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RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향
박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-
TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.
( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.
空氣流動中의 噴射가스 燃料의 擴散에 關한 實驗的 硏究 (Ⅰ)
朴涇錫,金錫魯 慶熙大學校 1989 論文集 Vol.18 No.-
The mixture formation process has a great, influence on the performance and exhaust gas characteristics in the direct injection engines, such as Hydrogen engines and Diesel engines. This paper presents a fundamental study on the mixing process of compound jets. In this experimental study, the tracers used are CO_2, H_2 and He. The average-velocity of the air injected is 3m/sec, 5m/sec, 8m/sec, and 10m/sec. To observe spreading behavior of gas fuel, the Schlieren apparatus was used to take a picture of the fluid density. Then the values of the potential core length(Z_P), the potential core length and the transient length (Z_t), and the visual extension angle (φ) were obtained by visualized photo-analysed. The visual extension angle is decreased irrelatively for variety of injected gas, and is increased with constant grade in the area transient jet is occured. Experimental parameters are the average injection velocity of the tracer and the average injection velocity of the air.
먹는 물에서의 비소 검출에 관한 연구 : ICP-USN법과 ICP-VGA법의 비교 Comparison ICP-USN method ICP-VGA method
박경선,김형석,조인성 慶熙大學校 地球環境硏究所 1999 지구환경논문집 Vol.10 No.-
Abstract The analysis of arsenic in drinking water with ICP-USN method and ICP-VGA method was studied. In USN method, wavelength of 193.696nm was used and in VGA method, 228.812nm was used. The values of arsenic of same sample in each method, MDL, RPD, recovery, accuracy and precision were studied. The values of 14 sample of drinking water were within 0.05ppm or were not detected. But in one sample high value of 0.044ppm was detected. MDL of USN was 0.013ppm and MDL of VGA was 0.0004ppm. This results showed lower MDL of VGA but RPD of USN was better than VGA. Recovery of two methods was same, but accuracy and precision of USN were better than VGA. Therefore ICP-VGA method could be used according to this result(MDL) when arsenic trace element detection in drinking water should be achieved.
朴涇錫,崔鍾宇 慶熙大學校 1986 論文集 Vol.15 No.-
This paper presents a fundamental study on the mixing process of the premixed mixture. Nonuniformity of fuel and air mixture influences the combustion characteristics and exhaust emissions of the premixed type combustor. The object of this study is to express the mixture uniformity statistically by means of measurement of the fluctuating concentration field with the high response hot wire concentration probe and to visualize the premixed mixture formation by smoke wire methods.
박경서,문운기,홍영표,최신석 忠南大學校 環境問題硏究所 2001 環境硏究 Vol.19 No.-
남한강 상류 수계, 박대천 일대를 대상으로 2001년 4월부터 9월까지 총 11개 지점을 대상으로 조사하였으며, 종조성, 어류상과 군집 구조를 분석하였다. 총 7과 25속 33종으로 1570개체가 조사 기간동안 확인되었다. 전체 어류군집 중, 잉어과 어류가 24종으로 전체의 72.7%와 1531개체로 전체의 97.5%를 차지하였다. 전체 종에 대해 0.2%보다 적은 종은 L. andersoni, G. brevibraba, A. koreensis, C. rotundicaudata와 S. microdorsalis를 포함한 11종이었다. 박대천 수계에서 우점종은 상대풍부도가 28.66%로 Zacco temmincki가 우점하였고, Zacco platypus (28.28%)와 C. splendidus (12.55%)가 아우점하였다. 박대천의 각 지류에서, Zacco temmincki는 6개 지점에서, Zacco temmincki는 5개 지점에서 우점하였다. 박대천 수계로부터 총 33종 중 6과 14속 18종(54.5%)이 한국고유종이었다. 박대천에서 확인된 어류의 전체 군집 구조를 분석한 결과, 우점도 3.766, 다양도 0.903, 풍부도 10.012, 그리고 균등도는 0.594이었다. Sampes of the Bakdae-Cheon Stream, the upper Namhan-Gang River of Korea were collected at 11 stations from April to September 2001, and analyzed for the species composition, fish fauna, and species diversity. A total of 33 species (1570 individuals) and 25 genera belonging to seven families were collected during the study period. Cyprinid fishes occupied 72.7% of total 24 species and 97.5% of total 1531 individuals, respectively. The rare species which relative abundance is less than 0.2% of all were 1531 individuals, respectively. The rare species which relative abundance is less than 0.2% of all were about 11 species including Liobagrus andersoni, Gobiobotia brevibraba, Acheilognathus koreensis, Cobitis rotundicaudata, and Silurus microdorsalis. Dominant species in the Bakdae-cheon Stream was Zacco temmincki (relative abundance 28.66%), and subdominant species were Zacco platypus (28.28%), and Coreoleuciscus splendidus (12.55%). In each trivutaries of the Bakdae-cheon Stream, Z. temmincki was dominant species at six stations, and Z. platypus was dominant at five stations. Eighteen Species and 14 genera belonging to six families (51.5%) of total 33 species from the Bakdae-cheon Stream were well known as Korean endemic species. The species dominance (λ), diversity (H'), richness (RI), and evenness index (EI) of the fish community were 3.766, 0.903, 10.012, and 0.594, respectively.
