Collagen으로 유발된 생쥐의 관절염에 대한 당귀사역가오수유생강탕(當歸四逆加吳茱萸生薑湯)의 효과

김민균 ( Min Kyun Kim ),오민석 ( Min Seok Oh ) 한방재활의학과학회 2011 한방재활의학과학회지 Vol.21 No.2

Objectives: This study was carried out to know the effects of Danggwisayeokgaohsuyusaenggang-tang(hereinafter referred to DST) on arthritis induced by collagen on DBA/1 OlaHsd mice. Methods: For this purpose, DST was orally administered to mouse with arthritis induced by collagen Ⅱ. Cytotoxicity, high performance liquid chromatograph(HPLC) analysis, arthritis index, value of immunocyte in draining lymph node and paw joint, cytokine were measured in vivo. Results: 1. The cytotoxicity against human fibroblast cells(hFCs) was not measured in any concentration. 2. In HPLC analysis, There are high peak patterns at 8 minute(min), 12 min, 35 min, 45 min. 3. The arthritis index was decreased significantly. 4. The degree of arthritis induced damage of joint of DST group is slight compared with control group in histopathologic observation(Hematoxylin and eosin stain(H&E), Masson`s trichrome(M-T) staining). 5. In total cell counts of draining lymph node(DLN) and paw joint, the cells in DLN decreased significantly on DST 200 mg/kg and the cells in paw joint decreased significantly on 200 mg/kg and 50 mg/kg. 6. In DLN, CD4+/CD25+, CD3+/CD69+, major histocompatibility complex(MHC) class-II/CD11c+ cells decreased significantly on DST 200 mg/kg and 50 mg/kg, CD3+/CD8+ cells decreased significantly on DST 200 mg/kg, CD4+, CD3+/CD44+ cells decreased. 7. In paw joints, CD4+, CD11b+/Gr-1+ cells decreased significantly on DST 200 mg/kg and 50 mg/kg. 8. In joints, levels of IL-1β, IL-6, TNF-α, cyclo-oxygenase-2(COX-2), NOS-II were decreased on DST 200 mg/kg and DST 50 mg/kg. 9. In analysing of cytokine in CD3/CD28 activated spleen, IL-17 was decreased significantly, IL-4 was increased significantly and INF-γ was decreased on DST 200 mg/kg. 10. In analysing of cytokine in collagen activated spleen, IL-17 were decreased significantly, IL-4 was increased significantly. Conclusions :This results demonstrated that DST suppressed the inflammatory progression of collagen-induced arthritis(CIA) mice and supported further studies are required to survey continuously in looking for the effective substance and mechanism in the future.

자바리, Epinephelus bruneus의 난 발생과 부화에 미치는 수온의 영향

양문호,최영웅,정민민,구학동,오봉세,문태석,이창훈,김경민,한석중,Yang, Moon-Ho,Choi, Young-Ung,Jung, Min-Min,Ku, Hag-Dong,Oh, Bong-Sae,Moon, Tae-Seok,Lee, Chang-Hoon,Kim, Kyong-Min,Han, Seock-Jung 한국발생생물학회 2007 발생과 생식 Vol.11 No.2

This study examined temperature effect in egg development and hatching of longtooth grouper, Epinephelus bruneus. Fertilized embryos was not growth after morula stage at $15^{\circ}C$, at 18, 21, 24 and $27^{\circ}C$, the required time from fertilized embryos to hatching were 70 h. 30 min., 44 h. 10 min., 29 h. 10 min. and 24 h. 30 min., respectively. The hatching rates at $24^{\circ}C$ were higher than the other conditions and the hatching was not occurred at $15^{\circ}C$. These results suggest that the water temperature range of egg development and hatching was $18{\sim}27^{\circ}C$. 이 연구는 실내 사육수조에서 자연산란 후 수정된 난을 대상으로 수온에 따른 난 발생속도와 부화율을 조사하였다. 부화에 이르기까지 각 수온조건에서 소요된 시간은 $15^{\circ}C$에서 상실기 이후 발생이 진행되지 않았고, 18, 21, 24, $27^{\circ}C$에서 각각 70시간 30분, 44시간 10분, 29시간 10분 그리고 24시간 30분이 소요되었다. 부화율은 $15^{\circ}C$에서 0%였고, 18, 21, 24 그리고 $27^{\circ}C$에서 각각 $8.3{\pm}1.2%$, $18.0{\pm}6.2%$, $24.0{\pm}4.0%$ 그리고 $17.0{\pm}7.2%$로 뚜렷한 차이 없이 $24^{\circ}C$에서 다소 높았고 21와 $27^{\circ}C$에서 비슷한 경향이었으며 $18^{\circ}C$에서 가장 낮게 나타났다. 이러한 결과에서 자바리의 난발생 수온범위는 $18{\sim}27^{\circ}C$로 제안된다.

