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Nguyen, Mau Tuan,Lim, Jong Sung,Han, Myung Sae 한국잠사학회 1997 한국잠사곤충학회지 Vol.39 No.2
The microsporidian infection with Nosema, bombycis reconfirmed its high virulence and transovarial tranmissibility, However, the characteristic symptom of the spots like pepper grains on the diseased larval skin was no more recognized by present varieties of the silkworm. Transovarial transmission rate detected from moth was above 90% in dead eggs or dead larvae in the rearing by mulberry leaves, 80% in the newly hatched larvae starved to death. Transovarially transmitted N. bombycis was easily observed from dead eggs and larvae, and were suggested an individual inspection of a few of dead eggs for detection of the pathogenic spores. The progeny population provided indicative factors on the sampling of predictive and corrective inspection. The higher concentration of N. bombycis spores included in the hindabdormal part of infected moth, applicative on the simple method of individual moth inspection. For the predictive inspection of growned 5th-instar larvae, N. bombycix infection was detectable without microscopic observation by the unique symptom of turbid milky-white spots on the silk gland. Inspection of the meconia artificially discharged from silkworm moth, was also succesful of microscopic obsenation before crossing, without killing or homogenize the moths. The results provided a basis of rational methods for the inspection of N. bombycis infection of the silkworm.
Nguyen, Mau Tuan,Nam, Sung Hee,Park, Hyun Ro,Han, Myung Sae Korean Society of Sericultural Science 2004 International Journal of Industrial Entomology Vol.9 No.2
Six entomopathogenic fungus isolates, Beauveria bassiana J57A, Nomuraea rileyi J125A, Paecilomyces farinosus J3A, Paecilomyces fumosoroseus J50A, Metarhizium anisopliae J88, Aspergilius sp. J64A, causing muscardine disease and aspergillosis in the silkworm, Bombyx mori were investigated for their cultural and morphological characteristics (on PDA culture media within 14 days at $24^{\circ}C$). The results showed that they differ each other from the features of cultural characteristics (colony elevation, colony color, colony growth rate) or morphological characteristics (conidiogenous cell structure, phialides, conidia size and shape). Among cultural characteristics, colony color is the easiest recognizable character between isolates. The morphological characteristics of each fungal isolate correspond to the descriptions of current system of classification.
Han, Myung Sae,Mau Tuan Nguyen,Lim, Jong Sung 한국잠사학회 1997 한국잠사곤충학회지 Vol.39 No.2
Present experiment designed for the review of theoretical basis for the inspertion system of infected insects by Noema bombycis. A microsporidian N. bombycis, known as the high virulence, produced at the average of 7 X 108 spores per female moth of the silkworm, Bombyx mori, enabled transovarial tranmission. Detectability of N. bombycis spores in the mass inspection was varied by dillution level, the higher limit of dillution with healthy moths was 1:140 for 100% detection, 1:160 for 99.5%, 1:200 for 99.0%, For an efficient inspection under the microdcopic observation (600 X ), the lower limit of spore concentration was determined as 1,000,000 spores/ml, 60~80 moths could be applicable for a maximunl sample unit of a lot. Following the present inspection unit conditioned 35 to 40 moths for a lot. N. bombycis spores were easily detectable from the preparation of crude homogenate with 2% KOH, even the step of centrifuge was omitted. The results suggested a new basis of rational mass inspection system of silkworm female moths to save the facilities, labor, and time