재래종 콩 유전자원의 주요 형태적 특성과 변이

윤문섭,백형진,이정란,김행훈,조양희,안종웅,김창영 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.4

본 실험은 한국, 중국 및 일본 재래종 콩 유전자 자원의 주요형질 변이를 조사하고 이들 자원간의 지리적 유연관계를 비교하고자 수행하였으며, 그 결과를 요약하면 다음과 같다. 1. 경장은 한국(66㎝)이나 일본자원(54.9㎝)보다 중국자원(71㎝)에서 더 컸으며, 또한 변이도 중국자원에서 가장 큰 것으로 나타났다. 2. 개화일수 및 생육일수는 한국자원(65.5일, 151.4일)이 가장 길었으며 중국(57일, 120일)과 일본자원(59.9일, 122.4일)은 같은 경향을 나타냈다. 또한 한국과 중국자원은 지역에 따른 차이를 나타냈으나 일본자원에서는 확인할 수 없었다. 3. 백립중은 한국(18.8g), 일본(17.2g) 및 중국(14.7g) 순으로 무거웠다. 특히 한국자원은 9~11g과 23~25g에서 정점을 나타냈고, 변이폭도 가장 컸다. 반면 일본과 중국자원은 13~17g사이에서 정점을 나타냈다. 4. 협수는 중국(46.9개)이나 일본자원(36.5개)보다 한국자원(75.5개)에서 가장 많았으며, 변이폭도 한국자원에서 가장 컸다. 내도복성은 일본자원에서 가장 강하였다. 5. 정준판별분석결과 can 1을 경계로 하여 오른쪽 상하에 한국재래종이 주로 분포하였고 왼쪽에는 중국과 일본 재래종이 주로 분포하여 각 나라별로 고유한 영역을 가지고 분리되었다. 전체적 경향이 중국과 일본자원이 한국자원보다 가까운 경향을 나타냈다. This experiment was carried out to compare the characters of 944 Korean soybean landraces, 716 Chinese, and 170 Japanese soybean landraces introduced from USDA soybean germplasm collection. Cannonical discriminant and cluster analyses were conducted by their origins. The population from China was taller in plant height than those from Korea and Japan, also its variation was largest in Chinese population. The population from Korea was langer than those from China and Japan in terms of days to flowering and maturity. Also, Korean and Chinese accessions appeared the difference of those traits according to latitude. One hundred seeds weight was in the order of Korean(18.8g), Japanese(17.2g) and Chinese populations(14.7g), especially Korean population was distinguished into two groups; the group including accessions between 10 and 15g, and the other group including accessions between 20 and 25g. However, others showed the peak in range of 13~17g. The number of pod per plant was more in Korean accession(75.5) with the largest variation than Chinese(46.9) and Japanese accession(36.5). The distribution of Korean, Chinese and Japanese accessions reflected the origin of accessions analyzed by cannonical discriminant analysis. The relationship between their geographical origins showed the population of China and Japan are losely related.

일본 Okinawa지역에서 두류유전자원의 수집과 Vigna riukiuensis의 지리적 변이

윤문섭,Kaga, Akito,Tomooka, Norihiko,Vaughan, Duncan,백형진,김창영 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.4

