서울시내 치과의원의 소독기 실태

권주현,김성태,김광만,김경남 대한치과기재학회 1997 대한치과재료학회지 Vol.24 No.2

Sterilizer is one of the necessary equipments in dental clinics for aseptic treatment. There are many types of sterilizer, such as autoclave, chemiclave, dry heat sterilizer, UV(Ultra Violet) sterilizer, boiling sterilizer, EO(Ethylene Oxide) gas sterilizer, by sterilizing mechanis. To evaluate the type and sterilizing ability, one surveyer visited 100 dental clinics in Seoul and surveyed some items, such as age and gender of dentist, type, location and using period of sterilizer, how many sterilizing time per day and who operate sterilizer, so on. As preacticall operation the sterilizer, sterilized basillus subtilis in test tube and incubated it. Then it's turbidity was observed. The results were as follows 1. The descending order of plenty were autoclave(68), UV sterilizer(26), chemiclave(12), dry heat sterilizer(4) and boiling sterilizer(2). 2. The descending order of sterilizing ability were dry heat sterilizer(100%), autoclave(89%), chemicloave(73%), UV sterilizer(43%) and boiling sterilizer(0%). 3. Sterilizing ability in dental clinics that have both sterilizer, autoclave and UV sterilizer, chemiclave and UV sterlilzer was low than single sterilizer(autoclave, chemiclave). 4. The descending order of location of sterilizer were treatment room, laboratory room, sterilizing room and the order of operator were assistant, hygiene and both assistant and hygiene.

고등급 이형성을 보인 편평톱니샘종

권경주,정성애,심기남,정정화,강석형,송도경,전승정,김혜인 이화여자대학교 의과학연구소 2012 EMJ (Ewha medical journal) Vol.35 No.1

Until recently, colorectal polyps were classified predominantly as hyperplastic or adenomatous. While adenomatous polyps are well-characterized precursor lesions of adenocarcinomas, hyperplastic polyps have been considered as benign lesion. However, some hyperplastic polyps with serrated morphology of the crypts have been recognized to have distinctive features and these polyps were termed ‘serrated adenomas’. Recent data show that sessile serrated adenomas (SSA) might be the precursors of serrated colonic cancers, underlining the necessity of identifying them. SSA is approximately 3% of all polyps, commonly appears as flat or sessile and yellowish due to mucus production. In the pathogenesis of SSA, progression to high grade dysplasia or early invasive carcinoma may be associated with serrated neoplasia pathway different from adenoma-carcinoma sequence. We report a case with a colon polyp diagnosed as sessile serrated adenoma with high grade dysplasia after endoscopic submucosal dissection.

