유전알고리즘을 이용한 SVC 계통의 안정화에 관한 연구

허동렬,주석민,이종민,정형환 동아대학교 생산기술연구소 2000 生産技術硏究所硏究論文集 Vol.5 No.1

This paper deals with a systematic approach to GA-PI Controller design for static VAR compensator(SVC) using genetic algorithm(GA) to improve system stability. Genetic algorithms(GAs) are search algorithms based on the mechanics of natural selection and natural genetics. To verify the robustness of the proposed method, considered dynamic response of terminal speed deviation and terminal voltage by applying a power fluctuation. Thus, we proved usefulness of GA-PI controller design th improve the stability of single machine-bus with SVC system.

Facile Synthesis and Radioiodine Labeling of Hypericin

Kim, Sang-Wook,Park, Jeong-Hoon,Yang, Seung-Dae,Hur, Min-Goo,Kim, Yu-Seok,Chai, Jong-Seo,Kim, Young-Soon,Yu, Kook-Hyun Korean Chemical Society 2004 Bulletin of the Korean Chemical Society Vol.25 No.8

Hypericin (1,3,4,6,8,13-hexahydroxy-10,11-dimethylphenanthro[1,10,9,8-opqra]perylene-7,14-dione), an antidepressant which is also known to be a potent protein kinase C (PKC) inhibitor was synthesized as a precursor for radioiodine labeling via two step reactions. Malignant glioma cells express higher PKC activity compared to untransformed glial cell. Here we report the synthesis and radioiodine labeling of hypericin as a potential brain tumor imaging radiopharmaceutical. The reference compound, 2-iodohypericin, and its radiolabelled analogues, 2-[$^{123}I$]iodohypericin and 2-[$^{124}I$]iodohypericin have been prepared by the reaction of hypericin with NaI or [$^{123}I$]NaI or [$^{124}I$]NaI. The labeling yield was 60-65% for each analogue and the optimal reaction time was 10 min. The purification and isolation of the labelled products were achieved by a reversed-phase HPLC.

신경교세포 및 RAW 264.7 세포에서 Protein kinase의 활성에 의한 유도성 Nitric oxide synthase의 발현

박상철,노삼길,배소현,박지선,이충재,허강민,석정호,이재흔 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

NO(nitric oxide) plays an important role as neurotransmitter or cytokine, and pathologic factor for some diseases by the large amount production with iNOS(inducible NO synthase) expression in macrophages or glial cells. The expression of iNOS is regulated by various cytokines, protein kinases and transcription factors. In this experiment, to investigate the roles of progein kinase and NF-kB for iNOS expression, the effects of PMA(phorbol 12-myristate 13-acetate), cAMP, and various protein kinase inhibitors on LPS(lipopolysaccharide)-induced iNOS mRNAN expression and nuclear NF-kB binding complex were examined in C6 glial cells and RAW 264.7 cells. In C6 glial cells, iNOS mRNA expression by LPS was induced from 1 hour and peak at 3 hour after treatment. In RAW 264.7 cells, the mRNA was observed from 3 hour and peak at 6 hour. PMA enhanced markedly LPS-induced iNOS mRNA expression and NF-kB binding complex in C6 glial cells, but did not much influence on LPS-induced iNOS mRNA expression in RAW 264.7 cells, in spite of increased LPS-induced NF-kB binding complex at 30 min. cAMP(dibutyryl cAMP) did not much influence on LPS-induced iNOS mRNA expression, by increased LPS-induced NF-kB binding complex in C6 glial cells. However, in RAW 264.7 cells, cAMP increased slightly LPS-induced iNOS mRNA expression without change of NF-kB binding complex. Staurosporine did not influence on LPS-induced iNOS mRNA expression and NF-kB binding complex in C6 glial cells, but in RAW 264.7 cells, decreased LPS-induced iNOS mRNA expression and NF-kB binding complex. Ro-31-8220 did not much influence on LPS-induced iNOS mRNA expression and NF-kB binding complex in C6 glial cells, but in RAW 264.7 cells, decreased significantly LPS-induced iNOS mRNA expression in spite of increased LPS-induced NF-kB binding complex for 3hours. G 6976 did not much influence on LPS-induced iNOS mRNA expression with decreased NF-kB binding complex in C6 glial cells, but in RAW 264.7 cells, decreased iNOS mRNA expression without influence on LPS-induced NF-kB binding complex. Genistein did not influence on LPS-induced iNOS mRNA expression and NF-kB binding complex in C6 glial cells, but in RAW 264.7 cells, decreased LPS-induced iNOS mRNA expression inspite of increased NF-kB binding complex. These results suggest that LPS-induced regulation of iNOS expression or NF-kB activity in C6 glial cells, might be different from RAW 264.7 cells through various protein kinases or other factors.

