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이건화 ( Gun Wha Lee ),김동균 ( Dong Kyun Kim ),나현식 ( Hyun Sik Na ),배준용 ( Jun Yong Bae ),최준호 ( Jun Ho Choi ),김양기 ( Yang Ki Kim ),김기업 ( Ki Up Kim ),어수택 ( Soo Taek Uh ),김동훈 ( Dong Hoon Kim ),황정화 ( Jung Hwa Hw 대한결핵 및 호흡기학회 2005 Tuberculosis and Respiratory Diseases Vol.58 No.1
Pulmonary sequestration is a very rare congenital malformation in which a mass of pulmonary tissue is detached from the normal lung and receives its blood supply from a systemic artery. It may be clinically asymptomatic or it has a wide spectrum of variou
기체상 유해화학물질 제거를 위한 이동형 와류식 세정장치 개발 및 가스 제거효율 분석
곽지현 ( Ji Hyun Kwak ),황승율 ( Seung Ryul Hwang ),이연희 ( Yeon Hee Lee ),김재영 ( Jae Young Kim ),송기봉 ( Ki Bong Song ),김균 ( Kyun Kim ),강재은 ( Jae Eun Kang ),이상재 ( Sang Jae Lee ),전준호 ( Jun Ho Jeon ),이진환 ( Jin Hw 한국환경농학회 2015 한국환경농학회지 Vol.34 No.2
BACKGROUND: In recent years, several researchers have focused on odour control methods to remove the harmful chemicals from chemical accidents and incidents. The present work deals with the system development of the hazardous. METHODS AND RESULTS: For on-site removal of hazardous gaseous materials from chemical accidents, mobile vortex wet scrubber was designed with water vortex process to absorb the gas into the water. The efficiency of the mobile vortex wet scrubber was evaluated using water spray and 25% ammonia solution. The inlet air velocity (gas flow rate) was according to the damper angle installed within the hood and with increase of gas flow rate, consequently the absorption efficiency was markedly decreased. In particular, when 25% ammonia solution was exposed to the hood inlet for 30 min, the water pH within the scrubber was changed from 7 to 12. Interestingly, although the removal efficiency of ammonia gas exhibited approximately 80% for 5 min, its efficiency in 10 min showed the greatest decrease with 18%. Therefore, our results suggest that the ammonia gas may be absorbed with the driving force of scrubbing water in water vortex process of this scrubber. CONCLUSION: When chemical accidents are occurred, the designed compact scrubber may be utilized as effective tool regarding removal of ammonia gas and other volatile organic compounds in the scene of an accident.
Direct Detection of Drug-Resistant Hepatitis B Virus in Serum Using a Dendron-Modified Microarray
( Doo Hyun Kim ),( Hong Seok Kang ),( Seong-suk Hur ),( Seobo Sim ),( Sung Hyun Ahn ),( Yong Kwang Park ),( Eun-sook Park ),( Ah Ram Lee ),( Soree Park ),( So Young Kwon ),( Jeong-hoon Lee ),( Kyun-hw 대한간학회 2018 Gut and Liver Vol.12 No.3
Background/Aims: Direct sequencing is the gold standard for the detection of drug-resistance mutations in hepatitis B virus (HBV); however, this procedure is time-consuming, labor-intensive, and difficult to adapt to high-throughput screening. In this study, we aimed to develop a dendron-modified DNA microarray for the detection of genotypic resistance mutations and evaluate its efficiency. Methods: The specificity, sensitivity, and selectivity of dendron-modified slides for the detection of representative drug-resistance mutations were evaluated and compared to those of conventional slides. The diagnostic accuracy was validated using sera obtained from 13 patients who developed viral breakthrough during lamivudine, adefovir, or entecavir therapy and compared with the accuracy of restriction fragment mass polymorphism and direct sequencing data. Results: The dendron-modified slides significantly outperformed the conventional microarray slides and were able to detect HBV DNA at a very low level (1 copy/μL). Notably, HBV mutants could be detected in the chronic hepatitis B patient sera without virus purification. The validation of our data revealed that this technique is fully compatible with sequencing data of drug-resistant HBV. Conclusions: We developed a novel diagnostic technique for the simultaneous detection of several drug-resistance mutations using a dendron-modified DNA microarray. This technique can be directly applied to sera from chronic hepatitis B patients who show resistance to several nucleos(t)ide analogues. (Gut Liver 2018;12:331-341)