일본 남서제도에 자생하는 두류 근연야생종집단의 분포와 집단구조를 파악하기 위해서 1999년 10월 21일부터 29일 까지 오끼나와 지역의 Hateruma, Tarama, Yonaguni 섬들에서 탐색ㆍ수집을 수향한 결과와 이들 섬으로부터 수집된 Vigna riukiuensis 집단에 대한 AFLP분석을 통하여 그들의 지리적 변이를 분석한 결과는 다음과 같다. 1. 일본의 본도에서는 발견되지 않는 Vigna riukiuensis, V. reflexo-pilosa, V. luteola, V. minima, Canavalia rosea 및 Glucine tabacina와 같은 야생종을 수집하였으며, 또한 재배종인 Macroptilium atropureum, Vigna unguiculate 및 Glycine max를 수집하였다. 2. V. riukiuensis는 잔디밭에서만 발견되었고, V. marina와 C. rosea는 해안가의 모래사장에서 주로 발견되어 두류의 내염성 육종에 유용한 재료가 될 것으로 기대되었다. 서식집단의 범위는 10여 개체에서부터 1㏊에 이르기까지 매우 다양했다. G. tabacina는 Miyako의 바닷가와 인접한 잔디밭에서만 발견되었으며, 사료작물로 도입된 M. atropureum은 대부분의 섬에서 반 재배형으로 널리 분포하고 있었다. 3. 수집된 자원은 총 74점으로서 V. riukiuensis 27점, V. reflexo-pilosa 10점, V. marina 17점, V. luteola 2점, V. unguiculate 5점, G. tabacina 5점, G. max 1점, M. atropureum 3점 및 C. rosea 4점을 각각 포함했다. 또한 이들 자원에 대한 근류균의 수집과 식물표본이 만들어 졌으며, 각 수집지점의 위치가 기록되었다. 4. V. riukiuensis집단들 사이의 지리적 유연관계는 principal coordinate analysis(PCA)에 의해 처음 두개의 주요성분은 전체변이의 57.9%를 설명했다. 전체 변이의 46.0%를 설명하는 PCA1은 Yonaguni-Ishigaki-Iromote-Hateruma집단과 Miyakowlqeks으로 구분시켰다. 전체변이의 11.9%를 설명하는 PCA2에 의해 Haterumawlqeks은 Yonaguni-Ishigaki-Iromote집단과 분리되었다. Exploration and collection of legume genetic resources was conducted on the southern islands of Okinawa prefecture, Hateruma, Iriomote, Irabu, Ishigaki, Miyako, Tarama and Yonaguni islands, Japean, from 21^(st) to 29^(th) October. 1999. From these islands, the following wild species were collected Vigna riukiuensis, V, reflexo-pilosa, V. luteola, V. marina, Canavalia rosea and Glycine tabacina and seeds or root nodules of the following cultivated species Macroptilium atropurpureum. Vigna unguicullata and Glycine max. The wild species collected were generally found in open herbaceous habitats such as roadside bushes, lawns or on beaches. Several species have specific adaptations such as V. riukiuensis and G. tabacina whic can survive in cut lawns. V. marina and C. rosea grow along sandy beaches of every island visited and may have physiological tolerances, such as salt tolerance, which may be of value in legume breeding. Population size varied greatly within and between species from less than 10 plants to covering more than 1ha. M. atropurpureum, introduced as a forage crop, has escaped and is widely changing. During this exploration and collection, V. riukiuensis and V. reflexo-pilosa , from the subgenus Ceratotropis were both found on Ishigaki, Iriomote, Yonaguni and Miyako islands. On the small island of Irabu and Tarama only V. reflexo-pilosa from the subgenus Ceratotropis was found. On Hateruma only V. riukiuensis from subgenus Ceratotropis was found. A total of 74 wild legume seed samples were collected. The comprehensive collection will enable diversity analysis to highlight islands and populations of particular genetic diversity for conservation and evaluation. Geographical variation of V. riukiuensis among the collected species was found within it's populations by AFLP analysis. Populations of Miyako island were genetically different from of other islands of Okinawa based on PCA.

