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Park, Kyoung Ryoung,An, Jun Yop,Kang, Jung Youn,Lee, Jung-Gyu,Lee, Youngjin,Mun, Sang A,Jun, Chang-Duk,Song, Woo Keun,Eom, Soo Hyun Academic Press 2017 Biochemical and biophysical research communication Vol. No.
<P><B>Abstract</B></P> <P>EF-hand domain-containing protein D2/Swiprosin-1 (EFhd2) is an actin-binding protein mainly expressed in the central nervous and the immune systems of mammals. Intracellular events linked to EFhd2, such as membrane protrusion formation, cell adhesion, and BCR signaling, are triggered by the association of EFhd2 and F-actin. We previously reported that Ca<SUP>2+</SUP> enhances the F-actin-bundling ability of EFhd2 through maintaining a rigid parallel EFhd2-homodimer structure. It was also reported that the F-actin-bundling ability of EFhd2 is regulated by a phosphorylation-dependent mechanism. EGF-induced phosphorylation at Ser183 of EFhd2 has been shown to inhibit F-actin-bundling, leading to irregular actin dynamics at the leading edges of cells. However, the underlying mechanism of this inhibition has remained elusive. Here, we report the crystal structure of a phospho-mimicking mutant (S183E) of the EFhd2 core domain, where the actin-binding sites are located. Although the overall structure of the phospho-mimicking mutant is similar to the one of the unphosphorylated form, we observed a conformational transition from ordered to disordered structure in the linker region at the C-terminus of the mutant. Based on our structural and biochemical analyses, we suggest that phosphorylation at Ser183 of EFhd2 causes changes in the local conformational dynamics and the surface charge distribution of the actin-binding site, resulting in a re-coordination of the actin-binding sites in the dimer structure and a reduction of F-actin-bundling activity without affecting the F-actin-binding capacity.</P> <P><B>Highlights</B></P> <P> <UL> <LI> EFhd2 is an actin-binding protein in the central nervous and immune systems of mammals. </LI> <LI> We created a core domain phospho-mimicking mutant of the EFhd2 (<SUB>CD</SUB>EFhd2<SUP>S183E</SUP>). </LI> <LI> The crystal structure of <SUB>CD</SUB>EFhd2<SUP>S183E</SUP> was solved at 1.86 Å. </LI> <LI> S183E changes the conformational dynamics and the surface charge distribution of the actin-binding sites. </LI> <LI> We propose a model for the phosphorylation-dependent modulation of actin dynamics by EFhd2. </LI> </UL> </P>
지속유량성 지속적 기도양압 장치와 요구유량성 지속적 기도양압 장치가 호흡 기능에 미치는 효과의 비교
박영주,이경민,엄대자,곽노길,최령,엄기현 대한마취과학회 1996 Korean Journal of Anesthesiology Vol.31 No.1
Background: Continuous positive airway pressure(CPAP) during spontaneous breathing has contributed greatly to the management of the neonatal respiratory distress syndrome and adult respiratory failure associated with acute lung disease. CPAP systems may be grouped into two general categories, demand flow CPAP system and continuous flow CPAP system. The purpose of this study was to evalute the respiratory effects of continuous flow CPAP system compared with demand flow CPAP system. Methods: Cardiorespiratory values were measured in twelve respiratory failure patients on demand flow CPAP system and continuous flow CPAP system made by authors. CPAP level and FIO were maintained at the same level for both modalities. Results: Changing from demand flow CPAP to continuous flow CPAP was associated with significant decrease in tidal volume(demand flow CPAP 450±153.0 ml, continuous flow CPAP 338±73.8 ml), airway pressure fluctuation(demand flow CPAP 6.4±1.2 cmH₂O, continuous flow CPAP 2.4±0.7 cmH₂O) and improvem arterial oxygen partial pressure(demand flow CPAP 90.0±20.9 mmHg, continuous flow CPAP 105.9±24.6 mmHg). There were no significant changes in other cardiorespiratory values, such as arterial blood pressure, heart rate and respiratory rate. Conclusions: These results suggest that continuous flow CPAP system may be a beneficial modality in the management of respiratory failure patients compared to demand flow CPAP system.
( Mi Ryoung Seo ),( Ji-won Kim ),( Eun-jung Park ),( Seung Min Jung ),( Yoon-kyoung Sung ),( Hyungjin Kim ),( Gunwoo Kim ),( Hyun-sook Kim ),( Myeung-su Lee ),( Jisoo Lee ),( Jian Hur ),( Bum Sik Chin 대한류마티스학회 2020 대한류마티스학회지 Vol.27 No.4
Patients with systemic rheumatic diseases (SRD) are vulnerable for coronavirus disease (COVID-19). The Korean College of Rheumatology recognized the urgent need to develop recommendations for rheumatologists and other physicians to manage patients with SRD during the COVID-19 pandemic. The working group was organized and was responsible for selecting key health questions, searching and reviewing the available literature, and formulating statements. The appropriateness of the statements was evaluated by voting panels using the modified Delphi method. Four general principles and thirteen individual recommendations were finalized through expert consensus based on the available evidence. The recommendations included preventive measures against COVID-19, medicinal treatment for stable or active SRD patients without COVID-19, medicinal treatment for SRD patients with COVID-19, and patient evaluation and monitoring. Medicinal treatments were categorized according to the status with respect to both COVID-19 and SRD. These recommendations should serve as a reference for individualized treatment for patients with SRD. As new evidence is emerging, an immediate update will be required. (J Rheum Dis 2020;27:218-232)