보유하고 있는 천연소재임을 확인할 수 있었다. Oxidative stress causes tissue damage and facilitates the progression of metabolic diseases, including diabetes, cardiovascular heart diseases, and obesity. Lipid accumulation and obesity-related complications have been observed in the presence of extensive oxidative stress. As part of an ongoing study to develop therapeutic supplements, Sargassum sp. were tested for their ability to scavenge free radicals and intracellular reactive oxygen species (ROS), as well as to suppress lipid accumulation. Three species, S. hemiphyllum, S. thunbergii, and Sargassum horneri, were shown to scavenge free radicals in a di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) assay. In addition, Sargassum sp. was shown to scavenge intracellular ROS and to decrease nitric oxide (NO) production in H₂O₂ and lipopolysaccharide (LPS)-induced in RAW264.7 mouse macrophages, respectively. Taken together, the results suggest that Sargassum sp. possess huge potential to relieve oxidative stress and related complications, as well as lipid-induced oxidation. They indicate that S. hemiphyllum, S. thunbergii, and S. horneri are potent functional supplements that can produce beneficial health effects through antioxidant and antiobesity activities, with S. hemiphyllum being the most potent among the Sargassum sp. tested. A potential mechanism for the effect of Sargassum sp. on the suppression of lipid accumulation in differentiating 3T3-L1 mouse preadipocytes through deactivation of the peroxisome proliferator-activated receptor γ (PPAR γ) is presented.

Comparison of Functional Constituents and Biological Activity of the Seed Extracts from Two Mulberry Fruits

Eun-Ok Kim,Myeong-Hwa Yu,Yu-Jin Lee,Hyun-Hee Leem,Shin-Ae Kim,Dae-Hun Kang,Sang-Won Choi 한국식품영양과학회 2010 Preventive Nutrition and Food Science Vol.15 No.2

The seeds from two mulberry fruits [Morus alba (MA) and Cudrania tricuspidata (CT)] were examined for their oil content, and fatty acid, phytosterol and tocopherol compositions and contents. Moreover, polyphenolic compounds and biological activity of the two defatted seed residue extracts were also evaluated. Oil contents of MA and CT seeds were 29.36% and 16.69%, respectively, while MeOH extracts of the defatted MA and CT seed residues were 5.10% and 6.22%, respectively. The two seed oils were composed of 81.4 and 74.37% linoleic, 5.75 and 11.39% oleic, 8.40 and 10.18% palmitic acid, and 3.52 and 3.0% stearic acids, and two other minor fatty acids, such as linolenic and arachidic acids. MA seed had higher contents of phytosterols (507.59 ㎎/100 g of oil), tocopherols (99.64 ㎎/100 g of oil), and total flavonoid (106.50 ㎎/100 g of seed) than CT seed, whereas CT seed had higher levels of total polyphenol than MA seed. The MeOH extract of MA seed residue showed higher antioxidant, anti-diabetic, and anti-melanogenic activity than that of CT seed residue. trans-Resveratrol (9.62 ㎎/100 g), quercetin (54.83 ㎎/100 g), and 4-prenylmoracin (48.70 ㎎/100 g), were found to be the main polyphenolic components in the MeOH extract of MA seed residue. These results indicate that MA seeds are good sources of essential dietary phytochemicals with antioxidant, anti-diabetic and anti-melanogenic activity.

Comparison of Functional Constituents and Biological Activity of the Seed Extracts from Two Mulberry Fruits

Kim, Eun-Ok,Yu, Myeong-Hwa,Lee, Yu-Jin,Leem, Hyun-Hee,Kim, Shin-Ae,Kang, Dae-Hun,Choi, Sang-Won The Korean Society of Food Science and Nutrition 2010 Preventive Nutrition and Food Science Vol.15 No.2

