규제품질관리를 위한 정책수단의 활용 및 상호관계 분석: 규제비용편익분석과 이해관계자 협의를 중심으로

이민호 ( Lee¸ Minho ) 한국행정학회 2021 韓國行政學報 Vol.55 No.3

과거 규제품질관리의 목적에서 전 세계적으로 규제영향분석의 도입이 활발히 진행되어 왔으며, 그 핵심적 항목으로 규제비용편익분석의 중요성이 강조되었다. 최근에는 정책과정에서의 소통과 참여가 강조되면서 규제협의에 대한 측면도 규제품질관리의 중요한 요소로 주목되고 있다. 본 연구는 규제품질관리제도의 중요한 정책수단으로 비용편익분석과 이해관계자 협의의 활용이 어떻게 이루어지고 있으며, 이들 간에 어떠한 상호관계를 나타내는지 실증적으로 분석하고자 하였다. 예비분석 차원에서 OECD의 iREG 조사결과와 우리나라 규제혁신평가 결과를 활용한 분석 결과는 규제비용편익분석과 규제협의 수준이 상당히 긍정적인 상관관계를 갖고 있는 것으로 확인되었다. 이어진 본 분석에서는 2016-2019년까지 수행된 4,284건의 규제영향분석서를 대상으로 다각적인 분석을 진행하였으며, 전반적으로 이해관계자에 대한 협의가 활발히 진행될수록 규제비용편익분석의 수준이나 충실도가 높아지는 것을 확인할 수 있다. 또한 규제 비용편익분석 수준에 대한 도구변수를 이용한 분석 결과, 반대 방향에서 규제비용편익분석의 충실도가 높아질수록 이해관계자에 대한 협의도 활성화되는 것으로 파악되었다. 이러한 결과는, 정부규제의 품질수준을 높이기 위한 경제적 분석과 정치적 논의의 수행이 선순환된다는 기존의 이론적 논의를 실증적으로 확인하고 있으며, 향후 양자의 균형적인 제도의 발전 및 활용, 개선이 이루어질 필요성을 제기한다. Since regulatory impact analysis was actively adopted worldwide for the purpose of regulatory quality management in the 1990s, the importance of regulatory cost-benefit analysis has been emphasized as a key policy tool. However, recently, as public communication and participation in the policy process have been more emphasized, regulatory consultation is also drawing more attention as an important tool for regulatory quality management. This study empirically investigates how well cost-benefit analysis and regulatory consultation with stakeholders are utilized as policy tools and what kinds of relationships they have in the current regulatory quality management system. For preliminary analysis, OECD iREG survey results and departmental evaluation results for regulatory innovation in Korea are utilized. They reveal a positive relationship between cost-benefit analysis and regulatory consultation. The results of the subsequent main analysis of 4,284 RIA statements in 2016 -2019 also confirm that more active regulatory consultations have a positive effect on the possibility of conducting a more substantial cost-benefit analysis. In addition, according to the results of a regression analysis using the instrument variable for enhanced cost-benefit analysis, it turns out that the positive influence can also be applicable in the reverse direction. In conclusion, these results empirically confirm the existing theoretical discussion that the policy tools of economic analysis and political discussion have synergistic effects for increasing the quality of government regulations, and suggest the need for balanced development and utilization of these economic and political tools in the regulatory management system.

Functional Conservation of RNase III-like Enzymes: Studies on a Vibrio vulnificus Ortholog of Escherichia coli RNase III

Lee, Minho,Ahn, Sangmi,Lim, Boram,Lee, Dong-Ho,Lee, Kangseok Springer-Verlag 2014 Current microbiology Vol.68 No.4

Preventive Pest Management Using Field Borders and Companion Plants for Organic Farming

Minho Lee,Byungmo Lee,Eun Jung Han,Jong-Ho Park,Sang-Beom Lee,Kwang-Lai Park,Jin-Ho Kim 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04

