Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

GT rotary file을 이용한 근관성형법과 충전방법의 비교

강유미,진정희,유미경,이세준,이광원 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.5

The purpose of this study was to compare the shaping time of two shaping methods and the leakage of three different obturation techniques. Ninty three canaled human molar teeth were used. which were randomly divided into two groups of forty teeth each and ten control teeth. After working length determination. the one group was prepared crowndown technique using rotary root canal instruments of GT rotary files .12/20, .10/20, .08/20 and .06/20 taper(Maillefer Instrument SA. Switzerland). The other group was instrumented with Gates Gildden burs(#1, #2 and #3) to coronal preparation and GT rotary files .08/20 and .06/20 taper to apical preparation. Shaping time was measured. After root canals were instrumented, they were divided to three subgroups and obturated as follows: Subgroup 1, obturated with single cone method : Subgroup 2, obturated with lateral condensation : Subgroup 3, obturated with continuous wave technique. Three subgroups were obturated using non-standardized gutta-percha cone(Diadent, Korea, 06 or .08 taper) and AH-26(Dentsply DeTrey, Germany) as a root canal cement. Ten unobturated teeth served as positive and negative controls. After immersion in 2% methylene blue solution for 1 month, the teeth were washed during 24h. The teeth were determineralized in 10% nitric aciid and dehydrated by immersion in 80, 90 and 100% ethyl alcohol. The teeth were finally cleared and stored in 100% methylsalicylate, and apical dye penetration was evaluated under stereomicroscope(Leica M420, LC, U.S.A) at (×) 8.75 magnification. Liner measurement of dye penetration was assessed with the use of digitalized image analysing system (analySIS, GmbH, Germany), The data were analysed statistically using independent T-test and Two-way ANOVA and Tukey test. The result were as follows: 1. In canal prepared with GT^Trotary file, shaphing time taked more than the group of using Gates Gildden drill to coronal preparation without statistical significant (p>0.05). 2. The group of single cone obturation using canal preparation of GT^TM rotary files showed significantly more apical leakage than those of lateral condensation and continuous wave technique regardless of shaping method (p<0.05). 3. The group of single cone obturation using canal preparation of GT^TM rotary files and Gates Gildden drill showed significantly more apical leakage than those of continuous wave technique regardless of shaping method (p<0.05). 4. Regardless of shaping method. The group of continuous wave obturation showed less apical leakage than those of lateral condensation without statistical significance (p>0.05). 5. The group of single cone obturation using canal preparation of GT^TM rotary files and Gates Gildden drill showed more apical leakage than the group of lateral condensation using sane shaping method without statistical significance (p>0.05).

뇌경색 환자에 있어 시간경과에 따른 영상변화

강경숙,양동선,김진미 국립서울정신병원 2000 臨床硏究論文集 Vol.2000 No.-

자궁경부 세포 인식을 위한 가변 블록 기반 특징 추출 기법

강미영,하진영,김백섭,이헌길 강원대학교 정보통신연구소 2001 정보통신논문지 Vol.5 No.-

Most automated cervical cancer screening systems are recognized through the feature extraction process by segmenting the cell image. There are problems such that an efficient segmentation of the cell image is difficult, and segmentation and feature extraction process takes long time. To solve these problems, PapNet system based on the artificial neural network was developed. However, it takes long time to recognize because huge amount of pixel-based features is inputted into neural network. In this paper, we propose a feature extraction technique which is based on the variable size block and makes use of the color information. The proposed technique can reduce running time by decreasing the input number of neural network by dividing the region of the cell around the center into blocks. This technique shows the low false negative error ratio and the high recognition ratio by color clustering which makes use of the position information and the similarity among homogeneous cells, and by variable size block in proportion to importance ratio of the information.

