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Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression
Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11
<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>
송미숙,전기홍,송현종,김정은,박승구,신상건,이동훈,정성현,최연주,최완철,최민규,황재철,홍지만 아주대학교 1997 아주의학 Vol.2 No.2
This study was performed to identify risk factors for depression among the elderly in a rural area. A survey was done for the elderly living in Gosam-myenn, Ansung-kun during the period of July 28, 1997 and July 30, 1997. Demographic and sodoeconornic characteristics, and functional and health Status were assessed using a structured questionnaire, and the level of depression was measured by GDS score. The data obtained were analyzed by frequencies, percentage, t-test, ANOVA, and multiple regression, and the results were as follows: 1. The total sample consisted of 133 elderly. Of them, 47 elderly (35.4%) showed significant depressive Symptoms. 2. The GDS score was the highest for the group over 85 years of age (p < 0.05). Those living alone or widowed showed lower GDS score than those living with someone or married (p < 0.05). 3. The GDS score was the highest in the absolutely dependent group, followed by the partially dependent group, and the independent group (p < 0.01). The elderly who perceived their health Status as excellent showed the lowest GDS score. In contrast, those who had more than five chronic diseases showed the highest GDS score. 4. The elderly receiving financial and emotional support from their family or friends showed lower GDS score compared to those without any support. 5. The economically dependent group showed higher GDS score than that of independent group. 6. The elderly who involved in leisure activities showed lower GDS score than those without any involvement. 7. In terms of risk factors for depression, we found that perceived health Status, subjective economic Status, leisure activities, living arrangement and financial support were inversely associated with GDS score, whereas labor support was positively associated with GDS score. Based upon the above findings, this study suggests that these risk factors for depression in the elderly should be taken into consideration for a comprehensive mental health program for the elderly living in the rural area.
한의진단명과 진단요건의 표준화 연구 II (표준화 실례) : 2차년도 연구결과 중간 보고
양기상,최선미,최승훈,안규석,박경모,박종현,김성우,신승호,정우열,전병훈,고현,김정범,신상우,김성훈,김동희,권영규,엄현섭,장혜옥 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1
The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the second year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows; - differential diagnosis according to condition of body fluid, differentiation of syndromes according to the state of qi and blood, differential diagnosis according to relative excessiveness or deficiency of yin and yang(氣血陰陽津液辨證) - differentiation of diseases according to pathological changes of the viscera and their interrelation - analyzing and differentiating of febrile diseases in accordance with the theory of the six channels(傷寒辨證) The individual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name(異名), notion of diagnosis pattern, index of differentiation of symptoms and signs(辨證指標), the main point of diagnosis, analysis of diagnosis pattern(證候分析), discrimination of diagnosis pattern(證候鑑別), a way of curing a diseases(治法), prescription(處方), herb in common use(常用藥物), diseases appearing the diagnosis pattern(常見疾病), documents(文獻調査). This study was carried out on the basis of the Chinese documents and references.
청주시 소비자의 그린마케팅에 대한 인식과 그린제품 구매의도
권소영, 박혜영, 최혜미, 최현미, 유현정 충북대학교 생활과학연구소 2013 생활과학연구논총 Vol.17 No.2
The purpose of this study was to analyze consumers' perception of green marketing and purchase intention of green products. Questionnaire survey was conducted from 9 August to 29 August 2010. We have surveyed 298 consumers who were living in Cheongju area. Major findings are as follows. First, consumers' environment perception and involvement, awareness of green marketing, evaluation for green products, purchase intention of green products were generally positive. It means that Korean consumers have a positive attitude and behavioral intention for green consumption. Second, consumers responded high perception level for necessity of green marketing and purchase intention of green products. On the other hand, consumers think that green product manufacturers need to more protect our environment. Consumers evaluated the quality of green products is good but its price is high. Third, insufficient information was the most common response of the items asked for the resistance of the whole process for the production of green products. For benefit part, high quality and symbolic value of green consumer were given answer highly. Fourth, there were differences among the consumer groups from environment perception, awareness of green marketing, evaluation for green products, and purchase intention of green products by consumers' characteristics. Old aged group were high involved on environment and student group showed relatively low environment perception and involvement of green consumption. Four million won or less income groups were relatively higher interest in green products and eco-conscious consumer groups have a high degree of purchasing intention of green products.
