Evolutionary dynamics of transposable elements during silkworm domestication

Min‑Jin Han,Hong‑En Xu,Xiao‑Min Xiong,Hua‑Hao Zhang 한국유전학회 2018 Genes & Genomics Vol.40 No.10

Although there are some documented examples on population dynamics of transposable elements (TEs) in model organisms, the evolutionary dynamics of TEs in domesticated species has not been systematically investigated. The objective of this study is to understand population dynamics of TEs during silkworm domestication. In this work, using transposondisplay we examined the polymorphism of seven TE families [they represent about 59% of silkworm (Bombyx mori) total TE content] in four domesticated silkworm populations and one wild silkworm population. Maximum likelihood (ML) was used to estimate selection pressure. Population differentiation and structure were performed by using AMOVA analysis and program DISTRUCT, respectively. The results of transposon-display showed that significant differentiation occurred between the domesticated silkworm and wild silkworm. These TEs have experienced expansions and fixation in the domesticated silkworm but not in wild silkworm. Furthermore, the ML results indicated that purifying selection of TEs in the domesticated silkworm were significantly weaker than that in the wild silkworm. Interestingly, an adaptation insertion induced by BmMITE-2 was found, and this insertion can reduce the polymorphism of the flanking regions of its neighboring COQ7 gene. Our results suggested that TEs expanded and were fixed in the domesticated silkworm might result from demographic effects and artificial selection during domestication. We concluded that the data presented in this study have general implication in animal and crop improvements as well as in domestication of new species.

FSCB phosphorylation in mouse spermatozoa capacitation

( Shun Li Liu ),( Bing Ni ),( Xiang Wei Wang ),( Wen Qian Huo ),( Jun Zhang ),( Zhi Qiang Tian ),( Ze Min Huang ),( Yi Tian ),( Jun Tang ),( Yan Hua Zheng ),( Feng Shuo Jin ),( Yan Feng Li ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.8

It is generally accepted that spermatozoa capacitation is associated with protein kinase A-mediated tyrosine phosphorylation. In our previous study, we identified the fibrous sheath CABYR binding protein (FSCB), which was phosphorylated by PKA. However, the phosphorylation status of FSCB protein during spermatozoa capacitation should be further investigated. To this aim, in this study, we found that phosphorylation of this 270-kDa protein occurred as early as 1 min after mouse spermatozoa capacitation, which increased over time and remained stable after 60 min. Immunoprecipitation assays demonstrated that the tyrosine and Ser/Thr phosphorylation of FSCB occurred during spermatozoa capacitation. The extent of phosphorylation and was closely associated with the PKA activity and spermatozoa motility characteristics. FSCB phosphorylation could be induced by PKA agonist DB-cAMP, but was blocked by PKA antagonist H-89.Therefore, FSCB contributes to spermatozoa capacitation in a tyrosine-phosphorylated format, which may help in further elucidating the molecular mechanism of spermatozoa capacitation. [BMB reports 2011; 44(8): 541-546]

Enhancement of light absorption using high-<i>k</i> dielectric in localized surface plasmon resonance for silicon-based thin film solar cells

Li, Hua-Min,Zhang, Gang,Yang, Cheng,Lee, Dae-Yeong,Lim, Yeong-Dae,Shen, Tian-Zi,Yoo, Won Jong,Park, Young Jun,Kim, Hyunjin,Nam Cha, Seung,Kim, Jong Min American Institute of Physics 2011 JOURNAL OF APPLIED PHYSICS - Vol.109 No.9

Identification and evolutionary history of the DD41D transposons in insects

Xiao-Gu Zhang,Hua-Hao Zhang,Yi-Hong Shen,Xiao-Min Xiong,Min-Jin Han 한국유전학회 2016 Genes & Genomics Vol.38 No.2

