Morin exerts cytoprotective effects against oxidative stress in C2C12 myoblasts via the upregulation of Nrf2-dependent HO-1 expression and the activation of the ERK pathway

Lee, Moon Hee,Han, Min Ho,Lee, Dae-Sung,Park, Cheol,Hong, Su-Hyun,Kim, Gi-Young,Hong, Sang Hoon,Song, Kyoung Seob,Choi, Il-Whan,Cha, Hee-Jae,Choi, Yung Hyun UNKNOWN 2017 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.39 No.2

<P>In the present study, we investigated the cytoprotective efficacy of morin, a natural flavonoid, against oxidative stress and elucidated the underlying mechanisms in C2C12 myoblasts. Our results indicated that morin treatment prior to hydrogen peroxide (H2O2) exposure significantly increased cell viability and prevented the generation of reactive oxygen species. H2O2-induced comet-like DNA formation and gamma H2AX phosphorylation were also markedly suppressed by morin with a parallel inhibition of apoptosis in C2C12 myoblasts, suggesting that morin prevented H2O2-induced cellular DNA damage. Furthermore, morin markedly enhanced the expression of heme oxygenase-1 (HO-1) associated with the induction and phosphorylation of nuclear factor-erythroid 2-related factor 2 (Nrf2) and the inhibition of Kelch-like ECH-associated protein 1 (Keapl) expression. Notably, these events were eliminated by transient transfection with Nrf2-specific small interfering RNA. Additional experiments demonstrated that the activation of the Nrf2/HO-1 pathway by morin was mediated by the extracellular signal-regulated kinase (ERK) signaling cascade. This phenomenon was confirmed with suppressed Nrf2 phosphorylation and consequently diminished HO-1 expression in cells treated with a pharmacological inhibitor of ERK. Collectively, these results demonstrated that morin augments the cellular antioxidant defense capacity through the activation of Nrf2/HO-1 signaling, which involves the activation of the ERK pathway, thereby protecting C2C12 myoblasts from H2O2,-induced oxidative cytotoxicity.</P>

대학 운동선수의 정체성과 성취목표가 수행전략에 미치는 효과

유경호(Yoo, Kyoung-Ho),장세용  (Jang, Se-Yong),최경아(Choi, Kyung-A),김승철(Kim, Seung-Chul) 한국체육과학회 2013 한국체육과학회지 Vol.22 No.1

The aim of this study was to examine the effect of college athletes’ achievement goal and identity on performance strategy. The participants of this study were 300 of college athletes in Seoul and Kyoung-gi area. For the analysis of data, exploratory factor analysis, multivariate analysis of variance (MANOVA), two-way ANOVA, and hierarchical multiple regression were used. The results were as follows. First, the significantly important predictor variables of athletic identity were condition control, emotion control, self-talk, and imegery/goal setting predictor variable. Condition control, tense relaxation, and self-talk were significantly important predictor variables of achievement goal. Condition control, emotion control, and imegery/goal setting were significantly important predictor variables of athletic career, skillfulness, and performance avoid goal Two, athletic identity, and performance approach goal interacted and affected self-talk for the athletes among two-way, and three-way interaction variables.

Sauchinone Suppresses Pro-inflammatory Mediators by Inducing Heme Oxygenase-1 in RAW264.7 Macrophages

Li, Bin,Lee, Dong-Sung,Choi, Hyun-Gyu,Kim, Kyoung-Su,Kang, Dae-Gil,Lee, Ho-Sub,Jeong, Gil-Saeng,Kim, Youn-Chul Pharmaceutical Society of Japan 2011 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.34 No.10

<P>Sauchinone, a biologically active lignan isolated from the roots of <I>Saururus chinensis</I> (L<SMALL>OUR</SMALL>.) B<SMALL>AILL</SMALL>. (Saururaceae), is reported to exert a variety of biological activities, such as hepatoprotective, anti-inflammatory actions and inhibitory effects on bone resorption. In this study, we investigated the effect of sauchinone in suppressing cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expression, leading to a reduction in COX-2-derived prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>) and iNOS-derived nitric oxide (NO) production in lipopolysaccharide (LPS) stimulated RAW264.7 macrophages. Present study also demonstrates the effects of sauchinone in inducing heme oxygenase-1 (HO-1) expression and an increase in heme oxygenase (HO) activity in RAW264.7 macrophages. The effects of sauchinone on LPS-induced PGE<SUB>2</SUB>, NO, tumor necrosis factor-α (TNF-α) and interlukine-1β (IL-1β) production were partially reversed by the HO-1 inhibitor Tin protoporphyrin was also seen in this study. In addition, we found that treatment with extracellular signal-regulated kinase (ERK) inhibitor (PD98059) reduced sauchinone-induced HO-1 expression. Sauchinone also increased ERK phosphorylation. These results suggest that sauchinone inhibits pro-inflammatory mediators through expression of anti-inflammatory HO-1 <I>via</I> ERK pathway.</P>

