Binding of Galectin-1 to Neutrophils Enhanced by Activation

Cho, Somi K.,Cho, Moonjae 한국응용생명화학회 2000 Journal of Applied Biological Chemistry (J. Appl. Vol.43 No.3

Human polymorphonuclear neutrophils undergo diaphoresis after a selectin-mediated rolling on the endothelial cells of the blood vessel wall. Extravasation is believed to be an integrin-mediated process. Galectin-1 is a small dimeric beta-galactoside-binding protein synthesized by the endotherial cells and present in the perivascular connective tissue. In this study we suggest the possible role of galectin-1 in extravasation of the activated neutrophils. MAL lectin binding study showed, that f-MetLeuPhe-activated neutrophils decrease surface sialylation and increase galectin-1 binding via exposure of new galectin-1 binding sites. Desialylated HL-60 cells also show the same decrease in MAL binding and increase in galectin-1 binding, an increase which was not observed in the presence of lactose. Galectin-1 blotting analysis detected two possible major ligands (approximately 120 and 160 kDa) of galectin-1 from the desialylated HL-60 cell lysates.

Chronic Granulomatous Disease on Jeju Island, Korea

Moonjae Cho,Kyung-Sue Shin 대한의학유전학회 2013 대한의학유전학회지 Vol.10 No.1

Chronic granulomatous disease (CGD) is a rare inherited disorder of a defective NADPH oxidase enzyme, resulting in very low or no production of superoxide and subsequent reactive oxygen species. Consequently, patients with CGD are highly susceptible to severe bacterial and fungal infections. CGD is a genetically heterogeneous disease caused by defects in any one of the genes encoding the NADPH oxidase components. CGD generally affects about 3-4 per 1,000,000 individuals; thus, it is surprising that the prevalence of CGD on Jeju Island is 34.3 per 1,000,000 individuals. At present, 20 patients with CGD from 14 unrelated families on Jeju Island have been identified; nine males and 11 females. All patients with CGD tested on Jeju Island had an identical and homozygous mutation (c.7C>T in CYBA, p.Q3X in p22phox). Therefore, all patients were autosomal recessive form of CGD. This strongly suggests that the unique and identical mutation in CYBA may be inherited from a common proband. Using mutation-specific primers to detect the mutated allele in CYBA, the frequency of subjects carrying a mutated allele was 1.3% of enrolled subjects from Seogwipo City. Further studies are necessary to elucidate how frequently this mutant allele occurs in the population on Jeju Island. Additionally, it is important to construct a national registry system to understand the pathophysiology of CGD and develop a strategy for long-term therapy.

Chronic Granulomatous Disease on Jeju Island, Korea

Cho, Moonjae,Shin, Kyung-Sue Korean Society of Medical Genetics and Genomics 2013 대한의학유전학회지 Vol.10 No.1

Chronic granulomatous disease (CGD) is a rare inherited disorder of a defective NADPH oxidase enzyme, resulting in very low or no production of superoxide and subsequent reactive oxygen species. Consequently, patients with CGD are highly susceptible to severe bacterial and fungal infections. CGD is a genetically heterogeneous disease caused by defects in any one of the genes encoding the NADPH oxidase components. CGD generally affects about 3-4 per 1,000,000 individuals; thus, it is surprising that the prevalence of CGD on Jeju Island is 34.3 per 1,000,000 individuals. At present, 20 patients with CGD from 14 unrelated families on Jeju Island have been identified; nine males and 11 females. All patients with CGD tested on Jeju Island had an identical and homozygous mutation (c.7C>T in CYBA, p.Q3X in $p22^{phox}$). Therefore, all patients were autosomal recessive form of CGD. This strongly suggests that the unique and identical mutation in CYBA may be inherited from a common proband. Using mutation-specific primers to detect the mutated allele in CYBA, the frequency of subjects carrying a mutated allele was 1.3% of enrolled subjects from Seogwipo City. Further studies are necessary to elucidate how frequently this mutant allele occurs in the population on Jeju Island. Additionally, it is important to construct a national registry system to understand the pathophysiology of CGD and develop a strategy for long-term therapy.

