Expression of Wnt Target Genes in Solid Pseudopapillary Tumor of the Pancreas: A Pilot Study

Kang, Chang Moo,Kim, Hyun Ki,Kim, Hoguen,Choi, Gi Hong,Kim, Kyung Sik,Choi, Jin Sub,Lee, Woo Jung Lippincott Williams Wilkins, Inc. 2009 PANCREAS Vol.38 No.2

OBJECTIVES:: Solid pseudopapillary tumor (SPT) of the pancreas is very rare. This study was performed to analyze the expression of Wnt signal target genes (matrix metalloproteinase-7 [MMP-7], cyclin-D1, and c-myc) and Ki-67 in resected SPTs to determine their clinicopathologic characteristics according to their expression. METHODS:: From January 1995 to December 2005, 23 patients underwent pancreatic resections for SPT of the pancreas. Among 23 formalin-fixed, paraffin-embedded tissues, 12 were evaluated as a pilot study. Immunohistochemistry was performed using various detection and antigen retrieval methods to detect MMP-7, cyclin-D1, c-myc, and Ki-67. The expression of Wnt target genes was correlated with clinicopathologic features of the patients. RESULTS:: Solid pseudopapillary tumors of the pancreas always showed cytoplasmic/nuclear accumulation of &bgr;-catenin, frequent expression of cyclin-D1, and low proliferation index. MMP-7, cyclin-D1, c-myc, and Ki-67 were not correlated with microscopic features suggesting malignant potential (P > 0.05). Tumor size was closely related to microscopic features of malignant potential and apparently has an inverse relationship with the expression of cyclin-D1 and Ki-67 (P < 0.05). CONCLUSIONS:: Low proliferative index and associated MMP-7 expression may cause an unpredictable clinical course in this tumor. Subtle changes in the intracellular environment, not pathologic (morphologic) changes, may elucidate the unpredictable clinical course of this tumor.

배추 시스테인 단백질 분해 효소 억제 유전자 BCPI-1의 발현과 종자 발아 및 유묘 생장과의 연관성

홍준기,이은영,김정률,양경애,최영주,정우식,김호일,윤대진,이상열,조무제,임체오 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Phytocystatins are protein inhibitors of cysteine proteinases of the papain family that have been identified in both monocot and dicot plants. A cDNA encoding a phytocystatin, BCPI-1 (Brassica Cysteine Proteinase Inhibitor-1) has been isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Here, we tested whether BCPI-1 transcription is regulated by hormones, and could be involved in regulating cysteine proteinases during and after germination. BCPI-1 was sensitive to exogenous GA_(3) and ABA, which are important factors controlling seed germination, suggesting that the expression of BCPI-1 is hormonally regulated. We introduced a recombinant plasmid containing the full-length BCPI-1 cDNA under the control of cauliflower mosaic virus 35S promoter into rice embryogenic calli using the particle delivery method, and regenerated a number of transgenic rice plants. Constitutively over-expressed BCPI-1 caused changes in overall plant growth and development, including reduced germination and seedling growth. These data support the role of the BCPI-1 in the regulation of endogenous proteinases during both seed germination and subsequent seedling development. Phylocystatins은 papain계열의 cysteine 단백질 분해 효소 활성을 특이적으로 억제하는 억제자로 다양한 식물 종으로부터 분리되었다. 본고에서는, 배추 화아 cDNA library로부터 분리된 phytocystatin인 BCPI-1(Brassica Cysteine Proteinase Inhibitor-1)의 생체 내 기능에 대해 연구하였다. 먼저, BCPI-1 전사체는 발아 조절에 중요한 영향을 미치는 GA₃와 ABA에 의해 예민하게 증가, 혹은 감소되는 반응을 보임으로써, BCPI-1이 식물 호르몬의 영향을 받으며, 특히 발아나 유묘의 생장 조절에 관련이 있음을 알 수 있었다. Particle bombardment 방법을 통하여 BCPI-1을 벼의 배 형성 세포 내에 도입, 재분화 시켜 형질 전환 벼를 생성하였다. CaMV 35S promoter의 조절에 의해 지속적인 BCPI-1 발현을 보이는 형질 전환 벼의 경우, 발아와 유묘 생장이 현저히 지연되었다. 위의 결과들을 바탕으로, BCPI-1이 식물체 내에서 생성되는 cysteine 계열의 단밸질 분해 효소 활성을 조절하여 종자의 발아와 유묘의 생장에 영향을 미치는 것으로 추측한다.