국내 살모넬라 분리주에서 spvR 유전자 분포와 단일 뉴클레오티드 다형 연구

권혁준,박경윤,박주연,박용호,김선중,유한상 대한감염학회 2004 감염과 화학요법 Vol.36 No.6

목적 : 지금까지 병원성 살모넬라를 확인하기 위해 분자량이 큰 플라스미드의 존재 유무를 확인하여 왔으나 살모넬라 혈청형 및 검출 방법에 따라 양성율에 큰 차이를 보이고 있고, 일부 병원성 살모넬라 분리주에서 병원성 플라스미드가 관찰되지 않아 spv 유전자들의 유무에 대한 아른 확인방법이 필요하다. 본 실험은 국내 동물 유래 주요 살모넬라 혈청형에서 spvR 유전자의 분포를 알아보고, 병원성 살모넬라 분자진단을 위한 분자표지로써의 가치를 평가하기 위해 실행하였다. 재료 및 방법 : 국내 가축 유래 S. lyphimurium (ST, 26주), S. enteritidis (SE, 10주), S. pullorum (SP, 40주), S. gallinarum (SG, 53주)에 대해 GenBank에 등록된 ST, SE, SP의 spvR 유전자를 비교하여 ST와 SE 간에 차이를 보이는 single nucleotide polymorphism (SNP, 625번 뉴클레오티드)을 포함하도록 시발자를 합성하여 집락-PCR을 수행하였다. 증폭 산물(194bp)은 자동 염기서열장치를 이용하여 염기서열을 결정하였고, 제한효소인 MseI을 사용하여 PCR-RFLP를 실시하였다. 결과 : 집락-중합효소연쇄반응 결과 SP, SG, SE의 모든 (100%) 분리주에서 특이 증폭산물이 검출되었으나 ST의 경우 19주(73%)에서만 증폭되었다. 특히 병원성 관련 플라스미드가 관찰되지 않았던 SP 4주에서 특이적인 증폭산물이 검출되었다. 염기서열 분석결과 SE, SG, SP는 625번 뉴클레오티드에 아데닌을, ST는 구아닌을 가지고 있어 PCR-RFLP를 이용하여 쉽게 구분할 수 있었다. 결 론 : spvR에 대한 집락-중합효소연쇄 반응법은 병원성 플라스미드 검출법보다 SE, SP, SG 병원성 주를 신속하게 검출하는데 유용하고, 625번 뉴클레오티드의 SNP는 ST와 SE, SG, SP를 구분하는 분자표지로 사용될 수 있을 것으로 기대되며, ST에 비해 SE는 진화적으로 SG와 SP에 가까운 것으로 생각되었다. Background : The Salmonella virulence plasmid (spv) genes (spvR, A, B, C and D) on the large virulence plasmids of pathogenic Salmonella serotypes can replace the virulence of the whole plasmid. Recently, virulence plasmid-negative pathogenic Salmonella isolates were isolated. However, positive rates of spv genes among Korean Salmonella serotypes have been obscure. spv genes are conserved in compared to other virulence genes but there are single nucleotide polymorphisms (SNPs) conserved in only certain serotype. Such SNPs are useful for differentiation and understanding evolution of certain serotypes. Materials and Methods : Salmonella serotypes isolated from live stocks [Salmonella typhimurium (ST, 26), S. enteritidis (SE, 10), S. gallinarum (SG, 40) and S. pullorum (SP, 53)] were used for colony-PCR. A primer set covering single nucleotide polymorphism (SNP) at 625th nucleotide of spvR was designed. The nucleotide sequences of amplicons were determined by cyclic sequencing method and RFLP was performed by using MseI. Results : All isolates of SE, SG and SP, including four plasmid-negative isolates, showed specific amplicons but not all of ST (19/26, 73%) were positive to spvR. Based on the nucleotide sequence of 625th nucleotide and PCR-RFLP, SE, SG and SP [A(625)] and ST [G(625)] could be differentiated. Conclusion : spvR can be used as a molecular marker to detect virulent SE, SG, SP and the SNP may be useful for differentiation of SE, SG, SP and ST. According to the SNP study SE may be evolutionarily closer to SG and SP than ST.

악성 혈액질환에서 성공적인 동종골수이식 후 숙주 기질 미세환경의 구축

조상희,이제중,남찬은,최경상,정익주,이일권,김진희,박종태,김형준 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.1