Evaluation of High Power Light Emitting Diodes (HPLEDs) as Potential Attractants for Adult Spodoptera exigua (Hubner) (Lepidoptera Noctuidae)

( Min Seok Oh ),( Chi Hoon Lee ),( Sang Guei Lee ),( Hoi Seon Lee ) 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.3

To evaluate high-power light-emitting diodes (HPLEDs) as potential attractants for Spodoptera exigua adults, attractiveness of specific wavelengths, illuminance intensity, and light-exposure times were investigated and compared to that of fluorescent light. The white light (40 lux treatment) attracted significantly more S. exigua than other attractants. The optimal light-exposure time, based on the highest attraction rate, was 60 min. Evaluation of attraction and repellent rates under optimal conditions showed the white HPLED had the highest attraction rate (91.1%), and the red HPLED had the highest repellence rate (33.3%). Based on relative efficiency values, the white HPLED was about 9.14 times as efficient as the fluorescent light. These data clearly showed that S. exigua showed the greatest attraction to the white HPLED at 40 lux intensity and 60 min light-exposure time.

Evaluation of High Power Light Emitting Diodes (HPLEDs) as Potential Attractants for Adult Spodoptera exigua (Hübner)

Min-Seok Oh,Sang-Guei Lee,Ju-Hyun Jeon,Hoi-Seon Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.10

To response evaluation of high power light emitting diodes (HPLEDs) as potential attractants to the Spodoptera exigua adults, we investigated the attractiveness of specific wavelength, illuminance intensity and light-exposure time, and compared them to the fluorescent. The all light treatments with the 40 lux intensity attracted the significantly highest number of S. exigua. The optimal light-exposure time exhibited the highest attraction rate at the 60 min. When the attraction and repellent rate in the optimal conditions to the S. exigua was surveyed, the white HPLED exhibited the highest attraction rate (91.1%), whereas the red HPLED exhibited the most repellent rate (33.3%). When evatuated of illuminance efficiency with fluorescent as control, white and red HPLED were found to be 9.14 and 10.34 times more efficient than fluorescent. These data clearly show that both the 40 lux intensity and the 60 min light-exposure time by using the white HPLED was the most suitable for attraction of the S. exigua.

농업 부산물 바이오매스 특성에 따른 반탄화 공정 최적화: 1. 질량감소 예측 모델 개발

김석준 ( Seok Jun Kim ),오광철 ( Kwang Cheol Oh ),박선용 ( Sun Young Park ),조라훈 ( La Hoon Cho ),김민준 ( Min Jun Kim ),이충건 ( Chung Geon Lee ),정인선 ( In Seon Jeong ),전영광 ( Yeong Kwang Jeon ),김대현 ( Dae Hyun Kim ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.2