1997학년도 신입생 실태조사

채영무,홍기배 충주대학교 학생생활연구소 1997 學生生活硏究 Vol.4 No.-

1997학년도 편입생 실태조사

채영무,홍기배 충주대학교 학생생활연구소 1997 學生生活硏究 Vol.4 No.-

족부백선의 치료에서 터비나핀과 이트라코나졸의 이중맹검 비교연구

김기홍,최종수,박의수,김상원,김수찬,안성구,서무규,서순봉 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.2

터비니핀(terbinafine)의 족부백선에 대한 치료효과를 판정하기 위해 6개 병원의 피부과에서 공동연구로 족부백선환자90명에 대하여 치료완료하였으며 2군으로 나누어 터비나핀 1일 250㎎씩 2주간 경구치료와 이트라코나졸 1일 100㎎씩 4주간 치료를 이중맹검으로 실시하여 임상적 및 진균학적인 검사로 치료효과와 약제의 내약성을 평가하였다. 완치율은 터비나핀군은 치료 1주후 6.5%, 2주후 23.9%, 4주후 69.6%, 6주후 89.1%였으며, 이트라코나졸군은 치료 1주후 0%, 2주후 22.7%4주후 59.1%, 6주후 77.3%이었다. 부작용과 내약성은 양군에서 비슷한 성적을 나타내었다. 이상의 결과로 보아 터비나핀 1일 250㎎씩 2주간의 경구투여는 치료율, 안정성 및 부작용에서 이트라코나졸 1일 100㎎씩 4주간 치료효과와 유사하였다. In this randomized double-blind trial on multicenter study, the efficacy of the new antifungal agent, terbinafine was compared with the triazole antifungal agent, itraconazole, in the treatment of patients with various forms of tinea pedis. One hundred and ten patients(55 terbinafine, 55 itraconazole) with tinea pedis were enrolled. Eleven patients were lost to follow-up(5 terbinafine, 8 itraconazole) and 9 reported adverse reaction with premature discontinuation of therapy. And 90 patients were eligible for follow-up until 6 weeks after starting the treatment. Forty six patients received terbinafine 250㎎ daily for two weeks and 44 received intraconazole 100㎎ daily for 4 weeks. They were checked clinical symptoms and mycological improvement with KOH wet mount and culture during the study. Clinical and mycological cure rate of the terbinafine group was 6.5% at 1 week after intialtreatment, 23.9% at 2 weeks, 69.6% at 4 weeks and 89.1% at 6 weeks and that of the itraconazole group was 0% at 1 week after intial treatment, 22.7% at 2 weeks, 59.1% at 4 weeks, and 77.3% at 6 weeks. Adverse reactions and tolerability of both drugs were not different significantly. All these findings suggest that the effects of terbinafine 250㎎ daily for 2 weeks in the treatment of tinea pedeis was similar to that of intraconazole 100㎎ daily for 4 weeks.

한국산 돼지의 屠畜檢査例에 의한 臟器 病燮調査 : Ⅰ. 肺의 病燮 Ⅰ. About Lesions of the Lungs

朴琦緖,朴應鰒,文武洪,宋政根 진주농과대학 1970 진주농과대학 연구논문집 Vol.- No.9

The lungs of 400 cases which had been collected at the slaughter houses in Pusan, Masan and Chungmu cities were examined histopathologically to clear out the patterns of pathological changes of pigs held in Korea. 1. The most frequent and dominated lesions in the lungs were swine enzootic pneumonia (14%) and lung worm pneumonia(12%). 2. The pneumonic lesions caused by lung worms showing the bronchiolar muscle hypertrophy, eosinophilic infiltration, more limited peribronchiolar lymphocytic cuffing compared with that of swine enzootic pneumonia differentiated each other two diseases histologically. 3. The hyperemia, pulmonary ecema, interstitial pneumonia, and bronchitis occurred also frequently in the lungs, but the occurrences of the broncho pneumonia, abscess, gangrenous or granulomatous lesions were rare.

