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Choi, Mira,Choi, A Young,Ahn, Soo-Yeon,Choi, Kwon-Young,Jang, Kyoung-Soon Korean Society for Mass Spectrometry 2018 Mass spectrometry letters Vol.9 No.3
In this study, the chemical composition of bacterial melanin isolated from the Streptomyces glaucescens strain was elucidated by ultra-high-resolution Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry. Ultra-high-resolution mass profiles of the microbial melanin product were acquired using a 15 Tesla FT-ICR mass spectrometer in positive and negative ion modes via electrospray ionization to obtain more complete descriptions of the molecular compositions of melanin-derived organic constituents. A mass resolving power of 500,000 (at m/z 400) was achieved for all spectra while collecting 400 scans per sample with a 4 M transient. The results of this analysis revealed that the melanin pigment isolated from S. glaucescens predominantly exhibits CHON and CHO species, which belong to the proteins class of compounds, with the mean C/O and C/N ratios of 4.3 and 13.1, thus suggesting that the melanin could be eumelanin. This analytical approach could be utilized to investigate the molecular compositions of a variety of natural or synthetic melanins. The compositional features of melanins are important for understanding their formation mechanisms and physico-chemical properties.
( Mira Choi ),( A Young Choi ),( Soo-yeon Ahn ),( Kwon-young Choi ),( Kyoung-soon Jang ) 한국질량분석학회 2018 Mass spectrometry letters Vol.9 No.3
In this study, the chemical composition of bacterial melanin isolated from the Streptomyces glaucescens strain was elucidated by ultra-high-resolution Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry. Ultra-high-resolution mass profiles of the microbial melanin product were acquired using a 15 Tesla FT-ICR mass spectrometer in positive and negative ion modes via electrospray ionization to obtain more complete descriptions of the molecular compositions of melaninderived organic constituents. A mass resolving power of 500,000 (at m/z 400) was achieved for all spectra while collecting 400 scans per sample with a 4 M transient. The results of this analysis revealed that the melanin pigment isolated from S. glaucescens predominantly exhibits CHON and CHO species, which belong to the proteins class of compounds, with the mean C/O and C/N ratios of 4.3 and 13.1, thus suggesting that the melanin could be eumelanin. This analytical approach could be utilized to investigate the molecular compositions of a variety of natural or synthetic melanins. The compositional features of melanins are important for understanding their formation mechanisms and physico-chemical properties.
Choi, Yuri,Park, Mira,Kyoung Shin, Hye,Liu, Yanan,Choi, Ja-Wun,Nirmala, Rajkumar,Park, Soo-Jin,Kim, Hak-Yong Elsevier 2014 Materials letters Vol.123 No.-
Polyacrylonitrile (PAN)-based electrospun nanofibers (NFs) were irradiated using an electron beam. 1% hydrogen peroxide (H2O2) solution was sprayed during Electron Beam Irradiation (EBI) as a further oxidative stabilization process. Fourier transform infrared spectroscopy (FT-IR) and X-ray Diffraction (XRD) of electrospun PAN NFs indicated that the transformation of C=N to C=N groups was accelerated by spraying 1% H2O2 solution. EBI-H2O2 stabilized PAN NFs reduce the onset temperature, extends the exothermic reaction, and improves the stabilization at a significantly lower dose of irradiation (50 kGy) as a result of cyclization and oxidation of PAN molecules caused by 1% H2O2 solution. (c) 2014 Elsevier B.V. All rights reserved.
( Mira Choi ),( Jang Hee Oh ),( Min Kyeong Shin ),( Se Rah Lee ),( Dong Hun Lee ),( Seon Pil Jin ),( Soyun Cho ),( Jin Ho Chung ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2
Background: Recent study has indicated that expression of ABO blood group (ABH) antigens are reduced in the sun exposed skin, suggesting that ABH antigens may be implicated in photoaging process. Objectives: To investigate the potential effects of ABH antigen synthesis-increasing molecules on the extracellular matrix protein production in human skin, which are mixtures of 5 natural plant extracts (Camellia sinensis leaf extract, Polygonum cuspidatum root extract, Ginkgo biloba leaf extract, Cynara scolymus leaf extract, Selaginellatamariscina extract) and 5 monosaccharides (D-Glucose, D-Galactose, L-fucose, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine). Methods: Effects of one-time topical application with composition TYPE I (5 natural plant extracts) and TYPE II (5 monosaccharides) to the human buttock skin (n=6) and 24-week application with TYPE III (all of the ingredients) to the facial skin (n=22 for each) on extracellular matrix (ECM) protein expressions, wrinkle, and elasticity were determined by immunohistochemistry, Skin Viosiometer, and Cutometer. Results: One-time application of TYPE I and II increased perlecan and fibrillin-1 staining, respectively. 24-week application of TYPE III showed no significantimprovement of wrinkle and elasticity. However, it increased procollagen 1メ, tropoelastin, and fibrillin-1staining. Conclusion: These results suggest that these ingredients may help ECM protein production in human skin by long-term application for anti-aging cosmetic products.