Zinc protoporphyrin과 다양한 용매조건이 MTT formazan의 발색 안정성에 미치는 영향
박경아,최유미,최현아,김미리,김현정,홍정일 서울여자대학교 자연과학연구소 2011 자연과학연구논문집 Vol.23 No.-
An assay method using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltertrazolium bromide (MTT) is commonly utilized for analyzing cell viability. In this method, MTT tetrazolium is converted to formazan by mitochondrial reductase of viable cells. The formzan formed is, however, not stable; the stability is affected by various factors. In the present study, effects of zinc protoporphyrin (ZnPP) and different solvents on color response of formazan dye were investigated. MTT formazan was stable under dark; light enhanced the color degradation of formazan. ZnPP induced rapid degradation of formazan under light condition in DMSO solution. Decolorization of formazan was faster in aqueous solvents, including distilled water, HCl as well as Triton X-100 solution. Formazan dye was most unstable in HCl (0.1 N) regardless of light or presence of ZnPP. Color response of formazan dye in NaOH (0.1 N) was enhanced by ~2 folds as compared to one in DMSO. MTT formazan was stable in this condition; the color change was less than 10% even under light during 2 hr. ZnPP, however, induced rapid decolorizaion of formazan dissolved in NaOH under light. H2O2, an oxidizing agent, did not affect color response of MTT formazan. The present results suggest that various factors could affect the color response of MTT formazan, which needs to be considered carefully in MTT assay. Key words:MTT tetrazolium, formazan, zinc protoporphyrin, cell viability, sodium hydroxide.
국가 음악과 교육과정에 기초한 초·중등학교 음악예술프로그램 적용
석문주,권덕원,최은식,최미영,김명숙,박장미,손미현,정지혜 이화여자대학교 사범대학 교육과학연구소 2011 교육과학연구 Vol.42 No.2
In order to increase opportunities for students to enjoy the musical arts, it is necessary to develop musical arts programs that are based on students’ interests and the national (or provincial) curriculum, that can be systematically and effectively supported by teachers. In this regard, the purpose of this study was to develop those musical arts, carry out the programs in one elementary school and one middle school from October to December 2011, and analyze each implementation. For the analysis, lesson videos, students’ worksheets, in-depth interviews with students, teachers, and teaching artists, and lesson plans were collected. The conclusions and implications are as follows: 1) the role and meaning of musical arts programs based on national curriculum were more highlighted; 2) students could have great depth of aesthetic experience by the means of direct contact with professional musicians and concrete musical experience; 3) the role of music mediators and its importance was magnified; 4) there was a positive change in the musical preferences and attitudes of students with regard to the musical artsprograms. 본 연구는 학생들의 음악 예술 향수기회 확대를 위해 학생들의 흥미와 국가 교육과 정을 중심으로 하는 ‘현장성’, 교사들의 음악 교육을 효과적으로 지원할 수 있는 ‘체계성’, 그리고 음악 예술 전문가들과 학교 사이에 꾸준히 실행될 수 있는 ‘지속성’을 갖춘 음악예술프로그램을 2010년 10월부터 12월까지 초등학교와 중학교에 적용하고 그 결과를 분석하고자 하는데 목적이 있다. 본 연구는 수업 동영상, 학생 활동지, 학생과 교사 심층인터뷰, 강사 인터뷰, 수업안 자료를 수집하여 분석하였다. 본 연구의 결론은 첫째, 국가 교육과정을 반영한 음악 예술프로그램의 역할과 의미가 더욱 확장되었다. 둘째, 음악가들과의 직접적인 만남과 구체적인 예술적 체험을 통하여 학생들은 이전과 비교될 수 없는 ‘깊이 있는 미적 경험’을 하게 되었다. 셋째, 음악메디에이터의 역할이 부각되었다. 넷째, 음악예술프로그램을 통하여 학생들에게 음악에 대한 삶의 변화가 있었다.
Rat의 DNA Polymerase β cDNA가 도입된 Transgenic Drosophila의 체세포 돌연변이 유발에 관한 연구
최영현,유미애,이원호 부산대학교 유전공학연구소 1995 분자생물학 연구보 Vol.11 No.-
The effects of DNA polymerase β on the somatic chromosome mutations and mitotic recombinations were investigated using the transgenic Drosophila bearing chimeric gene consisting of a promoter region of Drosophila actin 5C gene and rat DNA polymerase β. For detecting the somatic chromosome mutations and mitotic recombinations, the heterozygous (mwh/+) strains possessing or lacking transgene pel β were used. The spontaneous frequency of small mwh spots, due deletion or nondisjunction etc, in the non-transgenic w strain and the transgenic p[pol β]-130 strain was 0.351 and 0.606, respectively. The spontaneous frequency (0.063) of large mwh spots, arises mostly from somatic recombination between the centromere and the locus mwh, in the transgenic p[pol β]-130 strain was about three times higher than that (0.021) of the non-transgenic w strain. The mutant clone frequencies of small and large mwh spots induced by N-methyl-N'-nitro-N-nitrosoguanidine and ethyl methanesulfonate in the transformant p[pol β]-130 were higher than those in the host strain w. The present results suggest that rat DNA polymerase β participate at least in the somatic chromosome mutations and mitotic recombination processes.
최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1
This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.