Consistent and Specific Suppression of Mucin Release from Cultured Hamster Tracheal Surface Epithelial Cells by Poly-L-Lysine

Lee, Choong-Jae,Lee, Jae-Heun,Seok, Jeong-Ho,Hur, Gang-Min,Park, Ji-Sun,Bae, So-Hyun,Jang, Hyeon-Seok,Park, Sang-Cheol The Korean Society of Pharmacology 2003 The Korean Journal of Physiology & Pharmacology Vol.7 No.3

Poly-L-lysine (PLL) was reported to suppress mucin release from airway goblet cells during 30 min treatment period. In this study, we investigated whether PLL consistently suppresses mucin release from cultured airway goblet cells during 24 h after 30 min treatment and also specifically suppresses the release of mucin without any effects on the other releasable glycoproteins. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with $^3H$-glucosamine for 24 h and chased for 30 min in the presence of varying concentrations of PLL to assess the effects on $^3H$-mucin release and on the total elution profile of the treated culture medium. The total mucin content during 24 h after 30 min treatment of PLL was assesed to investigate the consistency of effects. PLL did not affect the release of the other releasable glycoproteins whose molecular weights were less than mucin, and decreased the total mucin content during 24 h after 30 min treatment. We conclude that PLL can specifically suppress mucin release from cultured airway goblet cells and the suppression on mucin release is consistent. This finding suggests that PLL might be used as a specific airway mucin-regulating agent by directly acting on airway mucin-secreting cells.

Consistent and Specific Suppression of Mucin Release from Cultured Hamster Tracheal Surface Epithelial Cells by Poly-L-Lysine

Choong Jae Lee,Jae Heun Lee,Jeong Ho Seok,Gang Min Hur,Ji Sun Park,Sohyun Bae,Hyeon Seok Jang,Sang Cheol Park 대한생리학회-대한약리학회 2003 The Korean Journal of Physiology & Pharmacology Vol.5 No.1

Poly-L-lysine (PLL) was reported to suppress mucin release from airway goblet cells during 30 min treatment period. In this study, we investigated whether PLL consistently suppresses mucin release from cultured airway goblet cells during 24 h after 30 min treatment and also specifically suppresses the release of mucin without any effects on the other releasable glycoproteins. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with <SUP>3</SUP>H-glucosamine for 24 h and chased for 30 min in the presence of varying concentrations of PLL to assess the effects on <SUP>3</SUP>H-mucin release and on the total elution profile of the treated culture medium. The total mucin content during 24 h after 30 min treatment of PLL was assesed to investigate the consistency of effects. PLL did not affect the release of the other releasable glycoproteins whose molecular weights were less than mucin, and decreased the total mucin content during 24 h after 30 min treatment. We conclude that PLL can specifically suppress mucin release from cultured airway goblet cells and the suppression on mucin release is consistent. This finding suggests that PLL might be used as a specific airway mucin-regulating agent by directly acting on airway mucin-secreting cells.

P011 Antioxidant functions of kinetin on damage keratinocytes by hydrogen peroxide

( Min Seok Hur ),( Hye In Cheon ),( Byung Gon Choi ),( Song Hee Han ),( Min Jung Kim ),( Hae Jeong Youn ),( Soo Young Kim ),( Nam Kyung Roh ),( Yang Won Lee ),( Yong Beom Choe ),( Kyu Joong Ahn ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.2

<div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div> Background: Hydrogen peroxide (H2O2) is a species of reactive oxygen, affects to fate of cells in growth, senescence, and extinction. H2O2 is unstable property because of free radicals. As unstable property, H2O2 reacts strong with other molecules and damage to cells and DNA. Kinetin, plant cytokinins, regulates plant growth. It has been reported that kinetin has antioxidant effects, synthesis promotion of DNA, and senescence delay on dermal fibroblast in cellular function. But there is no study reported in effects of kinetin on keratinocytes. Objectives: This study aimed to verify functions of kinetin as a raw material for cosmetics products in skin through the cell efficacy test. Methods: To verify the efficacy of kinetin on HaCaT human keratinocyte cells, the cell viability essay, and reactive oxygen species (ROS) assay were performed. As kinetin with concentrations of 200 μM and above were toxic in HaCaT cells, this study used kinetin with concentrations of less than 200 μM. Results: Cell viability assay showed that kinetin efficiently rescued HaCaT cells from H2O2-induced oxidative stress damages in a concentration-dependent manner. Furthermore, flow cytometry analysis revealed that kinetin decreased the intracellular ROS levels in H2O2-treated HaCaT cells. Conclusion: These data indicated that kinetin can be used as a raw effective natual material for for functional cosmetic products which can reduce facial wrinkles, came from ROS, and improve the condition and function of skin.