Phosphorescence Color Tuning of Cyclometalated Iridium Complexes by <i>o</i>-Carborane Substitution

Kim, Taewon,Kim, Hyungjun,Lee, Kang Mun,Lee, Yoon Sup,Lee, Min Hyung American Chemical Society 2013 Inorganic Chemistry Vol.52 No.1

<P>Heteroleptic (<I>C</I><SUP>∧</SUP><I>N</I>)<SUB>2</SUB>Ir(acac) (<I>C</I><SUP>∧</SUP><I>N</I> = 4-<I>CBppy</I> (<B>1</B>); 5-<I>CBppy</I> (<B>2</B>), 4-<I>fppy</I> (<B>4</B>) <I>CB</I> = <I>ortho</I>-methylcarborane; <I>ppy</I> = 2-phenylpyridinato-C<SUP>2</SUP>,N, 4-<I>fppy =</I> 2-(4-fluorophenyl)pyridinato-C<SUP>2</SUP>,N, acac = acetylacetonate) complexes were prepared and characterized. While <B>1</B> exhibits a phosphorescence band centered at 531 nm, which is red-shifted compared to that of unsubstituted (<I>ppy</I>)<SUB>2</SUB>Ir(acac) (<B>3</B>) (λ<SUB>em</SUB> = 516 nm), the emission spectrum of <B>2</B> shows a blue-shifted band at 503 nm. Comparison with the emission band for the 4-fluoro-substituted <B>4</B> (λ<SUB>em</SUB> = 493 nm) indicates a substantial bathochromic shift in <B>1</B>. Electrochemical and theoretical studies suggest that while carborane substitution on the 4-position of the phenyl ring lowers the <SUP>3</SUP>MLCT energy by a large contribution to lowest unoccupied molecular orbital (LUMO) delocalization, which in turn assigns the lowest triplet state of <B>1</B> as [d<SUB>π</SUB>(Ir)→π*(<I>C</I><SUP>∧</SUP><I>N</I>)] <SUP>3</SUP>MLCT in character, the substitution on the 5-position raises the <SUP>3</SUP>MLCT energy by the effective stabilization of the highest occupied molecular orbital (HOMO) level because of the strong inductive effect of carborane. An electroluminescent device incorporating <B>1</B> as an emitter displayed overall good performance in terms of external quantum efficiency (6.6%) and power efficiency (10.7 lm/W) with green phosphorescence.</P><P>The introduction of an <I>o</I>-carborane to the 4- and 5-position of the phenyl ring of a <I>ppy</I> ligand in heteroleptic (<I>C</I><SUP>∧</SUP><I>N</I>)<SUB>2</SUB>Ir(acac) complexes gave rise to red and blue shifts of the phosphorescence band, respectively, compared to that of (<I>ppy</I>)<SUB>2</SUB>Ir(acac). PhOLED devices incorporating the 4-carborane substituted Ir(III) complex as a phosphorescent emitter displayed good performance with green phosphorescence.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/inocaj/2013/inocaj.2013.52.issue-1/ic3015699/production/images/medium/ic-2012-015699_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ic3015699'>ACS Electronic Supporting Info</A></P>

Deep Red Phosphorescence of Cyclometalated Iridium Complexes by <i>o</i>-Carborane Substitution

Bae, Hye Jin,Chung, Jin,Kim, Hyungjun,Park, Jihyun,Lee, Kang Mun,Koh, Tae-Wook,Lee, Yoon Sup,Yoo, Seunghyup,Do, Youngkyu,Lee, Min Hyung American Chemical Society 2014 Inorganic chemistry Vol.53 No.1