인체의 골수는 간엽모세포를 함유하고 있으며 이들은 골수미세환경의 주된 세포들로 분화가 가능하여 조혈기능을 지지한다. 본 연구에서는 성별이 다른 동종조혈모세포이식 환경에서 조혈모세포의 완전 생착을 보이고, 이식 후 1년에서 8년이 지난 11예의 재생불량성 빈혈 및 백혈병 환자들을 대상으로 하여 골수에서 MSC를 분리하고 체외 확장을 통해 배양된 MSC에서 X 염색체 탐식자를 이용한 FISH 및 microsatellite polymorphism PCR 기법으로 그 기원을 확인하였다. 그 결과 조혈모세포는 완전히 공여자 기원으로 대치되었음에도 불구하고 MSC는 모두 수여자 기원임을 알 수 있어, 동종조혈모세포이식에서 미세환경의 구축은 수여자의 자가 생산에 의한 골수 간질세포에 의한 것으로 생각된다. Background: Human bone marrow (BM) contains mesenchymal stem cells (MSC) that can differentiate into various cells of mesenchymal origin. It remains a matter of controversy whether donor-derived stromal cells are capable of engraftment following hematopoietic stem cell transplantation (HSCT) or not. Methods: To determine if donor-derived stromal cells are transferred to the recipients of allogeneic HSCT, we investigated the characterization of MSC in 11 patients 1 to 8 years after sex mis-matched allogeneic HSCT in severe aplastic anemia and leukemia. Results: All patients had complete engraftment with donor- derived stem cells as shown by detection of donor type DNA in peripheral blood mononuclear cells. Following culture, MSC showed the expression of SH2 and SH4, but none of the hematopoietic markers of CD14, CD34, or CD45. MSC which can be differentiated to osteogenic lineage showed the genotype of recipient completely using FISH or PCR analysis. Conclusion: This study confirmed that MSC isolated from recipients of allogeneic HSCT in severe aplastic anemia and leukemia are not of donor genotype despite of full hematopoietic engraftment with donor type. Donor cells did not contribute to reconstitute the marrow microenvironment.

증례 : 호흡기 ; 간질성 폐부종으로 발현한 좌심방 점액종 1예

권경주 ( Kyoung Joo Kwon ),김고흔 ( Ko Hun Kim ),천은미 ( Eun Mi Chun ),류연주 ( Yeon Ju Ryu ),이진화 ( Jin Wha Lee ),장중현 ( Joong Hyun Jang ),심성신 ( Sung Shin Sim ) 대한내과학회 2010 대한내과학회지 Vol.79 No.2

심장의 원발성 종양은 드문 질환으로, 대부분의 경우 점액종이다. 심장의 점액종은 중추신경계와 혈관계통에 발생하는 색전의 원인이나 그 임상 증상은 비특이적이다. 비특이적인 전신 증상과 경미한 색전에 의한 증상은 심장 병력이 없는 환자에서 쉽게 지나칠 수 있다. 그러나 치명적인 색전을 예방하기 위해서는 점액종의 임상증상에 대한 이해와 적절한 치료가 필요하다. 저자들은 간질성 폐부종 증상으로 내원하여 좌심방 점액종을 진단받고 수술을 앞두고 있던 중다발성 색전으로 인해 사망한 환자 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Primary neoplasm of the heart is rare. Benign tumors, the majority of which are myxomas, comprise up to 50% of cardiac neoplasms. Although cardiac myxoma is a source of emboli to the central nervous system and elsewhere in the vascular tree, its clinical signs and symptoms may be nonspecific. These nonspecific systemic symptoms and minor embolic phenomena may be overlooked in patients without a previous history of cardiac problems. Thus, we need to recognize the signs of myxoma and begin treatment immediately. We encountered a case of left atrial myxoma, presenting as pulmonary interstitial edema of unknown cause, in a 50-year-old female patient. Excision of a mass located on the left atrium was scheduled but she developed a cerebral embolism that led to her death. (Korean J Med 79:191-194, 2010)

Regulation and Destabilization of HIF-1α by ARD1-Mediated Acetylation

Jeong, Joo-Won,Bae, Moon-Kyoung,Ahn, Mee-Young,Kim, Se-Hee,Sohn, Tae-Kwon,Bae, Myung-Ho,Yoo, Mi-Ae,Song, Eun Joo,Lee, Kong-Joo,Kim, Kyu-Won 梨花女子大學校 藥學硏究所 2002 藥學硏究論文集 Vol.- No.11

Hypoxia-inducible factor 1 (HIF-1) plays a central rolein cellular adaptation to changes in oxygen availability.Recently. prolyl hydroxylation was identified as a keyregulatory event that targets the HIF-1α subunit forproteasomal degradation via the PVHL ubiquitinationcomplex. In this report we revea1 an important func-tion for ARD1 in mammalian cells as a protein acetyl-transferase by direct binding to HIF-1α to regulate itsstability. We present further evidence showing thatARD1 -mediated acetylation enhances interaction ofHIF-1α with PVHL and HIF-lαubiquitination, sug-gesting that the acetylation of HIF-lαby ARD1 is criti-cal to proteasomal degradation. Therefore, we haveconcluded that the role of ARD1 in the acetylation ofHIF- 1α provides a key regulatory mechanism underly-ing HIF-1αstability.