[P239] A case of recurrent acute paronychia

( Min Seok Hur ),( Joo Ran Hong ),( Hye In Cheon ),( Song Hee Han ),( Byung Gon Choi ),( Hae Jeong Youn ),( Min Jung Kim ),( Yang Won Lee ),( Yong Beom Choe ),( Kyu Joong Ahn ) 대한피부과학회 2017 대한피부과학회 학술발표대회집 Vol.69 No.1

While acute paronychia is usually caused by bacterial infection, chronic paronychia is a multifactorial inflammatory disease of the nail apparatus due to irritants and allergens. Recurrent acute paronychia may evolve into chronic paronychia. A 23-year-old female was referred to our dermatologic clinic with an erythematous and edematous proximal nail fold of right forth finger. She had been treated with nail extraction and antifungal agent in the diagnosis of acute paronychia due to bacterial and fungal infection. The lesion seemed to be improved but aggravated with abscess formation. We drained pus and carried out wound culture and KOH smear, but we could not get pathogenic findings. We tried cefadroxil and nonsteroidal anti-inflammatory drugs with nail extraction. Despite of repeated treatments, her problem didn’t seem to be improved. For management of recurrent acute paronychia, we decided to add anti-pseudomonal agent to previous treatment. The lesion has kept improved state without relapse. We report an interesting case of recurrent acute paronychia refractory to treatment with antibiotics, antifungal agents and surgical management.

[P056] A case of acquired palmoplantar keratoderma treated with keratolytics and retinoids

( Min Seok Hur ),( Joo Ran Hong ),( Hye In Cheon ),( Song Hee Han ),( Byung Gon Choi ),( Hae Jeong Youn ),( Min Jung Kim ),( Yang Won Lee ),( Yong Beom Choe ),( Kyu Joong Ahn ) 대한피부과학회 2017 대한피부과학회 학술발표대회집 Vol.69 No.1

Palmoplantar keratodermas (PPKs) are disorders with variable causes that are characterized by abnormal hyperkeratosis of the palms and soles. They have been classified as either hereditary or acquired PPKs. The clinical characteristics of acquired PPKs are non-hereditary, non-frictional skin thickening involving acral areas. A 33-year-old woman presented with nonsymptomatic hyperkeratotic patches on palms and soles. She had neither medical history nor family history of keratoderma. We had applied topical keratolytic agent (salicylic acid) for four months, followed by alitretinoin. After four months of the treatment with alitretinoin, she showed clinical improvement. Herein we report a patient with PPK with unknown etiology that led to the diagnosis of acquired PPK. This report would be helpful to suggest successful treatment of acquired PPKs with keratolytics and retinoids.

[P220] A case of multiple basal cell carcinoma in xeroderma pigmentosum treated with CO₂ laser and ingenol mebutate gel

( Min Seok Hur ),( Joo Ran Hong ),( Hye In Cheon ),( Song Hee Han ),( Byung Gon Choi ),( Hae Jeong Youn ),( Min Jung Kim ),( Yang Won Lee ),( Yong Beom Choe ),( Kyu Joong Ahn ) 대한피부과학회 2017 대한피부과학회 학술발표대회집 Vol.69 No.1

Xeroderma pigmentosum (Xp) is a rare autosomal recessive disorder of DNA repair, with a prevalence of 1 in 1 million individuals. It is characterized by extreme sensitivity to sunlight, resulting in sunburn, pigment changes in the skin, a high incidence of skin cancer, and developmental disorders. A 12-year-old girl presented with multiple scaly patches of various sizes and nodules on her face. Histological diagnosis revealed basal cell carcinoma. In addition to the malignancy, she had a pigmentaryabnormality, hypersensitivity to sunlight, neurological hearing loss, intellectual deficiency, and developmental delay. In accordance with the symptoms and findings, she was clinically diagnosed as having Xp. We used carbon dioxide laser to treat the malignant lesions and treated the other small scaly papules with 0.015% ingenol mebutate gel to prevent cancer related to Xp. Herein we report a case of early onset skin cancer occurring in a patient with severe form of Xp.

P012 Novel assessment method of skin hydration using silica gel

( Min Seok Hur ),( Hye In Cheon ),( Byung Gon Choi ),( Song Hee Han ),( Min Jung Kim ),( Hae Jeong Youn ),( Soo Young Kim ),( Nam Kyung Roh ),( Yang Won Lee ),( Yong Beom Choe ),( Kyu Joong Ahn ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.2

<div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div> Background: Skin hydration is referred as the intra- and intercellular water content in the epidermis and the dermis. There are diverse in vivo assessment methods of the water content of the skin, however, in vitro assessment method of cellular water content has not been largely investigated and developed yet. Objectives: We developed a novel in vitro simple assessment method of cellular water content in keratinocytes using coloring silica gel. Methods: We raised question about three aspects; the first is that whether the level of color change of silica gel can be correlated with the level of water content, the second is that whether the changed level of color can be quantifiable, the third is that whether the cellular water of keratinocytes can be absorbed into silica gel. We simply confirmed that its own ‘Red’ color of silica gel was changed to yellow in a H2O concentration dependent manner. Results: The intensity level of red color area of silica gel was significantly decreased as the number of keratinocyte increased. Also the level of its red color was different between undifferentiated keratinocyte and differentiated keratinocytes. Conclusion: These data indicate that the cellular water of keratinocyte can be absorbed into silica gel, and the level of color change of it is statistically correlated with the level of water content of keratinocytes.