Xanthomonas campest Pv. glycines 8rd 에서 분리한 Glycinecin A gene promoter 연구

조문제 제주대학교 생명과학연구소 1999 제주생명과학연구 Vol.2 No.-

Xanthomonas campestris pv. glycines 8ra produces bacteriocin, glycinecin, which inhibits the growth of bacteria specifically belong to Xanthomonas spp.. Xanthomonas spp. includes many important plant pathogenic bacteria such as X. c. pv. vesicatoria which causes the bacterial leaf spot in pepper and tomato and X. oryzae pv. oryzae which causes bacterial leaf blight in rice. Glycinecin has many of good properties as a biological control agent. Since glycinecin inhibit the growth of phytopathogenic Xanthomonas sp, it can be used to control disease without any harm to beneficial bacteria for plant mostly belonged to Pseudomonas sp. In addition to that it is safe for user and environment. However, it needs some modification to develop glycinecin as a effective biological control agent. The cosmid clones involved in the production of glycinecin were isolated from the genomic library of X. c. pv. glycines 8ra by Ahn et. al., 1996. The DNA region for bacteriocin production was localized and its DNA sequences for this region had been decided. This region carries 2 putative open reading frames(ORFs) and these 2 ORFs are relevant for the activity of glycinecin in E. coli To study the regulation of glycinecin genes and environmental signals involved in the glycinecin production, promoter analysis using β-galactosidase and β-glucuronidase as a reporter was performed. X. c. pv. glycines 8ra harboring each of ORF1-uidA and ORF2-uidA fusions showed the growth phase-dependent expression of glycinecin genes. The activity of glucuronidase of ORF1-uidA and ORF2-uidA started to increase from the early stationary phase and reach the maximum at the late stationary phase.

의미기반 접근에 의한 이동 트랜잭션 처리

김문자(Moonja Kim),조기환(Gihwan Cho) 한국통신학회 1998 한국통신학회 학술대회 및 강연회 Vol.1998 No.12

In vitro Antioxidant and Cytoprotective Activities of the Extract of Dangyuja (Citrus grandis Osbeck) Leaves

Yunjung Kim,Moonjae Cho,Somi Kim Cho 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.5

The antioxidant activities of the extracts of dangyuja (Citrus grandis Osbeck) leaves were evaluated. The highest phenolic content was obtained from the ethyl acetate fraction (EF) (202.1±0.8 mg GAE/g dried extract) and it exhibited the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity. The cytoprotective effects of EF on oxidative damage induced by tert-butyl hydroperoxide (t-BHP) in a human hepatoma cell line, HepG2 cells, were investigated to understand the intracellular antioxidant mechanisms. Treatment of HepG2 cells with EF prior to oxidative stress was found to inhibit reactive oxygen species (ROS) generation, lipid peroxidation, and DNA damage in a dose-dependent manner. Gas chromatography-mass spectrometry (GC-MS) studies on EF resulted in tentative identification of 19 compounds representing 94.3% of the total content. Taken together, these results demonstrated that EF has excellent antioxidant activities and thus dangyuja leaves have great potential as a source for natural antioxidant which can be applied in food products.

