농작물 해충 및 진균류 방제를 위한 방선균의 분리 및 동정

이은정,강경돈,황교열,김두호,김신덕,성수일 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.1

Twenty seven out of ca. 5,000 actinomycete strains, which were isolated from soil collected throughout the country, showed antimicrobial effects against fungi, Rhizopus stronifer (ATCC6227a), Rhizoctonia solani (KCCM 11271) and yeast, Candida albicans(ATCC10231). From these antifungal microorganisms, we further selected seven strains which seemed to produce insecticidal substances with in vivo test, using silkworm, Bombyx mori and beet armyworm, Spodoptera exigua. Morphological and biochemical experiments revealed that three strains out of seven were streptomyces. Further investigations on the physical and chemical properties of these antifungal and insecticidal substances are now in progress.

뽕잎 함유 대두발효물이 신생 당뇨유도쥐에 미치는 혈당강하효과

황교열(Kyo-Yeol Hwang),김영훈(Young-Hoon Kim),조용석(Yong-Seok Cho),박영식(Young-Shik Park),이재연(Jae-Yeon Lee),강경돈(Kyung-Don Kang),김근(Keun Kim),주동관(Dong-Kwan Joo),안덕균(Duk-Kyun Ahn),성수일(Su-Il Seong) 한국식품영양과학회 2008 한국식품영양과학회지 Vol.37 No.4

당뇨 환자를 위한 기존의 뽕잎 건강식품의 기능을 보완할 목적으로 뽕잎분말과 DNJ 생산균주인 Bacillus subtilis MORI균의 대두발효 혼합물을 신생 당뇨유도쥐에 4주간 경구투여 하여 먹이섭취량, 체중변화, 혈당량, 내당능, 혈중 지질함량, 장기무게 등을 조사하였다. 전체적으로 당뇨유도 시험군은 정상시험군(NC군)과 비교하여 사료섭취량은 많았으나 증체량이 적어 낮은 사료효율을 보였다. 그러나 뽕잎(M군) 및 뽕잎함유대두발효물(MM군)의 투여에 의해 사료효율이 개선되었으며 특히 MM-60군에서 가장 높은 사료효율을 나타내었다. 1주일 간격으로 4주간에 걸쳐 시행한 혈당량 측정 결과 뽕잎이나 뽕잎함유대두발효물의 투여는 공복혈당을 크게 감소시켰으며 특히 MM-60군과 MM-120군은 당뇨대조군(DC군)에 비해 유의적으로 낮게 나타났다. 4주간의 시료투여 후 경구당부하시험에 의한 내당능 조사 결과 뽕잎투여나 뽕잎함유대두발효물의 투여는 혈당량을 꾸준히 감소시켰으며 특히 포도당 투여 후 120분에서의 MM-60군과 MM-120군은 DC군에 비해 유의적으로 낮은 혈당량을 보여 뽕잎 단독보다는 대두발효배양물 첨가에 의해 내당능이 크게 개선된 것으로 나타났다. 혈중 총콜레스테롤 및 중성지질 역시 DC군에서 높은 함량을 나타냈으나 뽕잎이나 뽕잎함유대두발효물 투여에 의해 지질함량이 감소하였으며 그 감량 정도는 총콜레스테롤에서는 MM-60군과 MM-120군에서 그리고 중성지질의 경우에는 M군, MM-60군, MM-120군에서 DC군에 비해 유의적인 감소를 나타내었다. 당뇨 유발에 의해 쥐의 간, 신장, 비장, 심장 등 체내 주요장기의 무게는 크게 증가하였으나 뽕잎 또는 뽕잎함유대두 발효물 투여에 의해 이들 장기의 무게는 대부분 감소하였으며 특히 MM-60군, MM-120군은 DC군에 비해 유의적인 감소를 나타내었다. 이상의 실험결과 뽕잎분말과 DNJ 생산균주인 B. subtilis MORI균의 대두발효물의 혼합물은 신생 당뇨유도쥐의 제반 당뇨증세 완화에 긍정적으로 작용하는 것이 확인되었으며 따라서 뽕잎함유대두발효물은 앞으로 당뇨환자들에 대한 건강식품 소재로 그 활용이 크게 기대된다 하겠다. The effect of fermented soybean culture of Bacillus subtilis MORI mixed with mulberry leaves on the lowering the levels of blood glucose and cholesterol was examined using neonatal streptozotocin-induced diabetic (n-STZ) rats. B. subtilis MORI produces 1-deoxynojirimycin (DNJ), an α-glucosidase inhibitor. The content of DNJ of soybean fermented culture mixed with mulberry was higher (4.1±0.0 ㎎/g dry base) than that (1.5±0.0 ㎎/g) of mulberry. The concentration of 50% inhibition (IC50) against rat intestinal α-glucosidase of soybean fermented culture mixed with mulberry was 5.6±0.1 ㎍/mL and that of mulberry was 17.0±0.5 ㎍/mL. Experimental groups of diabetic rats were randomly assigned to normal control group (NC group), diabetic control group (DC group) and three diabetic groups fed with DNJ food product. One of the three diabetic groups was M group (60 ㎎ DNJ food product/㎏) (DNJ food product containing 30% mulberry) and the other two were MM-60 group (60 ㎎/㎏) and MM-120 group (120 ㎎/㎏) (DNJ-fortified food product containing 30% fermented soybean culture mixed with mulberry leaves). The glucose in serum was significantly decreased in the MM-60 and MM-120 groups fed with DNJ-fortified food product for 4 weeks, compared with DC group. Total cholesterol and triglyceride in serum were also lower in MM-60 and MM-120 groups than the DC group. These results support that the fermented soybean culture of B. subtilis MORI mixed with mulberry leaves improved the metabolism of blood glucose and lipid in the n-STZ rat model.