한국 여성에서 무월경의 원인적 분류

권수경 ( Su Kyoung Kwon ),이선주 ( Sun Joo Lee ),전은진 ( Eun Jin Jun ),이경희 ( Kyoung Hee Lee ),유래미 ( Rae Mi Ryu ),강혁재 ( Hyuk Jae Kang ),김성훈 ( Sung Hun Kim ),채희동 ( Hee Dong Chae ),김정훈 ( Chung Hoon Kim ),강병문 ( B 대한산부인과학회 2011 Obstetrics & Gynecology Science Vol.54 No.10

목적 한국 여성에서의 무월경의 원인적 분류를 알아보고자 하였다. 연구방법 1998년 1월부터 2008년 5월까지 울산대학교 의과대학 서울아산병원 산부인과를 방문한 741명의 무월경 환자를 대상으로 의무기록을 후향적으로 분석하여 연구하였다. 환자는 10-20세를 group Ⅰ으로 분류하였고, 21-30세를 group II, 31-40세를 group III로 분류하여 세 그룹으로 나누어 연구하였다. 무월경 환자는 원발성 무월경과 속발성 무월경 환자로 나누었다. 결과 원발성 무월경 환자는 89명(12.0%)이었고, 속발성 무월경 환자는 652명(88.0%)이었다. 10-20세 그룹에서는 원발성 무월경 환자는 62명(22.6%)이었고, 속발성 무월경 환자는 212명(77.4%)이었다. 21-30세 그룹에서는 원발성 무월경이 27명(6.5%)이었으며, 속발성 무월경은 389명(93.5%)이었다. 31-40세 그룹의 무월경 환자에서는 원발성 무월경 환자는 없었고, 속발성 무월경 환자만 51명(100%)이었다. 10-20세 그룹에서 원발성 무월경의 원인은 뮐러관 무발육증후군, Turner 증후군 순이었고, 21-30세 그룹에서도 동일하였다. 10-20세 그룹에서 속발성 무월경의 원인은 다낭성난소증후군, 체중관련 저성선자극호르몬성 저성선증, 조기난소부전 순이었고, 21-30세 그룹에서도 동일하였다. 31-40세 그룹에서는 조기난소부전, 다낭성난소증후군 순이었다. 결론 본 연구에서 한국여성의 전 연령군에서 원발성 무월경의 흔한 원인은 뮐러관 무발육증후군, Turner 증후군 순이었고, 속발성 무월경의 원인은 다낭성난소증후군, 조기난소부전, 체중관련 저성선자극호르몬성 저성선증 순이었다. Objective To investigate the causes of amenorrhea in Korean women. Methods A total of 741 patients with amenorrhea who visited the department of Obstetrics and Gynecology, Asan Medical Center were included in this study between January 1998 and May 2008. Patients were divided into three groups age 10-20 was defined as group I, age 21-30 was group II and age 31-40 was group III. Amenorrhea was categorized into the primary amenorrhea and secondary amenorrhea. Results The number of patients with primary amenorrhea was 89 (12.0%) and secondary amenorrhea was 652 (88.0%). The number of patients with primary amenorrhea was 62 (22.6%) and secondary amenorrhea was 212 (77.4%) in age 10-20 group. In age 21- 30 group, the number of primary amenorrhea was 27 (6.5%) and secondary amenorrhea was 389 (93.5%). In age 31-40 group, the number of secondary amenorrhea was 51 (100%). The causes of primary amenorrhea in group I were as follows; Mayer-Rokitansky-Kuster-Hauser (MRKH) syndrome (17.7%, 11/62), Turner syndrome (11.3%, 7/62), constitutional delay (9.7%, 6/62). In group II; MRKH syndrome (18.5%, 5/27), Turner syndrome (18.5%, 5/27), and Idopathic hypogonadotropic hypogonadism (14.8%, 4/27). The causes of secondary amenorrhea in group I were as follows; Polycystic ovarian syndrome (PCOS; 60.8%, 129/212), weight related hypogonadotropic hypogonadism (12.3%, 26/212), premature ovarian failure (POF; 9.9%, 21/212). In group II; PCOS (62.5%, 243/389), weight related hypogonadotropic hypogonadism (9.8%, 38/389), POF (9.3%, 36/389). In group III; POF (43.1%, 22/51), PCOS (17.6%, 9/51). Conclusion The common cause of primary amenorrhea were MRKH syndrome, Turner`s syndrome, and the common cause of secondary amenorrhea were PCOS, POF, weight related hypogonadotropic hypogonadism.

