A New Spongilipid from the Freshwater Sponge Spongilla lacustris

Jiang-Miao Hu,You-Xing Zhao,Ji-Jun Chen,Ze-Hong Miao,Jun Zhou 대한화학회 2009 Bulletin of the Korean Chemical Society Vol.30 No.5

Chemical Components of Dendrobium polyanthum

Jiang-Miao Hu,You-Xing Zhao,Ze-Hong Miao,Jun Zhou 대한화학회 2009 Bulletin of the Korean Chemical Society Vol.30 No.9

A new tetrahydroanthracene, 3,6,9-trihydroxy-3,4-dihydroanthracen-1(2H)-one (1), six phenolics, moscatilin (2), gigantol (3), batatasin (4), moscatin (5), 9,10-dihydromoscatin (6), 10-dihydrophenanthrene-2,4,7-triol (7), and a sesquiterpenoid, corchoionoside C (8), together with two sterols β-sitosterol (9) and daucosterol (10), were isolated from the stems of Dendrobium polyanthum. Compounds 1 and 2 were assessed for cytotoxic activity against two human tumor cell lines (A549 and HL-60).

A New Spongilipid from the Freshwater Sponge Spongilla lacustris

Hu, Jiang-Miao,Zhao, You-Xing,Chen, Ji-Jun,Miao, Ze-Hong,Zhou, Jun Korean Chemical Society 2009 Bulletin of the Korean Chemical Society Vol.30 No.5

Chemical Components of Dendrobium polyanthum

Hu, Jiang-Miao,Zhao, You-Xing,Miao, Ze-Hong,Zhou, Jun Korean Chemical Society 2009 Bulletin of the Korean Chemical Society Vol.30 No.9

A new tetrahydroanthracene, 3,6,9-trihydroxy-3,4-dihydroanthracen-1(2H)-one (1), six phenolics, moscatilin (2), gigantol (3), batatasin (4), moscatin (5), 9,10-dihydromoscatin (6), 10-dihydrophenanthrene-2,4,7-triol (7), and a sesquiterpenoid, corchoionoside C (8), together with two sterols $\beta$-sitosterol (9) and daucosterol (10), were isolated from the stems of Dendrobium polyanthum. Compounds 1 and 2 were assessed for cytotoxic activity against two human tumor cell lines (A549 and HL-60).

Performance Assessment of InSAR-Based Vertical Displacement Monitoring of Sluices in Coastal Soft Soil Area

Xing Yang,Dongmei Wang,Yongping Xu,Miao Hou,Zhihuan Wang 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.1

Sluices are main flood control infrastructures in coastal areas, where soft rock and soft soil are widely distributed. In soft soil areas, the vertical displacement of sluice is common, which is the main factor affecting the sluice safety. So, it is very important for sluice safety control according to the prototypical observations on deformation. Considering some drawbacks of leveling, such as it can only provide a limited number of points and requires time-consuming field work, this paper evaluates the performance of Interferometric Synthetic Aperture Radar (InSAR)-based method in monitoring long-term vertical displacement deformations of sluices.Taking 12 sluices in Northern Jiangsu, China as an example, 129 Sentinel-1 C-band VV-polarizatio ascending SAR images that span the period from April 2015 to January 2020 were used to monitor their vertical displacement deformations. The leveling results are made to verify the accuracy and reliability of Permanent Scatterer InSAR (PSInSAR) method. The validation indicates good agreement between PSInSAR results and leveling results. Considering the higher accuracy of leveling, PSInSAR method is more suitable as an auxiliary technology besides leveling method to focus on the early warning of sluice deformation risk events.

