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PCR을 이용한 느타리버섯 재배사 물로부터 세균성갈색무늬병 병원균 Pseudomonas tolaasii 검출
정규식 외 한국버섯학회 2003 한국버섯학회지 Vol.1 No.1
느타리 세균성갈색무늬병 병원균 P. tolaasii의 전염원을 파악하기 위하여 느타리 재배사에서 사용하고 있는 물로부터 PCR방법을 이용하여 병원세균을 검출한 결과, 주로 충북지방의 느타리 재배사에서 수집된 57개 물 시료 전체의 28.1%인 16개 물에는 ㎖당 1,000 cfu 이하의 일반 세균을 포함하고 있었으며, 54.4%인 31개의 시료가 1,001-10,000 cfu, 10.5%인 6개의 시료가 10,001-100,000 cfu, 그리고 7%인 4개의 시료가 100,001 cfu 이상의 세균을 포함하고 있었다. P. tolaasii의 경우 nested-PCR로 검출하였을 경우 전체의 5.3%인 3곳의 물이 Immunocapture-nested-PCR로 검출하였을 경우 전체의 35.1%인 20개의 물 시료로부터 P. telaasii 특이적 DNA가 증폭되었다. 물에 포함된 일반세균의 농도와 P. tolaasii 검출과는 상관없었다. 이상의 결과는 물로부터 병원균을 검출하는 방법으로 IC-nested-PCR 방법이 더 민감하고 우수한 방법이며, 여러 곳의 느타리 재배사에서 사용하고 있는 물이 병원균 P. telaasii으로 오염되어 있는 것을 제시하고 있다. Pseudomonas tolaasii causing brown blotch disease was detected by PCR from water samples collected from the oyster mushroom cultivation farms to find the contamination level of the pathogen in water. Sixteen water samples (28.1%) contain less than 1,000 cfu, 31 samples (54.4%) contain 1,001-10,000 cfu, 6 samples (10.5%) contain 10,001-100,000 cfu, and 4 samples (7%) contain of bacteria per milliliter. P. tolaasii-specific DNA band was amplified in 3 samples (5.3%) by nested-PCR and in 20 samples (35.1%) by immunocapture (IC)-nested PCR respectively. These results suggest that IC-nested-PCR was much more sensitive than nested-PCR in detection of P. tolaasii and a quite few waters using for oyster mushroom cultivation were contaminated with P. tolaasii.
누설전류 파형 측정에 의한 실리콘 고무의 표면 열화 평가
김정호,정규원,서광석,유영식,박용관 成均館大學校 科學技術硏究所 1999 論文集 Vol.50 No.1
In this study, we investigated the erosion resistance of the silicone rubber by measuring leakage current with Inclined-Plane Method. From the results of our study, it was observed that the typical leakage current waveform was the mixed figure of sinusoidal wave and rectifying wave. And, it is considered that the former is due to conductivity of the contaminant when the surface is wet, and the latter is due to the arc discharge of dry band. And, as the surface erosion is being accelerated, the proportion of the rectifying wave is increasing. And, the ratio of discharge duration to discharge-extinguish time changes and can be divided into three regions, which is similar to the changes of erosion depth and width. Therefore, it can be known that the erosion process has some correlation with the change of leakage current.
黃鍾奎,梁熙天,崔定植,權涌周 全北大學校 1977 論文集 Vol.19 No.-
New varieties of sweet potato Sinmi and Hwangmi were cultivated in the reclaimed hilly area of four kinds of soil series in Jeonbug province and their growth conditions such as cutting time, row spacing, application of fertilizers and plant growth regulators and covering effect were investigated. Besides these studies on cultivation of sweet potatoes, experiments on the change of chemical components of sweet potato tubers, adaptibility for storage and drying of sliced sweet potato tubers were carried out. The results are summarized as follows : 1. The early cutting of May 20, alley of 90 X 20cm, application of potassium(28kg/10a)and covering produced the high yield of sweet potatoes. The apllication of OED green did not increase the yield of sweet potatoes, 2. The yield of Hwangmi was higher than that of Sinmi. These two varieties of sweet potatoes grew well in all the test soil series except Bansan. By early cutting of May 20, the highest yield of Hwangmi was obtained in Songjung series soil and the highest yield of Sinmi was in Yesan. 3. Starch content was higher in Sinmi than in Hwangmi while yield of starch per unit area was high in Hwangmi because of its high yield of sweet potato tubers. Both Hwangmi and Sinmi showed very high water content of more than 70 per cent. The highest yield of total solid matter from Sinmi was on September 15 and that from Hwangmi on September 30. Sinmi and Hwangmi harvested on September 30 showed the highest starch content. 4. Variation in water content, starch, soluble sugar, protein and fat content during storage was minimum in the sweet potatoes stored in the temperature controlled storage chamber and was maximum in those stored in the cave under ground. As a result, percent of grade A sweet potatoes remaining was highest in the temperature controlled storage chamber and lowest in the cave under ground. 5. Hwangmi was more susceptible to Rhizopus nigricans than Sinmmi, especially under the humid condition. 6. Shape and thickness of slice did not affect the yield of dried sweet potatoes but affected the time needed for drying. Depending on the thickness and the shape of slice, variations of 36 hours and 12 hours in drying time were observed respectively. Drying table was most effective in drying of sweet potatoes and drying rack was also effective and required 2 days less time than mat. Dipping in water did not show any effect on drying time but improved the color of dried sweet potatoes.