관개수질을 고려한 시비가 벼의 생산성과 농업환경에 미치는 영향
엄미정,박현철,김갑철,류정,최정식 한국환경농학회 2004 한국환경농학회지 Vol.23 No.1
관개수 수질을 고려한 감비가 벼의 생산성과 농업환경에 미치는 영향을 분석한 결과는 다음 과 같다. 시험에 사용된 관개수의 질소, 인, 칼륨의 평균함량은 각각 6.16, 0.26, 9.37 ㎎/L 이 었고, 유입된 영양염류가 논을 통과하면서 농도가 감소되어 유출수의 성분은 유입된 관개수 보다는 낮은 농도를 보였으며, 논 표면수와 침출수의 농도는 시비량과 시비시기에 따라 변 화하였다. 토양검정 50% 시비구에서는 질소의 공급량과 소비량간의 차이가 0.14㎏/ha으로 나타나 양분수지면에서 균형을 이룬 반면, 농가관행시비구에서는 그 차이가 95.3㎏/ha로 나 타났다. 농가관행시비구에 비해 토양검정시비구와 토양검정50% 시비구는 등숙률, 천립중, 도정특성, 등이 우수하여 완전미수량에서 큰 차이를 보이지 않았으며, 시비량이 적을수록 단 백질 함량이 또한 적어 기계적인 식미치가 높게 나타나 품질에서 우수하였다. 시비효율에서 는 토양검정50% 처리에서 시비된 성분이 수량에 기여하는 비율이 높아서 시비이용과 효율 면에서 우수한 결과를 보였다. 이상과 같이, 관개수에 포함된 영양물질을 작물의 생육에 이 용함으로서, 농업용수의 수자원 이용효율을 높이는 동시에, 비료 절감효과와 생산량 증대의 가능성을 검토할 수 있었다. Table 11.Physicochemical properities and paratability of milled rice in the different fertilization ▷표삽입◁ (원문을 참조하세요) This study was conducted to investigate the effect on agricultural environment and crop productivity by different amount of applied fertilizer in consideration of irrigation water quality. N, P and K contents of irrigation water used in this eqdment were 6.16, 0.26 and 9.37 ㎎/L, reqecthly. N, P and K Concentrations of runoff water were lower than those of inflow water during rice cultivation. N, P and K Concentrations of ponded and percolated water were changed according to the amount and time of applied fertilization. During rice cultivation in paddy soil, nitrogen balance was closed to 0 in STF 50% (50% level of sail testing fertilizaton), 0.14 ㎏/ha, but it was 95.3 ㎏/ha in CF (conventional fertilization) treatment in STF 50% and STF (sail testing fertilization) treatment, yield of perfect rice was not greatly different as compared with CF treatment, due to the superiority of ripening rate, 1,000 grains weight and milling characteristics. Mechanical paratability of rice was excellent in NF (non fertilization) treatment, STF 50% treatment showed higher in nutrient availabiilty and fertilizers use efficiency than other treatments.
數 種 韓藥의 아세틸콜린에스터레이즈 酵素活性에 대한 효과
최재환,김미영,김현미,최훈,전훈,김대근,임종필,임강현 대한본초학회 2002 大韓本草學會誌 Vol.17 No.1
아세틸콜린에스터레이즈(AChE)는 신경조직에 분포하는 효소로 신경전달물질 중 아세틸콜린(ACh)을 분해하는 효소로 시냅스 신호전달체계에 있어 중요한 역할을 하는 효소이다. 현재 알츠하이머병과 치매치료에 사용하는 서양약물은 AChE의 활성을 저해하는 작용기전을 가진다. 목적 : 전통적으로 기억력 감퇴, 치매 등 치료에 사용되어 온 한약의 AChE에 대한 작용을 밝히기 위해 본 실험이 구상되었다. 방법 : 생쥐 대뇌신경세포의 균질 혼합액으로부터 효소의 활성을 Ellman 방법을 개선한 엘리자(ELISA)법을 이용하여 측정하였다. 결과 : 실험결과 天花? 의 6종이 2.5㎎/㎖의 농도에서 우수한 효소활성저해력을 보였고, 50% 효소활성 저해농도(ID_50)를 구한 결과, 天花?의 ID_50은 1.5㎎/㎖이었고, ??? 1.2 ㎎/㎖, ?? 2.0 ㎎/㎖, ??草 1.2 ㎎/㎖, ??? 1.0 ㎎/㎖, ??生 1.7㎎/㎖, 및 大?皮 2.0 ㎎/㎖를 나타내어 忍冬?의 저해력이 가장 우수하였다. 결론 : 치매 및 기억력감퇴 증상을 치료하는 한약의 작용기전 중 AChE 효소활성억제효과는 忍冬? 의 6종에서 나타났으며, 이는 전통적인 한방치료에 과학적인 기초자료를 제공할 것으로 생각한다.