신규 통정혐기정 세균으로 제조한 발효흙에 의한 음식물 쓰레기의 퇴비화

황교열(Kyo-Yeol Hwang),이재연(Jae-Yeon Lee),김근(Keun Kim),성수일(Su-Il Sung),한승호(Sung-Ho Han) 유기성자원학회 2001 유기물자원화 Vol.9 No.1

음식물쓰레기 퇴비화를 위한 발효흙 제조를 위하여 토양에서 새로 분리한 Bacillus 속의 GM103, V25, V31, V35 의 4균주를 사용하였다 각 균주를 통정한 결과 각각 Bacillus licheniformis, B. subti1is, B.stearothennophilius, B. subtilis로 동정되었다 이 들 균주들은 단백질 분해능, 전분분해능, 그리고 작물 병원성 곰팡이 Rhizopus stronifeI에 대한 저해능이 모두 우수하였다. GM103은 전분분해능이 탁월하게 우수하였고, 호기적 성장만 가능하였다. V25, V31, V35는 모두 호기적 • 혐기적 성장이 가능하였고, 10% 염분농도와 50℃에서 좋은 성장도를 보였으며 토양에서의 생존 및 적응력도 우수하였다. 음식물쓰레기 퇴비화 시험을 위하여 GM103, V25, V31, V35 균주를 배양하여 당밀, 비트펄프, zeolite 등을 혼합하여 발효흙인 BIOTOP-CLEAN을제조하였다. 제조된 BIOTOP-CLEAN과 무처리구인 대조구 기존 타사 제품 HS와 음식물쓰레기 시험을 하였는데 대조구의 30'C , HS의 35'C 보다 BIOTOP-CLEAN의 경우가 최대 발효온도는 50'C로 가장 높았다. 또한 BIOTOP-CLEAN은 냄새도 구수하였고 성상에 있어서도 짙은 암갈색으로 음식물쓰레기의 퇴비화가 가장 잘 되었다. 한편 대조구는 악취가 나고 HS는 별다른 냄새가 없었다. 각 균주의 배양액을 토마토, 배추, 열무, 고추 등의 작물에 1달주기로 살포하여 무처리인 대조구에 비해 상대적 증산율에서 모두 우수한 것으로 나타났다. Four newly isolated bacteria from soil were used manufacture microbial inoculum food waste. The baaeria, GM103, V25, V31, and V35, were identifed as Bacillus licheniformis, B.sutilis, B. stearothermophilus and B. subtiIis, respectively. The bacterial Strains were effcient to degrade protein and srarch and also able to inhibit the growth of plant pathogenic fungus Rhizopus strinifer. The GM103 showed distinct capability in degrading srarch, but grow only aerobically. The other three bacterial strains, V25, V31, and V35, could grow both aerobicallyas well as anaerobically, in lO%(w/v) salt, at 50'C, and had good viabiliry and survival rate in soil. These characteristics of the bacterial Strains are very adquate in Korean food composting containing high concenrration of salt, especially at home. By mixing the 4 bacterial culrure broth with molasses, beet pulp, zeolite, The bacterial for food waste composting BIOTOP-CLEAN-was made. The perfolll ance of fool waste composting by the BIOTOP-CLEAN was compared with that by control(not treated) and HS(other domestic company’s inoculum produa for food waste composting). The maximum temperature of the food waste during the co,posting with the BIOTOP-CLEAN was 50C, while those of the control gave bad smell and HS gave less bad smell. These indicater that the food waste composting by the BIOTOP-CLEAN had been we;; accomplished.

