Tight junction protein 1 is regulated by transforming growth factor-β and contributes to cell motility in NSCLC cells

( So Hee Lee ),( A Rome Paek ),( Kyung Sil Yoon ),( Seok Hyun Kim ),( Soo Young Lee ),( Hye Jin You ) 생화학분자생물학회(구 한국생화학분자생물학회) 2015 BMB Reports Vol.48 No.2

Tight junction protein 1 (TJP1), a component of tight junction, has been reported to play a role in protein networks as an adaptor protein, and TJP1 expression is altered during tumor development. Here, we found that TJP1 expression was increased at the RNA and protein levels in TGF-β-stimulated lung cancer cells, A549. SB431542, a type-I TGF-β receptor inhibitor, as well as SB203580, a p38 kinase inhibitor, significantly abrogated the effect of TGF-β on TJP1 expression. Diphenyleneiodonium, an NADPH oxidase inhibitor, also attenuated TJP1 expression in response to TGF-β in lung cancer cells. When TJP1 expression was reduced by shRNA lentiviral particles in A549 cells (A549-sh TJP1), wound healing was much lower than in cells infected with control viral particles. Taken together, these data suggest that TGF-β enhances TJP1 expression, which may play a role beyond structural support in tight junctions during cancer development. [BMB Reports 2015; 48(2): 115-120]

Clostridium difficile에 의한 설사의 예후인자

김준형,김희정,구남수,김영근,최준용,신소연,박윤선,김연아,김명수,정수진,최희경,송영구,이경원,김준명 대한감염학회 2007 감염과 화학요법 Vol.39 No.2

배경 : Clostridium difficile에 의한 설사(Clostridium difficile associated diarrhea; CDAD)는 임상 경과가 다양하다. 최근에 유럽과 북미에서 C. difficile 감염의 발생률이 증가하고 고전적 치료에 잘 반응하지 않으며 이환률이 증가하였고, 이러한 원인이 새로운 균주의 탄생에 기인한다고 보고되었다. CDAD의 예후에 영향을 미치는 세균성 요인과 숙주 요인을 확인하기 위해 본 연구를 진행하였다. 재료 및 방법 : 2002년 8월부터 2003년 12월까지 CDAD가 진단된 20세 이상인 환자들을 대상으로 후향적 Cohort 연구를 하였다. 세균성 요인을 확인하기 위해 cdt A, cdtB, tcd A, tcd A rep 그리고 tcd B 유전자(binary toxin)를 확인하였다. 설사가 치료 시작 후 11일 이상 지속되거나, 2달 이내에 재발하거나, 수술 혹은 다른 시술이 필요한 경우, 사망한 경우 예후가 좋지 않은 것으로 정의하였다. 결과 : 총 115예에서 toxin을 생성하는 C. difficile가 동정되었으며, Toxin A와 toxin B 모두 양성인 균이 91예, toxin B만 양성인 균이 24예였다. Toxin A 생성 여부가 예후에 영향을 미치지 않았지만, 제산제를 사용한 환자에서 그렇지 않은 환자에 비해 toxin B만 양성인 균이 많이 동정되었다(P<0.05). 예후가 좋지 않은 경우는 39예(33.9%)였고 76예(66.1%)에서 예후는 양호하였다. 단변량 분석에서 70세 이상의 고령, 남성, 증상 발현 후 사용한 항생제의 개수 사용, 증상 발현 후 carbapenem, aminoglycoside, glycopeptide 사용, 당뇨 및 뇌졸중 병력이 있는 경우, 그리고 높은 Charlson index가 불량한 예후 인자로 확인되었다. 그러나 독립적인 예후 인자를 조사했을 때에는70세 이상의 고령(odds ratio=3.378, P=0.009), 증상 발현후 carbapenem 사용(odds ratio 7.210, P<0.001)이 예후에 중요한 영향을 미치는 요소로 확인되었다. 결론 : 70세 이상의 고령과 증상 발현 후 carbapenem 사용이 CDAD 독립적인 불량한 예후인자이다. Background : Clostridium difficile associated diarrhea (CDAD) has a wide range of clinical manifestations. The prognostic factors of CDAD are not fully understood. Materialsand Methods : A retrospective cohort study of 115 patients with CDAD from Aug. 2002 to Dec. 2003 was conducted to evaluate prognostic factors of CDAD. Bacteriologic factors were determined by detecting the binary toxin gene, tcd A, tcd A rep and tcd B gene. Poor prognosis was defined as diarrhea more than 10 days even with classic treatment, recurrence, death, and moribund discharge. Results : Approximately 79% of isolated strains were toxin A+/B+ strains and 21% were toxin A-/B+ strains. There was no difference in prognosis between toxin A+ and toxin A- strains. 39 (33.9%) cases showed poor prognosis and 76 (66.1%) cases showed good prognosis. Univariate analyses revealed that the poor prognostic factors were old age over 70 years old, male, the number of antibiotics used after onset of symptom, the administration of carbapenems, aminoglycosides, glycopeptides after onset of symptom, history of DM and stroke, and high Charlson comorbidity index. Multiple logistic regression analysis identified old age over 70 years old (odds ratio=3.378, P=0.009) and the administration of carbapenems after onset of symptom (odds ratio 7.210, P<0.001) as the independent poor prognostic factors. Conclusion : Old age over 70 and the administration of carbapenems after onset of symptom were the poor prognostic factors for CDAD caused by none-binary toxin producing strains.

