위암환자에서 BrdU에 의하여 유도되는 Fragile sites의 발현

윤숙자,한승로,정옥,김수일,박경란,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

The expression frequency of BrdU-induced fragile sites was examined in stomach cancer patients to determine whether this parameter could be used as a marker of genetic susceptibility in at-risk individuals. G banding performed to detect fragile sites. No difference was found in expression frequency between the stomach cancer patients and a group of normal individuals(p=0.7). This indicates that the expression frequency of BrdU-induced fragile sites is not a suitable marker for assessing genetic susceptibility in stomach cancer patient.

ATHB12, an ABA-lnducible Homeodomain-Leucine Zipper (HD-Zip) Protein of Arabidopsis, Negatively Regulates the Growth of the Inflorescence Stem by Decreasing the Expression of a Gibberellin 20-Oxidase Gene

Ora Son,허윤선,김윤경,이현정,김성한,김미란,남경희,이명석,김부용,박종범,박정안,이석찬,Atsushi Hanada,Shinjiro Yamaguchi,이인정,김승기,윤대진,Eva So¨derman,천충일 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

Cadmium Sulfate가 肝組織에 미치는 影響에 관한 形態學的 硏究

윤강호,박경란,김수일,노승무,김원식 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

Cadmium is well known toxic heavy metal which has been related with various pathologic conditions of liver, kindney, testis, cardiovascular system and central nervous system etc.. In this experiment, cadmium sulfate (1.2mg/100gm b.w.) was given intravenously into the mouse (Balb - C, b. w. 20 - 25gm) via tail vein for the study on morphological changes of liver. On the 8hours, 16hours and 24hours after administration, mice were sacrificed under ether anesthesia, liver was removed, then, a part of liver was fixed in 10% neutral buffered formalin solution, processed routinely, stained with hematoxylin & eosin, and observed under light microscope. Otherwise, the remainder portion of liver tissue was prefixed in 1.5% glutaraldehyde solution, postfixed in the 1% osmium tetraoxide solution, embedded with Epon 840, and observed under transmission electron microscope(H-7000, Hitachi Co.). The results obtained were as followings ; 1. On light microscopic observation, hemorrhagic necrosis at the zone Ⅰ and zone Ⅱ with congestion of hepatic lobules were found. 2. On transmission electron microscopic observation, cisternal dilation of RER & SER, autophagic vacuoles, myelin figures, and certain electron dense materials within the hepatocytes were found. Although this observations were limited in mice, the above findings of its acute effects on liver morphology will serve morphological basis for the better understanding of toxic effects on liver after chronic exposure or multiple interactions with other toxic substances.

ATHB12, an ABA-lnducible Homeodomain-Leucine Zipper (HD-Zip) Protein of Arabidopsis, Negatively Regulates the Growth of the Inflorescence Stem by Decreasing the Expression of a Gibberellin 20-Oxidase Gene

Ora Son,허윤선,김윤경,이현정,김성한,김미란,남경희,이명석,김부용,박종범,박정안,이석찬,Atsushi Hanada,Shinjiro Yamaguchi,이인정,김승기,윤대진,Eva So¨derman,천충일 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