Partial sublingual glandectomy with ranula excision: a new conservative method for treatment

In-Kyo Chung,Hyo-Ji Lee,Dae-Seok Hwang,Yong-Deok Kim,Hae-Ryoun Park,Sang-Hun Shin,Uk-Kyu Kim,Jae-Yeol Lee 대한구강악안면외과학회 2012 대한구강악안면외과학회지 Vol.38 No.3

Objectives: This study evaluated the clinical results of partial sublingual glandectomy accompanying the excision of ranula as new treatment modality. Materials and Methods: A total of 43 patients who were treated between 1999 and 2007 for oral or plunging ranula were reviewed. All patients were treated surgically by various methods with a total of 55 different procedures performed. Ten cases of partial sublingual glandectomy with excision of the ranula were conducted. All excised specimens were examined. We compared the clinical outcomes resulting from each treatment method. Results: The recurrence rates for marsupialization, excision of ranula, marsupialization with gauze packing, total excision of sublingual gland and ranula, and partial sublingual glandectomy with excision of ranula were 50%, 25%, 25%, 0% and 10%, respectively. Of the 10 patients treated by partial sublingual glandectomy with ranula excision, only one experienced recurrence (10%), i.e., plunging ranula. None of the ranulas contained an epithelial lining, and the excised portion of the feeding sublingual glands showed degenerative changes. Conclusion: In removal of ranulas, we found that excision of the attached sublingual gland, which removed the feeding portion and degenerative acinar cells, yielded good outcomes. Thus, as a new conservative method for treatment, we recommend partial sublingual glandectomy to accompany excision of the ranula.

태양광-전력저장장치 연계운영의 최대부하 이동에 관한 연구

안교상(Kyo-Sang Ahn),김경환(Kyeong-Hwan Kim),한경태(Kyung-Tai Han),정순열(Soon-Yeol Jeong),황우현(Woo-Hyun Hwang) 대한전기학회 2010 대한전기학회 학술대회 논문집 Vol.2010 No.7

전력계통의 전력부하 상태는 일일 혹은 계절별로 다양한 형태를 보여주고 있다. 산업의 발전과 더블어 생활문화의 향상은 전기에너지 소비의 촉진을 가져오고 사용되는 전력부하는 다양한 패턴으로 최대-최저부하의 곡선을 그리며, 전력계통 운영에 직/간접적으로 영향을 주고 있다. 이에 본 고에서는 청정한 발전전원인 태양광발전과 전력저장장치를 적용하여 최대전력부하 이동에 따른 전력회사 혹은 소비자 측면에서 운영에 따른 특성과 계통의 영향에 대하여 논하고자 한다.

