Head-Space SPME에 의한 생체시료 중 메스암페타민류 분석(Ⅲ)

박미정,최상길,손행자,최화경,최영남,허상철,박종서,정희선 內務部 國立科學搜査硏究所 2002 國立 科學 搜査 硏究所 年報 Vol.34 No.-

중합효소연쇄반응으로 조사한 관절염 환자의 관절액에서 Tropheryma whipplei DNA 검출

김성연,정문현,최새로운,김은실,이진수,박원,김미경,강재승 대한감염학회 2005 감염과 화학요법 Vol.37 No.2

목적 : Whipple병은 Tropheryma whipplei에 의한 전신적인 감염 질환으로 소장과 관절 침범이 특징이며, 흔히 병의 초기에는 수년 동안 다른 증상 없이 관절염 소견만 보이므로, 일부 환자는 염증성 관절염으로 진단받을 가능성이 있다. 또한 류마티스 관절염을 포함하여 여러 염증성 관절염에서는 아직 병인이 밝혀지지 않았지만 감염이 관련될 가능성은 꾸준히 언급이 되었다. 저자들은 국내에서도 T. whipplei가 존재할 것이라는 가정 하에 한국인에서 중합효소연쇄반응으로 T. whipplei DNA를 조사하였다. 재료 및 방법 : 대상 환자군은 1997년부터 2002년 사이에 인하대병원 류마티스 내과에 관절 종창으로 내원한 환자들로, 류마티스 관절염 환자 33명, 골관절염 환자 18명, 기타 질환에서 관절 이상인 환자들로 5명이 포함되었다. 건강한 성인 8명의 타액과 22명의 위내시경을 받은 환자의 위액을 채취하여 검사하였다. 중합효소연쇄반응은 T. whipplei heat shock protein의 일부 유전자를 증폭할 수 있는 2개의 시동체를 사용하였다. 표준 균주를 얻기가 어려워 양성 대조군 없이 중합효소연쇄반응을 시행하였다. 결과 : 관절염 환자의 관절액, 정상인의 타액, 위내시경을 받은 사람의 위액에서 모두 중합효소 연쇄반응으로 핵산을 증명할 수 없었다. 결론 : 양성대조군이 없어 검사 잘못일 가능성도 배제할 수 없으나, Whipple병이 염증성 관절염의 흔한 원인이 아니거나 관절의 직접 침범보다는 위장관 감염 후 생기는 면역 반응에 의한 반응성 관절염일 가능성이 있다. 관절액 이외의 검체에서 조사한다면 다른 결과를 얻을 수도 있으리라 생각하며 앞으로 이들에 대한 조사가 필요하다. Background : Whipple's disease is a chronic systemic infection caused by Tropheryma whipplei which chiefly involves gastrointestinal tract and joints. Because arthritis is often the first sign of Whipple's disease, Whipple's disease may be misdiagnosed in the early stage of illness as non-infectious inflammatory arthritis. Rheumatoid arthritis may also be caused by infectious agents, including T. whipplei. To elucidate the relationship between T. whipplei infection and various arthritis, we examined T. whipplei DNA in Korean patients by polymerase chain reaction (PCR). Materials and Methods : With two T. whipplei-specific primers dervied from the partial heat shock protein 65 gene sequence, PCR was performed on 56 joint fluid samples : 33 samples from RA patients, 18 samples from OA patients, and 5 samples from patients with other unflammatory arthritis. In addition, the same method was applied to find out the DNA of T. whipplei in 8 normal adults' saliva and 22 patients' gastric juice. Due to the difficulty in obtaining the reference strain of T. whipplei, the PCR was performed without the positive control. Results : The PCR method did not reveal T. whipplei in joint fluid from 56 patients with arthropathies, 8 samples from saliva, and 22 samples from gastric juice. Conclusion : Though technical errors can not be excluded as a cause of absence of a positive case, our results suggest that T. whipplei infection may not be a common cause of various arthritis in Korea; T. whipplei may be associated with inflammatory arthritis indirectly by immune-mediated mechanism rather than by direct joint invasion. The examination of specimens other than joint fluid could be helpful and the study of larger number of patient is highly desirable.

