초기 자궁내막암 환자에서의 복강경유도하 질식 자궁절제술과 복식 자궁절제술에 대한 비교 연구

김민규 ( Min Kyu Kim ),최동석 ( Dong Seok Choi ),김우영 ( Woo Young Kim ),최철훈 ( Chel Hun Choi ),김태중 ( Tae Joong Kim ),이정원 ( Jeong Won Lee ),김병기 ( Byoung Gie Kim ),이제호 ( Je Ho Lee ),배덕수 ( Duk Soo Bae ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.12

목적: 초기 자궁내막암의 수술적치료 시 복강경유도하 질식 자궁절제술 및 골반림프절절제술군과 복식수술군의 수술결과 및 재발율에 대해 비교하고자하였다. 연구 방법: 2003년 3월부터 2005년 5월까지 초기 자궁내막암 환자를 인구학적 특성이 같은 63명을 선택하여 복강경유도하 질식 자궁절제술 및 골반림프절절제술군 26명, 복식수술군 37명으로 나누어 수술 전후 혈색소 차이, 평균수술시간, 취득한 골반림프절갯수, 평균재원일수, 추가치료율, 재발률을 비교하였다. 결과: 복강경유도하 질식 자궁절제술 및 골반림프절절제술을 시행한 군과 복식수술을 시행한 군과의 비교에서 수술 전후 혈색소 차이, 평균수술시간, 취득한 골반림프절 갯수, 평균재원일수, 재발률에 통계적으로 유의한 차이가 없었다. 결론: 초기 자궁내막암의 수술 시 복강경유도하 질식자궁절제술 및 골반림프절절제술 방법이 복식방법에 비해 수술결과 및 재발률에 통계적으로 유의한 차이가 없으며 그 대체 수술법으로 많은 활용이 기대되었다. Objective: To compare laparoscopic surgery with conventional abdominal surgery in patients with early stage endometrial cancer. Methods: A retrospective review of 63 patients with early stage endometrial cancer managed between March 2003 and May 2005. Two groups were defined whether they had been treated by laparoscopy (case group: n=26) or by laparotomy (control group: n=37). We compared age, body mass index (BMI), hemoglobin change, operation time, number of pelvic lymph nodes, hospital stay, case with adjuvant treatment and recurrence between two groups. Results: There was no statistical difference in characteristics (age, BMI, nulliparity, previous abdominal surgery, FIGO stage, histologic grade). between case and control group. In addition, there was no statistical difference in operation data and outcomes between two groups. Hemoglobin changes were 1.1 g/dL (case group) vs 1.7 g/dL (control group) (p=0.072). Operation time was 131 min vs. 115 min. The numbers of lymph nodes obtained were 8.7 vs 7.7 (right) 9.2 vs. 7.6 (left). Hospital stays were 8.4 vs. 9.2 days. Adjuvant treatment cases were 7 vs. 15. Recurrent case was one in each group. Two patients initially evaluated by laparoscopy were converted to laparotomy due to bleeding and adhesion. Conclusion: Laparoscopic surgery for treatment of early endometrial cancer is a safe and effective alternative to laparotomy . However, long-term survival and risk of recurrence have yet to be determined.

Determining the Optimal Cooking Time for Cooking Loss, Shear Force, and Off-Odor Reduction of Pork Large Intestines

Sung-Su Kim,Yee Eun Lee,ho Hyun Kim,Joong-Seok Min,Dong Gyun Yim,Cheorun Jo 한국축산식품학회 2022 한국축산식품학회지 Vol.42 No.1