김정념,이숭환,김정식,김진호,배윤오,박성규,윤상정,한현영,이헌영 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1
Omental infarction, the end result of impaired perfusion to the greater omentum, is a rare benign self-limiting clinical entity. The main clinical symptom is non-specific localized abdominal pain with a moderately raised white blood cell and erythrocyte sedimentation rate. These findings often mimic an abdominal surgical emergency. This condition is often misdiagnosed as acute appendicitis or cholecystitis. The characteristic feature of CT scan and ultrasonography provide non-invasive diagnosis in most patients with omental infarction. We report a case of patient whose CT scan showed the characteristic finding of omental infarction. The patient was improved spontaneously only with conservative care.
Ion Beam을 이용한 Ni-Co-Cu Alloy Thin Film의 Dry Etching에 관한 연구
안정식,장문식,이규용 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1
Much effort has been made worldwide in dry etching of new thin film by IBE, IRE, ECR etc. In this study, dry etching of Ni-Co-Cu alloy thin film grown by IBD on a glass substrate was performed using Ar^(+) or N_(2)^(+) ions of 2KeV as a function of ion dose by high current DuoPigatron type ion source. The experimental results showed that surface morphology of the Ar^(+) beam etched PR was rough at 2KeV energy, but the N_(2)^(+) beam etched was smoother. It could be surmised that an increase in the atomic radius was correlated to an increase in the energy of the bombarding ions, which leaded to damaged PR layer due to the thermal phenomena, such as thermal spike, cascade, etc. in PR surface. As the result, pattern were destroyed partially with the melted and flowed over area. Dry etching of the thin film, in turn, was more successful in N_(2)^(+) beam etching than in Ar^(+) beam and it seemed that N_(2) ion was more effective in removing the currently used PR. Observed results from SEM showed that the resolution was increased with increasing N_(2) ion dose up to 3.6X10^(19) ions/cm^(2) at 2KeV, beyond this proper ion dose the resolution was decreased and leaded the damaged pattern.
일측폐환기 시 Propofol, Ketamine 및 Enflurane이 동맥혈 산소 분압과 이산화탄소 분압에 미치는 영향의 비교
우남식,이규창,김민정,김윤수 건국대학교 의과학연구소 1999 건국의과학학술지 Vol.9 No.-
The mechanism which normally affects distribution of blood flow through unventilated areas of the lung is hypoxic pulmonary vasoconstriction, this acts to divert the blood to well ventilated alveoli, resulting in a better retio of ventilation to perfusion. Several reports have focused attention to the reduction or abolition of this reflex in the unventilated lung by most volatile anesthetic agents used in clinical practice. This response was not abolished by the intravenous anesthetic agents. 30 patients undergoing elective thoracic surgery were stuided to evaluate the effect of a propofol(n=10), ketamine(n=10) and enflurane(m=10) on the changes in PaO₂ and in PaCO₂ as reflections of shunt and ventilation respectively during one lung anesthesia. We have demonstrated that in all cases a PaO₂ in excess of 105.3mmHg, 79.5mmHg, and 177mmHg were achived with propofol, ketamine and enflurane. In all cases a PaCO₂ was under the 36.8mmHg. We concluded that both propofol and enflurane were satisfactory anesthetics for one-lung anesthesia. and ketanine was not ideal for one-lung anesthesia in adult patients.