<P>Heteroleptic (C<SUP>∧</SUP>N)<SUB>2</SUB>Ir(acac) (C<SUP>∧</SUP>N = 5-MeCBbtp (<B>5a</B>); 4-BuCBbtp (<B>5b</B>); 5-BuCBbtp (<B>5c</B>); 5-(<I>R</I>)CBbtp = 2-(2′-benzothienyl)-5-(2-<I>R</I>-<I>ortho</I>-carboran-1-yl)-pyridinato-C<SUP>2</SUP>,N, R = Me and <I>n</I>-Bu; 4-BuCBbtp = 2-(2′-benzothienyl)-4-(2-<I>n</I>-Bu-<I>ortho</I>-carboran-1-yl)-pyridinato-C<SUP>2</SUP>,N, acac = acetylacetonate) complexes supported by <I>o</I>-carborane substituted C<SUP>∧</SUP>N-chelating ligand were prepared, and the crystal structures of <B>5a</B> and <B>5b</B> were determined by X-ray diffraction. While <B>5a</B> and <B>5c</B> exhibit a deep red phosphorescence band centered at 644 nm, which is substantially red-shifted compared to that of unsubstituted (btp)<SUB>2</SUB>Ir(acac) (<B>6</B>) (λ<SUB>em</SUB> = 612 nm), <B>5b</B> is nonemissive in THF solution at room temperature. In contrast, all complexes are emissive at 77 K and in the solid state. Electrochemical and theoretical studies suggest that the carborane substitution leads to the lowering of both the HOMO and LUMO levels, but has higher impact on the LUMO stabilization than the HOMO, resulting in the reduction of the triplet excited state energy. In particular, the LUMO stabilization in the 4-substituted <B>5b</B> is more contributed by carborane than that in the 5-substituted <B>5a</B>. The solution-processed electroluminescent device incorporating <B>5a</B> as an emitter displayed deep red phosphorescence (CIE coordinate: 0.693, 0.290) with moderate performance (max η<SUB>EQE</SUB> = 3.8%) whereas the device incorporating <B>5b</B> showed poor performance, as well as weak luminance.</P><P>The introduction of an <I>o</I>-carborane to the 4- or 5-position of the pyridine ring of a btp ligand in heteroleptic (C<SUP>∧</SUP>N)<SUB>2</SUB>Ir(acac) complexes leads to deep red phosphorescence, which is substantially red-shifted compared to that of (btp)<SUB>2</SUB>Ir(acac). The solution processed PhOLED devices incorporating the 5-carborane substituted Ir(III) complex as an emitter display moderate performance with deep red phosphorescence.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/inocaj/2014/inocaj.2014.53.issue-1/ic401755m/production/images/medium/ic-2013-01755m_0010.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ic401755m'>ACS Electronic Supporting Info</A></P>

Novel Reversible Zn²⁺-Assisted Biological Phosphate “Turn-On” Probing through Stable Aryl-hydrazone Salicylaldimine Conjugation That Attenuates Ligand Hydrolysis

Tsay, Olga G.,Manjare, Sudesh T.,Kim, Hyungjun,Lee, Kang Mun,Lee, Yoon Sup,Churchill, David G. American Chemical Society 2013 Inorganic Chemistry Vol.52 No.17

<P>A novel reversible zinc(II) chemosensing ensemble (<B>2</B>·Zn<SUP>2+</SUP>) allows for selective “turn-on” fluorescence sensing of ATP and PPi in aqueous media (detection limits: 2.4 and 1.0 μM, respectively) giving selective binding patterns: ATP ∼ PPi > ADP ≫ AMP > monophosphates ≈ remaining ions tested. The conjugated hydrazone [CN<I>NH</I>R] resists hydrolysis considerably, compared to the imine [CN<I>CH</I><SUB><I>2</I></SUB>R, pyridin-2-ylmethanamine] functionality, and generalizes to other chemosensing efforts. Prerequisite Zn<SUP>2+</SUP>·[O<SUB>phenol</SUB>N<SUB>imine</SUB>N<SUB>pyr</SUB>] binding is selective, as determined by UV–vis and NMR spectroscopy; ATP or PPi extracts Zn<SUP>2+</SUP> to regenerate the ligand–fluorophore conjugate (PPi: turn-on, 512 nm; detection limit, 1.0 μM). Crystallography, 2-D NMR spectroscopy, and DFT determinations (B3LYP/631g*) support the nature of compound <B>2</B>. 2-Hydrazinyl-pyridine-salicylaldehyde conjugation is unknown, as such; a paucity of chemosensing-Zn<SUP>2+</SUP> binding reports underscores the novelty of this modifiable dual cation/anion detection platform. A combined theoretical and experimental approach reported here allows us to determine both the potential uniqueness as well as drawbacks of this novel conjugation.</P><P>A chemosensing ensemble, involving conjugated hydrazone [CN<I>NH</I>R], greatly resists hydrolysis to allow for reversible and selective “turn-on” fluorescence sensing of ATP and PPi in aqueous media: ATP ∼ PPi > ADP ≫ AMP > monophosphates ≈ others.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/inocaj/2013/inocaj.2013.52.issue-17/ic4013526/production/images/medium/ic-2013-013526_0011.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ic4013526'>ACS Electronic Supporting Info</A></P>