시ᆞ도 건강가정지원센터의 운영모형 개발 연구 : 역할 및 조직을 중심으로 It's Roles and Organization

장진경,오제은,한은주,류진아,원소연 한국가족관계학회 2006 한국가족관계학회지 Vol.11 No.1

간호실무에서 신봉이론(Espoused theories)에 대한 탐색적 연구

서문자,김혜숙,이은희,박영숙,조경숙,강현숙,임난영,김주현,이소우,조복희,이명하,지성애,하양숙,손영희,권성복,김희진,추진아 성인간호학회 2001 성인간호학회지 Vol.13 No.1

As a nursing practice involves nurses' actions in a specific context of health care, this study has focused on exploring the espoused theories in nursing practice within the action science perspectives, Espoused theories are the belief, principles, and rationale expressed by the practitioner as guiding her/his actions in a situation of practice. The data were analysed qualitatively and 25 elements of espoused theories of nursing action were identified and clustered into 6 categories. The 25 elements of espoused theories are as follows: The clinical nurse worked in wholistic and individual nursing, focussed on the patient's needed, comfort and supportive nursing (5 theories of nursing goal) ; excellent skills, knowledge based, assessment and data collection, explaining, educating or a scientific basis(6 theories of nursing intervention): advocacy, value oriented, treatment, account- ability and commitment(4 theories of nursing ethics) ; human respect. partnership, trust(3 theories of patient-nurse relationship) : knowledgable, accumulated clinical experiences and personally lived experiences. positive perspectives(4 theories of nurse), role of intervention. rewarding peer relationship(3 theories of situations) The above mentioned espoused theories are similar to that of nursing textbooks which students learned through basic nursing education and almost the same as the Acts ofa Nurse in Korean. However, we are doubtful whether nurses actually do as they think. Therefore. it is recommended to review the theories-in-use in order to and any discrepancies between the espoused theories and the reality of nursing actions

Synthesis and Antiproliferative Activity of Pyridinylcarbonylpyrimidines Against Melanoma Cell Line

Ahn, Hye-Mi,Lee, Jun-A,Kim, Hwan,Oh, Chang-Hyun,Lee, So-Ha,Sim, Tae-Bo,Hah, Jung-Mi,Kim, Dong-Jin,Yoo, Kyung-Ho Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.4

The synthesis of the series of pyrimidinylamines 1a-d and pyrimidinylureas 1e-u bearing a novel pyridinylcarbonylpyrimidine scaffold and their antiproliferative activities against A375 human melanoma cell line were described. Among them, three compounds 1e, 1h, and 1o showed superior antiproliferative activities to Sorafenib ($IC_{50}=5.5{\mu}M$) as a reference compound. In our series, urea compound 1o having 4-chloro-3-trifluoromethyl moiety on the benzene nucleus exhibited very good antiproliferative activity with $IC_{50}$ value of $1.4{\mu}M$.