Effect of Different Rootstocks on Wilting Occurrence, Plant Growth, and Fruit Quality of Melon

Joo Hyun Lee(이주현),Joon Kook Kwon(권준국),Kyoung Sub Park(박경섭),Yun Chan Huh(허윤찬),Chai Il Lim(임채일),Dong Kum Park(박동금),Kwan Dal Ko(고관달) 한국원예학회 2009 원예과학기술지 Vol.27 No.2

무네트멜론 ‘홈런스타’와 네트멜론 ‘얼스엘리트’를 ‘강건토좌’, ‘신토좌’, ‘특토좌’, ‘조이너스’, ‘레인보우’, ‘에이스’, ‘엘리트’ 등의 7종류의 대목에 접목한 접목묘와 무접목묘를 공시하여 시듦증 발생, 생육, 수량, 과실당도, 유리당 함량 등을 조사하였다. 시듦증 발생은 ‘홈런스타’ 무접목묘 100%, 대목 ‘레인보우’ 83%, ‘엘리트’ 55%, ‘조이너스’ 22%, ‘신토좌’ 16%가 발생하였고 ‘강건토좌’, ‘특토좌’, ‘에이스’ 대목에서는 발생되지 않았다. ‘얼스엘리트’는 ‘레인보우’ 72%, ‘엘리트’와 ‘조이너스’에서 6% 발생하였으며, 다른 대목에서는 발생하지 않았다. ‘홈런스타’의 과중은 ‘강건토좌’, ‘신토좌’, ‘특토좌’ 대목에서 높고, ‘얼스엘리트’는 접목처리에서 높은 경향을 보여 ‘에이스’ 대목에서는 실생에 비해 과중이 28% 증가하였다. ‘홈런스타’ 과실의 가용성 당함량은 실생이 가장 높았으며, 시듦증 발생이 많았던 ‘조이너스’와 ‘레인보우’ 대목에서 유의적으로 함량이 낮았고, 다른 대목간에는 차이가 없었다. ‘얼스엘리트’의 가용성 당함량은 ‘에이스’ 대목에서 가장 높았고, 무접목묘, ‘특토좌’, ‘신토좌’, ‘강건토좌’ 대목 순으로 높았으며, ‘조이너스’, ‘레인보우’, ‘엘리트’에서 유의적으로 낮았다. ‘홈런스타’는 실생에서 ‘얼스엘리트’는 ‘에이스’ 대목에서 유리당 함량이 유의적으로 높게 나타났다. 환원성 당인 sucrose대비 비환원성 당인 glucose와 fructose의 합의 비율(S/G+F)은 홈런스타 대목재배에서 2.2-2.5, 실생재배 1.5로 접목재배가 실생에 비해 과실내 환원당인 glucose와 fructose의 비율이 낮은 경향을 보였다. 멜론 두 품종의 접목재배에서 시들음증 경감, 수량 및 과실 품질 등을 고려할 때, ‘홈런스타’는 ‘강건토좌’ 대목이 ‘얼스엘리트’는 ‘에이스’ 대목이 적합하였다. Key growing characteristics of melon cultivars, ‘Homerunstar’ and ‘Earl’s elite’ were investigated after they were grafted onto Cucurbita rootstocks ‘Gangguntozwa’, ‘Shintozwa’, ‘Teuktozwa’, ‘Joinus’, ‘Rainbow’, ‘Ace’, and ‘Elite’. Significantly different results were obtained in wilting incidence, plant growth, fruit quality and fruit yield depending on various rootstocks. No wilting symptom was observed when cultivar ‘Homerunstar’ was grafted onto ‘Gangguntozwa’, ‘Teuktozwa’ and ‘Ace’ rootstocks, whereas the symptom occurrence was 83, 55, 22, and 16%, respectively, when grafted onto ‘Rainbow’, ‘Elite’, ‘Joinus’, and ‘Shintozwa’. Occurrence of wilting symptom was 100% when the cultivar was grown without grafting. The occurrence of wilting symptom in cultivar ‘Earl’s elite’ was 6% when it was grafted onto ‘Elite’ and ‘Joinus’ rootstocks, whereas it was 72% when the cultivar was grafted onto ‘Rainbow’ rootstocks. The highest soluble-solid content (°Brix) was observed when cultivar ‘Earl’s elite’ was grafted onto ‘Ace’ or ‘Joinus’. When the cultivar ‘Homerunstar’ was grown without grafting, the soluble-solid content was 1 °Brix higher than that of grafted one; however, a higher ratio of sucrose to glucose+fructose (S/G+F) was observed in the fruit. Total fruit yield was higher when the two cultivars were grafted onto ‘Gangguntozwa’, ‘Shintozwa’, ‘Teuktozwa’, and ‘Ace’ rootstocks than those of non-grafted ones. These results suggest that the best rootstock for cultivar ‘Homerunstar’ is ‘Teuktozwa’ and for cultivar ‘Earl’s elite’ is ‘Ace’.