The rosa monophyletic group of transposons is a group of transposable element with characteristics of encoding a DD41D motif in the catalytic domain. However, biology and evolutionary history of this monophyletic group are still poorly understood. In this study, we report the first description for the presence of a rosa transposon in the silkworm Bombyx mori. Further analyses confirmed that this element in the silkworm genome had recently amplified and might still be capable of transposition. In addition, we present evidence, based on searches of publicly available insect genomes, that a new clade of the rosa monophyletic group was identified. Interestingly, analysis of their three dimensional structures suggested that these proteins showed highly similar protein structures with that of the Mos1 transposase. These results provided useful insights into the functionality of these transposases and their structural and functional deviations from other transposases in the Tc1/mariner superfamily. Meanwhile, sequence and phylogenetic analysis confirmed that DD41D and maT elements might represent another independent large group of the Tc1/mariner superfamily. Importantly, the result of the comparison of terminal inverted repeats (TIRs) validated that DD41D and maT elements almost had identical consensus terminal sequences (50-CAGGGTGNS NCA-30), implying they might have similar cleavage sites or patterns during the process of their transposition. In a word, this study will enrich and expand our knowledge of the Tc1/mariner superfamily.

편액에 함의된 경관 및 상징요소의 출현특성 - 한국의 담양원림과 중국의 소주원림을 중심으로 -

함광민 ( Ham Kwang-min ),이수화 ( Li Shu-hua ),장아평 ( Zhang Ya-ping ),미타니토루 ( Mitani Toru ),장준화 ( Zhang Jun-hua ) 한국전통조경학회(구 한국정원학회) 2016 한국전통조경학회지 Vol.34 No.4

16세기 한국의 담양원림과 중국 소주원림의 편액에 함의된 경관 및 상징요소의 출현특성을 비교분석한 본 연구의 결과는 다음과 같다. 첫째, 편액에 함의된 경관요소 중, 식물요소의 출현율이 양국 원림에서 높게 나타났다. 담양원림의 경우 원림 주변에 조성된 죽림과 자연림이, 소주 원림의 경우 인공적으로 조성한 가산(假山)과 수변 주변의 연, 대나무, 소나무를 포함한 다양한 식물이 편액의 의미에 반영 된 것이 특징이다. 반면, 풍경의 정취를 고조시키는 비, 바람, 달 등을 포함한 기후 및 천체요소는 담양원림의 편액과, 건물과 다리, 책 등을 포함한 인공요소는 소주원림의 편액과 관련되는 것이 특징이다. 이는 산수가 수려한 곳에 찾아 들어가 인공을 최소화하고 자연과의 합일을 미적 이상으로 삼는 한국원림의 특징과, 평탄한 곳에 원림의 경계와 경물을 인공적으로 조성하고 자연과의 동화를 미적 이상으로 삼는 중국원림의 특징을 명확히 하는 근거로 수렴될 수 있겠다. 둘째, 편액에 함의된 상징요소 중, 성인(聖人)의 기본덕목인 도덕적 인품이 두 지방 원림의 사유세계에 있어 보편적 특징으로 나타난다. 반면, 정치안정에 대한 의지는 담양원림의 편액과, 선조를 회고하고 기리는 마음은 소주원림의 편액과 관련되는 것이 특징이다. 이는 사회ㆍ정치적 양상과 연관된 것으로, 당시 호남세력들의 정치적 이상이, 강남 봉건세력들의 유교적 이념이 원림 향유 속에 드러난 것으로 이해된다. 셋째, 담양원림의 편액에 함의된 상징요소 「도덕」과 「정치안정」은 기후와 천체요소가 만들어내는 정경(情景)적 분위기와, 소주원림의 편액에 함의된 상징요소 「도덕」은 식물이 갖는 상징적인 의미와 깊게 관련되어 있고, 이는 한중 원림의 영역을 눈에 보이지 않는 비가시(非可視)영역으로 확장하는 특징과 연결된다. The comparative analysis result of appearance characteristic of landscape elements and symbolic elements implied by the tablets on the 16th century`s Korean Damyang garden and Chinese Suzhou garden is as follows. First, among the landscape elements implied in the tablets, appearance rate of botanical factors appeared high in the gardens of both areas. Damyang garden displayed bamboo grove and natural forest around the garden, while Suzhou garden displayed artificial mountain(假山) created artificially and a variety of plants including lotus, bamboo, and pine surrounding it were associated with the significance of the tablets. On the other hand, climatic/celestial elements including the rain, wind, and the moon were associated with the tablets of Damyang garden, while the artificial factors such as the building, bridge, and book, etc. were mostly were associated with the tablets of Suzhou garden. Second, among the symbolic elements included in the tablets, ethical personality which is the basic virtue of a noble man(聖人), was the universal characteristic of the meditation world of the garden in both areas. However, a will for political stability was mostly associated with the tablets of Damyang garden, while the retrospect heart for the immortal was mostly associated with the signboard in Suzhou garden. It was concerned with political ideal and the thought of Confucianism respectively. Third, the symbolic elements that appeared frequently in the tablets of Damyang garden, 「Ethics」 and 「Political stability」, were associated with the scene atmosphere created by the climatic elements and celestial elements. On the contrary, the symbolic elements which most frequently appeared in the tablets of Suzhou garden, 「Ethics」, was associated with the symbolic significance of the plant. The invisible space of gardens was expanded by tablets in both areas.