Sulforaphane protects against acetaminophen-induced hepatotoxicity

Noh, Jung-Ran,Kim, Yong-Hoon,Hwang, Jung Hwan,Choi, Dong-Hee,Kim, Kyoung-Shim,Oh, Won-Keun,Lee, Chul-Ho Elsevier 2015 Food and chemical toxicology Vol.80 No.-

<P><B>Abstract</B></P> <P>Oxidative stress is closely associated with acetaminophen (APAP)-induced toxicity. Heme oxygenase-1 (HO-1), an antioxidant defense enzyme, has been shown to protect against oxidant-induced tissue injury. This study investigated whether sulforaphane (SFN), as a HO-1 inducer, plays a protective role against APAP hepatotoxicity <I>in vitro</I> and <I>in vivo</I>. Pretreatment of primary hepatocyte with SFN induced nuclear factor E2-factor related factor (Nrf2) target gene expression, especially HO-1 mRNA and protein expression, and suppressed APAP-induced glutathione (GSH) depletion and lipid peroxidation, which eventually leads to hepatocyte cell death. A comparable effect was observed in mice treated with APAP. Mice were treated with 300 mg/kg APAP 30 min after SFN (5 mg/kg) administration and were then sacrificed after 6 h. APAP alone caused severe liver injuries as characterized by increased plasma AST and ALT levels, GSH depletion, apoptosis, and 4-hydroxynonenal (4-HNE) formations. This APAP-induced liver damage was significantly attenuated by pretreatment with SFN. Furthermore, while hepatic reactive oxygen species (ROS) levels were increased by APAP exposure, pretreatment with SFN completely blocked ROS formation. These results suggest that SFN plays a protective role against APAP-mediated hepatotoxicity through antioxidant effects mediated by HO-1 induction. SFN has preventive action in oxidative stress-mediated liver injury.</P> <P><B>Highlights</B></P> <P> <UL> <LI> SFN pretreatment increases the cell viability against APAP-induced toxicity. </LI> <LI> SFN pretreatment protects depletion of cellular GSH after APAP treatment. </LI> <LI> SFN pretreatment enhances Nrf2 target gene expression, especially HO-1 after APAP treatment. </LI> <LI> SFN has protective effect against APAP overdose-induced liver injury <I>in vivo</I> model as well. </LI> </UL> </P>

Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

체외 충격파 쇄석기에 의해 발생된 음향 흐름 현상 연구

최민주,이승환,홍경진,김호찬,박재우,이헌주 제주대학교 공과대학 첨단기술연구소 2001 尖端技術硏究所論文集 Vol.12 No.2

Acoustic streaming produced by an extracorporeal shock wave lithotripter was visualized for the first time in the present study. It was observed that the streaming velocity increased with the shock wave amplitude and was reaching up to 3 cm/s at the highest output setting of the lithotripter.