태극권 수련이 중년여성의 심리적 행복감에 미치는 영향

강현숙,김백윤,박미진,신영호 한국스포츠리서치 2005 한국 스포츠 리서치 Vol.16 No.2

The purpose of this study was to explore the effects of Taichichuan training on the variables of cognitive-affective states. The subjects of the study consisted of 22G middle aged women in Jeonju areas. The survey methods of this research are the 5 Likert Scale modified the Cognitive Affective States Scale by Yang. Myung-Hwan(1998). The used statistical methods for the data analysis were M. SD. Paired sample T-Test. The results obtained by the appropriate analytic processes were followed. 1. In hedonic enjoyment, Taichichuan training women were lower than non-activity women. 2. In feeling of flow, Taichichuan training women were higher than non-activity women. 3. In self-realization, Taichichuan training women were higher than non-activity women. 4. In self-confidence, Taichichuan training women were higher than non-activity women.

공동주택 사전성능평가를 위한 성능평가 시스템 개발에 관한 연구

강미선,이상현,김하나,엄혜진 대한건축학회 2003 대한건축학회논문집 Vol.19 No.10

The purpose of this study is to propose an evaluation framework for Collective Housing and to develop the Design Evaluation System. The Performance Evaluation System of Collective Housing in the Design Development Stage evaluates its design results by running CAD program. The results are automatically reflected in the design process. The scope of this study will be limited to the contents within the Barrier Free Design. Running the design evaluation program requires the conversion of design forms and design checklist to computerized ones. To efficiently conduct this process, First, organize the existing guideline of the Barrier Free Design. Second, define attributes of each part in Building Data Model with the analysis of the checklist. Third, develop the Performance Analysis agent that compares Building Data Model with translated checklists. Finally, verify the efficiency of the Performance Evaluation System by seeking experts' advice on the result of the program.

윤상 다륜형 아급성 피부 홍반성 루푸스 1예

강민정,박혜진,조소연,이미애,황규광,함정희 대한피부과학회 1999 대한피부과학회지 Vol.37 No.6

We report an annular type of subacute cutaneous lupus erythematosus(SCLE) which is rare in Korea, A 23-year-old female had erythematous annular rashes with a mild itching sensation on the face, trunk and both extremities. She also had painless oral mucosal ulceration, diffuse hair loss and mild arthralgia. Serologic abnormalities of the patient were positive anti-Ro Ab, ANA and Anti-ds-DNA Ab. The histopathologic findings showed vacuolar degenerations in the basal cell layer, mononuclear cell infiltrate in the upper dermis, and dermal edema. Direct IF revealed granular deposition of IgG and IgA at the dermo-epidennal junction.