Rhizospheric fungi of Panax notoginseng: diversity and antagonism to host phytopathogens

Miao, Cui-Ping,Mi, Qi-Li,Qiao, Xin-Guo,Zheng, You-Kun,Chen, You-Wei,Xu, Li-Hua,Guan, Hui-Lin,Zhao, Li-Xing The Korean Society of Ginseng 2016 Journal of Ginseng Research Vol.40 No.2

Background: Rhizospheric fungi play an essential role in the plantesoil ecosystem, affecting plant growth and health. In this study, we evaluated the fungal diversity in the rhizosphere soil of 2-yr-old healthy Panax notoginseng cultivated in Wenshan, China. Methods: Culture-independent Illumina MiSeq and culture-dependent techniques, combining molecular and morphological characteristics, were used to analyze the rhizospheric fungal diversity. A diffusion test was used to challenge the phytopathogens of P. notoginseng. Results: A total of 16,130 paired-end reads of the nuclear ribosomal internal transcribed spacer 2 were generated and clustered into 860 operational taxonomic units at 97% sequence similarity. All the operational taxonomic units were assigned to five phyla and 79 genera. Zygomycota (46.2%) and Ascomycota (37.8%) were the dominant taxa; Mortierella and unclassified Mortierellales accounted for a large proportion (44.9%) at genus level. The relative abundance of Fusarium and Phoma sequenceswas high, accounting for 12.9% and 5.5%, respectively. In total,113 fungal isolates were isolated from rhizosphere soil. They were assigned to five classes, eight orders (except for an Incertae sedis), 26 genera, and 43 species based on morphological characteristics and phylogenetic analysis of the internal transcribed spacer. Fusarium was the most isolated genus with six species (24 isolates, 21.2%). The abundance of Phoma was also relatively high (8.0%). Thirteen isolates displayed antimicrobial activity against at least one test fungus. Conclusion: Our results suggest that diverse fungi including potential pathogenic ones exist in the rhizosphere soil of 2-yr-old P. notoginseng and that antagonistic isolates may be useful for biological control of pathogens.

Law and Early Warning of Vertical Sluice Cluster Displacements in Soft Coastal Soil

Xing Yang,Cong Yuan,Miao Hou,Chengyang Zhou,Yan Ju,Fei Qi 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.2

The vertical displacement, which is the product of natural sources and human activities, is the key factor affecting the sluice safety. This study provides a systematic approach used for analyzing the law and early warning of sluice cluster vertical displacements in coastal soft soil locations. Two important methods, including probability analysis and principal component analysis (PCA), are used to obtain the necessary information in this study. Among them, PCA is mainly used to identify the risk indices during vertical deformations of sluice cluster. As case studies, 27 sluices in a cluster in Northern Jiangsu Province's coastal area in China are chosen and 14 variables related to sluice uplift, settlement and differential settlement deformations are used. The PCA and additional evidence from the sluice deformation law are used to identify three variables as risk indices, including maximum differential settlement (MMDS), maximum cumulative vertical settlement (MCVS) and maximum cumulative vertical uplift (MCVU). This study divides the risk levels into five grades (i.e., Level 1 to Level 5) based on the selected risk indices and determines their risk thresholds based on the in-situ deformation data from 2010 to 2020. In general, the results demonstrate that the newly proposed approach exhibits an acceptable performance. However, the influence of epistemic and aleatory uncertainties on this study is worthy of further discussion in the future.

Effect of Interface on Mechanical Properties and Stamping Formability of Ti/Al Multilayered Composites

Miao Cao,Hui‑Qin Chen,Kun‐Kun Deng,Xing‑Wang Duan,Shuang Liu,Xin Che,Fei Li 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.5