Turning On MLCT Phosphorescenceof Iridium(III)–BoraneConjugates upon Fluoride Binding

Vadavi, RameshS.,Kim, Hyungjun,Lee, Kang Mun,Kim, Taewon,Lee, Junseong,Lee, Yoon Sup,Lee, Min Hyung American ChemicalSociety 2012 Organometallics Vol.31 No.1

<P>The heteroleptic complexes (<I>C</I><SUP>∧</SUP><I>N</I>)<SUB>2</SUB>Ir<SUP>III</SUP> (<I>C</I><SUP>∧</SUP><I>N</I>: ppy, <B>2</B>; btp, <B>3</B>), bearing a 4-(dimesitylboryl)benzoate (Bbz) auxiliary ligand,and their fluoride adducts were prepared and characterized. Whilethe PL spectra of <B>2</B> and <B>3</B> exhibit very weakemission in solution, the addition of fluoride turned on (<I>C</I><SUP>∧</SUP><I>N</I>)<SUB>2</SUB>Ir-centeredphosphorescence (λ<SUB>em</SUB> 527 nm for <B>2</B> and617 nm for <B>3</B>). Experimental and DFT calculations suggestthat the weak emission is ascribable to a photoinduced electron transferfrom the MLCT state of the (<I>C</I><SUP>∧</SUP><I>N</I>)<SUB>2</SUB>Ir moiety to the Bbz ligand and is switchedon by fluoride binding to the boron center of the Bbz ligand.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/orgnd7/2012/orgnd7.2012.31.issue-1/om2008937/production/images/medium/om-2011-008937_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/om2008937'>ACS Electronic Supporting Info</A></P>

Turn-on colorimetric sensing of fluoride ions by a cationic triarylborane bearing benzothiazolium

Song, Ki Cheol,Lee, Kang Mun,Kim, Hyungjun,Lee, Yoon Sup,Lee, Min Hyung,Do, Youngkyu Elsevier 2012 Journal of organometallic chemistry Vol.713 No.-

<P><B>Abstract</B></P><P>A cationic triarylborane Lewis acid bearing benzothiazolium moiety, 2-(4′-dimesitylborylphenyl)-3-methylbenzo[<I>d</I>]thiazol-3-ium ([<B>2</B>]<SUP>+</SUP>) was prepared from a neutral borane (<B>2a</B>) and their crystal structures were determined from X-ray diffraction studies. While <B>2a</B> shows a blue-shift with a small decrease in the UV–vis absorption band upon fluoride binding to the boron atom, [<B>2</B>]<SUP>+</SUP> undergoes a red-shift of the absorption band which tails over visible region, giving rise to the color change of the solution from colorless to yellow. The fluoride binding constant in THF/H<SUB>2</SUB>O (9/1, v/v) is calculated to be 1.3 × 10<SUP>4</SUP> M<SUP>−1</SUP> that is much greater than that of a neutral borane <B>2a</B> (<I>K</I> = 4.2 × 10<SUP>2</SUP> M<SUP>−1</SUP>). DFT calculation results suggest that the absorption process in the fluoroborate (<B>2</B>F) is involved with π(Mes)→π*(phenylbenzothiazolium) intramolecular charge transfer and the greater elevation of the π(Mes) donor level is responsible for the turn-on colorimetric response of [<B>2</B>]<SUP>+</SUP>.</P> <P><B>Graphical abstract</B></P><P>A cationic triarylborane Lewis acid bearing benzothiazolium moiety ([<B>2</B>]<SUP>+</SUP>) was prepared and characterized. [<B>2</B>]<SUP>+</SUP> displays enhanced fluoride ion affinity and turn-on colorimetric response toward fluoride.<ce:figure id='dfig1'></ce:figure></P><P><B>Highlights</B></P><P>► A novel cationic triarylborane Lewis acid was synthesized. ► Fluoride ion affinity was significantly enhanced. ► Fluoride binding led to turn-on color change from colorless to yellow.</P>