Materials are limited for pest control and chemical pesticides are prohibited in organic farming. Thus, the concept of pest control may not be effective for pest management in organic farm. As an alternative approach, landscape management is potentially effective and useful approach for preventing pathogen and insect pests in organic farm. It is needed to develop best practices utilizing functional biodiversity for pest management through landscape management. For this purpose, we investigated the effect of companion plants on species richness of insect pests and natural enemies in the field border area and in the crop plants (rice and soybean). In rice paddy fields, Lysimachiae foenum-graecum, Saururus chinensis were selected as effective companion plants on the paddy field levee for conserving Arachnidae as natural enemy and density suppression of insect pest such as Lissorphoptrus oryzophilus, Riptortus clavatus. As a result, damage rate of rice plant by insect pests was significantly lower (2.5%) and yield rate of healthy grains was significantly higher (8.7%) than control. In soybean fields, Marigold plants such as Tagetes patula, T. officinalis, increased Arthropod species richness and ratio of natural enemy group, and decreased ratio of insect pest group. As a result, yield (weight of 100 seeds) of healthy soybean was significantly increased (2.5%) than control. The 2nd infection rate from damage by R. clavatus was significantly lower in the treated plot than that in control. Thus, yield rates of healthy soybean in the control and the companion plant-treated plot were 79.0% and 84.3%, respectively, compared to conventional farming using chemical pesticides. Therefore, use of companion plants on field border was an effective practices for preventing pest insects and increasing yield in organic farming.

Dance motion generation by recombination of body parts from motion source

Lee, Minho,Lee, Kyogu,Lee, Mihee,Park, Jaeheung Springer-Verlag 2018 INTELLIGENT SERVICE ROBOTICS Vol.11 No.2

<P>In this paper, we propose an approach to synthesize new dance routines by combining body part motions from a human motion database. The proposed approach aims to provide a movement source to allow robots or animation characters to perform improvised dances to music, and also to inspire choreographers with the provided movements. Based on the observation that some body parts perform more appropriately than other body parts during dance performances, a correlation analysis of music and motion is conducted to identify the expressive body parts. We then combine the body part movement sources to create a new motion, which differs from all sources in the database. The generated performances are evaluated by a user questionnaire assessment, and the results are discussed to understand what is important in generating more appealing dance routines.</P>

Knockdown of vps54 aggravates tamoxifen-induced cytotoxicity in fission yeast

Lee, Sol,Nam, Miyoung,Lee, Ah-Reum,Baek, Seung-Tae,Kim, Min Jung,Kim, Ju Seong,Kong, Andrew Hyunsoo,Lee, Minho,Lee, Sook-Jeong,Kim, Seon-Young,Kim, Dong-Uk,Hoe, Kwang-Lae Korea Genome Organization 2021 Genomics & informatics Vol.19 No.4

Tamoxifen (TAM) is an anticancer drug used to treat estrogen receptor (ER)-positive breast cancer. However, its ER-independent cytotoxic and antifungal activities have prompted debates on its mechanism of action. To achieve a better understanding of the ER-independent antifungal action mechanisms of TAM, we systematically identified TAM-sensitive genes through microarray screening of the heterozygous gene deletion library in fission yeast (Schizosaccharomyces pombe). Secondary confirmation was followed by a spotting assay, finally yielding 13 TAM-sensitive genes under the drug-induced haploinsufficient condition. For these 13 TAM-sensitive genes, we conducted a comparative analysis of their Gene Ontology (GO) 'biological process' terms identified from other genome-wide screenings of the budding yeast deletion library and the MCF7 breast cancer cell line. Several TAM-sensitive genes overlapped between the yeast strains and MCF7 in GO terms including 'cell cycle' (cdc2, rik1, pas1, and leo1), 'signaling' (sck2, oga1, and cki3), and 'vesicle-mediated transport' (SPCC126.08c, vps54, sec72, and tvp15), suggesting their roles in the ER-independent cytotoxic effects of TAM. We recently reported that the cki3 gene with the 'signaling' GO term was related to the ER-independent antifungal action mechanisms of TAM in yeast. In this study, we report that haploinsufficiency of the essential vps54 gene, which encodes the GARP complex subunit, significantly aggravated TAM sensitivity and led to an enlarged vesicle structure in comparison with the SP286 control strain. These results strongly suggest that the vesicle-mediated transport process might be another action mechanism of the ER-independent antifungal or cytotoxic effects of TAM.