Korean Red Ginseng Extract inhibits Tumor Necrosis Factor-alpha-induced Monocyte Adhesion in the Human Endothelial Cells

Joo, Hee-Kyoung,Lee, Sang-Ki,Kim, Hyo-Shin,Song, Yun-Jeong,Kang, Gun,Park, Jin-Bong,Lee, Kwon-Ho,Cho, Eun-Jung,Lee, Jae-Hwan,Seong, In-Whan,Kim, Se-Hoon,Cho, Chung-Hyun,Jeon, Byeong-Hwa The Korean Society of Ginseng 2008 Journal of Ginseng Research Vol.32 No.3

Vascular inflammation is an important step in the development of cardiovascular disorder. Since it has not been known whether Korean red ginseng has a role to play on the vascular inflammation, we investigated the effects of Korean red ginseng extract (KRGE) on monocyte adhesion and its underlying signaling mechanism. Monocyte adhesion assay and Western blot were conducted on the human umbilical vein endothelial cells to study monocyte adhesion and the expression of adhesion molecules. Intracellular calcium was measured with Fura-2 fluorescent staining, and superoxide production was measured with lucigenin chemiluminescence in the endothelial cells. KRGE inhibits tumor necrosis factor (TNF)-alpha-induced monocyte adhesion on the endothelial cells at the range of $0.03{\sim}1$ mg/ml. TNF-alpha-induced vascular cell adhesion molecule-1 and intercellular cell adhesion molecule-1 expression were inhibited by the pretreatment of KRGE in the endothelial cells. KRGE also inhibits TNF-alpha-induced intracellular calcium and the superoxide production in the endothelial cells. This study first demonstrated that KRGE inhibits TNF-alpha-induced monocyte adhesion by inhibiting the adhesion molecule expression, intracellular calcium and superoxide production in the endothelial cells. Therefore, the anti-inflammatory function of KRGE may be contributed to protecting the endothelial dysfunction in the vascular inflammatory disorders.

Changes of SGOT and SGPT After Halothane, Enflurane and Thalamonal Anesthesia

Kwon, Ou-Kyoung,Shim, Jae-Yong,Park, Chong-Min,Suh, Jae-Hyun,Park, Chul-Joo Catholic Medical Center 1984 Bulletin of the Clinical Research Institute Vol.12 No.-