http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Kang, Mi Lan,Kwon, Jin Seon,Kim, Moon Suk Molecular Diversity Preservation International (MD 2013 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.14 No.4
<P>Several studies have demonstrated that basic fibroblast growth factor (bFGF) can induce neural differentiation of mesenchymal stem cells. In this study, we investigated the neural differentiation of muscle-derived stem cells (MDSCs) following treatment with bFGF and ethosuximide, a small molecule used as an anticonvulsant in humans. Stem cells isolated from rat skeletal muscle (rMDSCs) were pre-induced by culturing with 25 ng/mL bFGF for 24 h and then were transferred to a medium supplemented with or without 4 mM ethosuximide. Neuronal differentiation was assessed by immunocytochemical and western blotting analyses of marker expression. Immunocytochemistry of rMDSCs treated with bFGF and ethosuximide identified abundant cells expressing neuronal markers (TuJ1, neuron-specific class III β-tubulin; NeuN, neuronal nuclear antigen; and NF-MH; neurofilament M and H). Olig2 (oligodendrocyte transcription factor 2)-positive cells were also observed, indicating the presence of oligodendrocyte lineage cells. These findings were substantiated by western blotting analysis of marker proteins. In particular, the expression of NeuN and TuJ1 was significantly higher in rMDSCs treated with ethosuximide and bFGF than in cells stimulated with bFGF alone (NeuN, <I>p</I> < 0.05 and TuJ1, <I>p</I> < 0.001). Expression of the astrocyte marker GFAP (glial fibrillary acidic protein) was not detected in this study. Collectively, the results showed that treatment with bFGF and ethosuximide induced effective transdifferentiation of rMDSCs into cells with a neural-like phenotype. Notably, rMDSCs treated with a combination of bFGF plus ethosuximide showed enhanced differentiation compared with cells treated with bFGF alone, implying that ethosuximide may stimulate neuronal differentiation.</P>
Kang, Mi-Lan,Kim, Ji-Eun,Im, Gun-Il Wiley (John WileySons) 2017 JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MED Vol.11 No.12
<P>Vascular endothelial growth factor (VEGF)-transfected adipose-derived stromal cells (ADSCs(VEGF)) were devised to promote bone regeneration and neovascularization of bone marrow stromal cells (BMSCs). ADSCs(VEGF) were added to BMSCs and cocultured in variable proportions. ADSCs(VEGF) alone or ADSCs(VEGF) with BMSCs (BMSCs:ADSCs(VEGF) ratio of 1:0.025-0.5) induced significantly greater tube formation in human umbilical vein endothelial cells than untransfected ADSCs. The cocultures of BMSCs and ADSCs(VEGF) at ratios of 1: 0.025-0.1 showed significantly greater osteogenic differentiation and mineralization than BMSCs alone in vitro. Osteogenic markers COL1A1, OCN and BSP were most effectively induced at the BMSC: ADSC(VEGF) ratio of 1:0.05. Of angiogenesis-related genes, upregulation of cathepsin Z and downregulation of early growth response 1 were observed while two osteogenesis-related genes, osteoactivin and tetranectin, were upregulated in BMSCs/ADSCs(VEGF) compared to BMSCs/ADSCs. When critical size calvarial defects in rats were implanted with mixture of BMSCs and ADSCs(VEGF) along with hydroxyapatite/-tricalcium phosphate granules, BMSCs and ADSCs(VEGF) at the ratio of 1:0.05 showed better bone regeneration that BMSCs alone. The cotransplantation of ADSCs(VEGF) with BMSCs significantly increased neovascularization on the regenerated bone of the repaired defect than BMSCs alone. In conclusion, ADSCs(VEGF) added in small proportion to BMSCs effectively promote bone regeneration and neovascularization. Copyright (c) 2017 John Wiley & Sons, Ltd.</P>
Kang, Mi Lan,Shin, Seung Won,Rayamahji, Nabin,Seo, Yeon Soo,Lee, Su In,Lee, Won Hyung,Yoo, Han Sang The Korean Society of Veterinary Science 2008 大韓獸醫學會誌 Vol.48 No.1
We have investigated efficiency of a recombinant subunit Pasteurella multocida toxin (PMT) that was mixed with a vaccine consisted of inactivated whole cells of Bordetella bronchiseptica, P. multocida (types A and D). For verification of the efficacy of the vaccine, all experimental pigs (suckling piglets, sow and gilts) in the three farms were vaccinated. Antibody titers against B. bronchiseptica and P. multocida type A of the vaccinated pigs by microplate agglutination were significantly higher than those of the control pigs (p < 0.05). Similar patterns were observed in the analysis of anti- PMT neutralizing antibody by serum neutralizing method using Vero cell (p < 0.05). Anti- P. multocida type D antibody titer of the vaccinated sows and gilts by ELISA showed significant differences with those of the non-vaccinated pigs (p < 0.05). Although antibody titers increased, it was unable to find out the difference in the clinical signs between the vaccinated and non-vaccinated pigs. However, the increase in body weight of the vaccinated piglets was observed in comparison with the non-vaccinated piglets on a farm. At slaughtering of the pigs, pathological lesions in the turbinate bones of the vaccinated pigs were significantly lower than those of the non-vaccinated pigs (p < 0.001). These results suggested that efficacy of the vaccine in pigs demonstrated to protect against atrophic rhinitis in Korea.
Potential induction of rat muscle-derived stem cells to neural-like cells by retinoic acid
Kim, E Sle,Kim, Gyeong Hae,Kang, Mi Lan,Kang, Yun Mi,Kang, Kkot Nim,Hwang, Ki Chul,Min, Byoung Hyun,Kim, Jae Ho,Kim, Moon Suk Wiley (John WileySons) 2011 JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MED Vol.5 No.5