치료저항 정신분열병 환자에서 Clozapine 사용이 삶의 질에 미치는 영향

김은경,서경란,윤창영 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.6

연구목적: 본 연구는 clozapine 치료가 치료저항 정신분열병 환자의 삶의 질에 미치는 영향을 관찰하는 전향적 개방 임상연구이다. 방 법: DSM-Ⅳ 진단기준에 의거하여 정신분열병으로 진단되고 기존의 항정신병 약물에 반응하지 않는 치료 저항 정신분열병 환자 23명을 대상으로 하였다. Clozapine을 투여하기 전에 기초평가를 하고 약물투여후 PANSS, BPRS, Simpson-Angus Rating Scale을 2주간격으로 12주동안 반복 시행하였고, 12주이후부터는 4주에 1회씩 반복 시행하였다. 삶의 질을 평가하기위해 Heinrichs 등이 고안한 삶의 질 척도(QLS)를 사용하여 매달 1회씩 반복 측정하였다. 결 과: 23명중 5개월의 clozapine 효과를 검증한 환자는 총 18명이었다. PANSS 양성, 음성 및 일반정신병리척도, BPRS점수는 기초평가에 비교하여 5개월 후에 통계적으로 유의한 감소를 보였다. 약물 부작용은 기초평가에 비하여 5개월 후에 통계적으로 유의한 변화는 보이지 않았다. 삶의 질은 5개월 후 평가에서 전체점수가 기초평가에 비하여 52.69% 상승하였고, QLS 전체점수와 4개의 하위척도중 대인 관계 영역과 내적인 정신요소 영역에서 기초평가에 비하여 5개월 후에 통계적으로 유의한 상승을 보였다. PANSS 음성척도 및 일반병리척도가 삶의 질과 유의한 관련성을 보였다. 결 론: Clozapine 치료는 기존의 치료에 반응하지 않는 치료저항 정신분열병 환자의 삶의 질에 긍정적인 효과를 가져왔다. Objectives: This prospective study was performed to determine effects of clozapine treatment on the quality of life of treatment-resistant schizophrenic patients. Method: Subjects were 18 patients with treatment-resistant schizophrenia. Patient`s PANSS, BPRS and Simpson-Angus Rating Scale was assessed at 9 time points: baseline, the 2th, 4th, 6th, 8th,10th, 12th, 16th, and 20th weeks of treatment. We chose to use the Quality of Life Scale(QLS) developed by Heinrichs and associates to evaluate the effect on the quality of life. The QLS was scaled at every month of treatment. Results: Eighteen of twenty-three patients completed the five months trial of clozapine. Three PANSS factors(positive, negative, general) and BPRS total scores showed a significant improvement at the fifth month of clozapine treatment. The adverse effects of clozapine did not show a significant improvement. After five months of clozapine treatment, there were an increase of 52.69% in the total QLS score and a significant improvement of two QLS factors(interpersonal relations, intrapsychic foundation). Particularly two PANSS factors(negative, general) had a significant correlation with the QLS score. Conclusions: This study suggests that clozapine treatment has a positive effect on quality of life of treatment-resistant schizophrenic patients.

Scenedesmus quadricauda var. biornatus Kiss의 형태변이

김미란,안선숙,장윤경 숙명여자대학교 자연과학연구소 1992 자연과학논문집 Vol.- No.3

6 strains of S. quadricauda var. biornatus Kiss which is new to Korea were isolated from 6 different localities in Seoul and Kyunggi-do area in order to examine their taxonomic identity. Morphological variations of the coenobia, percentage of coenobia formed and the ultrastructure of the cell wall were observed in both synchronized and stationary cultures. Three different coenobial types, i.e. abundans type, biornatus type and quadricauda type were produced in all isolates. The percentages of coenobial types produced in different strains varied depending on the culture period. The cell wall structure of all but one(st. QB-6) strain were identical and constant through out the culture period. The cell wall structure of S. quadricauda var biornatus were unique in its smooth surface and the presence of 1 or 2 rosette, and could be easely distinguished from those of S. quadricauda, S. armatus, S. ellipsoideus and S. intermedius which produce biornatus type coenobia during early log phase of growth.