안면비대칭을 동반한 Angle Ⅲ급 부정교합자의 안모형태에 관한 두부방사선계측학적 연구

손우성,강정숙,김미경,김종렬 대한치과교정학회 1994 대한치과교정학회지 Vol.24 No.4

The purpose of this was three-fold: i) to investigate the degree of asymmetry in Angle's Class Ⅲ malocclusion patients and normal adults; ⅱ) to determine the nature of difference existed between two groups; and ⅲ) to investigate the correlationship between the degree of asymmetry and ANB and overbite in Angle's Class Ⅲ malocclusion patients. The subjects consisted of 25 Angle's Class Ⅲ malocclusion patients and 25 normal adults and the mean ages were 22.0 and 24.5 years, respectively. Their posteroanterior and lateral cephalograms were traced and analysed with three-dimensional approach. The results were as follows: 1. Asymmetry of Angle's Class Ⅲ malocclusion group was significant in all regions except cranial base. Their horizontal asymmetry was seen in mandibular angle, maxillary and mandibular midline and menton. Vertical asymmetry was observed in maxillary lst molar and mandibular shape and anteroposterior asymmetry in mandibular angle. 2. Nine variables indicating asymmetry were selected and each variable had similar discriminant score. 3. There was a little correlationship between ANB and asymmetric variable(MSR-B6) and its correlation coefficients was 0.3564. 4. There was no significant correlationship between overbite and asymmetric variables.

서목태(Rhynchosia nulubilis) 추출물이 고지방 및 고콜레스테롤 식이 급여 흰쥐의 혈청 지질 농도에 미치는 영향

신미경,한성희 한국식생활문화학회 2002 韓國食生活文化學會誌 Vol.17 No.1

The effects of the Rhynchosia nulubilis extracts on serum lipid concentrations were evaluated in rats. Forty-two male Sprague-Dawley rats weighing 100±10g were divided into six groups and fed high fat and high cholesterol diets for four weeks. Experimental groups were administered with following diets; basal fat diet(control) and basal and high fat diets with 1% cholesterol and Rhynchosia nulubilis extract(40㎎/㎏, 400㎎/㎏ and 4000㎎/㎏). The concentrations of serum triglyceride in rats fed the Rhynchosis mulubilis extract, high fat, 1% cholesterol, and RN-HFC(-40, -400, 4000㎎/㎏) groups were lower than those in other groups. The concentrations of total cholesterol in the RN-HFC (-40, -400, -4000㎎/㎏) groups were lower than those in fat diet groups. The concentrations of HDL-cholesterol in serum of the RN-HFC(-40, -400, 4000㎎/㎏) groups were significantly higer than those of other groups. The levels of LDL-cholesterol in serum of the RN-HFC(-40, -400, -4000㎎/㎏) groups were tended to be lower than those of other groups. These results suggest that Rhynchosia nulubilis extract may reduce elevated levels of serum lipid concentrations in rats fed high fat diets.