Log-Rolling Block Copolymer Cylinders

Kim, Ye Chan,Kim, Dong Hyup,Joo, Se Hun,Kwon, Na Kyung,Shin, Tae Joo,Register, Richard A.,Kwak, Sang Kyu,Kim, So Youn American Chemical Society 2017 Macromolecules Vol.50 No.9

<P>Shear is an effective method to create long-range order in micro- or nanostructured soft materials. When simple shear flow is applied, particles or polymer microdomains tend to align in the shear direction to minimize viscous dissipation; thus, transverse alignment (so-called log-rolling) is not typically favored. This is the first study to report the transverse alignment of cylinder-forming coil coil block copolymers. Poly(styrene-b-methyl methacrylate), PS PMMA, where the PS blocks form the matrix, can adopt a metastable PMMA hemicylindrical structure when confined in a thin film, and this hemicylindrical structure can orient either along the shear direction or transverse to the shear direction depending on the shearing temperature. A monolayer of PS PMMA forming full cylinders exhibits log-rolling alignment. This unusual log-rolling behavior is explained by the low chain mobility of the cylinder-forming PMMA block at low temperatures, which is direction of shear alignment. the critical quantity determining the</P>

7학년 분자의 운동 단원에서 사회적 상호작용을 강조한 탐구실험 수업의 효과와 학생들의 인식

장진아,이경희,박소영,김동진,최병순,박국태 韓國敎員大學校 敎育硏究院 2009 敎員敎育 Vol.25 No.3

이 연구는 7학년 분자의 운동 단원에서 사회적 상호작용을 강조한 탐구실험 수업이 중학교 1학년 학생들의 개념 이해와 학업 성취도에 미치는 효과와 학생들의 인식을 알아보기 위한 것이다. 연구를 위하여 S시 소재 Y중학교 1학년 3개 학급 107명을 실험집단l파 실험집단2 및 통제집단으로 나누어, 실험집단l에는 학생간의 사회적 상호작용을 강조한 탐구실험 수엽을 실시하고, 실험집단2에는 교사와 학생간의 사회적 상호작용을 강조한 탐구실험 수업을 실시하였으며, 통제집단에는 전통적인 실험 수엽을 실시하였다. 수업 전과 후에 개념 이해도 검사를 하였으며, 성취도 검사와 인식 조사는 수업 후에 실시하였다. 연구 결과에 의하면, 실험집단1과 섣험집단2의 사회적 상호작용을 강조한 탐구싣험 수업이 통제집단의 전통적인 설험 수엽에 비하여 분자의 운동에 대한 개념 이해에 효과적이었다. 학업 성취도에 있어서는 사회적 상호작용을 강조한 실험집단1과 실험집단2의 학업 성취도 평균이 통제집단의 학업 성취도 평균에 비해 통계적으로 유의마하게 높았다. 실험집단1과 실험집단2의 학생들은 전반적으로 분자의 운동 단원에서 사회적 상호작용을 강조한 탐구실험 수업을 긍정적으로 인식하였다. 실험집단1에서 인지수준이 높은 학생들은 명확하게 답을 제시해 주는 것보다 동료와의 상호작용을 통해 시행착오를 거치면서 스스로의 힘으로 문제를 해결함으로써 자신감과 만족감을 느꼈다. 실험집단2에서 인지수준이 중하위인 학생들은 애매하고 시행착오적인 동료와의 상호작용보다는 교사와의 상호작용을 통해 직접적으로 답에 이르고 명확하게 개념 정리를 해주는 것을 선호하였다. The purpose of this study was to investigate effects of the inquiry experiment instruction emphasizing social interaction in the 7th grade motion of molecules unit on middle school students' scientific concept, achievement, and the perception of the instructions. For this study, 107 7th grade students were divided into three groups: the experimental group I, the experimental group 2, and the control group. The experimental group 1 were given inquiry experiment instruction emphasizing student-student interaction and the experimental group 2 were given inquiry experiment instruction emphasizing teacher-student interaction, while the control group were given traditional experiment instruction. They were all tested on their level of scientific concept understanding before and after the instruction and after the instruction they were given achievement test and surveyed on their perception. According to the result of this study, the inquiry experiment instruction emphasizing social interaction of experimental group 1 and 2 was more effective than the traditional experiment instruction of the control group in understanding the scientific concept of molecular motion, and the achievement level of experimental group 1 and 2 was significantly higher than the control group. Generally both students of group 1 and 2 were found to affirmatively perceive the inquiry experiment instruction emphasizing social interaction in the motion of molecules unit. Students of group 1 with high cognitive level were satisfied when they were able to independently solve problems by trial and error through student-student interaction than being given the exact answers. Meanwhile students of group 2 with middle to low cognitive level preferred the inquiry experiment instruction emphasizing teacher-student interaction that easily and cleary gives them the correct answer than student-student interaction which can be obscure and requires trial and error.