The objective of this study was to determine the optimal cooking time by considering the cooking loss, shear force, and off-odor reduction of pork large intestines. Commercial pork large intestines were purchased, quartered perpendicularly, and cooked in boiling water for 40, 120, 180, and 240 min. Cooking loss of the samples increased after 240 min of cooking (10.92, p<0.05) while shear force value was lower at 240 min (4.45) compared to that at other cooking times (p<0.001). The amount of major volatile organic compounds showed a decreasing trend with increasing cooking time. In particular, the amount of methyl pentanoate (17,528.71) and methyl isobutyrate (812.51), compounds with a relatively low odor threshold, decreased significantly after 120 min of cooking and no change was observed thereafter (p<0.05). In addition, the amount of 2-pentanol (3,785.65) and 1-propanol (622.26), possibly produced by lipid oxidation, significantly decreased at the same cooking time (p<0.001). In the principal component analysis, only the 40 min cooking time was significantly different from other cooking time by high amounts of 1-propanol, 2-pentanol, and methyl isobutyrate. In conclusion, in the present study, the optimal cooking time for pork large intestines was 120 min in terms of off-odor reduction, cooking loss, and shear force.

신규 3작용성 메타크릴레이트의 치과용 광중합형 콤포짓트 레진용 단량체로서의 응용 연구

김중곤,정찬문,김민성,김광만,김경남 大韓齒科器材學會 2001 대한치과재료학회지 Vol.28 No.4

Tris[4-(2-hydroxy-3-methacryloxypropoxy)phenyl]methane(THMPM) was prepared easily from triphenylolmethane triglycidyl ether in good yield. As compared with composite resin formulated with difunctional bis-GMA, the physical properties of a newly developed composite resin formulated with trifunctional THMPM was evaluated. Cytotoxicity and acute toxicity of composite resin based on trifunctional THMPM evaluated according to ISO standard method. The depth of cure of the composite resin based THMPM was higher than that of the bis-GMA composite. There were no significant differences in flexural strength and water sorption between the composites resin based on THMPM and bis-GMA. The water-solubility value for the light-activated composite resins formulated with THMPM was much lower than that for a control bis-GMA composite. According to cytotoxicity evaluation, the composite resin based on THMPM showed very mild cytotoxicity with the response index of between 0/0 and 1/0. No acute toxicity of composite resin formulated with trifunctional THMPM was also observed. It is believed that THMPM is promising candidate for application as a photocurable dental composite resin.

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

Kim's Femoral Stem의 임상적 고찰

김영민(Young Min Kim),김희중(Hee Joong Kim),진종수(Jong Soo Jin),문영완(Young Wan Moon) 대한정형외과학회 1991 대한정형외과학회지 Vol.26 No.5

Slide Session : OS-111 ; Sleep : The Effects of Dexmedetomidine for Sleep in Critically Ill Patients: Pilot Study

( Se Joong Kim ),( Jinsoo Min ),( Jisoo Park ),( Yeon Joo Lee ),( Jong Sun Park ),( Ho Il Yoon ),( Jae Ho Lee ),( Choon Taek Lee ),( Young Jae Cho ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Many critically ill patients in the intensive care unit (ICU) experience sleep disruption and delirium. For the sedation of these patients, dexmedetomidine is one of commonly recommended sedatives because of its pharmacokinetic merit. This pilot study was undertaken to identify the effects of dexmedetomidine for sleep and delirium in critically ill patients. Methods: This prospective study was conducted in medical ICU of a tertiary referral hospital. Dexmedetomidine was administered with the maintenance dose of 0.4-0.7μg/ kg/hr and adjusted by Richmond Agitation Sedation Scale score of 0 to -2. Portable polysomnography was performed in the ICU over 24 hour to assess the quantity and quality of sleep. The confusion assessment method for ICU was used for detection of delirium. Results: Total 9 patients were enrolled. Median age was 77.0 (Range: 61-90) and 3 patients experienced delirium. Median total sleep time was 283 (IQR: 56-739) min. The majority of sleep was stage 1 (median 208 [IQR: 56-356] min) and 2 (median 75 [IQR: 7-396] min) with absent REM and stage 3 sleep. The dose of dexmedetomidine was not associated with total sleep time, stage 1 and stage 2 sleep (all P>0.05). However, the patients with delirium was administered higher dose of dexmedetomidine than ones without delirium (0.67μg/kg/hr vs 0.33μg/kg/hr, P=0.006). Conclusions: Although proper sedation was met with dexmedetomidine, the quantity and quality of sleep in critically ill patients were poor. Further study is required for the promotion of good sleep and the prevention of delirium in ICU patients.