여름에 비정형 폐렴으로 발현한 쯔쯔가무시병 1예

박상돈,정문현,이혜명,김미경,강재승 대한감염학회 2008 감염과 화학요법 Vol.40 No.4

Scrub typhus usually occurs in October and November in Korea. Its typical clinical manifestations are fever, rash, eschar, and lymphadenopathies. Pneumonitis may occur, however it occurs as a complication at the late phase of scrub typhus, when it is inappropriately treated. We encountered a case of pneumonitis that was an initial manifestation of scrub typhus which was confirmed by rickettsial culture, Positive seroconversion, and polymerase chain reaction. The case was a 16-year-old high school adolescent who presented with fever and headache that lasted for 1 day in July, 2007, He denied of any outdoor activities. A chest radiograph showed patchy infiltrates in the left lower lung, which improved with the administration of cefotaxime and azithromycin for empirical treatment of community-acquired pneumonia. Blood taken on the second febrile day showed a positive culture for Orientia tsutsugamushi and was also positive for O. tsutsugamushi DNA polymerase chain reaction. Immunofluorescent antibody (IFA) test for O. tsutsugamushi showed that the initial antibody was negative, but convalescent serum tested positive with an antibody titer of 1:80. IFA for Coxiella burnetii showed false positive results; the initial IgM and IgG liters were 1:128 and 1:256, respectively, and the IgM and IgG titers in convalescent serum were 1:128 and 1:128, respectively.

성별이 고객태도와 구매의도에 미치는 영향에 관한 연구 : 제품유형을 중심으로 Focus on Product Types

윤성욱,황경미 東亞大學校 經營問題硏究所 2003 經營論叢 Vol.24 No.-

식생활에서의 대추의 할용

권상호,조경열,김소연,김미정 대구효성가톨릭대학교 식품과학연구소 1993 식품과학지 Vol.5 No.-

식생활에서 대추의 이용성확대 방안을 살펴볼 목적으로 대추와 관련한 그간의 문헌들을 정리해 보았다. 그 결과 대추는 한방에서도 사용빈도가 가장높은 약재이면서 식품으로서도 가치성이 매우 커서 일상의 식생활에서 주식과 함께 이용함으로서 한방식생활의 보편화를 기하는데 가장 편리한 식품으로 평가되었다. 그러므로 대추를 식생활에 쉽게 이용할 수 있는 다양한 가공품의 개발과 응용방법의 확대방안 모색이 요망되었다. The review was summarized to investigate extensive possibilities of jujube fruit for the application of dietary life. Seventy seven referances were organized and discussed in this paper. Jujuba fruits have been used as the famous herb medicine and food materials in Korea, China, and Japan because they contain many kinds of nutrients and components which have a tonic action, urination action, and strength of physical recovery etc. In addition, it was estimated as the most desirable food materials for the dietary life together with the staple foods. Therefore, many studies for processing and application of jujuba fruits on the dietary life were required.

녹차 건분이 고지방식이 급여에 의한 흰쥐의 지질 농도에 미치는 영향

신미경,김덕희,한성희 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.3

The effects of dried green tea leaf powders on serum lipid concentrations were evaluated in rats. Sixty male Sprague-Dawley rats weighing 100±10g were divided into six groups and fed high fat diets for six weeks. Experimental groups were administered with following diets: Normal fat diet and normal and high fat diets with 1% dried green tea leaf powders. Tissue weights of live, lung, stomach, heart, kidney and spleen of high fat diet exposed rats were reduced by dried green tea leaf powders groups. The concentrations of serum triglyceride in rats fed the dried green tea leaf powers were lower than those in other groups. The concentrations of total cholesterol in green tea leaf powders the were lower powders green tea were significantly higher than those of other groups. The levels of LDL-cholesterol in serum of the dried green tea leaf powders groups were tended to be lower than those of other groups. GPT and GPT were decreased in dried green tea leaf powders groups and than in the high fat groups. LDHase was lower in the dried green tea leaf powders groups than in the high fat group. These results suggest that dried leaf powders green tea groups may reduce elevated levels of serum lipid concentrations in rats fed high fat diets.

THE INFLUENCE OF OBTURATION TIMING AND THICKNESS OF MINERAL TRIOXIDE AGGREAGATE ON SEALING ABILITY OF CANAL WITH OPEN APEX