Oleifoliosides B-mediated autophagy and apoptosis involved Caspase Independent Pathways in A549 cells

Hai Yang Yu,Hyung-In Moon,Kyung-Mi Kim,Eunsook Chung,Selvam Ayarpadikannan,Hyun-A So,Jae-Sung Kwak,Kenneth Ryan Schraufnagle,Kim Hyo Young,Jai-Heon Lee(이재헌) 한국약용작물학회 2012 한국약용작물학회 학술대회논문집 Vol.2012 No.1

Mono-, Di-fluoro 5, 10, 15, 20-tetraphenylporphyrin Iron(Ⅲ)의 분광학적 연구

이인숙,신지영,김소영,김경아,김혜균 서울여자대학교 자연과학연구소 1997 자연과학연구논문집 Vol.8 No.-

Resonance Raman(RR) spectra of 5,10, 15, 20-tetraphenylporphyriniron(III) difluoride, [Fe(TPP)F_2], which was formed by the reaction between Fe(TPP)Cl and tetrabutylammonium fluoride(TBAF 3H_2O) in various non-aqueous solvents, were obtained with the 441.6nm(He-Cd laser) excitation. [Fe(TPP)F_2] is six-coordinate iron porphyrin with two polar Fe-F bonds. The porphyrin skeletal modes can be correlated with previously recorded mode of Ni(TPP) and [FeTPP]_2O. And these assignments were confirmed by observed wavenumber shifts due to ??Fe/^54Fe and TPP/TOO-d_8/TPP-N_15 isotopic substitutions. Difluoroiron complex is an iron(III) high-spin complex with the oxidation sensitive band at 1347cm^-1 for v_4 and core size/spin state sensitive band at 1541cm^-1 for v_2.

시판막장의 품질 특성 및 항산화성

전소현,신숙경,김현정,민아영,김미리,Jeon, So Hean,Shin, Suk Kyung,Kim, Hyun Jeong,Min, A Young,Kim, Mee Ree 한국식품조리과학회 2015 한국식품조리과학회지 Vol.31 No.1

The purpose of this research is to evaluate the quality characteristics and antioxidant activities of Makjang, Korean traditional fermented soybean paste, which has recently been disappearing, for its preservation. Six kinds of commercial Makjang from three different regions (Kang-won-do, Choong-chung-do, and Kyung-sang-do) were analysed for approximate composition, salinity, pH, total phenol contents, and DPPH and hydroxyl radical scavenging activities. Moisture content of samples was 48.30-58.93% while, crude protein was 9.42-13.67%. Crude fat was 2.45-6.50%, crude fiber was 2.08-6.45%, and ash was 6.59-14.64%. Salinity content ranged from 5.63-12.68%, and pH ranged from 4.36-5.67. Soluble solid content and reducing sugar content of samples ranged from 38.3-54.5 Brix and 22.38-31.61% respectively. The lightness, redness, and yellowness of the Hunter color system of samples were 16.58-28.19, 7.8-16.51, and 8.35-14.21, respectively. Total polyphenol contents were 0.20-0.45 mg/ml. Antioxidant activities determined by DPPH radical scavenging activity and hydroxyl radical scavenging activity ($IC_{50}$ value) ranged from 45.07 mg/ml to 95.93 mg/ml and 69.81 mg/ml to 309.40 mg/ml, respectively. From these results, it was suggested that the manufacturing process of Makjang is needed to standardize for quality control, and for mass production.