Free Paper Presentation : OS-111 ; The Effects of Dexmedetomidine for Sleep in Critically Ill Patients: Pilot Study

( Se Joong Kim ),( Jin Soo Min ),( Ji Soo Park ),( Yeon Joo Lee ),( Jong Sun Park ),( Ho Il Yoon ),( Jae Ho Lee ),( Choon Taek Lee ),( Young Jae Cho ) 대한결핵 및 호흡기학회 2014 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.118 No.-

Background: Many critically ill patients in the intensive care unit (ICU) experience sleep disruption and delirium. For the sedation of these patients, dexmedetomidine is one of commonly recommended sedatives because of its pharmacokinetic merit. This pilot study was undertaken to identify the effects of dexmedetomidine for sleep and delirium in critically ill patients. Methods: This prospective study was conducted in medical ICU of a tertiary referral hospital. Dexmedetomidine was administered with the maintenance dose of 0.4-0.7μg/kg/hr and adjusted by Richmond Agitation Sedation Scale score of 0 to -2. Portable polysomnography was performed in the ICU over 24 hour to assess the quantity and quality of sleep. The confusion assessment method for ICU was used for detection of delirium. Results: Total 9 patients were enrolled. Median age was 77.0 (Range: 61-90) and 3 patients experienced delirium. Median total sleep time was 283 (IQR: 56-739) min. The majority of sleep was stage 1 (median 208 [IQR: 56-356] min) and 2 (median 75 [IQR: 7-396] min) with absent REM and stage 3 sleep. The dose of dexmedetomidine was not associated with total sleep time, stage 1 and stage 2 sleep (all P>0.05). However, the patients with delirium was administered higher dose of dexmedetomidine than ones without delirium (0.67μg/kg/hr vs 0.33μg/kg/hr, P=0.006). Conclusions: Although proper sedation was met with dexmedetomidine, the quantity and quality of sleep in critically ill patients were poor. Further study is required for the promotion of good sleep and the prevention of delirium in ICU patients.

원인 불명의 실신 환자의 진단에 있어서 Head - up tilt Test 의 유용성과 혈관미주신경성 실신 환자의 임상적 특징

윤호중(Ho Joong Youn),정욱성(Wook Sung Chung),백상홍(Sang Hong Baek),김철민(Chul Min Kim),박인수(In Soo Park),김재형(Jae Hyung Kim),최규보(Kyu Bo Choi),홍순조(Soon Jo Hong) 대한내과학회 1994 대한내과학회지 Vol.47 No.2

N/A Background: Syncope, defined as a transient loss of consciousness, is a frequently encountered symptom, but despite thorough clinical and invasive diagnostic investigation, the cause of syncope remains unexplained. In recent years, head-up tilt test has been of increasing interest as a diagnostic aids in patients with unexplained syncope. We studied to define the usefulness of various diagnostic test in the evaluation of patients with recurrent syncope, to assess the usefulness of head-up tilt test in the working of syncope of unknown origin and to evaluate the clinical characteristics of subgroups of patients with syncope induced by head-up tilt test. Methods: Between June, 1991 and November, 1992 at St. Mary`s hospital, 21 patients with history of syncope and 24 control subjects without history of syncope underwent 60° head-up tild test for 60 min. Results: 1) The definite cause for recurrent syncope were diagnosed in 10 of patients (47,6%) after clinical and invasive tests. 2) During head-up tilt test, vasovagal responses were provoked in 5 of 21 patients (23.8%) with recurrent syncope and 1 of 24 patients (4.2%) without syncope (p<0,05). 3) The head-up tilt test induced symptomatic bradycardia or hypotension in 3 of 10 patients (30%.) with negative electrophysiologic results. 4) During tilt induced vasovagal response, a) mean heart rate decreased to 42±24 beat/min from supine control of 70±12 beat/min (p<0. 05), b) mean systolic blood pressure decreased to 95±12 mmHg from supine control of 120±S mmHg (p<0,05), c) mean diastolic blood pressure decreased to 60±21mmHg from supine control of 72±13 mmHg (p<0.05), d) the time interval to onset of vasovagal response was 22±12 min. Conclusions: 1) In a large proportion of patients with recurrent syncope, the diagnosis remains unexplained despite a neurologic and cardiologic investigation including an electrophysiologic study. 2) Vasovagal responses are frequent cause in patients with recurrent unexplained syncope. 3) The head-up tilt test may be a simple, safe and highly yielding provocative test in the investigation of syncope of unknown origin.