식도확장이 뚜렷하지 않은 아칼라지아

추교영,최명규,박성규,이성희,최황,박수현,한준열,김재광,한석원,정인식,정규원,선희식 대한소화관운동학회 1999 Journal of Neurogastroenterology and Motility (JNM Vol.5 No.2

안면 비대칭환자의 하악골체부의 시상 단면에 관한 연구

이재열(Jae-Yeol Lee),김용일(Yong-Il Kim),황대석(Dae-Seok Hwang),김용덕(Yong-Deok Kim),신상훈(Sang-Hun Shin),김욱규(Uk-Kyu Kim),정인교(In-Kyo Chung) 대한구강악안면외과학회 2011 대한구강악안면외과학회지 Vol.37 No.2

Introduction: To correct the facial asymmetry by mandibular jaw surgery, it is important to know the anatomy of the mandible including the mandibular canal positioning of patients with facial asymmetry. This study was performed to evaluate the differences in the cross-sectional surface in the body of the mandible between the deviated side and opposite side in patients with facial asymmetry. Materials and Methods: The study was conducted on 37 adult patients composed of 2 groups, the asymmetry group (n=20) and non-asymmetry group (n=17). Using the cross-sectional computed tomography (CT) images, the distance from the buccal aspect of the mandibular canal to the outer aspect of the buccal cortex, distance from the buccal aspect of the mandibular canal to the inner aspect of the buccal cortex, distance from the inferior aspect of the mandibular canal to the inferior border of the mandible, thickness of the mandible, and cross-sectional surface area of the mandible were measured in each side of the mandible. Results: The cross-sectional area of the mandible including the mandibular canal positioning in the deviated side was not statistically different from the opposite side in the asymmetry group. Only the distance from the inferior aspect of the mandibular canal to the inferior border of the mandible in the ramus area of the deviated side was significantly longer than opposite side. On the other hand, the bucco-lingual width of the asymmetry group was thinner than the non-asymmetry group. Conclusion: The cross-sectional area including the mandibular canal of the mandible did not appear to be modified by the facial asymmetry.

A Study on Life Expectancy Forecast for Porcelain Insulators

이원교(Won Kyo Lee),최인혁(In-Hyuk Choi),이동일,최한열(Han Yeol Choi),Kab Cheol Hwang 대한전기학회 2008 대한전기학회 학술대회 논문집 Vol.2008 No.7(초록집)

폴리머 애자의 산불 신뢰성 평가 연구

이원교(Won Kyo Lee),최인혁(In-Hyuk Choi),황갑철(Kab Cheol Hwang),최한열(Han Yeol Choi) 대한전기학회 2008 대한전기학회 학술대회 논문집 Vol.2008 No.7

상안와벽 및 상안와연 골절의 치험례

이재열(Jae-Yeol Lee),이성탁(Sung-Tak Lee),김용덕(Yong-Deok Kim),신상훈(Sang-Hun Shin),김욱규(Uk-Kyu Kim),정인교(In-Kyo Chung),황대석(Dae-Seok Hwang) 대한구강악안면외과학회 2011 대한구강악안면외과학회지 Vol.37 No.6

Orbital roof and supraorbital ridge fractures are frequently associated with high energy concomitant craniofacial trauma. When a displacement of the orbital roof and supraorbital rim occurs, exploration, stabilization and reconstruction are warranted to limit the ocular complications. The management of fractures involving the frontal sinus must consider the possible need for obliteration or cranialization of the sinus. Many incisions have been described and used to approach these fractures, such as a coronal incision, eyebrow incision, and an incision through the laceration. We report 3 cases of orbital roof and supraorbital ridge fracture patients with a review of the relevant literature.