The seeds from two mulberry fruits [Morus alba (MA) and Cudrania tricuspidata (CT)] were examined for their oil content, and fatty acid, phytosterol and tocopherol compositions and contents. Moreover, polyphenolic compounds and biological activity of the two defatted seed residue extracts were also evaluated. Oil contents of MA and CT seeds were 29.36% and 16.69%, respectively, while MeOH extracts of the defatted MA and CT seed residues were 5.10% and 6.22%, respectively. The two seed oils were composed of 81.4 and 74.37% linoleic, 5.75 and 11.39% oleic, 8.40 and 10.18% palmitic acid, and 3.52 and 3.0% stearic acids, and two other minor fatty acids, such as linolenic and arachidic acids. MA seed had higher contents of phytosterols (507.59 mg/100 g of oil), tocopherols (99.64 mg/100 g of oil), and total flavonoid (106.50 mg/100 g of seed) than CT seed, whereas CT seed had higher levels of total polyphenol than MA seed. The MeOH extract of MA seed residue showed higher antioxidant, anti-diabetic, and anti-melanogenic activity than that of CT seed residue. trans-Resveratrol (9.62 mg/100 g), quercetin (54.83 mg/100 g), and 4-prenylmoracin (48.70 mg/100 g), were found to be the main polyphenolic components in the MeOH extract of MA seed residue. These results indicate that MA seeds are good sources of essential dietary phytochemicals with antioxidant, anti-diabetic and anti-melanogenic activity.

Molecular Cloning and Characterization of a cDNA Encoding Caprine β - Lactoglobulin

Kim, Ji Young,Yu, Myeong Hee,Kim, Ae Kyeong,Kim, Jae Man 한국유전학회 1993 Genes & Genomics Vol.15 No.2

The complete sequence of caprine β-lactoglobulin cDNA has been determined. The caprine β-lactoglobulin cDNA consists of 540 nucleotide coding region, flanked by 40 nucleotide 5'-untranslated region (UTR) and 207 nucleotide 3'-UTR which was followed by 78 nucleotide polyadenylation. The coding region encodes 180 amino acids which consist of l8 amino acid-long signal peptide and 162 amino acid-long mature β-lactoglobulin. The caprine β-lactoglobulin cDNA differs from that of its ovine counterpart by 7 nucleotides in the protein coding sequence resulting in 2 amino acid changes at amino acid positions -3 and +130. The nucleotide sequence at 5' -UTR and coding region was highly conserved with respect to the ovine β-lactoglobulin and was more homologous to ovine cDNA (99%) than the bovine cDNA (94%). However, the 3' -UTR of the caprine β-lactoglobulin mRNA was less conserved and it is more homologous to bovine cDNA (94%) than that of the ovine mRNA (90%). The sequence analysis revealed that the β-lactoglobulin molecules are highly conserved during the evolution of ruminant species.

Root-knot nematode ( Meloidogyne incognita ) control using a combination of Lactiplantibacillus plantarum WiKim0090 and copper sulfate

Kim Seulbi,Kim Ho Myeong,Seo Hye Jeong,Yeon Jehyeong,Park Ae Ran,Yu Nan Hee,Jeong Seul-Gi,장지윤,김진철,박해웅 한국미생물·생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.8

Lactic acid bacteria (LAB) exert antagonistic activity against root-knot nematodes, mainly by producing organic acids via carbohydrate fermentation. However, they have not yet been used for root-knot nematode (Meloidogyne incognita) control owing to a lack of economic feasibility and effectiveness. In this study, we aimed to isolate organic acid-producing LAB from kimchi (Korean traditional fermented cabbage) and evaluated their nematicidal activity. Among the 234 strains isolated, those showing the highest nematicidal activity were selected and identified as Lactiplantibacillus plantarum WiKim0090. Nematicidal activity and egg hatch inhibitory activity of WiKim0090 culture filtrate were dose dependent. Nematode mortality 3 days after treatment with 2.5% of the culture filtrate was 100%, with a 50% lethal concentration of 1.41%. In pot tests, the inhibitory activity of an L. plantarum WiKim0090-copper sulfate mixture on gall formation increased. Compared to abamectin application, which is a commercial nematicide, a higher control value was observed using the WiKim0090-copper sulfate mixture, indicating that this combination can be effective in controlling the root-knot nematode.