곽경인,박동성,유현미,오태석 대한치과보존학회 2000 Restorative Dentistry & Endodontics Vol.25 No.4

임상에서 점차 Mineral Trioxide Aggregate (MTA) 에 대한 우수성이 소개되면서, apical matrix로의 사용은 주목할 만하다 할 수 있다. 본 연구의 목적은 개방형근관에서 MTA가 apical matrix로 사용될 때의 치근단 밀폐효과를 알아보고, 근관충전의 시기와 matrix의 두께가 치근단 밀폐에 미치는 영향을 치근단 미세누출의 측면과 matrix탈락빈도의 측면에서 관찰하고자 하는 것이다. 개방형 근관을 재현하고자 45개의 발거된 단근치에 #90크기로 근단공을 형성하였고, 투명레진등을 이용하여 치근단병소를 갖는 치조골을 재현한 후, 4개의 실험 군과 1개의 대조 군으로 분류하였다. A군: 2mm두께의 MTA matrix 형성후, 열연화된 Gutta-percha와AH26 sealer를 이용하여 즉시 근관충전. B군: 2mm두께의 MTA matrix 형성후, A군과 같은 방법으로 24시간 후 근관충전. C군: 4mm 두께의 MTA matrix 형성후, A군과 같은 방법으로 즉시 근관충전. D군: 4mm두께의 MTA matrix 형성후, A군과 같은 방법으로 24시간 후 근관충전. 대조군: matrix를 사용하지 않고 열연화된 Gutta-percha와 AH26 sealer를 이용하여 근관충전하였다. Matrix의 탈락이 있는 경우 기록하고 완전수세 후 다시 시행하였다. 색소침투정도의 평가를 위해 methylene blue 에 치아를 48시간 침수시킨 후 협설로 양분하였다. 각 시편들을 고배율 현미경 관찰 후 비디오촬영 하였고, digitalized image analysing program 을 이용하여 두명의 관찰자가 누출의 정도를 평가한 후, One-way ANOVA로 통계적 유의성을 검증하여 다음과 같은 결과를 얻었다. 1. Matrix를 사용한 실험 군이 matrix를 사용하지 않은 대조 군에 비해 유의성 있게 낮은 누출을 보였다(p<0.05) . 그러나 근관충전시기와 matrix의 두께를 달리한 실험군 내에서는 누출에 유의성 있는 차이가 나타나지 않았다. 2 모든 실험군에서 gutta-percha를 이용한 근관충전의 시기에 matrix탈락 및 gutta-percha 의 압출은 발생하지 않았다.

복발효 감식초의 품질

김미경,김미정,김소연,정대성,정용진,김순동 대구효성가톨릭대학교 식품과학연구소 1994 식품과학지 Vol.6 No.-

고품질의 감식초를 농가에서 자가생산하기 위한 기초적 자료를 마련할 목적으로 자연발효시킨 경우와 복발효시킨 경우의 품질을 비교하였다. 그 결과 복발효시킨 식초는 자연발효 식초에 비하여 초산농도가 1/2인 3%정도였다. 그리고 HPLC 법으로 측정한 유리당의 총함량은 자연발효 식초는 6%, 복발효시킨 것은 5%였다. 감식초내에 함유된 유기산류로는 oxalic acid, malic acid, lactic acid, citric acid, succinic acid, glutaric acid 등 이었으며 자연발효시킨 경우가 전반적으로 함량이 높았다. 특히 자연 발효시킨 경우 lactic acid함량이 높았다. 그러나 감과실의 flavour와 과실의 고유색상과 맛은 복발효시킨 경우가 강하게 나타났다. To prepare the basic information for the fermentation of high quality persimmon vinegar in the farm, the quality of the complex fermented persimmon vinegar(CFPV) was compared to that of naturally fermented persimmon vinegar(NFPV). The titratable acidity of the CFPV was 3% which was the half concentration of the NFPV. The content of total free sugar by HPLC was 6% in CFPV and NFPV was 5%. Organic acid compositions in persimmon vinegar were oxalic acid, malic acid, lactic acid, citric acid, succinic acid and glutaric acid in addition to acetic acid. Total content of the organic acids in NFPV was higer than that in CFPV. Especially, the content of latic acid in NFPV was very higher than that of CFPV. The flavour, color and taste of persimmon fruit itself in CFPV were stronger than those in NFPV.