본 연구에서는 국내에서 버려지고 있는 농업부산물의 에너지로서 이용 가능성이 분석되었다. 농업부산물 바이오매스는 화석연료와 동일하게 활용가능하며 지속적으로 생산되는 에너지원이다. 하지만 높은 수분 함량에 따른 보관문제, 화석연료와 비교하여 낮은 발열량등의 단점을 지니고 있어 전처리공정이 요구된다. 따라서 효율적인 활용을 위한 전처리 공정으로 반탄화가 선정되었다. 반탄화공정은 수분제거를 통해 에너지가 증가되어 저장·운송에 이점으로 인하여 최종적인 에너지 가치가 증대된다. 실험은 문헌탐색을 통한 반탄화공정 적용 가능 농업부산물(고추대)과 실험조건(200, 230, 270C, 10, 20, 30, 40 min)이 선정되었으며, 1차원 시뮬레이션을 통한 분석이 이루어졌다. 이때 반탄화 실험을 통하여 발생되는 질량감소량과 발열량 변화량은 높은 상관관계(r²=0.9528)를 가지고 있으며 본 연구에서는 반탄화공정의 질량감소량 예측 모델개발이 수행되었다. 질량감소량 분석을 위하여 열 중량 분석(Thermo Gravimetric Analyzer, TGA)이 수행되었으며 승온속도(5, 7.5, 15, 22.5, 30, 37.5°C min^-1)에 따른 반응속도상수가 도출되었다. 1차원 시뮬레이션 분석을 통하여 바이오매스 내부 온도를 예측하였으며 앞서 도출된 반응속도 상수를 이용하여 질량감소량이 예측되었다. 질량감소량 실험 및 시뮬레이션 결과 평균 r²=0.97로 상관관계를 나타내고 있으며 공정온도별 평균제곱근오차(Root Mean Square Error, RMSE)가 200°C-0.0165, 230°C-0.0191, 270°C-0.0207로 나타났다. 공정온도 증가에 따라 정확도가 감소되었으며, 이는 고온의 실험조건에서 발생되는 비응축가스와 온도변화에 의한 열분해 특성에 기인한 것으로 판단된다. 최종적으로 더 나아가서 질량감소 모델을 통한 발열량 예측을 위한 분석이 이루어져야한다.

청소년들의 최대 골량 향상을 위한 운동 가이드라인

민석기 ( Seok-ki Min ),오태웅 ( Taewoong Oh ),김상현 ( Sang Hyun Kim ),조진경 ( Jinkyung Cho ),정호연 ( Ho-yeon Chung ),박동호 ( Dong-ho Park ),김창선 ( Changsun Kim6 ) 한국운동생리학회(구 한국운동과학회) 2020 운동과학 Vol.29 No.1

PURPOSE: An increase in bone mineral density during adolescence increases resistance to fractures in older age. The Korean Society for Bone and Mineral Research and the Korean Society of Exercise Physiology developed exercise guidelines to increase the peak bone mass (PBM) in adolescents based on evidence through a systematic review of previous research. METHODS: Articles were selected using the systematic method, and the exercise guidelines were established by selecting key questions (KQs) and defining the effects of exercises based on evidence through a literature review for selecting the final exercise method. There were 9 KQs. An online search was conducted on articles published since 2000, and 93 articles were identified. RESULTS: An increase in PBM in adolescence was effective for preventing osteoporosis and fractures in older age. Exercise programs as part of vigorous physical activity (VPA) including resistance and impact exercise at least 5 to 6 months were effective for improving PBM in adolescents. It is recommended that resistance exercise is performed 10 to 12 rep·set^-1 1-2 set·region^-1 and 3 days·week^-1 using the large muscles. For impact exercises such as jumping, it is recommended that the exercise is performed at least 50 jumps·min^-1, 10 min·day^-1, and 2 days·week^-1. CONCLUSIONS: Exercise guidelines were successfully developed, and they recommend at least 5 to 6 months of VPA, which includes both resistance and impact exercises. With the development of exercise guidelines, the incidence of osteoporosis and fractures in the aging society can be reduced in the future, thus contributing to improved public health.

국내외 수용가 맞춤형 수력발전시스템 현대화개발 및 실증

오민환(Min-Hwan Oh),신기석(Ki-Seok Shin),임재황(Jae-Hwang Lim),윤여창(Yeo-Chang Yun),김현아(Hyun-Ah Kim),강석원(Seok-Won Kang),백종명(Jong-Myung Paik),이준용(Jun-Yong Lee) 대한전기학회 2017 대한전기학회 학술대회 논문집 Vol.2017 No.10