Prednisolone과 Prednisone의 임상약동학적 평가

신재국,윤영란,차인준,이경훈,장인진,신상구,박찬웅 大韓臨床藥理學會 1994 臨床藥理學會誌 Vol.2 No.1

운동과 적혈구 노화와의 관계

최용어,윤탁영,홍경란 韓國體育大學校附屬 體育科學硏究所 1991 韓國體育大學校附屬 體育科學硏究所論文集 Vol.10 No.1

본 연구는 노화의 정도에 따라 적혈구를 분리하고 장시간의 운동 스트레스가 노화에 미치는 영향을 알아 보고자, 고도로 훈련된 남자 마라톤 선수 3명을 대상으로 HRmax 80% 수준에서 지속적인 런닝을 실시하였다. 전혈에서 RBC, WBC, MCV, Hb, Hct를 측정하고, 적혈구 내에 존재하는 Protein Carboxyl O-Methyltransferase(PCM)을 정제하여 그 activity를 측정하고, 적혈구 막을 분리하여 그 구성 단백질에 대한 PCM 의 Methylatability를 측정하여 운동과 적혈구 노화와의 관계를 설명하고자 한다. 각 시간별, Rest(0분), Running(30, 60, 90, 120분), Recovery(10, 30분)에 혈액을 채혈하여, 전혈에서 RBC, WBC, MCV, Hb, Hct의 변화를 조사하고 (Hematology Analyzer 5), 적혈구를 분리하여 50% Prcoll과 0.154M Hypaque에 넣고 22,000??g에서 40분간 원심분리하면 11 -15개 정도의 적혈구 층이 노화된 정도에 따라 분류된다. 적혈구 세포내의 PCM을 정제하고, 적혈구 막(ghost)을 분리하여 방사성 동위원소 (??C-SAM)를 methyl donor로 하여 PCM activity와 적혈구 막에 대한 Methylatability를 측정하였다. 런닝 30분에 RBC수가 증가하였으며, WBC와 Hb은 운동하는 동안 높은 수준을 유지하였다. MCV와 Hct는 변화가 없었다. Percoll과 Hypaque를 이용하여 노화된 정도에 따라 적혈구를 분류하였으나, 각 층에 대한 세포수의 측정이 뒤따르지 못하여 적혈구 수의 증가 혹은 감소와의 관계를 직접 볼수 없었다. 적혈구 내에서 정제된 PCM activity 는 운동 스트레스에 의한 변화를 보이지 않음으로써 항상 full을 유지하고 있음을 알 수 있다. 적혈구 막에 대한 Methlatability변화는 운동전 26.77±0.32 units/mg.min에서 런닝 30분에 34.29±0.55 units/mg.min로 큰 증가를 보였으며 런닝하는 동안 높은 수준을 유지하였다. 이는 운동 스트레스가 적혈구 막단백질을 구성하는 아미노산에 영향을 미쳐 비정상의 L-isoaspartyl 잔기를 만들었음을 말해주는 것이며, 이것이 노화된 단백질의 수복기전과 일치한다. 즉 PCM이 비정상의 L-isoaspartyl 잔기를 메칠화시켜 정상의 L-aspartyl잔기로 수복시킴으로써 운동 스트레스에 의한 영향을 극복하고자 하는 것이다. 그러므로 장시간의 운동 스트레스가 주는 영향이 노화에 의한 현상과 일치함을 알수 있다. Our studies showed the separation method of erythrocyte according to aging process and the effect that influenced a long-time exercise stress to aging. Highly trained marathon runners (n=3) performed a prolonged running(2hours) at the level of HRmax 80% RBC,WBC,MCV, Hct was measured in a whole blood.Protein Carboxyl O-Methyltransferase(PCM) in the erythrocyte was purified from the cytosol and the activity was measured. Methylatability of erythrocyte membrance protein was measured during marathon performance and recovery phase. Therefore, we interpreted the relation of exercise and erthrocyte aging. The count of RBC increased at the running 30 min. WBC and Hb maintained at the high level during marathon running. MCV and Hct did not changed. Erythrocyte divided into 11-15bands by Percoll and Hypaque according to aging. Because the erythrocyte did not count numerically in different age-related fraction of human erythrocyte, we cannot show the change of erythrocyte count. PCM activity purified in erythrocyte did not change by exercise stress, this suggests that PCM is full at all times. Methylatability of erythrocyte membrane protein changed from 26.77±0.32 units/mg.min before exercise to 34.29±0.55 units/mg.min at 30min after exercise and maintained a high level of methylatability during a running. This suggests that abnormal L-isoaspartyl residues came into being in the membrane protein composition as a result of exercise stress and are repaired by the same mechanism as aging repair that is, PCM methylated abnormal L-isoaspartyl residues in membrane protein and repaired into normal L-aspartyl residues, which removed the effect of exercise stress. Therefore, this study showed that the effect by exercise stress is the same as that of aging process.

Case Report : Carvenous lymphangioma of the vulva

( Kyung Ran Yoon ),( A Jin Mo ),( Sung Ho Park ),( Yeon Sik Na ),( Sung Taek Park ) 대한산부인과학회 2015 Obstetrics & Gynecology Science Vol.58 No.1

Lymphangioma is a rare proliferation of the lymphatic system which is classified as either lymphangioma circumscription or carvenous lymphangioma. The involvement of the vulva is very rare and only a small number of case reports have been made on carvenous lymphangioma of the vulva. We herein report a case of 20-year-old unmarried girl presented with gradually expanding and painless tumor of the left labium majus. The mass was removed surgically and pathology confirmed as carvenous lymphangioma, with no recurrences to date.

오가피 분획 추출물의 항산화효과

임경란(Kyung-ran Im),김미진(Mi-jin Kim),정택규(Teak-Kyu Jung),윤경섭(Kyung-Sup Yoon) 한국생물공학회 2008 KSBB Journal Vol.23 No.4