Genotypic and Geographical Variations of $\beta$amylase Isozyme in Soybean Land Races by Isoelectric Focusing (IEF)

Yoon, Mun-Sup,Ahn, Jong-Woong,Kang, Jung-Hoon,Baek, Hyung-Jin,Park, Nam-Kyu,Rho, Yong-Deok The Korean Society of Crop Science 2000 Korean journal of crop science Vol.45 No.2

The experiment was carried out to study the variations and geographical distribution of $\beta$-amylase isozyme by isoelectric focusing (IEF) within Korean, Chinese and Japanese soybean land races. In pH 3-10 gel of IEF, the amylase of soybean accessions was separated into low pI group isozymes (${$Sp_1$}^b$) and high pI group isozymes(${$Sp_1$}^a$). In pH4-6.5 gel, isoelectric points were at 5.07, 5.15, 5.25, 5.40, and 5.94, and h, j, and k bands also were found. The distribution of Sp$_1$$^{a}$ allele (high pI type) was 29.3% in soybean accessions from Korea, 10.1 % in those from China, and 6.9% in Japanese accessions. The percentage of ${$Sp_1$}^a$) allele was the highest in soybean accessions from Kyungsang province (35 %) in Korea, then central China (32 %) in China, and Honshu (10%) in Japan.

Geographical Patterns of Morphological Variation in Soybean Germplasm

Yoon, Mun-Sup,Ahn, Jong-Woong,Park, Sei-Joon,Baek, Hyung-Jin,Park, Nam-Kyu,Rho, Young-Deok The Korean Society of Crop Science 2000 Korean journal of crop science Vol.45 No.4

A total of 1,830 soybean collections were grown in the field and characterized for 10 morphological traits to determine the diversity and relationship within and among geographical regions. Phenotypic variation was found within all regions for most characters. The Shannon-Weaver diversity index ranged from 0.49 to 0.62 across regions, and 0.09 to 1.00 across characters. Canonical discriminant analysis and clustering of the canonical means delineated 3 regional clusters: (ⅰ) Kyunggi, Chungchong, Kangwon, Chulla, and Kyungsang; (ⅱ) Heilongjiang; and (ⅲ) Jilin, Manchuria, central China, south China, Others (China), Hokkaido, Honshu, and Others (Japan).

Genotypic and Geographical Variations of β-amylase Isozyme in Soybean Land Races by Isoelectric Focusing (IEF)

Mun Sup Yoon,Jong Woong Ahn,Jung Hoon Kang,Hyung Jin Baek,Nam Kyu Park,Young Deok Rho 韓國作物學會 2000 Korean journal of crop science Vol.45 No.2

The experiment was carried out to study the variations and geographical distribution of β -amylase isozyme by isoelectric focusing (IEF) within Korean, Chinese and Japanese soybean land races. In pH 3-10 gel of IEF, the amylase of soybean accessions was separated into low pI group isozymes (TEX>Sp1 b) and high pI group isozymes(Sp1 a). In pH4-6.5 gel, isoelectric points were at 5.07, 5.15, 5.25, 5.40, and 5.94, and h, j, and k bands also were found. The distribution of Sp1 a allele (high pI type) was 29.3% in soybean accessions from Korea, 10.1 % in those from China, and 6.9% in Japanese accessions. The percentage of Sp1 a) allele was the highest in soybean accessions from Kyungsang province (35 %) in Korea, then central China (32 %) in China, and Honshu (10%) in Japan