국가예방접종전산등록체계 개발을 위한 민간의료 기관 하부구조 및 환경 조사연구

이무식,김은영,이건세,이석구,홍지영,김건엽,김대경,신의철,이연경,기몽서,나백주,김영택 한국모자보건학회 2003 한국모자보건학회지 Vol.7 No.2

본태성 고혈압이 동반된 당뇨병환자의 혈중 인슐린 농도

양동호,홍세용,성기범,안무영,윤신구,박형국,양광익,황주호,신현길 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

Multiple lines of evidence link elevated blood pressure with diabetes mellitus. Specifically, it has been proposed that resistance to insulin-stimulated glucose uptake and hyperinsulinemia may play a central role in the cause and clinical course of hypertension. In diabetes mellitus, insulin resistance and/or hyperinsulinemia is an common finding and it is interesting whether the hyperinsulinemia may play a signigicant role in hypertension with DM in the same way as in essential hypertension without diabetes mellitus. The object of this study was to compare insulin and c-peptide levels between age, sex, and obesity matched two groups(DM with DM without hypertension). Method The study group consisted of 55 male diabetes mellitus patients, aged between 45-55 (years). Patients with obesity (body mass index · 30 ㎏/m2), renal disease (proteinuria · 300 ㎎/24hr urine), and secondary hypertension were excluded. Insulin and c-peptide were measured in overnight fasting state and after oral administration of glucose(75 gm). In the fasting, venous plasma glucose levels were similar in the hypertensive and control group(132 ±7 ㎎/dl vs 135 ±8 ㎎/dl). In the fasting, venous plasma insulin levels were higher in the hypertensive than in the control group (10.9 ±5.3 μIU/ml vs 5.5 ±3.9 μIU/ml, p = 0.0001). After loading with 75 gm glucose, venous plasma insulin level seems to be higher in the hypertensive patients than in the control patients, but the difference was not signigicant statistically (27.2 ±17.5 μIU/ml vs 19.9 ±18.9 μIU/ml, p = 0.1297). The mean insulin concentration of the essential hypertensive patients with diabetes mellitus was twice that of the normotensive patients with diabetes mellitus. In control group, there was a direct relations between insulin level and c-peptide, in both fasting state (R = 0.617, p = 0.0001) and glucose-loaded state (R = 0.531, p = 0.001). But in hypertensive group, there was no relations between insulin level and c-peptide, in both fasting state (R = 0.257, p = 0.2738) and glucose-loaded state (R = 0.307, p = 0.1885).

유포변경 간헐폭기식 침전지내장형 산화구시스템에 의한 하수의 탈질,탈인

장덕,홍기호,허준무,한상배 대한상하수도학회 2003 상하수도학회지 Vol.17 No.1

Phased isolation intrachannel clarifier ditch system devised in this study is an innovative biological nutrient removal system employing two ditches with intrachannel clarifiers and eliminating external per-anaerobic reactors and recycle of sludge and treated effluent. The performances of the system were evaluated through a long term pilot-scale experiments. The system operated at hydraulic retention times of 10-21hours and solids retention times of 16-36days showed removals of biochemical oxygen demand, total nitrogen, and total phosphorus in the range of 90-99%, 64-93%, and 63-98%, respectively, at the mixed liquor temperature above 10℃. The solids retention time played a relatively important role among the operational parameters of the system. As the solids retention time became longer, the effluent total nitrogen decreased dramatically, whereas the effluent total phosphorus increased. Optimal system operating strategies maximizing phosphorus release and its uptake as well as nitrification and denitrification satisfying both the best nitrogen and phosphorus removals included hydraulic retention times ranged 10-14hours and solids retention times ranged 20-25days. The phase separation in a cycle maximizing the performance of the system was completely accomplished by scheduling of alternating flow and intermittent aeration in a simplified process scheme. Especially, temporal phase separation for phosphorus release without additional anaerobic reactor was successfully accomplished during anaerobic period without any nitrate interference and carbon-limiting. The phased isolation ditch system with intrachannel clarifier developed in this study was, therefore, believed to be a simplified and improved option to solve the problems in the existing biological nutrient removal technologies.

한국산 돼지의 屠畜檢査例에 의한 臟器 病變調査 : Ⅱ.肝의 病變 Ⅱ.About Lesions of the Liver

박응복,文武洪,朴琦緖,宋政根 진주농과대학 1971 진주농과대학 연구논문집 Vol.- No.10

The organs of 400 cases which had been collected at the slaughter houses in Pusan, Masan and Chungmu cities were examined histopathologically to clear out the patterns of pathological changes of pigs kept in Korea. The report about the lesions of the liver succeeding the former report about the lung was summairzed as follows: 1) The most frequent and dominated lesions in the liver were those of hepatic ascariasis(11%) and toxic changes(35%) such as cloudy swelling, hepatic lipidosis, parenchymatous necrosis and liver cirrhosis. 2) The lesions of the liver cirrhosis observed here would be classified as three types; portal cirrhosis subdivided into secondary cirrhosis and Laennec's type. intralobular cirrhosis and biliary cirrhosis.