Frequency and Temperature Dependence of the Dielectric Properties of a PCB Substrate for Advanced Packaging Applications

Hua-Min Li,,Gang Zhang,이기욱,김재동,Chang-Ho Ra,Won Jong Yoo 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.54 No.3

The frequency- and temperature-dependent dielectric properties for printed circuit board (PCB) applications are investigated in the frequency range from 1 kHz to 1 MHz and the temperature range from 300 K to 600 K. The applicable range of frequency for the capacitance-voltage (C-V) technique is discussed. An analytical model based on the experimental results and the theoretical analysis is proposed to give rise to a comprehensive understanding of the correlations between the operation temperature and the dielectric properties, where the temperature-dependent dipole property is investigated. The frequency- and temperature-dependent dielectric properties for printed circuit board (PCB) applications are investigated in the frequency range from 1 kHz to 1 MHz and the temperature range from 300 K to 600 K. The applicable range of frequency for the capacitance-voltage (C-V) technique is discussed. An analytical model based on the experimental results and the theoretical analysis is proposed to give rise to a comprehensive understanding of the correlations between the operation temperature and the dielectric properties, where the temperature-dependent dipole property is investigated.

Impact of Caspase-8 (CASP8) -652 6N Del and D302H Polymorphisms on Prostate Cancer in Different Ethnic Groups

Zhang, Cheng-Dong,Li, Hong-Tao,Liu, Kun,Lin, Zhi-Di,Peng, Qi-Liu,Qin, Xue,He, Min,Wu, Hua,Mo, Zeng-Nan,Yang, Xiao-Li Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

Background: Despite evidence suggesting roles for caspase-8 (CASP8) -652 6N del and D302H polymorphisms in prostate cancer (PCa), the association of these polymorphisms with PCa risk remains inconclusive. Therefore, a meta-analysis was performed to more precisely estimate the association of CASP8 -652 6N del and D302H polymorphisms with PCa susceptibility. Materials and Methods: A comprehensive literature search was conducted to identify all case-control studies of CASP8 D302H and -652 6N del polymorphisms and PCa risk. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to assess the strength of the association and the precision of the estimate, respectively. Results: Nine -625 6N del studies and 4 D302H studies were included. CASP8 -652 6N del and D302H polymorphisms were not significantly associated with PCa risk in the overall analyses. However, in the subgroup analysis stratified by ethnicity, -625 6N del was significantly associated with PCa risk in the East Asian and Indian populations under the recessive model. Furthermore, the subgroup analysis strongly suggested that D302H was associated with lower PCa risk in the Non-Indian population under the dominant model. Conclusions: In our meta-analysis, ethnic-specific differences were evident in the association of CASP8-625 6N del and D302H polymorphisms with PCa risk.

Estimation of Trapped Charge Density in SONOS Flash Memory Using a Parallel Capacitor Model

Hua-Min Li,Gang Zhang,Won Jong Yoo IEEE 2011 IEEE transactions on electron devices Vol.58 No.10

<P>This paper proposes a model established using the parallel connection of MOS and floating-gate MOS capacitors to examine the electric properties of polysilicon-oxide-nitride-oxide-silicon (SONOS) Flash memory in both the fresh and programmed states. A linear relationship between threshold voltage shift and effective trapped charge density was revealed via the threshold electric field. The simulation of the channel current was given based on our model, being in agreement with the experimental results for both forward and reverse read modes. The model and theory make an easy and time-saving approach to comprehensively analyze the trapped charge and its effect on other electric properties, e.g., electric field, oxide capacitance, and charge distribution, contributing to the control of the write/erase operation, the optimization of the device structure, and the characteristics of the retention properties in SONOS Flash memory.</P>

