RBL-2H3 세포에서 IgE-dependent Histamine-releasing Factor의 탈인산화 효소에 관한 연구

황선옥,이경림 이화여자대학교 약학연구소 2005 藥學硏究論文集 Vol.- No.15

IgE-dependent histamine0releasing factor(HRF) was initially described as a secretagogue for secretion of histamine from IgE+basophils from a subset of allergic donors. Previously, we identified that S98 residue of HRF was phosphorylated using anti-HRFpS98 antibody which specifically recognizes the phosphorylated serine residue of HRF and HRFS98A mutant construct. In vitro kinase assay, only wild type HRF was phosphorylated by PKC, and S98A HRF was not affected by PKC. in this study, we attempted to characterize the phosphatase which specifically dephosphorylates HRF by immunoprecipitation and pull-down assay. In RBL-2H3 cells, HRF interacted only with calcineurin (also called as PP2B, calcium/calmodulin-dependent phosphatase ) but not with PP1 or PP2A. The results suggest that HRF is most likely dephosphory lated by calcineurin.

두가지 혈류 유발방법에 의한 새로운 심폐소생술(이중 혈류 유발 심폐소생술)장치의 개발

황성오,김현,조준휘,오범진,임종천,최경훈,윤정한,이승환,김영식,이강현,이윤선 大韓應急醫學會 1999 대한응급의학회지 Vol.10 No.2

Background: There have been many efforts to augment blood flow during cardiopulmonary resuscitation. These efforts have focused on maximizing the effect of cardiac pump or thoracic pump alone. However, considering that the heart is the biggest blood reservoir and increase of intrathoracic pressure can generate blood flow, simultaneous exploitation of both mechanisms may have synergistic effect. We hypothesized that simultaneous chest constriction in addition to sternal compression by standard CPR may have additive hemodynamic effects by preventing deformation of the chest and increase of intrathoracic pressure. Methods and results: we built a new mechanical device to perform compression and thoracic constriction simultaneously. The device consists of two main elements. Piston in the center is to depress the sternum. Strap is to constrict the thorax circumferentially. Strap is attached to both sides of the piston. When the piston is pushed down, it depresses the sternum and pulls on the thoracic strap. To determine strap width to produce optimal hemodynamic effect, we measured hemodynamic parameters with variable widths of strap in two dogs after induction of ventricular fibrillation. Result of the experiment showed that 10cm wide strap was determined to be most effective. We also determined optimal depth of compression to produce maximal hemodynamic effect with animal experiments using two dogs. Animal experiments showed that the highest aortic pressure could be generated when the stemum was depressed to 5 cm. Cardiopulmonary resusciation using a new device could generate higher systolic aortic pressure, coronary perfusion pressure and end-tidal carbon dioxide tension in comparison with standard cardiopulmonary resuscitation in a pilot animal study using two dogs. Conclusion: New cardiopulmonary resuscitation method using a mechanical device designed by us could perform sternal compression and simultaneous thoracic constriction, and generate better hemodynamic effects than standard cardiopulmonary resuscitation in pilot animal experiments.

심정지환자에서 epinephrine투여시 혈중 catecholamine의 변화

임경수,황성오,이부수,이강현,이진웅,임종천,김영식,김선만,김 현 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Background : Cardiac arrest is a potent stimulus for the release of endogenous catecholamine and high plasma catecholamine concentration has been reported during cardiopulmonary resuscitation(CPR). Few data were present about the clinical effect of high plasma catecholamine and endogenous catecholamine response to exogenous epinephrine administration during CPR. Purpose : This study was designed to evaluate endogenous catecholamine response to cardiac arrest and exogenously administered epinephrine during CPR. Method : In 21 cardiac arrest victims, blood sample for epinephrine and norepinephrine was drawn before and 1 minute after each epinephrine administration during CPR. 1mg of epinephrine was administered immediately after intravenous access and 3 minute interval during CPR. Plasma catecholamines were quantitated by high-performance liquid chromatography. Result : Baseline plasma epinephrine concentraqtion was higher in patients with cardiac arrest than normal controls (985±1627 vs 194±173 pg/ml, p<0.05). Baseline plasma norepinephrine concentration was also elevated in patients with cardiac arrest than normal controls, although statistically insignificnat(1965±4915 vs 360±250 pg/ml). Plasma epinephrine concentration in cardiac arrest patients was significantly elevated after exogenous administration of epinephrine, but its response was blunted after the third does of epinephrine. Plasma norepinephrine concentration in cardiac arrest patients was also elevated, but its response was observed only after the first epinephrine administration. Plasma epinephrine concentration was persistently higher in patients with return of spontaneous circulation (ROSC) than patients without ROSC. there was no significant difference of plasma norepinephrine concentration whether spontaneous circulation was restored or not. Endogenous epinephrine response to exogenous epinephrine was not observed in patients having prolonged arrest time over 20 minutes. Conclusion : Results of this study suggests that significant amount of catecholamine is released by exogenous epinephrine administration in spite of high plasma catecholamine concentration, and degree of endogenous catecholamine response influences resuscitation outcome.