朴涇錫 慶熙大學校 1984 論文集 Vol.13 No.-
This paper deals with an experimental study on the influence of fuel behavior in intake manifold for the performance of a low compression spark ignition engine. In this study, we knew that liquid-film fuel in intake manifold has a remarkable influence on the engine performance. And, from analysis of indicator pressure diagram, the value of combustion characteristics were obtained.
119구급차로 이송된 환자의 병원 전 전문응급처치에 관한 조사
박진옥,이경희,노상균,최청숙 한국응급구조학회 2003 한국응급구조학회지 Vol.7 No.1
An analysis was performed to evaluate the prehospital advanced emergency care in Emergency Medical Service system(EMS) through the review of the 119 transport records of 190 patients who visited to Jecheon Seoul hospital emergency center at Chungpuk Jecheon via 119 system during the period from October 2002 to September 2003. The results were obtained as follows: 1. Among 190 patients, male were 127(66.8%) and female were 63(33.2%) and non-traumatic patients were 81(42.6%) and traumatic patients were 109(57.4%). 2. The analysis of prehospital care by Emergency Medical Technician(EMT) revealed that Airway maintenance and keeping oral airway(37.7%), oxygen supply(75.9%), Tourniquett or MAST(18.9%), Immobilization of neck or spine(94.7%), Immobilization of extremities(51.4%), wound bandage and dressing(25.0%), BCLS(12.1%) and Application of AED(2 patients). 3. The analysis of prehospital care by EMT revealed prehospital care was limited to keep of intubation or LMA, medication and IV insertion, insertion of gastric tube and ACLS. 4. There was no case of the notification to medical institute or consultation to doctor.
박인전,오미숙,백경주,정영철,김신규,박종안 조선대학교 전자정보통신연구소 2002 電子情報通信硏究所論文誌 Vol.5 No.1
In this paper, we have proposed an effective moving objects tracking method based on the motion information in the video sequence. First, we extract the moving objects using the processes of morphological dilation and differential image in the video sequence and then we extract the motion information utilizing the location and orientation of the moving objects in the subsequent images. The motion information is extracted using various statistical measurements, extracting the high-level features from the regions of pixels in the images. The measurements, such a s area, length, perimeter, orientation, elongation, compactness, moments of inertia, are used as the statistical and geometrical descriptors. The principle axes of an object on this features gives the local direction of the object, while the center of gravity gives the center position of the object. The angle between two principal axes of the moving objects in the consecutive images gives the geometrical direction of the moving object, while the displacement of the center of gravity, gives the distance covered by the moving objects. So we have extracted the global motion information using the principal axes and center of gravity. The simulation results on the test images are very good, where either the motion is very slow, or the frame rate is very high.
박용희,서지영,민평경,이숙희 효성여자대학교 가정대학 학도호국단 1986 家政大論集 Vol.5 No.-
The purpose of this study was to investigate on the clothing life to 109 dwellersliving in Gadukdo. The clothing life was grouped into 4 factors;purchase, wearing, management, disposal. The results were as follows ; 1. 45.9% of the dwellers appropriated less than ten thousand won for a purchaseof clothes. The purchase behavior is irrational, 76.2% of the dwellers purchasedgarments under the necessity. 2. The reason of buying and wearing a ready- made clothes is not emotional Point of view but an economical price. 3. 81.7% of the dwellers were discontented with price and durability on ready-ma-de clothes. Purchasing ready-made clothes, they didn't concerned about indication ofquality . 4. 78.9% of the dwellers occasionally wore Korean costume on a holiday. 49.5%of the dwellers answered that they took no interest in fashion. 5. A supply rate of a washing machine is 7.3%, 80.7% of the dwellers usedlaundry soap because of their low price. 6. As a reason of disposal the clothing, it was worn-out. 35.8% of the dwellers saidso. Only 10.1% of the dwellers answered that the garments were behind the fashion. That showes a tendency to be opposite with city dwellers.