유기인계 및 카바메이트계 농약의 고감수성 아세틸콜린에스테라이즈의 대량생산

김영미,조문제,김소미 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.4

본 실험에서는 acetylcholinesterase(AChE, EC 3.1.1.7)를 이용한 간이 잔류농약 검사법에 필요한, 유기인계 및 카바메이트계 살충제에 대한 감수성이 증가된 AChE(MAChE)를 baculovirus를 이용하여 대량으로 생산하는 시스템을 구축하고 생산된 효소의 특성을 관찰하였다.한라산에서 채취한 초파리에서 AChE의 cDNA를 합성한 수 PCR을 이용하여 AChE의 lipid anchor 부분을 제거하고 site directed mutagenesis에 의해 E107Y, F368L, L408F의 염기서열을 변화시켜 재조합된 MAChE cDNA를 합성하였고 baculovirus vector에 삽입하여 대량생산을 시도하였다. 대량 증식에 필요한 조건으로 감염횟수가 네 번일 때, 그리고 세포수가 2×10^(6) cell/㎖일 때 세포의 증식과 효소의 활성이 극대화됨을 알 수 있었다. His tag을 붙여 Ni-NTA affinity column을 이용하여 MAChE를 정제하였으며, 정제된 효소는 실험조건하에서는 pH(3-10)와 온도(20-50℃)의 변화에 영향을 받지 않았다. 농약 추출액으로 methanol을 사용했을 때가 ethanol을 사용할 때 보다 효과적임을 알 수 있었다. 대표적인 유기인계와 카바메이트계 농약에 대한 저해율을 조사한 결과 재조합된 MAChE는 대만의 집파리 및 변형되지 않은 AChE에 비하여 전반적으로 농약에 대한 감수성이 높은 것으로 나타났다. For the simple rapid bioassay of organophosphorus and carbamate pesticide residues, a mass-production system of acetycholinesterase (AChE, EC 3.1.1.7, MAChE) using baculovirus and insect cell culture was constructed. The cDNA for AChE was synthesized from Drosophila melanogaster in Halla Mountain, the lipid anchor tail was removed by PCR and was used for the site-directed mutagenesis of three amino acid residues (E107Y, F368L, L408F). The mutated cDNA was inserted into the baculovirus vector and expressed in insect cells. Maximum cell growth and enzyme activity were reached when the cells (2×10^(6) cell/㎖) were infected four times at four-day-intervals. His-tag containing MAChE was prufied using Ni-NTA column and used for characterization. The activity was maintained under various pHs (3-10) and temperatures (20-50℃) under experimental conditions. As an extractionn solution for pesticides, methanol is more effective than ethanol. Against major organophosphate and carbamate pesticides, the MAChE showed better sensitivity than AChE and AChE from housefly (Taiwan).

The Prebiotics, a Mushroom (Schizophyllum commune) Derived β-glucan Effect Beneficially the Gut Microbiota on Low-fiber Diet or High-fat Diet Mice Model

Sunghong KIM,Moonjae CHO 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.4

한국 문자다중방송의 개발 현황

이상길(SangGil Lee),조문재(MoonJae Cho),정제창(JeChang Jeong),김기호(KiHo Kim),박창섭(ChangSeob Park),설상훈(SangHoon Sull) 대한전자공학회 1985 대한전자공학회 학술대회 Vol.1985 No.9

Alteration of the glutamate and GABA transporters in the hippocampus of the Niemann-Pick disease, type C mouse using proteomic analysis

Byun, Kyunghee,Kim, Jaewoo,Cho, Sang-Yun,Hutchinson, Brian,Yang, Se-Ran,Kang, Kyung-Sun,Cho, Moonjae,Hwang, Kyukye,Michikawa, Makoto,Jeon, Young-Wook,Paik, Young-Ki,Lee, Bonghee WILEY-VCH 2006 Proteomics Vol. No.

<P>Niemann-Pick disease type C (NPC) is a fatal autosomal recessive cholesterol disorder characterized by severe progressive neurodegeneration. To unveil the mechanism of neurodegeneration, proteomic and morphological approaches were applied to the hippocampus in NPC –/– mouse. Two-DE was utilized to resolve the hippocampal protein expression profiles of 4- and 8-week-old NPC +/+ and –/– mice. Differentially expressed protein spots were identified by MALDI-TOF MS and database searching. At 4 weeks of age, there was no significant difference in protein profiles between NPC +/+ and –/– mice. However, at the age of 8 weeks, NPC +/+ and –/– mice showed marked difference in protein expressions. Among these, glutamate receptor 2 precursor was identified. The immunohistochemical study on neurotransporters showed that glial GABA transporter (GAT-3) increased in both 4- and 8-week-old NPC –/– mouse and glutamic acid decarboxylase (GAD-6) increased in 8-week-old NPC –/– mouse. Glial glutamate transporter, excitatory amino acids carrier-1 (EAAC1), decreased in 8-week-old NPC –/– mouse. In conclusion, our data may provide insight into the understanding of the basic mechanism through perturbation of protein networks and neurotransporter systems in a single gene knockout model of NPC disease.</P>