12-O-Tetradecanoylphorbol-13-Acetate에 의한 HL-60 세포 분화유도중 Vimentin 유전자 전사조절에 대한 AP-1의 역할

임규,김진희,권도원,김승민,이명선,윤경아,손미영,박종일,윤완희,황병두 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

Purpose: To gain insight on the role of AP-1 in transcriptional regulation of vimentin gene during differentiation of HL-60 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA), the levels of vimentin mRNA and AP-1 have been investigated with Northern blot hybridization and DNA mobility shift assay. Materials and Methods: HL-60 cells were grown in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum and antibiotics in a humidified 5% CO_2 at 37℃. Total RNA was prepared by a modification of the method of Karlinsey et al. Northern blot hybridization was performed by the method of Virca et al. EcoRI fragment of pVIM-GEM was used as probe for vimentin mRNA. DNA mobility shift assay was performed by the method of Lim et al. End labeled DNA probe(Upper strand, 5'-CGCTTGATGAGTCAGCCG- 3') for AP-1 binding activity was mixed with nuclear extracts in a 20 μl reaction volume containing 300 mM KCI, 60 mM HEPES, pH 7.9, 25 mM MgCI_2, 1 mM EDTA, 1 mM DTT, 60% glycerol, and 2 ㎍ of poly[dI-dC]. Results: TPA increased vimentin mRNA levels, with maximal stimulation reached at 24 hr. The level of vimentin mRNA was induced in proportion to the concentration of TPA. TPA-induced vimentin mRNA was almost reduced by actinomycin-D pretreatment. TPA-induced stimulation of vimentin gene was completely reduced by staurosporin pretreatment. In DNA mobility shift assay, AP-1 newly appeared at 24 hr during TPA-induced differentiation and was almost not detected after the pretreatment of staurosporin. Conclusions: These results suggest that the induction of vimentin mRNA during TPA-dependent differentiation in HL-60 cells may be mediated by protein kinases C signal transduction and AP-1 is important to transcriptional regulation.

고정성 교정장치 장착 환자에서의 NaF 양치액 사용시 Cariescreen을 이용한 Streptococcus mutans 변화에 관한 연구

황충주,임선아,김경엽 대한치과교정학회 1998 대한치과교정학회지 Vol.28 No.1

부정교합의 치료를 위해 사용하는 고정식 교정장치는 치아 이동에는 매우 효율적이지만 치면 세균막 관리는 어렵게 된다. 이에 따라 치태 및 세균이 증가하고 결과적으론, 치아우식 및 치아표면의 탈회와 같은 부작용이 발생하게 된다. 본 연구에서는 고정식 교정장치로 치료하는 환자에게서, 탈회 및 치아우식을 낮추기 위해 0.05%의 NaF와 10% Xylitol이 함유된 양치액을 사용하여 시기별로 효과를 평가하고자 하였다. 고정성 교정장치로 치료하는 12~14세의 아동(평균연령 12.6세) 환자 30명을 대상으로 하여, 각 15명씩 실험군과 대조군으로 구분하였다. 실험군에선 0.05%의 NaF와 10% Xylitol이 함유된 양치액을 사용하게 하였으며, 대조군에선 증류수를 주로한 placebo 양치액을 사용하게 하였다. 양치액 사용전과 사용후 2주, 4주, 6주, 8주시 Cariescreen SM kit를 이용하여 타액내 S. mutans의 수의 변화를 관찰하여 다음과 같은 결과를 얻었다. 1. 실험군에서 타액내 S. mutans의 수는 양치액 사용전과 2주(p<0.01), 4주(p<0.05), 6주(p<0.001), 8주(p<0.001)에서 또한 2주와 6주(p<0.05), 8주(p<0.05)에선 통계적 유의치가 나타났고, 반면에 대조군에선 각 측정시기간에 통계적 유의차는 보이지 않았다. 2. 각 측정시기간의 상관관계는 대조군에선 양치액 사용전과 2주, 4주, 6주, 8주 즉 매번 검사시마다 그 이전과 이후에 S. mutans의 수에 통계적으로 유의성있는 순상관관계를 나타내었다. 3. 실험군에선 양치액 사용전과 2주 사이에서는 상관관계를 보이지 않았으며 그후 2주, 4주, 6주, 8주에서 순상관관계를 보였다. The purpose of this study was to evaluate the effectiveness of gargling solution with 0.05% NaF and 10% Xylitol in orthodontic patients with fixed appliance. The sample consisted of 30 adolescent patients who were classified into an experimental group and a control group, 15 patients each. Experimental group was used experimental gargling solution and the control group was used with placebo solution. The change of S. mutans in saliva was observed by Cariescreen SM kit at pre and post 2, 4, 6, 8 weeks. The results were as follows. 1. There were significant reduction in the number of S. mutans in saliva between pre and post 2 weeks(p<0.01), 4 weeks(p<0.05), 6 weeks(p<0.001), 8 weeks(p<0.001) in experimental group. And significant reduction also were observed in the number of S. mutans in saliva between post 2 weeks, 6 weeks(p<0.05), and 8 weeks(p<0.05), but no significant reduction were showed in control group. 2. There were significant correlation in the number of S. mutans between each measurement time(pre and post 2,4,6,8 weeks)in control group. 3. There were no correlation between pre and post 2 weeks, but significant correlation were observed between 2,4,6,8 weeks in experimental group.