Therapeutic effects of <i>Echinococcus granulosus</i> cystic fluid on allergic airway inflammation

Kim, Hye-Jin,Kang, Shin-Ae,Yong, Tai-Soon,Shin, Myeong-Heon,Lee, Kyu-Jae,Park, Gab-Man,Suvonkulov, Uktamjon,Yu, Hak Sun Elsevier 2019 Experimental parasitology Vol.198 No.-

<P><B>Abstract</B></P> <P>Previous studies showed that <I>Echinococcus granulosus</I> infection reduces allergic airway inflammation in experimentally infected hosts and the cystic fluid of <I>E. granulosus</I> is known to activate regulatory T (CD4<SUP>+</SUP>CD25<SUP>+</SUP>Foxp3<SUP>+</SUP>T, Treg) cells. To evaluate the effects of cystic fluid of <I>E. granulosus</I> on allergic airway inflammation, we investigated the regulation of the inflammatory reaction by cystic fluid using an allergic airway inflammation animal model. Cystic fluid was administered to C57BL/6 mice seven times every other day, after which allergic airway inflammation was induced using ovalbumin and aluminum. The airway resistance, number of eosinophils and other immune cells in the bronchoalveolar lavage fluid, and levels of Th2 and Th17-related cytokines were significantly reduced by cystic fluid pre-treatment in allergic airway inflammation-induced mice. The number IL-4<SUP>+</SUP>CD4<SUP>+</SUP> T cells decreased, the number of Treg cells increased in the lung-draining lymph nodes and spleen of cystic fluid pre-treated mice. In conclusion, <I>E. granulosus</I>-derived cystic fluid may alleviate the Th2 allergic airway inflammatory response via Treg cells. Further studies of the immune regulation of cystic fluid may lead to the development of therapeutic agents for immune disorders.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The regulation of the inflammatory reaction by cystic fluid was studied using an allergic airway inflammation animal model. </LI> <LI> Cystic fluid treatment significantly reduced the airway resistance and eosinophils in the bronchoalveolar lavage fluid. </LI> <LI> The number IL-4<SUP>+</SUP>CD4<SUP>+</SUP> T cells decreased in the lung-draining lymph nodes and spleen of cystic fluid pre-treated mice. </LI> <LI> <I>E. granulosus</I>-derived cystic fluid may alleviate the Th2 allergic airway inflammatory response via Treg cells. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

간호사 경력개발제도에 관한 연구

권인각,성영희,박광옥,유옥수,김명애 병원간호사회 2007 임상간호연구 Vol.13 No.1

Purpose: This study was aimed to develop a desirable clinical ladder system(CLS) model for hospital nurses in order to make recommendations for CLS application in Korea. Methods: This study was carried out through literature review and a survey of the state of CLS application in Korea. 134 hospital nurses who attended the seminar on CLS. participated in the survey. Results: 1. Most of hospital nurses have positive attitude toward the introduction of CLS and perceived the CLS would promote excellence in clinical nursing and improve nurses' job satisfaction. 2. To establish a good CLS model, a proper number of stages must be established and the level of excellence in each stage should be clearly delineated. The most preferred one was four-stage model. 3. For evaluation methods for promotion, test, performance evaluation, portfolios, and exemplars were suggested and would be used in combination. However, maintaining fairness and staff nurses' workload and psychological burden challenge the CLS application. 4. For compensation, financial reward was mostly preferred, and chances for personal development and professional compensation, compensation in working conditions and social recognition were proved to be useful. 5. To be successful, excessive stress, sense of incongruity, competitive atmosphere, concerns about unfairness, and despair or withdrawal after failure in promotion or reappointment should be considered and overcome. Conclusion: Some recommendations for introducing CLS were made. In order to suggest an ideal CLS model in Korea, more comprehensive survey and outcome analysis of the CLS are needed.