Ti/Al multilayered composites (LMCs) with 3-layers, 5-layers and 7-layers were prepared by hot‐pressing and hot‐rolling. Theeffects of the interface on the mechanical properties and stamping formability of LMCs were revealed. The results indicatethat the TiAl3phases at the interface thicken gradually with the increase of layers. As the layers of LMCs increases, boththe grain size and the basal texture strength of Ti decrease, and Ti possesses a higher Schmid factors (SF) and more uniformSF distribution, which promotes the overall coordinated deformation of LMCs. The elongation (EL) of LMCs graduallyincreases at the expense of the strength with the increase of layers. The stress–strain transfer across the interface, the releaseof residual stress through interfacial microcracks, and the improvement of the interfacial metallurgical bonding strengthare all conducive to the coordinated deformation between Ti and Al layers, thus the strength of LMCs decreases and theEL enhances. Both the yield strength ratio (σs/σb) and plastic strain ratio (r) decrease, while the strain-hardening exponent(n) increases with the increase of layers of LMCs, which are beneficial to improve the stamping formability of LMCs. Thenumber of interface increases with the increase of layers, which hinders crack propagation. Moreover, the microcracks areeasily induced by the brittle TiAl3phase at the interface, and the main cracks deflect obviously and release part energy inthe form of secondary microcracks, thus delaying the failure of LMCs and improving their stamping formability.

Comparative genomic and functional analysis of Akkermansia muciniphila and closely related species

Juyuan Xing,Xiaobo Li,Yingjiao Sun,Juanjuan Zhao,Shaohua Miao,Qin Xiong,Yonggang Zhang,Guishan Zhang 한국유전학회 2019 Genes & Genomics Vol.41 No.11

Background Akkermansia muciniphila is an important bacterium that resides on the mucus layer of the intestinal tract. Akkermansia muciniphila has a high abundance in human feces and plays an important role in human health. Objective In this article, 23 whole genome sequences of the Akkermansia genus were comparatively studied. Methods Phylogenetic trees were constructed with three methods: All amino acid sequences of each strain were used to construct the first phylogenetic tree using the web server of Composition Vector Tree Version 3. The matrix of Genome-to- Genome Distances which were obtained from GGDC 2.0 was used to construct the second phylogenetic tree using FastME. The concatenated single-copy core gene-based phylogenetic tree was generated through MEGA. The single-copy genes were obtained using OrthoMCL. Population structure was assessed by STRU CTU RE 2.3.4 using the SNPs in core genes. PROKKA and Roary were used to do pan-genome analyses. The biosynthetic gene clusters were predicted using antiSMASH 4.0. IalandViewer 4 was used to detect the genomic islands. Results The results of comparative genomic analysis revealed that: (1) The 23 Akkermansia strains formed 4 clades in phylogenetic trees. The A. muciniphila strains isolated from different geographic regions and ecological niches, formed a closely related clade. (2) The 23 Akkermansia strains were divided into 4 species based on digital DNA-DNA hybridization (dDDH) values. (3) Pan-genome of A. muciniphila is in an open state and increases with addition of new sequenced genomes. (4) SNPs were not evenly distributed throughout the A. muciniphila genomes. The genes in regions with high SNP density are related to metabolism and cell wall/membrane envelope biogenesis. (5) The thermostable outer-membrane protein, Amuc_1100, was conserved in the Akkermansia genus, except for Akkermansia glycaniphila PytT. Conclusion Overall, applying comparative genomic and pan-genomic analyses, we classified and illuminated the phylogenetic relationship of the 23 Akkermansia strains. Insights of the evolutionary, population structure, gene clusters and genome islands of Akkermansia provided more information about the possible physiological and probiotic mechanisms of the Akkermansia strains, and gave some instructions for the in-depth researches about the use of Akkermansia as a gut probiotic in the future.

Nonlinear response analysis of variable speed rotor system under maneuvering flight

Xueyang Miao,Junzeng He,Dahai Zhang,Dong Jiang,Jian Li,Xing Ai,Qingguo Fei 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.10

Maneuverability is one of the important tactical and technical indexes of fighter aircraft. In this paper, the finite element method is used to establish a dynamic model of the rotor system that can consider arbitrary maneuvering flight form and rotor speed variation during the flight, and the vibration characteristic of the dynamic model is investigated in detail. In addition, the nonlinear forces caused by bearings and oil film are also considered. The Newmark-β method combined with Newton-Raphson method is adopted to solve the dynamic equations. The influences of speed variation, rolling, pitching, and yawing maneuver loads on the vibration responses of the rotor system are also evaluated.