Damage of Anoplophora horsfieldii (Hope) on Celtis sinensis in Jeju, Korea

Minho Lee,Sun Keun Lee,Min-Jung Kim,Seunghyun Lee,Seunghwan Lee 한국응용곤충학회 2023 한국응용곤충학회 학술대회논문집 Vol.2023 No.10

Anoplophora horsfieldii (Hope, 1843), 국명 노랑알락하늘소(가칭)는 아열대 지역에 주로 분포하는 종으로 2019 년에 제주도 용연계곡에서 처음 발견되었으며, 2023년에 국내 정착이 보고되었다. 국내에서는 팽나무에서 발생 하는 것으로 알려졌으나, 섭식방식, 산란 선호성 등 생태적 특성에 대해서는 국내외 연구가 전무하다. 따라서 본 연구에서는 노랑알락하늘소의 기초적인 생태정보를 제공하고자 실내 산란 선호성 조사 및 야외조사를 진행 했다. 산란 선호성 실험은 기주식물로 알려진 팽나무, 멀구슬나무와 제주도내 대표 수종인 동백나무, 종가시나무 을 대상으로 했다. 이 중 팽나무에서만 산란이 확인었으며, 상대적으로 굵은 팽나무에서 더 많은 산란이 확인되었 다. 야외조사에서는 최대 발생지인 용연계곡을 기준으로 반경 3 km 내에서 노랑알락하늘소의 탈출공이 확인된 32 그루의 팽나무를 대상으로 피해를 조사하였다. 나무의 굵기는 흉고직경이 20-40 cm인 팽나무에서 탈출공이 많이 확인되었고, 높이는 지면으로 부터 0-100 cm 구간에서 가장 많은 탈출공이 발견되었다.

Occurrence of Bemisia tabaci and its vectored virus in Korea

Minho Lee,Ki-Baik Uhm,Sunyoung Lee,Heeyong Park,Murad Ghanim 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

Nationwide occurrence of Bemisia tabaci Q biotype was identified from 2005 May to 2007 Dec. in total 28 cities/counties of 9 provinces such as Goyang (Kyung-gi), Gangnung (Gang-won), Jincheon (Chung-buk), Buyeo (Chung-nam), Seongju (Kyung-buk), Geoje (Kyung-nam), Bukjeju (Jeju). Host plants of the scale of B. tabaci Q biotype were over 15 crops of tomato, sweet pepper, hot pepper, eggplant, etc. and total 12 species of weeds such as Veronica persica, Ipomoea lacunosa, Conyza sumatrensis, I. hederacea, Xanthium canadense, Humulus japonicus, Boehmeria nivea, Artemisia vulgaris, Paederia scandens, Acalypha austeralis, Brassica juncea, Rumex crispus. For molecular identifying Bemisia tabaci B and Q biotypes, and Trialeurodes vaporariorum, for which it is difficult to distinguish morphologically, sequences of mitochondrial 16S rDNA and CO I (Cytochromoxidase I) gene were analyzed and restriction enzymes were selected for biotypespecific cleaved bands. As the results, Hinf I for 16S rDNA and Vsp I for CO I gene made specific band patterns for the B and Q biotypes in gel electrophoresis. Thus these methods were able to identify those biotypes and species without DNA sequence analysis. Populations of the Q biotype were collected in each regions of Korea from 2005 to 2007, and they were genetically compared using CO I gene sequences. Thus the populations were divided by three different groups which were introduced over 3-4 times before 2007 from different population sources. Geoje and Jeju were suggested as the first regions of introduction. Especially the populations in the first introduced regions were highly homologous with the Q biotype of Japan. In addition, infection pattern of secondary symbionts in populations of the B and Q biotypes in Korea were different from the Israeli populations. Thus it is suggested that Japan is the main source of B. tabaci Q biotype introduced to Korea. In addition, populations of the both of B and Q biotype in Korea were infected by Haemiltonella, which is more effectively related to the transmission of tomato yellow leaf curl begomovirus (TYLCV). Therefore it is needed to monitor continuously if the outbreak of begomovirus vectored by B. tabaci. In this molecular phylogenetic analysis, it was shown that the population of B. tabaci Q biotype in weed plants near greenhouse was introduced to crop plants in greenhouse. Therefore we understand that weed control is important to inhibit recurrence of B. tabaci in greenhouse. Three species of begomovirus, sweet potato leaf curl virus (SPLCV), tobacco leaf curl virus (TLCV), and TYLCV, were reported after introduction of B. tabaci in Korea. Especially Korean government removed all plants in the first TYLCV-occurred greenhouse in 2008. Multiplex PCR diagnosis between TLCV and TYLCV was developed for the more rapid and accurate monitoring. TLCV and TYLCV strains occurred in Korea were highly homologous with strains of Japan. Therefore these results support our suggestion that Japan is the main source of B. tabaci Q biotype introduced to Korea.