중환자의 욕창 예방 연구 : 욕창 예방 QI팀을 중심으로

강소영,최은경,김진주,주미정 한국의료QA학회 1997 한국의료질향상학회지 Vol.4 No.1

Background : A pressure sore was defined as any skin lesion caused by unrelieved pressure and resulting in damage to underlying tissue. The health care institutions in the United States were reported the incident rate of pressure sores ranging from 6 to 14%. United states were reported the incident rate of pressure sores in patients rate of pressure sore. Also, Annual expenditures for the care of pressure sores in patients in the United States have been estimated to be $7.5 billion; furthermore, 50 percent more nursing time is required to care for patients with pressure sore in comparison to the time needed to implement preventive measures against pressure sore formation. However, In Korea, there were little reliable reports, or researches, about incidence rates of pressure sore in health care institution including intensive care unit and about the integrated approach like CQI action team for risk assessment, prevention and treatment of pressure ulders. Therefore, this study was to develop pressure sore risk assessment tool and the protocol for prevention of pressure sore formation through CQI action team activities, to monitor incident rate of pressure sore and the length of sore formation for patients at high risk, and to approximately estimate nursing time for sore dressing during research period as the effect of CQI action team. Method : CQI action team in intensive care unit, launched since early 1996, reviewed the literature for the standardized risk assessment tool, developed the pressure sore assessment tool based on the Braden Scale, tested its validity, compared on statistics including incidence rate of pressure sore for patients at high risk. Throughout these activities, CQI action team was developed the protocol.called as St. Marys hospital Intensive Care Unit Pressure Sore Protocol, shifted the emphasis from wound treatment to wound prevention, After applied the protocol to patients at high risk, the incident rate and the period of prevention against pressure development were tested with those for patients who received care before implementation of protocol by Chi-square and Kaplan-Meier Method of Survival Analysis. Result : The CQI action team found that there was significant difference of incidence rate of pressure sores between patients at high risk( control group) who received care implementation of protocol (p<.05). 25% possibility of pressure sore formation was shown for the patients with 6th hospital day in ICU in control group. In experimental group, the patients with 10th hospital day had 10% possibility of pressure sore. Therefore, there was significant difference(p< .05) in survival rate between two groups. Also, nursing time for dressing on pressure sore in experimental group was deceased as much as 50% of it in control group. Conclusion : The collaborative team effort led to reduced incidence, increased the length of prevention against pressure sore, and declined nursing care times for sore dressing. However, there have had several suggestions for future study. The preventive care system for pressure sore should be appleed to patients at moderate, or low risk throughout continuous CQI team activities based on Bed sore Indicator Fact Sheet. Hospital-wide supports, such as incentives, would be offered to participants for keeping strong commitment to CQI team. Also, Quality Information System monitoring incidents and estimation cost of poor quality. like workload(full time equivalence) or financial loss, regularly in a hospital has to be developed first for supporting CQI team activities as well as empowering hospital-wide QI implementation. Being several limitations, this study would be one of the report cards for the CQI team activities in intensive care unit of an acute hospital and a trial of quality improvement of health care in Korea.

학습자의 평가 참여를 지원하는 웹 기반 수행평가 시스템의 설계 및 구현

강공미,김진호 한국컴퓨터교육학회 2003 컴퓨터교육학회 논문지 Vol.6 No.3

수행평가는 학생들의 학습 과정을 관찰하거나 학생들이 산출해 낸 자료를 통해 평가하는 방식으로 단순 필답 고사의 단점을 보완하는 새로운 평가 방식으로 주목받고 있다. 그러나 이 수행평가를 적용하는데 많은 노력과 시간 부담이 들어 실제 학교 현장에서 시행에 많은 어려움을 겪고 있다. 이러한 부담을 줄이기 위해 인터넷을 이용한 평가가 다방면에서 시도되고 있으나, 이들 기존 연구에서는 학생의 평가 참여가 허용되지 못하며 평가 방법이 다양하지 못하거나 교사의 피드백이 부분적으로만 사용되는 문제점을 가지고 있다. 따라서 본 논문에서는 학습자들의 평가 참여 및 학습 과정 중의 다양한 수행평가를 지원하고 교사가 효과적으로 관리할 수 있는 웹 기반의 수행평가 시스템을 설계하고 구현하였다. 이러한 웹 기반 수행평가 시스템을 활용할 경우, 학생들의 평가력 등의 고등사고 능력과 전인적 발달을 도모하고, 수행평가를 위한 교사의 업무와 시간 부담을 대폭 줄일수 있을 것이다. A performance evaluation, which requires to observe students in the course of teaming and studying and to evaluate their reports and materials, is emerging as an alternative evaluation method to overcome the shortcoming of simple written tests. However, there are many difficulties in real teaching setting to apply the performance evaluation, because it requires many burdens of efforts and time. In order to reduce these burdens of teachers, there have been several approaches which utilize the Internet for the evaluation. But these previous approaches have several limitations that they don’t allow students’ participation in evaluation activities, fail to provide a variety of evaluation methods, and/or support teachers’ feedbacks very limitedly. In order to overcome these limitations, therefore, this paper designed and implemented a web-based performance evaluation system supporting the participation of students in doing evaluation themselves and various evaluation methods, which can be effectively managed by teachers. This web-based performance evaluation system developed in this paper can enhance not only students’ high level thinking abilities but also their emotional and intellectual abilities. It can also help teachers to reduce the burden of working and the time in plenty used for evaluating students.