Influence of Ionizing Radiation on Ovarian Carcinoma SKOV-3 Xenografts in Nude Mice under Hypoxic Conditions

Zhang, Yong-Chun,Jiang, Gang,Gao, Han,Liu, Hua-Min,Liang, Jun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5

Purpose: We aimed to detect the expression of HIF-1${\alpha}$, VEGF, HPSE-1 and CD31 in SKOV3 xenografts in nude mice treated with different doses of ionizing radiation, trying to explore the possible mechanism of hypoxia and radioresistance. Methods: Nude mice bearing SKOV3 xenografts were randomly divided into 4 groups: Group A (control group, no ionizing radiation), Group B (treated with low dose of ionizing radiation: 50cGy), Group C (treated with high dose of ionizing radiation: 300cGy), Group D ( combined ionizing radiation, treated with ionizing radiation from low dose to high dose : 50cGy first and 300cGy after 6h interval). The mRNA levels of HIF-1 and VEGF in each group were detected by real time polymerase chain reaction, while HPSE-1 expression was measured by ELISA. The microvessel density (MVD) and hypoxic cells were determined through immunohistochemical (IHC) staining of CD31 and HIF-1a. Results: Significant differences of HIF-1${\alpha}$ mRNA level could be found among the 4 groups (F=74.164, P<0.001): Group C>Group A>Group D> Group B. The mRNA level of VEGF in Group C was significantly higher than in the other three groups (t=-5.267, P=0.000), while no significant difference was observed among Group A, B and D (t=1.528, 1.588; P=0.205, 0.222). In addition, the MVD was shown to be the highest in Group C (t=6.253, P=0.000), whereas the HPSE-1 level in Group A was lower than in Group B (t=14.066, P=0.000) and higher than in Group C (t=-21.919, P=0.000), and similar with Group D (t=-2.066, P=0.058). Through IHC staining of HIF-1a, the expression of hypoxic cells in Group A was (++), Group B was (+), Group C was (+++) and Group D was (+). Conclusion: Ionizing radiation with lowerdoses might improve tumor hypoxia through inhibiting the expression of HIF-1 and HPSE-1, whereas higherdoses worsen tumor hypoxic conditions by up-regulating HIF-1${\alpha}$, HPSE-1, VEGF and CD31 levels. A protocol of low-dose ionizing radiation followed by a high-dose irradiation might at least partly improve tumor hypoxia and enhance radiosensitivity.

Expression of Tumor Necrosis Factor Receptor-associated Factor 6 in Lung Cancer Tissues

Zhang, Xiu-Ling,Dang, Yi-Wu,Li, Ping,Rong, Min-Hua,Hou, Xin-Xi,Luo, Dian-Zhong,Chen, Gang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Background: Tumor necrosis factor (TNF) receptor-associated factor 6 (TRAF6) has been reported to be associated with the development of various cancers. However, the role of TRAF6 in lung cancer remains unclear. Objective: To explore the expression and clinicopathological significance of TRAF6 protein in lung cancer tissues. Materials and Methods: Three hundred and sixty-five lung cancer samples and thirty normal lung tissues were constructed into 3 microarrays. The expression of TRAF6 protein was determined using immunohistochemistry (IHC). Furthermore, correlations between the expression of TRAF6 and clinicopathological parameters were investigated. Results: The expression of TRAF6 in total lung cancer tissues (365 cases), as well as in small cell lung cancer (SCLC, 26 cases) and non-small cell lung cancer (NSCLC, 339 cases) was significantly higher compared with that in normal lung tissues. The ROC curve showed that the area under curve of TRAF6 was 0.663 (95%CI 0.570~0.756) for lung cancer. The diagnostic sensitivity and specificity of TRAF6 were 52.6% and 80%, respectively. In addition, the expression of TRAF6 was correlated with clinical TNM stage, tumor size and lymph node metastasis in all lung cancers. Consistent correlations were also observed for NSCLCs. Conclusions: TRAF6 might be an oncogene and the expression of TRAF6 protein is related to the progression of lung cancer. Thus, TRAF6 might become a target for diagnosis and gene therapy for lung cancer patients.