폐 허혈-재관류 손상을 유발한 실험견 모델에서 Prostacyclin이 동맥혈 가스 분석 소견, 폐혈관저항과 폐 수분 함량에 미치는 효과

김계민,오용석 대한마취과학회 1999 Korean Journal of Anesthesiology Vol.36 No.2

Background : Many investigations were done about pulmonary protection in lung transplantation which is the most effective treatment of end-stage pulmonary disease. The objective of this study is to verify the effect of prostacyclin on the ischemia-reperfusion injury in terms of the change of arterial blood gas (ABGA), pulmonary vascular resistance (PVR) and pulmonary water content. Methods : In twelve mongrel dogs weighing approximately 20 kg, double-lumen endotracheal tube was intubated and Swan-Ganz catheter was inserted. To obtain control data for water content of left lung right postcaval lobe was resected. Left hilum was snared with umbilical tape after collapse of left lung and tightened to clamp. It was maintained for 90 min. Thereafter, ventilation and perfusion of left lung were restored. To control group (n=6), prostacyclin was not given. To prostacyclin group (n=6), prostacyclin was intravenously administered at 250 ng/kg/min for 20 min, just before ischemia and just after reperfusion. We measured hemodynamic variables and analyzed arterial blood gas before and after ischemia and then at every 1 hour interval. At 4 hours after reperfusion, left lung was resected, and water content was measured with wet-dry method. Results : There was no significant difference between control group and prostacyclin group in ABGA, PVR and water content of lung. However, three subjects of prostacyclin group showed higher PaO2 after reperfusion than that of others. Conclusion : This study shows that protective effect of prostacyclin is not uniform in severely injured canine ischemia-reperfusion model. We conclude that prostacyclin does not have pulmonary protective effect in severe ischemia-reperfusion injury. (Korean J Anesthesiol 1999; 36: 319∼326)

재사용이 가능한 나노복합재료 Fe₃O₄-ACCS-Ag의 제조 및 항균 특성 평가

심재홍,김해원,김진원,서영석,오세강,조민,박정희,오병택,Shim, Jaehong,Kim, Hea-Won,Kim, Jin-Won,Seo, Young-Seok,Oh, Sae-Gang,Cho, Min,Park, Junghee,Oh, Byung-Taek 한국지하수토양환경학회 2015 지하수토양환경 Vol.20 No.3

In this study, Fe₃O₄-ACCS-Ag nanoparticles (NPs) were successfully synthesized using silica extracted from corn cob ash. The synthesized Fe₃O₄-ACCS-Ag NPs were characterized using X-ray diffraction (XRD), scanning electron microscopyenergy dispersive X-ray spectroscopy (SEM-EDX), transmission electron microscopy (TEM) and fourier transform infrared spectroscopy (FTIR). In addition, the potential application of Fe₃O₄-ACCS-Ag NPs as an antibacterial material in water disinfection was investigated using Escherichia coli ATCC 8739 as model bacteria. The antibacterial activity of synthesized composite material showed 99.9% antibacterial effect within 20 min for the tested bacteria. From this experiment, the synthesized Fe₃O₄-ACCS-Ag nanocomposites also hold magnetic properties and could be easily recovered from the water solution for its reuse. The reused nanocomposites presented the decreasing antibacterial efficiencies with the reuse cycle but the composite used three times still killed 90% of bacteria in 20 min.

발바닥 특정 부위 자극이 뇌파에 미치는 효과에 대한 비선형 분석

吳永仙,吳旼錫,宋泰元 대전대학교 한의학연구소 2001 한의학연구소 논문집 Vol.10 No.1

The brain is one of the most complex systems in nature. Brain waves, or the "EEG", are electrical signals that can be recorded from the brain, either directly or throught the scalp. The kind of brain wave recorded depends on the behavior of the animal, and is the visible evidence of the kind of neuronal (brain cell) processing necessary for that behavior. But, EEG had been considered as a virtually infinite-dimensional random signal. However, nonlinear dynamics light on dynamical aspects of the human EEG. The methods of nonlinear dynamics provide excellent tolls for the study of multi-variable, complex system such as EEG. In this study, 20 persons seperated in 2 groups were examined with EGG, one group stimulated on specific area of the sole of the foot with footbed inside the shoes. This experiment resulted in at the group stimulated on specific area of the sole of the fott correlation dimension of P4 and Ol channels increased significantly. Therefore, we obserbed that stimulation on specific area of the body had a constant effections on the specific channels.