소규호,김영수,홍재식,정준영,조재민 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.4
미곡의 장기저장에 따른 이화학적 특성의 변화를 조사하여 효율적인 저장방법을 구명하기 위한 기초적 자료를 제공하기 위해 4년간 조곡상태의 미곡 50가마니를 상온 창고에서 저장하면서 성분 변화를 조사하였다. 고내 온도의 변화는 계절에 따라 35℃ 정도였다. 미곡의 수분함량은 저장기간 동안 15% 이상으로 증가한 적이 없어 비교적 안전하였으나 곡온이 18℃이상 유지되는 매년 6월부터 9월까지의 4개월 동안은 고내 습도의 상승과 함께 미질 저하를 일으킬 수 있는 저장 위험 기간이었다. 저장기간 중의 환원당, amylose 함량, 조단백의 변화를 조사한 결과 각각 입고시 0.24%, 19.23%, 7.02%에서 4년간 저장후 각각 0.5%, 20.31%, 7.46%로 증가하는 경향이었다. 저장미의 amylogram 특성을 조사한 결과 저장 기간이 길어짐에 따라 최고 점도, 최종 점도 및 setback은 입고시 각각 449B.U, 610B.U, 161B.U에서 4년 후 493B.U, 715B.U, 222B.U로 높아졌다. 반면에 Breakdown은 입고시 125B.U에서 4년 후 76B.U로 낮아졌다. 저장미의 호화응집성(gel consistency)은 입고시 44.7mm에서 4년후 39.9mm로 감소하였으며 지방산도는 입고시 4.5KOH㎎/100g에서 4년 저장후 각각 24.4KOH㎎/100g로 증가하였다. 배아율과 배아활성은 각각 97%, 100%에서 4년 후 각각 0%, 0.4%로 감소하였다. 한편 미곡에 착생한 미생물상의 변화를 조사한 결과 저장기간이 길어짐에 따라 세균 착생립수는 감소하고 곰팡이의 착생립수는 다소 증가하는 경향이었다. In order to investigate the changes of rice qualities during 4 years storage of paddy stored in warehouse of normal temperature condition. Temperature in warehouse was changed more than 30℃ under the influence of average temperature outside of a warehouse. Water content of paddy was not increased over 15%. But, as average temperature in wasehouse was gone up 18℃ from June to September every years, it was supposed that these periods were to be deterioration of rice quality. On investigation of change in paddy components during the long term storage, reducing sugar, amylose, crude protein were increased 0.24%, 19.23%, 7.02% at enterance time to 0.5%, 20.31%, 7.46% 4 years later, respectively. Max. viscosity, final visocity and set back value by amylograph were increased 449B.U, 610B.U, 161B.U to 493B.U, 715B.U, 222B.U but breakdown was decreased 125B.U to 76B.U with the increase of storage period. Gel consistency of rice stored was decreased 44.7mm at enterance time to 39.9mm 4 years later. Fatty acid was increased remarkably 4.5KOH㎎/100g to 24.4KOH㎎/100g. Germination ratio and germ activity of paddy during long-term storage were decreased 97%, 100% to 0%, 0.4%, respectively. With the increase of storage period, contaminated paddy by molds increased and its by bacteria decreased.
인공 산성비 용액 함침 및 경사평면법에 의한 옥외용 실리콘 고무의 열화 특성 평가
김정호,정규원,유영식,양계준,박용관 成均館大學校 科學技術硏究所 1999 論文集 Vol.50 No.1
We investigated the effect of acid rain on the surface aging, and resistance to tracking and erosion of silicone rubber materials : First, we measured the contact angle, SEM, EDX, and weight reduction to investigate the surface aging caused by immersion in the artificial acid rain solution. From the results of a series of experiments, we concluded that the acid rain attacked the base polymer of the surface, and lead to the depolymerization. Second, we adopted Inclined-Plane Method to measure the resistance to tracking and erosion of silicone rubber, and rather not observably affected the immersed specimen in the tracking resistance than in the erosion resistance.
하천 횡단 지하구조물 시공 시 발생하는 오차원인 및 보완대책에 관한 연구
박춘식,장정욱,박진규 국립7개대학공동논문집간행위원회 2005 공업기술연구 Vol.5 No.-
This thesis studied construction errors and complement methods of steel pile pressure injection method. We studied Daesan, Changwon, Gyoungnam. We analyzed the construction results in terms of FEM analysis. The conclusions of the study are as follows. 1) We found that the settlement of steel piles was caused by a great disturbance of the underground, which had in turn been caused by the pressure injection of steel piles. The settlement of steel piles exceeded the allowable settlement. The following summarizes the results of Terzaghi's consolidation theory and FEM regarding ground reinforcement. 2) The Semi-Shield method and Micro Tunnelling method turned out to be the most appropriate method.
장정욱,박춘식,박진규 국립7개대학공동논문집간행위원회 2003 공업기술연구 Vol.3 No.-
This study examined geological characteristics of the clay in the area around Kaduck-do, Kangseo-gu, Pusan, to figure out the characteristic fundamentals of the clay. The results are shown in the following. (1) The physical and chemical characteristics are summarized in he following table. There were no meaningful changes according to the depth degree. ◁ 원문 표 참조 ▷ (2) The geological characteristics 1) Sedimetological and geochemical characteristics showed that the clay layer was formed under oceanic circumstances. 2) The mineralogy mainly includes clay materials such as quartz, feldspar, illite, chlorite, and kaolinite. 3) The results of radiocarbon dating by AMS showed 3,692±50 yr.B.P for the upper samples and 16,650±200 yr.B.P. for the lower samples.