대요근 근구 차단술의 해부학적 고찰 및 임상 효과

신경호,김헌,최락민,김희상,안경회 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.2

천연 포도과즙 발효용 식초산균 분리

최경호,정은희 대구효성가톨릭대학교 응용과학연구소 1998 응용과학연구논문집 Vol.6 No.2

포도과즙을 이용한 식초산 생산을 위하여 강한 증식력과 아울러 아황산 소다 및 poly phenol성 물질에 대한 내성을 지니고 산소 요구도가 낮은 발효균주가 요망되었다. 본 연구에서는 이러한 성질을 갖춘 우수한 식초산균을 분리하기 위하여 실험을 수행하였으며 탄산칼슘을 첨가한 ethanol배지와 포도과즙을 이용하여 여러 가지 재래초로부터 식초산균을 분리하였다. 분리균은 단간균 내지 타원형 세균으로 불규칙한 모양을 하고 있으며, 전체적인 모양은 대조군으로 사용한 Acetobacter aceti(KCTC 1010)와 비슷하였으나 크기의 차이가 있다. 분리균의 증식도는 대조균보다 현저히 빨랐으며 paper disc법으로 확인한 clear zone 생성능력(Acetic acid 생성 능력과 비례)은 분리균이 대조균보다 약 11%가 높았다. 분리균은 포도과즙을 산도 6.2%의 포도식초로 발효하였으며, 8일간의 발효과정중 pH는 3.8에서 3.5로 약간 저하되었다. HPLC분석에 따른 발효전과 발효 후의 유기산의 변화는 tartaric acid와 fumaric acid가 약간 증가되었으며, acetic acid는 약 2.5배 증가하여 전체 유기산의 90%이상을 차지하였고 총 유기산의 함량도 증가하였다. 이상의 결과로 본 실험에서 분리한 식초산균은 천연 포도과즙에서 빠르게 증식하여 고동도(6%이상)의 식초산을 생성할 뿐만아니라 pH와 색상의 변화가 적고 정치배양에도 잘 적응하는 우수한 포도식초 발효균으로 판정되었다. Experiments were carried out to isolate a bacterial strain for fermentation of alcoholic grape juice(low grade wine) to vinegar without any drastic changes in color of the products. Among eight strains of bacterium isolated from various sources, a bacterial strain was finally selected as the fermenting organism by using solid-ethanol medium. Cells of the bacterium were rod or ellipsoid-shaped. Microscopic morphology of the cells were very resembled to Acetobacter aceti(KCTC 1010) used as an control organism. The bacterium grew actively in grape juices after a lag phase for 2 hours, and produced acetic acid in accordance with it's growth. Wine vinegar containning 6.2% acetic acid and having typical flavor and color of grape fruit was produced by fermentation for 8 days. These results suggest that the isolated bacterium was an Acetobacter sp. and useful for fermentation of wine vinegar of good quality.

양측성 관상동정맥루 1예

최용원,오석규,이재훈,이상재,권경희,최은경,김남호,정진원 圓光大學校 醫科學硏究所 2002 圓光醫科學 Vol.17 No.1

저자들은 호흡곤란, 흉부불쾌감, 심계항진 등을 주소로 내원한 환자에서 경흉부 및 경식도 심초음파 검사상 주폐동맥에서 이완기에 전행하는 지속적인 혈류의 흐름을 관찰하고, 관상동맥 조영술을 통해 좌전하행지 중간부위와 우관상동맥 근위부에서 각각 기시하여 공통경로를 이루면서 주폐동맥으로 유입되는 양측성 관상동정맥루를 보이는 드문 1예를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. A coronary arteriovenous fistula is infrequently encountered vascular communication between a coronary artery and a cardiac chamber, a great artery or the vena cava. It is the most common congenital anomaly that can affect coronary perfusion. Bilateral involvement of coronary fistula constitutes an uncommon subgroup of coronary arteriovenous fistulas. A 69 year-old female patient presented with chest discomfort, palpitation, and dyspnea. In the echocardiography, doppler color flow imaging visualized abnormal flow signals with mosaic appearance in the main pulmonary artery during diastolic phase. Coronary angiography revealed arteriovenous fistula arising from the left anterior descending artery and the right coronary artery. Both coronary arteriovenous fistulas drained into the main pulmonary artery. We report a case of bilateral coronary arteriovenous fistula that was confirmed by echocariography and coronary angiography.

Paraquat 처리에 의한 Bacillus megaterium의 Superoxide dismutase 활성저해

최경호,김춘숙,유지선 대구효성가톨릭대학교 1993 연구논문집 Vol.46 No.1

The effect of paraquat (1, 1'-dimethyl-4,4'-dipyridilium dichloride) treatment on activity of microbial superoxide dismutase(SOD), a key enzyme which protects the organism from toxicity of oxygen, was investigated. Exponentially growing cells of Bαcillus megaterium(ATCC 1987) were treated with 1.OmM paraquat by cultivation in the concomitant presence of the paraquat. Paraquat-treated culture showed a linear decrease in turbidity, cellular weight and vidable cell counts while those of control culture showed a linear increase until nine hours of cultivation. After nine hours of cultivation, turbidity(0.68), dry cell weight(1.19mg/ml) and viable cell counts(5.0×19?cells/ml) of the treated culture reached to approximately 62%, 83% and 4% of those of initial, respectively. Paraquat-treated culture(in vivo) showed a SOD activity of 0.68unit/ml and a specific activity of 1.79unit/mg protein after nine hours of cultivation. These values were equel to 22% and 44% of the control culture, respectively. When enzymes were liberated from sonic-disruptured cells(in vitro) by orderly using of sodium phosphate buffer, buffered lysoyme and triton X-100, paraguat-treated culture showed an increased activity of the engyme, while control culture did not show any significant increase of the enzyme activity. Activities of SOD drastically decreased as increasing the paraquat concentration in the crude enzyme lysate, although protein content was not significantly varied. The lysate dissolved 1.0mM paraquat showed a specific activity of SOD decreased to 45% of control lysate. It was noticed that the decreasing rate was approximately similar to that of the lysate prepared from paraquat-treated cells.