Editor’s Highlight: A Genome-wide Screening of Target Genes Against Silver Nanoparticles in Fission Yeast

Lee, Ah-Reum,Lee, Sook-Jeong,Lee, Minho,Nam, Miyoung,Lee, Sol,Choi, Jian,Lee, Hye-Jin,Kim, Dong-Uk,Hoe, Kwang-Lae ACADEMIC PRESS 2018 TOXICOLOGICAL SCIENCES Vol.161 No.1

<P><B>Abstract</B></P><P>To identify target genes against silver nanoparticles (AgNPs), we screened a genome-wide gene deletion library of 4843 fission yeast heterozygous mutants covering 96% of all protein encoding genes. A total of 33 targets were identified by a microarray and subsequent individual confirmation. The target pattern of AgNPs was more similar to those of AgNO<SUB>3</SUB> and H<SUB>2</SUB>O<SUB>2</SUB>, followed by Cd and As. The toxic effect of AgNPs on fission yeast was attributed to the intracellular uptake of AgNPs, followed by the subsequent release of Ag<SUP>+</SUP>, leading to the generation of reactive oxygen species (ROS). Next, we focused on the top 10 sensitive targets for further studies. As described previously, 7 nonessential targets were associated with detoxification of ROS, because their heterozygous mutants showed elevated ROS levels. Three novel essential targets were related to folate metabolism or cellular component organization, resulting in cell cycle arrest and no induction in the transcriptional level of antioxidant enzymes such as Sod1 and Gpx1 when 1 of the 2 copies was deleted. Intriguingly, <I>met</I>9 played a key role in combating AgNP-induced ROS generation via NADPH production and was also conserved in a human cell line.</P>

Hymenobacter swuensis sp. nov., a Gamma-Radiation-Resistant Bacteria Isolated from Mountain Soil

Lee, Jae-Jin,Srinivasan, Sathiyaraj,Lim, Sangyong,Joe, Minho,Lee, Sang Hee,Kwon, Shin Ae,Kwon, Yoon Jung,Lee, Jin,Choi, Jin Ju,Lee, Hye Min,Auh, Young Kyung,Kim, Myung Kyum Springer-Verlag 2014 Current microbiology Vol.68 No.3

Cryptic diversity of the gall-forming aphid Tuberaphis Takahashi (Hemiptera: Aphididae) on Styrax (Styracaceae)

Minho Lee,Mariusz Kanturski,Hsin-Ting Yeh,Sun Keun Lee,Seunghwan Lee 한국응용곤충학회 2023 한국응용곤충학회 학술대회논문집 Vol.2023 No.10

The aphid genus Tuberaphis Takahashi belongs to the tribe Cerataphdini (Hemiptera: Hormaphidinae) and comprises 17 valid species. Generally, its species are distributed in East and South East Asia, where they primarily feed on Styrax spp. (Styracaceae) and utilize species within the Loranthaceae family as their secondary hosts. They form various shapes of galls on Styrax, looking coral and broccoli etc. Currently, the only aphid species reported to exclusively produce gall forms resembling broccoli head-shaped is Tuberaphis takeouchii (Takahashi). For the first time in Korea, an aphid species forming gall structures with a broccoli-head-like appearance has been discovered. We confirmed that the species discovered in Korea is a new species through morphological and molecular comparisons with T. takenouchii (Takahashi). To comprehend the life cycle of this new species, we conducted a year-long study investigating how the gall forms change and examined aphid polymorphism across different seasons. The results of this study additionally include as followings: 1) Species identification, 2) DNA barcoding, 3) population genetic analyses, and 4) morphological comparison using SEM