Expression and Characterization of a Novel 2-Deoxyribose-5-phosphate Aldolase from Haemophilus influenzae Rd KW20

Woo, Mi-Hee,Kim, Min Soo,Chung, Namhyun,Kim, Joong-Su The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.5

A codon-optimized 2-deoxyribose-5-phosphate aldolase (DERA) gene from Haemophilus influenzae Rd KW20 was synthesized and expressed in Escherichia coli, and the biochemical properties of its product were investigated. DERA was purified using affinity chromatography and characterized using 2-deoxyribose-5-phosphate as the substrate. Specific activity of the recombinant DERA was $34.1Umg^{-1}$. The pH and temperature optima were 7.5 and $40^{\circ}C$, respectively. Additionally, the recombinant enzyme retained stability up to temperatures below $50^{\circ}C$. Maximal enzyme activity was observed in presence of 300 mM of acetaldehyde. The apparent $K_m$ and $V_{max}$ of purified enzyme towards 2-deoxyribose-5-phosphate were 0.14 mM and $70.42{\mu}mol\;min^{-1}mg^{-1}$ and towards 2-deoxy-D-ribose were 24.77 mM and $1.94{\mu}mol\;min^{-1}mg^{-1}$, respectively. For synthesis of statin intermediates, the bioconversion process for production of (3R, 5S)-6-chloro-2,4,6-trideoxy-erythro-hexose from chloroacetaldehyde and acetaldehyde using the recombinant DERA was studied and this process took 3 h for maximal conversion. This recombinant DERA could be potentially applied in the production of (3R, 5S)-6-chloro-2,4,6-trideoxy-erythro-hexose.

H9c2 심근 세포주에서 외인성 nitric oxide가 허혈에 의한 세포 독성에 미치는 영향

정성구,장현용,김명천,고영관,정주호,배영미,박원서,김대중,유영민,김성수,임성빈 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Background: Nitric oxide(NO) is known to have protective effects on an ischemic heart and to exert triggering effects on ischemic preconditioning. However, the effects of NO during the ischemic period have not been investigated. To investigate the role of exogenous nitric oxide in a model of ischemic heart cell death, we studied the effects of ischemic preconditioning and ischemia in a normal and an ischemic buffer. Methods: Rat cardiac myoblast cells(H9c2) were cultured in a normal and an ischemic buffered medium. For the ischemic culture of heart cells, the cells were cultured in a dessicator with GasPak for 5 hrs. In ischemic preconditioning, the cells were pretreated with ischemic buffer for 5 min and then perfused with normal medium for 30 min. For the measurement of the cytotoxicity, a MTT(3-4-Sdimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay was performed. A DAPI(4',6-diamidino-2-phenylindole dihydrochloride) staining procedure and a flow cytometry analysis were performed to confirm apoptotic cell death by ischemia. Results: Cell viability, as determined by using a MTT assay, showed that the preconditioned group treated with NO showed more cell death than with the not-preconditioned groups in both normal and ischemic buffers. But, In normal medium and not-preconditioned groups, NO showed protective effect according to the concentrations(100,1000μM) . No treatment with NO produced the different results. In normal medium, the protective effect of ischemic preconditioning was demonstrated, but no protective effect of ischemic preconditioning could be seen in the case of the ischemic buffer. The DAPI staining and flow cytometry analysis of heart cells showed characteristic apoptotic features. Conclusion: NO added in the ischemic phase had deterious effects on heart cells. Ischemic preconditioning was more harmful than ischemia alone. The toxicity of the cells was characteristic apoptosis.