12-O-Tetradecanoylphorbol-13-Acetate에 의한 HL-60 세포 분화유도중 Vimentin 유전자 전사조절에 대한 AP-1의 역할

임규,김진희,권도원,김승민,이명선,윤경아,손미영,박종일,윤완희,황병두 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Purpose: To gain insight on the role of AP-1 in transcriptional regulation of vimentin gene during differentiation of HL-60 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA), the levels of vimentin mRNA and AP-1 have been investigated with Northern blot hybridization and DNA mobility shift assay. Materials and Methods: HL-60 cells were grown in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum and antibiotics in a humidified 5% CO_(2) at 37°C. Total RNA was prepared by a modification of the method of Karlinsey et al. Northern blot hybridization was performed by the method of Virca et al. EcoRI fragment of pVIM-GEM was used as probe for vimentin mRNA. DNA mobility shift assay was performed by the method of Lim et al. End labeled DNA probe(Upper strand, 5'-CGCTTGATGAGTCAGCCG- 3') for AP-1 binding activity was mixed with nuclear extracts in a 20 μl reaction volume containing 300 mM KC1, 60 mM HEPES, pH 7.9, 25 mM MgCl_(2), 1 mM EDTA, 1 mM DTT, 60% glycerol, and 2μg of poly[dI-dC]. Results: TPA increased vimentin mRNA levels, with maximal stimulation reached at 24 hr. The level of vimentin mRNA was induced in proportion to the concentration of TPA. TPA-induced vimentin mRNA was almost reduced by actinomycin-D pretreatment. TPA-induced stimulation of vimentin gene was completely reduced by staurosporin pretreatment. In DNA mobility shift assay, AP-1 newly appeared at 24 hr during TPA-induced differentiation and was almost not detected after the pretreatment of staurosporin. Conclusions: These results suggest that the induction of vimentin mRNA during TPA-dependent differentiation in HL-60 cells may be mediated by protein kinases C signal transduction and AP-1 is important to transcriptional regulation.

PC 기반의 플랫폼간의 상호 연동을 위한 Intelligent Mirroring

임경은,황대준 成均館大學校 科學技術硏究所 1995 論文集 Vol.46 No.1

Creating collaborative environment on heterogeneous PC-based multimedia platforms running on different operationg systems has been of great importance to allow natural interaction among remote users through interoperability. Intelligent Mirroring is incorporated into our Multimedia Distance Learning System for IBM PCs and Apple's Power Macintoshs running Windows (ver. 3.1, ver. 95) and System (ver. 7.1, ver. 7.5), respectively. Upon messages from platforms via Ethernet involved the distance learning situation, Intelligent Mirroring its functions based on system homogeniety: liasion of messages for homogeneous platforms, interpretation of events and conversion of data encoding formats for heterogeneous platforms involved in the interaction. In such a distance learning situation requiring high degree of interaction Intelligent Mirroring contributes not only to establishing function and content sharing at a fine grain level with low cost, but also to minimize traffics related to interpretation of events to be shared by platforms participating in the collaboration.

열성화상으로 인한 상후두부 통증에서 상후두신경차단의 진통효과 : 증례보고

임경준,이재황,고우석 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.3

Thermal bum of pharynx and larynx can cause dysphagia and dyspnea which can be life threatening. Pain of larynx due to thermal bum does not subside sufficiently to drug therapy, Physiologic therapy. Here a case of acute epiglottitis with severe pain, which was successfully treated with superior laryngeal nerve block, is reported.

고강도철근콘크리트 기둥의 변형능력에 관한 실험적 연구

황선경,임경택,윤석천 忠南大學校 産業技術硏究所 1997 산업기술연구논문집 Vol.12 No.2

Seismic design of reinforced concrete structures if based on the ductility approach that study to improve of strength and ductility in concrete members to require. This paper is, therefore, to study on the strength and the ductility of reinforced concrete columns subjected to high axial compression loads(0.35fc' .Ag). The test results from 1/3 scale models using high-strength reinforced concrete (fc'=560/㎠) is presented. For the research the experimental variables used the shape of shear reinforcing bar, the space of shear reinforced bar and axial force ratio. As a result, deformation capacity of reinforced concrete columns subjected to high axial compression loads affected in the influences of axial force ratio and the space of shear reinforced bar, the shape of shear reinforcing bar.

Sertoli 세포의 생리기능 및 호르몬에 의한 조절

임규,박청,윤경아,윤은진,박종일,박승길,황병두 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.2